4iq4 - Revision history

OCA at 15:28, 20 September 2023

2023-09-20T15:28:45Z

←Older revision		Revision as of 15:28, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4iq4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Puerto_Rico/8/1934(H1N1)) Influenza A virus (A/Puerto Rico/8/1934(H1N1))] and [https://en.wikipedia.org/wiki/Kitasatospora_aureofaciens Kitasatospora aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IQ4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4iq4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Puerto_Rico/8/1934(H1N1)) Influenza A virus (A/Puerto Rico/8/1934(H1N1))] and [https://en.wikipedia.org/wiki/Kitasatospora_aureofaciens Kitasatospora aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IQ4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iq4 OCA], [https://pdbe.org/4iq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iq4 RCSB], [https://www.ebi.ac.uk/pdbsum/4iq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iq4 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.495Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iq4 OCA], [https://pdbe.org/4iq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iq4 RCSB], [https://www.ebi.ac.uk/pdbsum/4iq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iq4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 21:03, 16 November 2022

2022-11-16T21:03:02Z

←Older revision		Revision as of 21:03, 16 November 2022
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	==Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P21212 form==		==Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P21212 form==
-	<StructureSection load='4iq4' size='340' side='right' caption='[[4iq4]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<StructureSection load='4iq4' size='340' side='right'caption='[[4iq4]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4iq4]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_10762 Atcc 10762~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IQ4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4IQ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4iq4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Puerto_Rico/8/1934(H1N1)) Influenza A virus (A/Puerto Rico/8/1934(H1N1))] and [https://en.wikipedia.org/wiki/Kitasatospora_aureofaciens Kitasatospora aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IQ4 FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vdx\|3vdx]], [[4d9j\|4d9j]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iq4 OCA], [https://pdbe.org/4iq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iq4 RCSB], [https://www.ebi.ac.uk/pdbsum/4iq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iq4 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpoA2, BROMOPEROXIDASE A2 and M1 Matrix fusion, M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ATCC 10762])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4iq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iq4 OCA], [~~http~~://pdbe.org/4iq4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4iq4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4iq4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4iq4 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~BPOA2_STRAU BPOA2_STRAU]~~] May be a chlorinating enzyme involved in 7-chlorotetracycline biosynthesis.	+	[https://www.uniprot.org/uniprot/BPOA2_KITAU BPOA2_KITAU] May be a chlorinating enzyme involved in 7-chlorotetracycline biosynthesis.[https://www.uniprot.org/uniprot/M1_I34A1 M1_I34A1] Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place.<ref>PMID:11531417</ref> Determines the virion's shape: spherical or filamentous. Clinical isolates of influenza are characterized by the presence of significant proportion of filamentous virions, whereas after multiple passage on eggs or cell culture, virions have only spherical morphology. Filamentous virions are thought to be important to infect neighboring cells, and spherical virions more suited to spread through aerosol between hosts organisms.<ref>PMID:11531417</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4iq4" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4iq4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Haloperoxidase\|Haloperoxidase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 10762~~]]	+	[[Category: Kitasatospora aureofaciens]]
-	[[Category: ~~Lai, Y T~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Sawaya, M R]]~~	+	[[Category: Lai Y-T]]
-	~~[[Category: Yeates,~~ T O]]	+	[[Category: Sawaya MR]]
-	[[Category: ~~Biomaterial~~]]	+	[[Category: Yeates TO]]
-	[[Category: ~~Bionanotechnology]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Protein assembly]]~~	+
-	~~[[Category: Protein design]]~~	+
-	~~[[Category: Symmetric oligomeric domain~~]]	+

OCA at 15:06, 4 August 2016

2016-08-04T15:06:16Z

←Older revision		Revision as of 15:06, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P21212 form==		==Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P21212 form==
	<StructureSection load='4iq4' size='340' side='right' caption='[[4iq4]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='4iq4' size='340' side='right' caption='[[4iq4]], [[Resolution\|resolution]] 3.50Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vdx\|3vdx]], [[4d9j\|4d9j]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vdx\|3vdx]], [[4d9j\|4d9j]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpoA2, BROMOPEROXIDASE A2 and M1 Matrix fusion, M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ATCC 10762])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpoA2, BROMOPEROXIDASE A2 and M1 Matrix fusion, M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ATCC 10762])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iq4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iq4 RCSB], [http://www.ebi.ac.uk/pdbsum/4iq4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iq4 OCA], [http://pdbe.org/4iq4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4iq4 RCSB], [http://www.ebi.ac.uk/pdbsum/4iq4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4iq4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4iq4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:06, 24 December 2014

2014-12-24T16:06:33Z

←Older revision		Revision as of 16:06, 24 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4iq4\| PDB=4iq4 \| SCENE= }}~~	+	==Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P21212 form==
-	===Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P21212 form===	+	<StructureSection load='4iq4' size='340' side='right' caption='[[4iq4]], [[Resolution\|resolution]] 3.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23621606}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4iq4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10762 Atcc 10762]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IQ4 FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vdx\|3vdx]], [[4d9j\|4d9j]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpoA2, BROMOPEROXIDASE A2 and M1 Matrix fusion, M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ATCC 10762])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iq4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iq4 RCSB], [http://www.ebi.ac.uk/pdbsum/4iq4 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/BPOA2_STRAU BPOA2_STRAU]] May be a chlorinating enzyme involved in 7-chlorotetracycline biosynthesis.		[[http://www.uniprot.org/uniprot/BPOA2_STRAU BPOA2_STRAU]] May be a chlorinating enzyme involved in 7-chlorotetracycline biosynthesis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Designing protein molecules that self-assemble into complex architectures is an outstanding goal in the area of nanobiotechnology. One design strategy for doing this involves genetically fusing together two natural proteins, each of which is known to form a simple oligomer on its own (e.g., a dimer or trimer). If two such components can be fused in a geometrically predefined configuration, that designed subunit can, in principle, assemble into highly symmetric architectures. Initial experiments showed that a 12-subunit tetrahedral cage, 16 nm in diameter, could be constructed following such a procedure [Padilla, J. E.; et al. Proc. Natl. Acad. Sci. U.S.A. 2001, 98, 2217; Lai, Y. T.; et al. Science 2012, 336, 1129]. Here we characterize multiple crystal structures of protein cages constructed in this way, including cages assembled from two mutant forms of the same basic protein subunit. The flexibilities of the designed assemblies and their deviations from the target model are described, along with implications for further design developments.

-	~~==About this~~ Structure==	+	Structure and flexibility of nanoscale protein cages designed by symmetric self-assembly.,Lai YT, Tsai KL, Sawaya MR, Asturias FJ, Yeates TO J Am Chem Soc. 2013 May 22;135(20):7738-43. doi: 10.1021/ja402277f. Epub 2013 May, 8. PMID:23621606<ref>PMID:23621606</ref>
-	~~[[4iq4]] is a 6 chain structure~~. ~~Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4IQ4 OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023621606</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
-	[[Category: Lai, Y T.]]	+	== References ==
-	[[Category: Sawaya, M R.]]	+	<references/>
-	[[Category: Yeates, T O.]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Atcc 10762]]
		+	[[Category: Lai, Y T]]
		+	[[Category: Sawaya, M R]]
		+	[[Category: Yeates, T O]]
	[[Category: Biomaterial]]		[[Category: Biomaterial]]
	[[Category: Bionanotechnology]]		[[Category: Bionanotechnology]]

OCA at 12:51, 24 July 2013

2013-07-24T12:51:43Z

←Older revision		Revision as of 12:51, 24 July 2013
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-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4iq4\| PDB=4iq4 \| SCENE= }}
		+	===Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P21212 form===
		+	{{ABSTRACT_PUBMED_23621606}}

-	~~The entry 4iq4 is ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/BPOA2_STRAU BPOA2_STRAU]] May be a chlorinating enzyme involved in 7-chlorotetracycline biosynthesis.

-	~~Authors~~: ~~Lai, Y~~.-T.~~, Sawaya, M~~.~~R., Yeates, T.O~~.	+	==About this Structure==
		+	[[4iq4]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IQ4 OCA].

-	~~Description~~: ~~Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains~~, ~~triple mutant~~, ~~P21212 form~~	+	==Reference==
		+	<ref group="xtra">PMID:023621606</ref><references group="xtra"/><references/>
		+	[[Category: Lai, Y T.]]
		+	[[Category: Sawaya, M R.]]
		+	[[Category: Yeates, T O.]]
		+	[[Category: Biomaterial]]
		+	[[Category: Bionanotechnology]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Protein assembly]]
		+	[[Category: Protein design]]
		+	[[Category: Symmetric oligomeric domain]]

OCA at 14:19, 27 February 2013

2013-02-27T14:19:51Z

←Older revision		Revision as of 14:19, 27 February 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4iq4 is ON HOLD	+	The entry 4iq4 is ON HOLD until Paper Publication

	Authors: Lai, Y.-T., Sawaya, M.R., Yeates, T.O.		Authors: Lai, Y.-T., Sawaya, M.R., Yeates, T.O.

	Description: Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P21212 form		Description: Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P21212 form

OCA at 11:13, 23 January 2013

2013-01-23T11:13:03Z

←Older revision		Revision as of 11:13, 23 January 2013
Line 3:		Line 3:
	The entry 4iq4 is ON HOLD		The entry 4iq4 is ON HOLD

-	Authors: Lai, ~~Yen~~-~~Ting~~, Sawaya, ~~Michael~~ R., Yeates, ~~Todd~~ O.	+	Authors: Lai, Y.-T., Sawaya, M.R., Yeates, T.O.

	Description: Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P21212 form		Description: Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P21212 form

OCA: Protected "4iq4" [edit=sysop:move=sysop]

2013-01-16T08:29:41Z

Protected "4iq4" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:29, 16 January 2013

OCA: New page: '''Unreleased structure''' The entry 4iq4 is ON HOLD Authors: Lai, Yen-Ting, Sawaya, Michael R., Yeates, Todd O. Description: Structure of a 16 nm protein cage designed by fusing symme...

2013-01-16T08:29:40Z

New page: '''Unreleased structure''' The entry 4iq4 is ON HOLD Authors: Lai, Yen-Ting, Sawaya, Michael R., Yeates, Todd O. Description: Structure of a 16 nm protein cage designed by fusing symme...

New page

'''Unreleased structure'''

The entry 4iq4 is ON HOLD

Authors: Lai, Yen-Ting, Sawaya, Michael R., Yeates, Todd O.

Description: Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, triple mutant, P21212 form