4kpo - Revision history

OCA at 16:03, 20 September 2023

2023-09-20T16:03:27Z

←Older revision		Revision as of 16:03, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4kpo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KPO FirstGlance]. <br>		<table><tr><td colspan='2'>[[4kpo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KPO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.49Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpo OCA], [https://pdbe.org/4kpo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kpo RCSB], [https://www.ebi.ac.uk/pdbsum/4kpo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kpo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpo OCA], [https://pdbe.org/4kpo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kpo RCSB], [https://www.ebi.ac.uk/pdbsum/4kpo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kpo ProSAT]</span></td></tr>
	</table>		</table>

OCA at 09:05, 7 December 2022

2022-12-07T09:05:40Z

←Older revision		Revision as of 09:05, 7 December 2022
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	==Plant nucleoside hydrolase - ZmNRh3 enzyme==		==Plant nucleoside hydrolase - ZmNRh3 enzyme==
-	<StructureSection load='4kpo' size='340' side='right' caption='[[4kpo]], [[Resolution\|resolution]] 2.49Å' scene=''>	+	<StructureSection load='4kpo' size='340' side='right'caption='[[4kpo]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4kpo]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Maize Maize~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4KPO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4kpo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KPO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kpn\|4kpn]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpo OCA], [https://pdbe.org/4kpo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kpo RCSB], [https://www.ebi.ac.uk/pdbsum/4kpo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kpo ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NRH3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4kpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpo OCA], [~~http~~://pdbe.org/4kpo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4kpo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4kpo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4kpo ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B6T563_MAIZE B6T563_MAIZE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Maize~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Kopecny, D~~]]	+	[[Category: Zea mays]]
-	[[Category: ~~Morera, S~~]]	+	[[Category: Kopecny D]]
-	[[Category: ~~Vigouroux, A~~]]	+	[[Category: Morera S]]
-	[[Category: ~~Hydrolase]]~~	+	[[Category: Vigouroux A]]
-	~~[[Category: Iu-nrh~~]]	+

OCA at 17:55, 11 August 2016

2016-08-11T17:55:15Z

←Older revision		Revision as of 17:55, 11 August 2016
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		+
	==Plant nucleoside hydrolase - ZmNRh3 enzyme==		==Plant nucleoside hydrolase - ZmNRh3 enzyme==
	<StructureSection load='4kpo' size='340' side='right' caption='[[4kpo]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='4kpo' size='340' side='right' caption='[[4kpo]], [[Resolution\|resolution]] 2.49Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kpn\|4kpn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kpn\|4kpn]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NRH3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NRH3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kpo RCSB], [http://www.ebi.ac.uk/pdbsum/4kpo PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpo OCA], [http://pdbe.org/4kpo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kpo RCSB], [http://www.ebi.ac.uk/pdbsum/4kpo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kpo ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4kpo" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:38, 4 January 2015

2015-01-04T12:38:52Z

←Older revision		Revision as of 12:38, 4 January 2015
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-	~~{{STRUCTURE_4kpo~~\| ~~PDB~~=4kpo \| ~~SCENE~~= }}	+	==Plant nucleoside hydrolase - ZmNRh3 enzyme==
-	===~~Plant nucleoside hydrolase~~ - ~~ZmNRh3 enzyme~~===	+	<StructureSection load='4kpo' size='340' side='right' caption='[[4kpo]], [[Resolution\|resolution]] 2.49Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24170203}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4kpo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KPO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kpn\|4kpn]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NRH3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kpo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kpo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kpo RCSB], [http://www.ebi.ac.uk/pdbsum/4kpo PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We present a comprehensive characterization of the nucleoside N-ribohydrolase (NRH) family in two model plants, Physcomitrella patens (PpNRH) and Zea mays (ZmNRH), using "in vitro" and "in planta" approaches. We identified two NRH subclasses in the plant kingdom; one preferentially targets the purine ribosides inosine and xanthosine while the other is more active towards uridine and xanthosine. Both subclasses can hydrolyze plant hormones - cytokinin ribosides. We also solved the crystal structures of two purine NRHs, PpNRH1 and ZmNRH3. Structural analyses, site-directed mutagenesis experiments, and phylogenetic studies were conducted to identify the residues responsible for the observed differences in substrate specificity between the NRH isoforms. The presence of a tyrosine at position 249 (PpNRH1 numbering) confers high hydrolase activity for purine ribosides, while an aspartate residue in this position confers high activity for uridine. Bud formation is delayed by knocking out single NRH genes in Physcomitrella, and under conditions of nitrogen shortage, PpNRH1-deficient plants cannot salvage adenosine-bound nitrogen. All PpNRH knockout plants display elevated levels of certain purine and pyrimidine ribosides and cytokinins that reflect the substrate preferences of the knocked-out enzymes. NRH enzymes thus have functions in cytokinin conversion and activation as well as in purine and pyrimidine metabolism.

-	~~==About this~~ Structure==	+	Structure and function of nucleoside hydrolases from Physcomitrella and maize catalyzing hydrolysis of purine, pyrimidine and cytokinin ribosides.,Kopecna M, Blaschke H, Kopecny D, Vigouroux A, Koncitikova R, Novak O, Kotland O, Strnad M, Morera S, von Schwartzenberg K Plant Physiol. 2013 Oct 29. PMID:24170203<ref>PMID:24170203</ref>
-	~~[[4kpo]] is a 2 chain structure with sequence~~ from ~~[http://en~~.~~wikipedia~~.~~org/wiki/Maize Maize]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4KPO OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024170203</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Maize]]		[[Category: Maize]]
-	[[Category: Kopecny, D.]]	+	[[Category: Kopecny, D]]
-	[[Category: Morera, S.]]	+	[[Category: Morera, S]]
-	[[Category: Vigouroux, A.]]	+	[[Category: Vigouroux, A]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Iu-nrh]]		[[Category: Iu-nrh]]

OCA at 08:12, 18 December 2013

2013-12-18T08:12:27Z

←Older revision		Revision as of 08:12, 18 December 2013
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	==About this Structure==		==About this Structure==
-	[[4kpo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPO OCA].	+	[[4kpo]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPO OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:024170203</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:024170203</ref><references group="xtra"/><references/>
		+	[[Category: Maize]]
	[[Category: Kopecny, D.]]		[[Category: Kopecny, D.]]
	[[Category: Morera, S.]]		[[Category: Morera, S.]]

OCA at 10:20, 27 November 2013

2013-11-27T10:20:48Z

←Older revision		Revision as of 10:20, 27 November 2013
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-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4kpo\| PDB=4kpo \| SCENE= }}
		+	===Plant nucleoside hydrolase - ZmNRh3 enzyme===
		+	{{ABSTRACT_PUBMED_24170203}}

-	~~The entry~~ 4kpo is ~~ON HOLD~~	+	==About this Structure==
		+	[[4kpo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KPO OCA].

-	~~Authors~~: Morera, S., Vigouroux, A.~~, Kopecny, D.~~	+	==Reference==
-		+	<ref group="xtra">PMID:024170203</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Plant nucleoside hydrolase~~	+	[[Category: Kopecny, D.]]
		+	[[Category: Morera, S.]]
		+	[[Category: Vigouroux, A.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Iu-nrh]]

OCA at 05:50, 23 October 2013

2013-10-23T05:50:49Z

←Older revision		Revision as of 05:50, 23 October 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4kpo is ON HOLD ~~until Paper Publication~~	+	The entry 4kpo is ON HOLD

	Authors: Morera, S., Vigouroux, A., Kopecny, D.		Authors: Morera, S., Vigouroux, A., Kopecny, D.

	Description: Plant nucleoside hydrolase		Description: Plant nucleoside hydrolase

OCA: Protected "4kpo" [edit=sysop:move=sysop]

2013-06-19T11:52:44Z

Protected "4kpo" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:52, 19 June 2013

OCA: New page: '''Unreleased structure''' The entry 4kpo is ON HOLD until Paper Publication Authors: Morera, S., Vigouroux, A., Kopecny, D. Description: Plant nucleoside hydrolase

2013-06-19T11:52:42Z

New page: '''Unreleased structure''' The entry 4kpo is ON HOLD until Paper Publication Authors: Morera, S., Vigouroux, A., Kopecny, D. Description: Plant nucleoside hydrolase

New page

'''Unreleased structure'''

The entry 4kpo is ON HOLD until Paper Publication

Authors: Morera, S., Vigouroux, A., Kopecny, D.

Description: Plant nucleoside hydrolase