4iu2 - Revision history

OCA at 21:11, 16 November 2022

2022-11-16T21:11:01Z

←Older revision		Revision as of 21:11, 16 November 2022
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	==Cohesin-dockerin -X domain complex from Ruminococcus flavefacience==		==Cohesin-dockerin -X domain complex from Ruminococcus flavefacience==
-	<StructureSection load='4iu2' size='340' side='right' caption='[[4iu2]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4iu2' size='340' side='right'caption='[[4iu2]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4iu2]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_19208 Atcc 19208~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IU2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4IU2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4iu2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruminococcus_flavefaciens Ruminococcus flavefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IU2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4IU2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=~~SO4~~:~~SULFATE+ION~~'>~~SO4~~</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4iu2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iu2 OCA], [https://pdbe.org/4iu2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4iu2 RCSB], [https://www.ebi.ac.uk/pdbsum/4iu2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4iu2 ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4iu3\|4iu3]]</td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">scaE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1265 ATCC 19208]), cttA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1265 ATCC 19208])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4iu2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iu2 OCA], [~~http~~://pdbe.org/4iu2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4iu2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4iu2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4iu2 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0AEF6_RUMFL A0AEF6_RUMFL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 19208~~]]	+	[[Category: Large Structures]]
-	[[Category: Bayer, E]]	+	[[Category: Ruminococcus flavefaciens]]
-	[[Category: Frolow, F]]	+	[[Category: Bayer E]]
-	[[Category: Salama-Alber, O]]	+	[[Category: Frolow F]]
-	~~[[Category: Beta sandwich]]~~	+	[[Category: Salama-Alber O]]
-	~~[[Category: Cellulose degradation]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 13:45, 4 August 2016

2016-08-04T13:45:17Z

←Older revision		Revision as of 13:45, 4 August 2016
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		+
	==Cohesin-dockerin -X domain complex from Ruminococcus flavefacience==		==Cohesin-dockerin -X domain complex from Ruminococcus flavefacience==
	<StructureSection load='4iu2' size='340' side='right' caption='[[4iu2]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4iu2' size='340' side='right' caption='[[4iu2]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4iu2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Ruminococcus_flavefaciens Ruminococcus flavefaciens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IU2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IU2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4iu2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19208 Atcc 19208]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IU2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IU2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4iu3\|4iu3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4iu3\|4iu3]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">scaE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1265 ~~Ruminococcus flavefaciens~~]), cttA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1265 ~~Ruminococcus flavefaciens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">scaE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1265 ATCC 19208]), cttA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1265 ATCC 19208])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iu2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iu2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iu2 RCSB], [http://www.ebi.ac.uk/pdbsum/4iu2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iu2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iu2 OCA], [http://pdbe.org/4iu2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4iu2 RCSB], [http://www.ebi.ac.uk/pdbsum/4iu2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4iu2 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4iu2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ruminococcus flavefaciens~~]]	+	[[Category: Atcc 19208]]
	[[Category: Bayer, E]]		[[Category: Bayer, E]]
	[[Category: Frolow, F]]		[[Category: Frolow, F]]

OCA at 12:31, 21 December 2014

2014-12-21T12:31:23Z

←Older revision		Revision as of 12:31, 21 December 2014
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-	~~{{STRUCTURE_4iu2\| PDB=4iu2 \| SCENE= }}~~	+	==Cohesin-dockerin -X domain complex from Ruminococcus flavefacience==
-	===Cohesin-dockerin -X domain complex from Ruminococcus flavefacience===	+	<StructureSection load='4iu2' size='340' side='right' caption='[[4iu2]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23580648}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4iu2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ruminococcus_flavefaciens Ruminococcus flavefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IU2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IU2 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4iu3\|4iu3]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">scaE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1265 Ruminococcus flavefaciens]), cttA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1265 Ruminococcus flavefaciens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4iu2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4iu2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4iu2 RCSB], [http://www.ebi.ac.uk/pdbsum/4iu2 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The rumen bacterium Ruminococcus flavefaciens produces a highly organized multienzyme cellulosome complex that plays a key role in the degradation of plant cell wall polysaccharides, notably cellulose. The R. flavefaciens cellulosomal system is anchored to the bacterial cell wall through a relatively small ScaE scaffoldin subunit, which bears a single type-IIIe cohesin responsible for the attachment of two major dockerin-containing scaffoldin proteins: ScaB and the cellulose-binding protein CttA. While ScaB recruits the catalytic machinery onto the complex, CttA mediates attachment of the bacterial substrate via its two putative carbohydrate-binding modules (CBMs). In an effort to understand the structural basis for assembly and cell-surface attachment of the cellulosome in R. flavefaciens, we determined the crystal structure of the high-affinity complex (Kd = 20.83 nM) between the ScaE cohesin module (CohE) and its cognate X-dockerin modular dyad (XDoc) from CttA at 1.97-A resolution. The structure reveals an atypical calcium-binding loop containing a 13-residue insert. The results further pinpoint two charged specificity-related residues on the surface of the cohesin module, which are responsible for specific vs. promiscuous cross-strain binding of the dockerin module. In addition, a combined functional role for the three enigmatic dockerin inserts was established, whereby these extraneous segments serve as structural buttresses that reinforce the stalk-like conformation of the X-module, thus segregating its tethered complement of cellulosomal components from the cell surface. The novel structure of the RfCohE-XDoc complex sheds light on divergent dockerin structure and function and provides insight into the specificity features of the type-IIIe cohesin-dockerin interaction.

-	~~==About this Structure==~~	+	Atypical cohesin-dockerin complex responsible for cell-surface attachment of cellulosomal components: binding fidelity, promiscuity, and structural buttresses.,Salama-Alber O, Jobby MK, Chitayat S, Smith SP, White BA, Shimon LJ, Lamed R, Frolow F, Bayer EA J Biol Chem. 2013 Apr 11. PMID:23580648<ref>PMID:23580648</ref>
-	~~[[4iu2]] is a 2 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Ruminococcus_flavefaciens Ruminococcus flavefaciens]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4IU2 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023580648</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Ruminococcus flavefaciens]]		[[Category: Ruminococcus flavefaciens]]
-	[[Category: Bayer, E.]]	+	[[Category: Bayer, E]]
-	[[Category: Frolow, F.]]	+	[[Category: Frolow, F]]
-	[[Category: Salama-Alber, O.]]	+	[[Category: Salama-Alber, O]]
	[[Category: Beta sandwich]]		[[Category: Beta sandwich]]
	[[Category: Cellulose degradation]]		[[Category: Cellulose degradation]]
	[[Category: Structural protein]]		[[Category: Structural protein]]

OCA at 12:26, 3 July 2013

2013-07-03T12:26:30Z

←Older revision		Revision as of 12:26, 3 July 2013
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	==About this Structure==		==About this Structure==
	[[4iu2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ruminococcus_flavefaciens Ruminococcus flavefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IU2 OCA].		[[4iu2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ruminococcus_flavefaciens Ruminococcus flavefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IU2 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023580648</ref><references group="xtra"/><references/>
	[[Category: Ruminococcus flavefaciens]]		[[Category: Ruminococcus flavefaciens]]
	[[Category: Bayer, E.]]		[[Category: Bayer, E.]]

OCA at 11:20, 24 April 2013

2013-04-24T11:20:00Z

←Older revision		Revision as of 11:20, 24 April 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4iu2\| PDB=4iu2 \| SCENE= }}
		+	===Cohesin-dockerin -X domain complex from Ruminococcus flavefacience===
		+	{{ABSTRACT_PUBMED_23580648}}

-	~~The entry~~ 4iu2 is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4iu2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ruminococcus_flavefaciens Ruminococcus flavefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IU2 OCA].
-	~~Authors~~: ~~Salama~~-~~Alber, O~~., Bayer, E., Frolow, F.	+	[[Category: Ruminococcus flavefaciens]]
-		+	[[Category: Bayer, E.]]
-	~~Description~~: ~~Cohesin~~-~~dockerin -X domain complex from Ruminococcus flavefacience~~	+	[[Category: Frolow, F.]]
		+	[[Category: Salama-Alber, O.]]
		+	[[Category: Beta sandwich]]
		+	[[Category: Cellulose degradation]]
		+	[[Category: Structural protein]]

OCA at 13:46, 6 March 2013

2013-03-06T13:46:51Z

←Older revision		Revision as of 13:46, 6 March 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4iu2 is ON HOLD	+	The entry 4iu2 is ON HOLD until Paper Publication

	Authors: Salama-Alber, O., Bayer, E., Frolow, F.		Authors: Salama-Alber, O., Bayer, E., Frolow, F.

	Description: Cohesin-dockerin -X domain complex from Ruminococcus flavefacience		Description: Cohesin-dockerin -X domain complex from Ruminococcus flavefacience

OCA at 10:06, 13 February 2013

2013-02-13T10:06:51Z

←Older revision		Revision as of 10:06, 13 February 2013
Line 3:		Line 3:
	The entry 4iu2 is ON HOLD		The entry 4iu2 is ON HOLD

-	Authors: ~~Orly~~ Salama-Alber, Ed Bayer, ~~Felix~~ Frolow	+	Authors: Salama-Alber, O., Bayer, E., Frolow, F.

	Description: Cohesin-dockerin -X domain complex from Ruminococcus flavefacience		Description: Cohesin-dockerin -X domain complex from Ruminococcus flavefacience

OCA: Protected "4iu2" [edit=sysop:move=sysop]

2013-01-30T12:04:43Z

Protected "4iu2" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:04, 30 January 2013

OCA: New page: '''Unreleased structure''' The entry 4iu2 is ON HOLD Authors: Orly Salama-Alber, Ed Bayer, Felix Frolow Description: Cohesin-dockerin -X domain complex from Ruminococcus flavefacience

2013-01-30T12:04:42Z

New page: '''Unreleased structure''' The entry 4iu2 is ON HOLD Authors: Orly Salama-Alber, Ed Bayer, Felix Frolow Description: Cohesin-dockerin -X domain complex from Ruminococcus flavefacience

New page

'''Unreleased structure'''

The entry 4iu2 is ON HOLD

Authors: Orly Salama-Alber, Ed Bayer, Felix Frolow

Description: Cohesin-dockerin -X domain complex from Ruminococcus flavefacience