4j5n - Revision history

OCA at 15:36, 20 September 2023

2023-09-20T15:36:18Z

←Older revision		Revision as of 15:36, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4j5n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J5N FirstGlance]. <br>		<table><tr><td colspan='2'>[[4j5n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J5N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.05Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5n OCA], [https://pdbe.org/4j5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j5n RCSB], [https://www.ebi.ac.uk/pdbsum/4j5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j5n ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5n OCA], [https://pdbe.org/4j5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j5n RCSB], [https://www.ebi.ac.uk/pdbsum/4j5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j5n ProSAT]</span></td></tr>
	</table>		</table>

OCA at 21:33, 16 November 2022

2022-11-16T21:33:46Z

←Older revision		Revision as of 21:33, 16 November 2022
Line 1:		Line 1:

	==Crystal Structure of a Deinococcus radiodurans PTE-like lactonase (drPLL) mutant Y28L/D71N/E101G/E179D/V235L/L270M==		==Crystal Structure of a Deinococcus radiodurans PTE-like lactonase (drPLL) mutant Y28L/D71N/E101G/E179D/V235L/L270M==
-	<StructureSection load='4j5n' size='340' side='right' caption='[[4j5n]], [[Resolution\|resolution]] 2.05Å' scene=''>	+	<StructureSection load='4j5n' size='340' side='right'caption='[[4j5n]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4j5n]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus~~ radiodurans~~" raj et al. 1960~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4J5N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4j5n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4J5N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4j5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5n OCA], [https://pdbe.org/4j5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4j5n RCSB], [https://www.ebi.ac.uk/pdbsum/4j5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4j5n ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dr0930 (gi 15805954), DR_0930 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 "Micrococcus radiodurans" Raj et al. 1960])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4j5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5n OCA], [~~http~~://pdbe.org/4j5n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4j5n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4j5n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4j5n ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9RVU2_DEIRA Q9RVU2_DEIRA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Micrococcus~~ radiodurans ~~raj et al. 1960~~]]	+	[[Category: Deinococcus radiodurans]]
-	[[Category: Barondeau~~, D P~~]]	+	[[Category: Large Structures]]
-	[[Category: Fox~~, N G~~]]	+	[[Category: Barondeau DP]]
-	[[Category: Hocker, B]]	+	[[Category: Fox NG]]
-	[[Category: Malisi, C]]	+	[[Category: Hocker B]]
-	[[Category: Meier~~, M M~~]]	+	[[Category: Malisi C]]
-	[[Category: Rajendran, C]]	+	[[Category: Meier MM]]
-	[[Category: Raushel~~, F M~~]]	+	[[Category: Rajendran C]]
-	[[Category: Schlee, S]]	+	[[Category: Raushel FM]]
-	[[Category: Sterner, R]]	+	[[Category: Schlee S]]
-	[[Category: Xu, C]]	+	[[Category: Sterner R]]
-	~~[[Category: Hydrolase]]~~	+	[[Category: Xu C]]
-	~~[[Category: Lactonase]]~~	+
-	~~[[Category: Metalloenzyme]]~~	+
-	~~[[Category: Nerve agent]]~~	+
-	~~[[Category: Phosphotriesterase]]~~	+
-	~~[[Category: Tim barrel~~]]	+

OCA at 08:02, 11 August 2016

2016-08-11T08:02:17Z

←Older revision		Revision as of 08:02, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of a Deinococcus radiodurans PTE-like lactonase (drPLL) mutant Y28L/D71N/E101G/E179D/V235L/L270M==		==Crystal Structure of a Deinococcus radiodurans PTE-like lactonase (drPLL) mutant Y28L/D71N/E101G/E179D/V235L/L270M==
	<StructureSection load='4j5n' size='340' side='right' caption='[[4j5n]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='4j5n' size='340' side='right' caption='[[4j5n]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4j5n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Deinococcus_radiodurans Deinococcus~~ radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J5N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4j5n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J5N FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dr0930 (gi 15805954), DR_0930 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 ~~Deinococcus~~ radiodurans])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dr0930 (gi 15805954), DR_0930 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 "Micrococcus radiodurans" Raj et al. 1960])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j5n RCSB], [http://www.ebi.ac.uk/pdbsum/4j5n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5n OCA], [http://pdbe.org/4j5n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4j5n RCSB], [http://www.ebi.ac.uk/pdbsum/4j5n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4j5n ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4j5n" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Deinococcus~~ radiodurans]]	+	[[Category: Micrococcus radiodurans raj et al. 1960]]
	[[Category: Barondeau, D P]]		[[Category: Barondeau, D P]]
	[[Category: Fox, N G]]		[[Category: Fox, N G]]

OCA at 13:12, 5 January 2015

2015-01-05T13:12:08Z

←Older revision		Revision as of 13:12, 5 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4j5n\| PDB=4j5n \| SCENE= }}~~	+	==Crystal Structure of a Deinococcus radiodurans PTE-like lactonase (drPLL) mutant Y28L/D71N/E101G/E179D/V235L/L270M==
-	===Crystal Structure of a Deinococcus radiodurans PTE-like lactonase (drPLL) mutant Y28L/D71N/E101G/E179D/V235L/L270M===	+	<StructureSection load='4j5n' size='340' side='right' caption='[[4j5n]], [[Resolution\|resolution]] 2.05Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23837603}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4j5n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J5N FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dr0930 (gi 15805954), DR_0930 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 Deinococcus radiodurans])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j5n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j5n RCSB], [http://www.ebi.ac.uk/pdbsum/4j5n PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rapid evolution of enzymes provides unique molecular insights into the remarkable adaptability of proteins and helps to elucidate the relationship between amino acid sequence, structure and function. We interrogated the evolution of the phosphotriesterase from Pseudomonas diminuta (PdPTE), which hydrolyzes synthetic organophosphates with remarkable catalytic efficiency. PTE is thought to be an evolutionarily "young" enzyme and it has been postulated that it has evolved from members of the phosphotriesterase-like lactonase (PLL) family that show promiscuous organophosphate degrading activity. Starting from a weakly promiscuous PLL scaffold (Dr0930 from Deinococcus radiodurans), we designed an extremely efficient organophosphate hydrolase (OPH) with broad substrate specificity using rational and random mutagenesis in combination with in vitro activity screening. The OPH activity for seven organophosphate substrates was simultaneously enhanced by up to five orders of magnitude, achieving absolute values of catalytic efficiencies up to 106 M-1 s-1. Structural and computational analyses identified the molecular basis for the enhanced OPH activity of the engineered PLL variants and demonstrated that OPH catalysis in PdPTE and the engineered PLL differ significantly in the mode of substrate binding.

-	~~==About this Structure==~~	+	Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template.,Meier MM, Rajendran C, Malisi C, Fox NG, Xu C, Schlee S, Barondeau DP, Hoecker B, Sterner R, Raushel FM J Am Chem Soc. 2013 Jul 9. PMID:23837603<ref>PMID:23837603</ref>
-	~~[[4j5n]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4J5N OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023837603</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Deinococcus radiodurans]]		[[Category: Deinococcus radiodurans]]
-	[[Category: Barondeau, D P.]]	+	[[Category: Barondeau, D P]]
-	[[Category: Fox, N G.]]	+	[[Category: Fox, N G]]
-	[[Category: Hocker, B.]]	+	[[Category: Hocker, B]]
-	[[Category: Malisi, C.]]	+	[[Category: Malisi, C]]
-	[[Category: Meier, M M.]]	+	[[Category: Meier, M M]]
-	[[Category: Rajendran, C.]]	+	[[Category: Rajendran, C]]
-	[[Category: Raushel, F M.]]	+	[[Category: Raushel, F M]]
-	[[Category: Schlee, S.]]	+	[[Category: Schlee, S]]
-	[[Category: Sterner, R.]]	+	[[Category: Sterner, R]]
-	[[Category: Xu, C.]]	+	[[Category: Xu, C]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Lactonase]]		[[Category: Lactonase]]

OCA at 12:57, 24 July 2013

2013-07-24T12:57:08Z

←Older revision		Revision as of 12:57, 24 July 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4j5n\| PDB=4j5n \| SCENE= }}
		+	===Crystal Structure of a Deinococcus radiodurans PTE-like lactonase (drPLL) mutant Y28L/D71N/E101G/E179D/V235L/L270M===
		+	{{ABSTRACT_PUBMED_23837603}}

-	~~The entry~~ 4j5n is ~~ON HOLD~~	+	==About this Structure==
		+	[[4j5n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J5N OCA].

-	~~Authors~~: ~~Meier~~, ~~M.M~~., ~~Rajendran, C~~., Malisi, C., ~~Fox, N.G~~.~~, Xu~~, C., Schlee, S.~~, Barondeau, D.P., Hocker, B.,~~ Sterner, R., ~~Raushel, F.M~~.	+	==Reference==
-		+	<ref group="xtra">PMID:023837603</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal Structure of a Deinococcus radiodurans PTE-like lactonase (drPLL) mutant Y28L/D71N/E101G/E179D/V235L/L270M~~	+	[[Category: Deinococcus radiodurans]]
		+	[[Category: Barondeau, D P.]]
		+	[[Category: Fox, N G.]]
		+	[[Category: Hocker, B.]]
		+	[[Category: Malisi, C.]]
		+	[[Category: Meier, M M.]]
		+	[[Category: Rajendran, C.]]
		+	[[Category: Raushel, F M.]]
		+	[[Category: Schlee, S.]]
		+	[[Category: Sterner, R.]]
		+	[[Category: Xu, C.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Lactonase]]
		+	[[Category: Metalloenzyme]]
		+	[[Category: Nerve agent]]
		+	[[Category: Phosphotriesterase]]
		+	[[Category: Tim barrel]]

OCA: Protected "4j5n" [edit=sysop:move=sysop]

2013-02-20T13:31:05Z

Protected "4j5n" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:31, 20 February 2013

OCA: New page: '''Unreleased structure''' The entry 4j5n is ON HOLD Authors: Meier, M.M., Rajendran, C., Malisi, C., Fox, N.G., Xu, C., Schlee, S., Barondeau, D.P., Hocker, B., Sterner, R., Raushel, F...

2013-02-20T13:31:04Z

New page: '''Unreleased structure''' The entry 4j5n is ON HOLD Authors: Meier, M.M., Rajendran, C., Malisi, C., Fox, N.G., Xu, C., Schlee, S., Barondeau, D.P., Hocker, B., Sterner, R., Raushel, F...

New page

'''Unreleased structure'''

The entry 4j5n is ON HOLD

Authors: Meier, M.M., Rajendran, C., Malisi, C., Fox, N.G., Xu, C., Schlee, S., Barondeau, D.P., Hocker, B., Sterner, R., Raushel, F.M.

Description: Crystal Structure of a Deinococcus radiodurans PTE-like lactonase (drPLL) mutant Y28L/D71N/E101G/E179D/V235L/L270M