4kpg - Revision history

OCA at 09:05, 7 December 2022

2022-12-07T09:05:07Z

←Older revision		Revision as of 09:05, 7 December 2022
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	==Crystal structure of MycP1 from the ESX-1 type VII secretion system==		==Crystal structure of MycP1 from the ESX-1 type VII secretion system==
-	<StructureSection load='4kpg' size='340' side='right' caption='[[4kpg]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='4kpg' size='340' side='right'caption='[[4kpg]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kpg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Mycs2 Mycs2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4KPG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4kpg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KPG FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4j94\|4j94]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpg OCA], [https://pdbe.org/4kpg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kpg RCSB], [https://www.ebi.ac.uk/pdbsum/4kpg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kpg ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MSMEG_0083, MSMEI_0081 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4kpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpg OCA], [~~http~~://pdbe.org/4kpg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4kpg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4kpg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4kpg ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MYCP1_MYCS2 MYCP1_MYCS2] May play a dual role in regulation of ESX-1 secretion and virulence. Acts as a protease that cleaves EspB.<ref>PMID:20227664</ref> <ref>PMID:23620593</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mycs2~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Subtilisin~~]]	+	[[Category: Mycolicibacterium smegmatis MC2 155]]
-	[[Category: Gruninger~~, R J~~]]	+	[[Category: Gruninger RJ]]
-	[[Category: Prehna, G]]	+	[[Category: Prehna G]]
-	[[Category: Solomonson, M]]	+	[[Category: Solomonson M]]
-	[[Category: Strynadka~~, N C.J~~]]	+	[[Category: Strynadka NCJ]]
-	[[Category: Wasney~~, G A~~]]	+	[[Category: Wasney GA]]
-	[[Category: Watanabe, N]]	+	[[Category: Watanabe N]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protease~~]]	+

OCA at 02:11, 5 August 2016

2016-08-05T02:11:29Z

←Older revision		Revision as of 02:11, 5 August 2016
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		+
	==Crystal structure of MycP1 from the ESX-1 type VII secretion system==		==Crystal structure of MycP1 from the ESX-1 type VII secretion system==
	<StructureSection load='4kpg' size='340' side='right' caption='[[4kpg]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4kpg' size='340' side='right' caption='[[4kpg]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KPG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4kpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KPG FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4j94\|4j94]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4j94\|4j94]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MSMEG_0083, MSMEI_0081 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 ~~Mycobacterium smegmatis str. MC2 155~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MSMEG_0083, MSMEI_0081 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kpg RCSB], [http://www.ebi.ac.uk/pdbsum/4kpg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpg OCA], [http://pdbe.org/4kpg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kpg RCSB], [http://www.ebi.ac.uk/pdbsum/4kpg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kpg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4kpg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mycobacterium smegmatis str. mc2 155~~]]	+	[[Category: Mycs2]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
	[[Category: Gruninger, R J]]		[[Category: Gruninger, R J]]

OCA at 15:31, 21 December 2014

2014-12-21T15:31:29Z

←Older revision		Revision as of 15:31, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4kpg\| PDB=4kpg \| SCENE= }}~~	+	==Crystal structure of MycP1 from the ESX-1 type VII secretion system==
-	===Crystal structure of MycP1 from the ESX-1 type VII secretion system===	+	<StructureSection load='4kpg' size='340' side='right' caption='[[4kpg]], [[Resolution\|resolution]] 2.15Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23620593}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4kpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KPG FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4j94\|4j94]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MSMEG_0083, MSMEI_0081 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 Mycobacterium smegmatis str. MC2 155])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kpg RCSB], [http://www.ebi.ac.uk/pdbsum/4kpg PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mycobacteria use specialized type VII (ESX) secretion systems to export proteins across their complex cell walls. Mycobacterium tuberculosis encodes five nonredundant ESX secretion systems, with ESX-1 being particularly important to disease progression. All ESX loci encode extracellular membrane-bound proteases called mycosins (MycP) that are essential to secretion and have been shown to be involved in processing of type VII-exported proteins. Here, we report the first x-ray crystallographic structure of MycP1(24-407) to 1.86 A, defining a subtilisin-like fold with a unique N-terminal extension previously proposed to function as a propeptide for regulation of enzyme activity. The structure reveals that this N-terminal extension shows no structural similarity to previously characterized protease propeptides and instead wraps intimately around the catalytic domain where, tethered by a disulfide bond, it forms additional interactions with a unique extended loop that protrudes from the catalytic core. We also show MycP1 cleaves the ESX-1 secreted protein EspB from both M. tuberculosis and Mycobacterium smegmatis at a homologous cut site in vitro.

-	~~==About this~~ Structure==	+	Structure of the Mycosin-1 Protease from the Mycobacterial ESX-1 Protein Type VII Secretion System.,Solomonson M, Huesgen PF, Wasney GA, Watanabe N, Gruninger RJ, Prehna G, Overall CM, Strynadka NC J Biol Chem. 2013 Jun 14;288(24):17782-90. doi: 10.1074/jbc.M113.462036. Epub, 2013 Apr 25. PMID:23620593<ref>PMID:23620593</ref>
-	~~[[4kpg]] is a~~ 1 ~~chain structure with sequence~~ from ~~[http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Mycobacterium_smegmatis_str~~.~~_mc2_155 Mycobacterium smegmatis str~~. ~~mc2 155]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4KPG OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023620593</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Mycobacterium smegmatis str. mc2 155]]		[[Category: Mycobacterium smegmatis str. mc2 155]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
-	[[Category: Gruninger, R J.]]	+	[[Category: Gruninger, R J]]
-	[[Category: Prehna, G.]]	+	[[Category: Prehna, G]]
-	[[Category: Solomonson, M.]]	+	[[Category: Solomonson, M]]
-	[[Category: Strynadka, N C.J.]]	+	[[Category: Strynadka, N C.J]]
-	[[Category: Wasney, G A.]]	+	[[Category: Wasney, G A]]
-	[[Category: Watanabe, N.]]	+	[[Category: Watanabe, N]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Protease]]		[[Category: Protease]]
-	[[Category: Subtilisin]]

OCA at 14:29, 3 July 2013

2013-07-03T14:29:37Z

←Older revision		Revision as of 14:29, 3 July 2013
Line 4:		Line 4:

	==About this Structure==		==About this Structure==
-	[[4kpg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPG OCA].	+	[[4kpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPG OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:023620593</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:023620593</ref><references group="xtra"/><references/>
		+	[[Category: Mycobacterium smegmatis str. mc2 155]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
	[[Category: Gruninger, R J.]]		[[Category: Gruninger, R J.]]

OCA at 11:54, 3 July 2013

2013-07-03T11:54:31Z

←Older revision		Revision as of 11:54, 3 July 2013
Line 4:		Line 4:

	==About this Structure==		==About this Structure==
-	[[4kpg]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPG OCA].	+	[[4kpg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPG OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:023620593</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:023620593</ref><references group="xtra"/><references/>
-	[[Category: Mycobacterium smegmatis str. mc2 155]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
	[[Category: Gruninger, R J.]]		[[Category: Gruninger, R J.]]

OCA: Protected "4kpg" [edit=sysop:move=sysop]

2013-06-19T09:08:03Z

Protected "4kpg" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:08, 19 June 2013

OCA: New page: {{STRUCTURE_4kpg| PDB=4kpg | SCENE= }} ===Crystal structure of MycP1 from the ESX-1 type VII secretion system=== {{ABSTRACT_PUBMED_23620593}} ==About this Structure== 4kpg is a 1...

2013-06-19T09:08:01Z

New page: {{STRUCTURE_4kpg| PDB=4kpg | SCENE= }} ===Crystal structure of MycP1 from the ESX-1 type VII secretion system=== {{ABSTRACT_PUBMED_23620593}} ==About this Structure== 4kpg is a 1...

New page

{{STRUCTURE_4kpg| PDB=4kpg | SCENE= }}
===Crystal structure of MycP1 from the ESX-1 type VII secretion system===
{{ABSTRACT_PUBMED_23620593}}

==About this Structure==
[[4kpg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPG OCA].

==Reference==
<ref group="xtra">PMID:023620593</ref><references group="xtra"/><references/>
[[Category: Mycobacterium smegmatis str. mc2 155]]
[[Category: Subtilisin]]
[[Category: Gruninger, R J.]]
[[Category: Prehna, G.]]
[[Category: Solomonson, M.]]
[[Category: Strynadka, N C.J.]]
[[Category: Wasney, G A.]]
[[Category: Watanabe, N.]]
[[Category: Hydrolase]]
[[Category: Protease]]
[[Category: Subtilisin]]