4kic - Revision history

OCA at 14:30, 8 November 2023

2023-11-08T14:30:18Z

←Older revision		Revision as of 14:30, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4kic]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_hygroscopicus Streptomyces hygroscopicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KIC FirstGlance]. <br>		<table><tr><td colspan='2'>[[4kic]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_hygroscopicus Streptomyces hygroscopicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KIC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.43Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [https://pdbe.org/4kic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [https://www.ebi.ac.uk/pdbsum/4kic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kic ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [https://pdbe.org/4kic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [https://www.ebi.ac.uk/pdbsum/4kic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kic ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:53, 7 December 2022

2022-12-07T08:53:46Z

←Older revision		Revision as of 08:53, 7 December 2022
Line 3:		Line 3:
	<StructureSection load='4kic' size='340' side='right'caption='[[4kic]], [[Resolution\|resolution]] 2.43Å' scene=''>		<StructureSection load='4kic' size='340' side='right'caption='[[4kic]], [[Resolution\|resolution]] 2.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kic]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"actinomyces_hygroscopicus"_jensen_1931 "actinomyces~~ hygroscopicus~~" jensen 1931~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4KIC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4kic]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_hygroscopicus Streptomyces hygroscopicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KIC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kib\|4kib]], [[4kif\|4kif]], [[4kig\|4kig]], [[4m6x\|4m6x]], [[4m6y\|4m6y]], [[4m71\|4m71]], [[4m72\|4m72]], [[4m73\|4m73]], [[4m74\|4m74]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [https://pdbe.org/4kic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [https://www.ebi.ac.uk/pdbsum/4kic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kic ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mppJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1912 "Actinomyces hygroscopicus" Jensen 1931])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [~~http~~://pdbe.org/4kic PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4kic PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4kic ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MPPJ_STRHY MPPJ_STRHY]] S-adenosyl-L-methionine-dependent methyltransferase involved in synthesis of the nonproteinogenic amino acid (2S,3S)-beta-methyl-phenylalanine, a building block of the antibiotic mannopeptimycin.	+	[https://www.uniprot.org/uniprot/MPPJ_STRHY MPPJ_STRHY] S-adenosyl-L-methionine-dependent methyltransferase involved in synthesis of the nonproteinogenic amino acid (2S,3S)-beta-methyl-phenylalanine, a building block of the antibiotic mannopeptimycin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
-	*[[SAM-dependent ~~methyltransferase~~\|SAM-dependent ~~methyltransferase~~]]	+	*[[SAM-dependent methyltrasferase 3D structures\|SAM-dependent methyltrasferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Actinomyces hygroscopicus jensen 1931]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chan, H C~~]]	+	[[Category: Streptomyces hygroscopicus]]
-	[[Category: ~~Huang, C J~~]]	+	[[Category: Chan HC]]
-	[[Category: ~~Li, T L~~]]	+	[[Category: Huang CJ]]
-	[[Category: ~~Liu, Y C~~]]	+	[[Category: Li TL]]
-	[[Category: ~~Zou, X W~~]]	+	[[Category: Liu YC]]
-	[[Category: ~~Methyltransferase]]~~	+	[[Category: Zou XW]]
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Sam binding ppy binding]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:32, 3 April 2019

2019-04-03T09:32:48Z

←Older revision		Revision as of 09:32, 3 April 2019
Line 1:		Line 1:

	==Crystal structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-methionine and phenylpyruvic acid==		==Crystal structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-methionine and phenylpyruvic acid==
-	<StructureSection load='4kic' size='340' side='right' caption='[[4kic]], [[Resolution\|resolution]] 2.43Å' scene=''>	+	<StructureSection load='4kic' size='340' side='right'caption='[[4kic]], [[Resolution\|resolution]] 2.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kic]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KIC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4kic]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_hygroscopicus"_jensen_1931 "actinomyces hygroscopicus" jensen 1931]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KIC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kib\|4kib]], [[4kif\|4kif]], [[4kig\|4kig]], [[4m6x\|4m6x]], [[4m6y\|4m6y]], [[4m71\|4m71]], [[4m72\|4m72]], [[4m73\|4m73]], [[4m74\|4m74]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kib\|4kib]], [[4kif\|4kif]], [[4kig\|4kig]], [[4m6x\|4m6x]], [[4m6y\|4m6y]], [[4m71\|4m71]], [[4m72\|4m72]], [[4m73\|4m73]], [[4m74\|4m74]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mppJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1912 "Actinomyces hygroscopicus" Jensen 1931])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [http://pdbe.org/4kic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [http://www.ebi.ac.uk/pdbsum/4kic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kic ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [http://pdbe.org/4kic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [http://www.ebi.ac.uk/pdbsum/4kic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kic ProSAT]</span></td></tr>
	</table>		</table>
Line 19:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4kic" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4kic" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[SAM-dependent methyltransferase\|SAM-dependent methyltransferase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Actinomyces hygroscopicus jensen 1931]]
		+	[[Category: Large Structures]]
	[[Category: Chan, H C]]		[[Category: Chan, H C]]
	[[Category: Huang, C J]]		[[Category: Huang, C J]]

OCA at 15:22, 4 August 2016

2016-08-04T15:22:02Z

←Older revision		Revision as of 15:22, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-methionine and phenylpyruvic acid==		==Crystal structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-methionine and phenylpyruvic acid==
	<StructureSection load='4kic' size='340' side='right' caption='[[4kic]], [[Resolution\|resolution]] 2.43Å' scene=''>		<StructureSection load='4kic' size='340' side='right' caption='[[4kic]], [[Resolution\|resolution]] 2.43Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kib\|4kib]], [[4kif\|4kif]], [[4kig\|4kig]], [[4m6x\|4m6x]], [[4m6y\|4m6y]], [[4m71\|4m71]], [[4m72\|4m72]], [[4m73\|4m73]], [[4m74\|4m74]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kib\|4kib]], [[4kif\|4kif]], [[4kig\|4kig]], [[4m6x\|4m6x]], [[4m6y\|4m6y]], [[4m71\|4m71]], [[4m72\|4m72]], [[4m73\|4m73]], [[4m74\|4m74]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [http://www.ebi.ac.uk/pdbsum/4kic PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [http://pdbe.org/4kic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [http://www.ebi.ac.uk/pdbsum/4kic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kic ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4kic" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:21, 24 December 2014

2014-12-24T16:21:26Z

←Older revision		Revision as of 16:21, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4kic]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KIC FirstGlance]. <br>		<table><tr><td colspan='2'>[[4kic]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KIC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kib\|4kib]], [[4kif\|4kif]], [[4kig\|4kig]], [[4m6x\|4m6x]], [[4m6y\|4m6y]], [[4m71\|4m71]], [[4m72\|4m72]], [[4m73\|4m73]], [[4m74\|4m74]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kib\|4kib]], [[4kif\|4kif]], [[4kig\|4kig]], [[4m6x\|4m6x]], [[4m6y\|4m6y]], [[4m71\|4m71]], [[4m72\|4m72]], [[4m73\|4m73]], [[4m74\|4m74]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [http://www.ebi.ac.uk/pdbsum/4kic PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [http://www.ebi.ac.uk/pdbsum/4kic PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MPPJ_STRHY MPPJ_STRHY]] S-adenosyl-L-methionine-dependent methyltransferase involved in synthesis of the nonproteinogenic amino acid (2S,3S)-beta-methyl-phenylalanine, a building block of the antibiotic mannopeptimycin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chan, H C.]]	+	[[Category: Chan, H C]]
-	[[Category: Huang, C J.]]	+	[[Category: Huang, C J]]
-	[[Category: Li, T L.]]	+	[[Category: Li, T L]]
-	[[Category: Liu, Y C.]]	+	[[Category: Liu, Y C]]
-	[[Category: Zou, X W.]]	+	[[Category: Zou, X W]]
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
	[[Category: Sam binding ppy binding]]		[[Category: Sam binding ppy binding]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 05:11, 25 June 2014

2014-06-25T05:11:29Z

←Older revision		Revision as of 05:11, 25 June 2014
Line 2:		Line 2:
	<StructureSection load='4kic' size='340' side='right' caption='[[4kic]], [[Resolution\|resolution]] 2.43Å' scene=''>		<StructureSection load='4kic' size='340' side='right' caption='[[4kic]], [[Resolution\|resolution]] 2.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kic]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIC OCA]. <br>	+	<table><tr><td colspan='2'>[[4kic]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KIC FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene><br>		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene><br>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kib\|4kib]], [[4kif\|4kif]], [[4kig\|4kig]]~~</td></tr>~~	+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kib\|4kib]], [[4kif\|4kif]], [[4kig\|4kig]], [[4m6x\|4m6x]], [[4m6y\|4m6y]], [[4m71\|4m71]], [[4m72\|4m72]], [[4m73\|4m73]], [[4m74\|4m74]]</td></tr>
-	~~<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>~~[~~http://en.wikipedia.org/wiki/Glucokinase Glucokinase~~], ~~with EC number~~ [~~http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2~~] ~~</span>~~</td></tr>	+
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [http://www.ebi.ac.uk/pdbsum/4kic PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [http://www.ebi.ac.uk/pdbsum/4kic PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In biological systems, methylation is most commonly performed by methyltransferases (MTs) using the electrophilic methyl source S-adenosyl-L-methionine (SAM) via the SN2 mechanism. (2S,3S)-beta-Methylphenylalanine, a nonproteinogenic amino acid, is a building unit of the glycopeptide antibiotic mannopeptimycin. The gene product of mppJ from the mannopeptimycin-biosynthetic gene cluster is the MT that methylates the benzylic C atom of phenylpyruvate (Ppy) to give betaMePpy. Although the benzylic C atom of Ppy is acidic, how its nucleophilicity is further enhanced to become an acceptor for C-methylation has not conclusively been determined. Here, a structural approach is used to address the mechanism of MppJ and to engineer it for new functions. The purified MppJ displays a turquoise colour, implying the presence of a metal ion. The crystal structures reveal MppJ to be the first ferric ion SAM-dependent MT. An additional four structures of binary and ternary complexes illustrate the molecular mechanism for the metal ion-dependent methyltransfer reaction. Overall, MppJ has a nonhaem iron centre that bind, orients and activates the alpha-ketoacid substrate and has developed a sandwiched bi-water device to avoid the formation of the unwanted reactive oxo-iron(IV) species during the C-methylation reaction. This discovery further prompted the conversion of the MT into a structurally/functionally unrelated new enzyme. Through stepwise mutagenesis and manipulation of coordination chemistry, MppJ was engineered to perform both Lewis acid-assisted hydration and/or O-methyltransfer reactions to give stereospecific new compounds. This process was validated by six crystal structures. The results reported in this study will facilitate the development and design of new biocatalysts for difficult-to-synthesize biochemicals.
		+
		+	Structure and mechanism of a nonhaem-iron SAM-dependent C-methyltransferase and its engineering to a hydratase and an O-methyltransferase.,Zou XW, Liu YC, Hsu NS, Huang CJ, Lyu SY, Chan HC, Chang CY, Yeh HW, Lin KH, Wu CJ, Tsai MD, Li TL Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1549-60. doi:, 10.1107/S1399004714005239. Epub 2014 May 23. PMID:24914966<ref>PMID:24914966</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:19, 7 May 2014

2014-05-07T08:19:48Z

←Older revision		Revision as of 08:19, 7 May 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-methionine and phenylpyruvic acid==
-		+	<StructureSection load='4kic' size='340' side='right' caption='[[4kic]], [[Resolution\|resolution]] 2.43Å' scene=''>
-	~~The entry~~ 4kic is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4kic]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KIC OCA]. <br>
-	~~Authors~~: ~~Liu~~, Y.C., ~~Zou~~, X.W., Chan, H.C., Huang, C.J, Li, T.L.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HF2:(2R)-2-HYDROXY-3-PHENYLPROPANOIC+ACID'>HF2</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=PPY:3-PHENYLPYRUVIC+ACID'>PPY</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene><br>
-		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kib\|4kib]], [[4kif\|4kif]], [[4kig\|4kig]]</td></tr>
-	~~Description~~: ~~Crystal structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-methionine and phenylpyruvic acid~~	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kic RCSB], [http://www.ebi.ac.uk/pdbsum/4kic PDBsum]</span></td></tr>
		+	<table>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chan, H C.]]
		+	[[Category: Huang, C J.]]
		+	[[Category: Li, T L.]]
		+	[[Category: Liu, Y C.]]
		+	[[Category: Zou, X W.]]
		+	[[Category: Methyltransferase]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Sam binding ppy binding]]
		+	[[Category: Transferase]]

OCA at 07:45, 21 August 2013

2013-08-21T07:45:53Z

←Older revision		Revision as of 07:45, 21 August 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4kic is ON HOLD	+	The entry 4kic is ON HOLD until Paper Publication

	Authors: Liu, Y.C., Zou, X.W., Chan, H.C., Huang, C.J, Li, T.L.		Authors: Liu, Y.C., Zou, X.W., Chan, H.C., Huang, C.J, Li, T.L.

	Description: Crystal structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-methionine and phenylpyruvic acid		Description: Crystal structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-methionine and phenylpyruvic acid

OCA: Protected "4kic" [edit=sysop:move=sysop]

2013-05-08T07:24:41Z

Protected "4kic" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:24, 8 May 2013

OCA: New page: '''Unreleased structure''' The entry 4kic is ON HOLD Authors: Liu, Y.C., Zou, X.W., Chan, H.C., Huang, C.J, Li, T.L. Description: Crystal structure of methyltransferase from Streptomyc...

2013-05-08T07:24:40Z

New page: '''Unreleased structure''' The entry 4kic is ON HOLD Authors: Liu, Y.C., Zou, X.W., Chan, H.C., Huang, C.J, Li, T.L. Description: Crystal structure of methyltransferase from Streptomyc...

New page

'''Unreleased structure'''

The entry 4kic is ON HOLD

Authors: Liu, Y.C., Zou, X.W., Chan, H.C., Huang, C.J, Li, T.L.

Description: Crystal structure of methyltransferase from Streptomyces hygroscopicus complexed with S-adenosyl-L-methionine and phenylpyruvic acid