4k21 - Revision history

OCA at 12:07, 1 March 2024

2024-03-01T12:07:59Z

←Older revision		Revision as of 12:07, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k21]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K21 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k21]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K21 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k21 OCA], [https://pdbe.org/4k21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k21 RCSB], [https://www.ebi.ac.uk/pdbsum/4k21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k21 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k21 OCA], [https://pdbe.org/4k21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k21 RCSB], [https://www.ebi.ac.uk/pdbsum/4k21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k21 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/LECA_CANBL LECA_CANBL] D-mannose/D-glucose-binding lectin (PubMed:24865454). Has anti-inflammatory activity in animal models when applied intravenously (PubMed:24865454). Has antinociceptive activity in mice when applied intravenously (PubMed:19705102).<ref>PMID:19705102</ref> <ref>PMID:24865454</ref>		[https://www.uniprot.org/uniprot/LECA_CANBL LECA_CANBL] D-mannose/D-glucose-binding lectin (PubMed:24865454). Has anti-inflammatory activity in animal models when applied intravenously (PubMed:24865454). Has antinociceptive activity in mice when applied intravenously (PubMed:19705102).<ref>PMID:19705102</ref> <ref>PMID:24865454</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Plant lectins, especially those purified from species of the Leguminosae family, represent the best-studied group of carbohydrate-binding proteins. Lectins purified from seeds of the Diocleinae subtribe exhibit a high degree of sequence identity notwithstanding that they show very distinct biological activities. Two main factors have been related to this feature: variance in key residues influencing the carbohydrate-binding site geometry and differences in the pH-dependent oligomeric state profile. In this work, we have isolated a lectin from Canavalia boliviana (Cbol) and solved its x-ray crystal structure in the unbound form and in complex with the carbohydrates Man(alpha1-3)Man(alpha1-O)Me, Man(alpha1-4)Man(alpha1-O)Me and 5-bromo-4-chloro-3-indolyl-alpha-D-mannose. We evaluated its oligomerization profile at different pH values using Small Angle X-ray Scattering and compared it to that of Concanavalin A. Based on predicted pKa-shifts of amino acids in the subunit interfaces we devised a model for the dimer-tetramer equilibrium phenomena of these proteins. Additionally, we demonstrated Cbol anti-inflammatory properties and further characterized them using in vivo and in vitro models.
-
-	Structural Studies of an Anti-Inflammatory Lectin from Canavalia boliviana Seeds in Complex with Dimannosides.,Bezerra GA, Viertlmayr R, Moura TR, Delatorre P, Rocha BA, do Nascimento KS, Figueiredo JG, Bezerra IG, Teixeira CS, Simoes RC, Nagano CS, de Alencar NM, Gruber K, Cavada BS PLoS One. 2014 May 27;9(5):e97015. doi: 10.1371/journal.pone.0097015. eCollection, 2014. PMID:24865454<ref>PMID:24865454</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4k21" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:36, 30 November 2022

2022-11-30T11:36:58Z

←Older revision		Revision as of 11:36, 30 November 2022
Line 3:		Line 3:
	<StructureSection load='4k21' size='340' side='right'caption='[[4k21]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4k21' size='340' side='right'caption='[[4k21]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4k21]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K21 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4K21 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4k21]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K21 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k1y\|4k1y]], [[4k1z\|4k1z]], [[4k20\|4k20]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k21 OCA], [https://pdbe.org/4k21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k21 RCSB], [https://www.ebi.ac.uk/pdbsum/4k21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k21 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4k21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k21 OCA], [~~http~~://pdbe.org/4k21 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4k21 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4k21 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4k21 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LECA_CANBL LECA_CANBL] D-mannose/D-glucose-binding lectin (PubMed:24865454). Has anti-inflammatory activity in animal models when applied intravenously (PubMed:24865454). Has antinociceptive activity in mice when applied intravenously (PubMed:19705102).<ref>PMID:19705102</ref> <ref>PMID:24865454</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: Canavalia boliviana]]		[[Category: Canavalia boliviana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bezerra~~, G A~~]]	+	[[Category: Bezerra GA]]
-	[[Category: Cavada~~, B S~~]]	+	[[Category: Cavada BS]]
-	[[Category: Delatorre, P]]	+	[[Category: Delatorre P]]
-	[[Category: Gruber, K]]	+	[[Category: Gruber K]]
-	[[Category: Moura~~, T R~~]]	+	[[Category: Moura TR]]
-	[[Category: Nagano~~, C S~~]]	+	[[Category: Nagano CS]]
-	[[Category: Rocha~~, B A.M~~]]	+	[[Category: Rocha BAM]]
-	[[Category: Viertlmayr, R]]	+	[[Category: Viertlmayr R]]
-	~~[[Category: Binding site]]~~	+
-	~~[[Category: Carbohydrate binding]]~~	+
-	~~[[Category: Dimannoside]]~~	+
-	~~[[Category: Diocleinae lectin]]~~	+
-	~~[[Category: Jelly roll]]~~	+
-	~~[[Category: Mannose binding protein]]~~	+
-	~~[[Category: Oligomerization~~]]	+

OCA at 09:10, 3 April 2019

2019-04-03T09:10:07Z

←Older revision		Revision as of 09:10, 3 April 2019
Line 1:		Line 1:

	==Crystal structure of Canavalia boliviana lectin in complex with Xman==		==Crystal structure of Canavalia boliviana lectin in complex with Xman==
-	<StructureSection load='4k21' size='340' side='right' caption='[[4k21]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='4k21' size='340' side='right'caption='[[4k21]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k21]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K21 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k21]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K21 FirstGlance]. <br>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Canavalia boliviana]]		[[Category: Canavalia boliviana]]
		+	[[Category: Large Structures]]
	[[Category: Bezerra, G A]]		[[Category: Bezerra, G A]]
	[[Category: Cavada, B S]]		[[Category: Cavada, B S]]

OCA at 09:24, 11 August 2016

2016-08-11T09:24:21Z

←Older revision		Revision as of 09:24, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Canavalia boliviana lectin in complex with Xman==		==Crystal structure of Canavalia boliviana lectin in complex with Xman==
	<StructureSection load='4k21' size='340' side='right' caption='[[4k21]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4k21' size='340' side='right' caption='[[4k21]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k1y\|4k1y]], [[4k1z\|4k1z]], [[4k20\|4k20]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k1y\|4k1y]], [[4k1z\|4k1z]], [[4k20\|4k20]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k21 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k21 RCSB], [http://www.ebi.ac.uk/pdbsum/4k21 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k21 OCA], [http://pdbe.org/4k21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4k21 RCSB], [http://www.ebi.ac.uk/pdbsum/4k21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4k21 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4k21" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:53, 4 January 2015

2015-01-04T12:53:00Z

←Older revision		Revision as of 12:53, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4k21]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K21 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4k21]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K21 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k1y\|4k1y]], [[4k1z\|4k1z]], [[4k20\|4k20]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k1y\|4k1y]], [[4k1z\|4k1z]], [[4k20\|4k20]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k21 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k21 RCSB], [http://www.ebi.ac.uk/pdbsum/4k21 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k21 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k21 RCSB], [http://www.ebi.ac.uk/pdbsum/4k21 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Canavalia boliviana]]		[[Category: Canavalia boliviana]]
-	[[Category: Bezerra, G A.]]	+	[[Category: Bezerra, G A]]
-	[[Category: Cavada, B S.]]	+	[[Category: Cavada, B S]]
-	[[Category: Delatorre, P.]]	+	[[Category: Delatorre, P]]
-	[[Category: Gruber, K.]]	+	[[Category: Gruber, K]]
-	[[Category: Moura, T R.]]	+	[[Category: Moura, T R]]
-	[[Category: Nagano, C S.]]	+	[[Category: Nagano, C S]]
-	[[Category: Rocha, B A.M.]]	+	[[Category: Rocha, B A.M]]
-	[[Category: Viertlmayr, R.]]	+	[[Category: Viertlmayr, R]]
	[[Category: Binding site]]		[[Category: Binding site]]
	[[Category: Carbohydrate binding]]		[[Category: Carbohydrate binding]]

OCA at 07:18, 11 June 2014

2014-06-11T07:18:48Z

←Older revision		Revision as of 07:18, 11 June 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4k21\| PDB=4k21 \| SCENE= }}~~	+	==Crystal structure of Canavalia boliviana lectin in complex with Xman==
-	===Crystal structure of Canavalia boliviana lectin in complex with Xman===	+	<StructureSection load='4k21' size='340' side='right' caption='[[4k21]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4k21]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K21 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4k1y\|4k1y]], [[4k1z\|4k1z]], [[4k20\|4k20]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k21 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k21 RCSB], [http://www.ebi.ac.uk/pdbsum/4k21 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Plant lectins, especially those purified from species of the Leguminosae family, represent the best-studied group of carbohydrate-binding proteins. Lectins purified from seeds of the Diocleinae subtribe exhibit a high degree of sequence identity notwithstanding that they show very distinct biological activities. Two main factors have been related to this feature: variance in key residues influencing the carbohydrate-binding site geometry and differences in the pH-dependent oligomeric state profile. In this work, we have isolated a lectin from Canavalia boliviana (Cbol) and solved its x-ray crystal structure in the unbound form and in complex with the carbohydrates Man(alpha1-3)Man(alpha1-O)Me, Man(alpha1-4)Man(alpha1-O)Me and 5-bromo-4-chloro-3-indolyl-alpha-D-mannose. We evaluated its oligomerization profile at different pH values using Small Angle X-ray Scattering and compared it to that of Concanavalin A. Based on predicted pKa-shifts of amino acids in the subunit interfaces we devised a model for the dimer-tetramer equilibrium phenomena of these proteins. Additionally, we demonstrated Cbol anti-inflammatory properties and further characterized them using in vivo and in vitro models.

-	~~==About this Structure==~~	+	Structural Studies of an Anti-Inflammatory Lectin from Canavalia boliviana Seeds in Complex with Dimannosides.,Bezerra GA, Viertlmayr R, Moura TR, Delatorre P, Rocha BA, do Nascimento KS, Figueiredo JG, Bezerra IG, Teixeira CS, Simoes RC, Nagano CS, de Alencar NM, Gruber K, Cavada BS PLoS One. 2014 May 27;9(5):e97015. doi: 10.1371/journal.pone.0097015. eCollection, 2014. PMID:24865454<ref>PMID:24865454</ref>
-	~~[[4k21]] is a 1 chain structure with sequence~~ from ~~[http~~:~~//en~~.~~wikipedia~~.~~org/wiki~~/~~Canavalia_boliviana Canavalia boliviana]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id~~=~~4K21 OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Canavalia boliviana]]		[[Category: Canavalia boliviana]]
	[[Category: Bezerra, G A.]]		[[Category: Bezerra, G A.]]

OCA at 06:42, 9 April 2014

2014-04-09T06:42:09Z

←Older revision		Revision as of 06:42, 9 April 2014
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-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4k21\| PDB=4k21 \| SCENE= }}
		+	===Crystal structure of Canavalia boliviana lectin in complex with Xman===

-	~~The entry~~ 4k21 is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4k21]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_boliviana Canavalia boliviana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K21 OCA].
-	~~Authors~~: Bezerra, G.A., ~~Moura, T~~.~~R., Viertlmayr, R.,~~ Delatorre, P., ~~Rocha, B.A.M~~., Nagano, C.S.~~, Gruber, K., Cavada~~, B.S.	+	[[Category: Canavalia boliviana]]
-		+	[[Category: Bezerra, G A.]]
-	~~Description~~: ~~Crystal structure of Canavalia boliviana~~ lectin ~~in complex with Xman~~	+	[[Category: Cavada, B S.]]
		+	[[Category: Delatorre, P.]]
		+	[[Category: Gruber, K.]]
		+	[[Category: Moura, T R.]]
		+	[[Category: Nagano, C S.]]
		+	[[Category: Rocha, B A.M.]]
		+	[[Category: Viertlmayr, R.]]
		+	[[Category: Binding site]]
		+	[[Category: Carbohydrate binding]]
		+	[[Category: Dimannoside]]
		+	[[Category: Diocleinae lectin]]
		+	[[Category: Jelly roll]]
		+	[[Category: Mannose binding protein]]
		+	[[Category: Oligomerization]]

OCA at 08:46, 30 June 2013

2013-06-30T08:46:25Z

←Older revision		Revision as of 08:46, 30 June 2013
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4k21 is ON HOLD	+	The entry 4k21 is ON HOLD until Paper Publication

	Authors: Bezerra, G.A., Moura, T.R., Viertlmayr, R., Delatorre, P., Rocha, B.A.M., Nagano, C.S., Gruber, K., Cavada, B.S.		Authors: Bezerra, G.A., Moura, T.R., Viertlmayr, R., Delatorre, P., Rocha, B.A.M., Nagano, C.S., Gruber, K., Cavada, B.S.

	Description: Crystal structure of Canavalia boliviana lectin in complex with Xman		Description: Crystal structure of Canavalia boliviana lectin in complex with Xman

OCA: Protected "4k21" [edit=sysop:move=sysop]

2013-04-17T20:06:19Z

Protected "4k21" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:06, 17 April 2013

OCA: New page: '''Unreleased structure''' The entry 4k21 is ON HOLD Authors: Bezerra, G.A., Moura, T.R., Viertlmayr, R., Delatorre, P., Rocha, B.A.M., Nagano, C.S., Gruber, K., Cavada, B.S. Descripti...

2013-04-17T20:06:18Z

New page: '''Unreleased structure''' The entry 4k21 is ON HOLD Authors: Bezerra, G.A., Moura, T.R., Viertlmayr, R., Delatorre, P., Rocha, B.A.M., Nagano, C.S., Gruber, K., Cavada, B.S. Descripti...

New page

'''Unreleased structure'''

The entry 4k21 is ON HOLD

Authors: Bezerra, G.A., Moura, T.R., Viertlmayr, R., Delatorre, P., Rocha, B.A.M., Nagano, C.S., Gruber, K., Cavada, B.S.

Description: Crystal structure of Canavalia boliviana lectin in complex with Xman