4kx2 - Revision history

OCA: Redirecting to 1vvj

2014-12-10T15:48:46Z

Redirecting to 1vvj

←Older revision		Revision as of 15:48, 10 December 2014
Line 1:		Line 1:
-	~~==Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome==~~	+	#REDIRECT [[1vvj]] This PDB entry is obsolete and replaced by 1vvj
-	~~<StructureSection load='4kx2' size='340' side='right' caption='~~[[~~4kx2]], [[Resolution\|resolution]] 3.44Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[4kx2~~]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KX2 FirstGlance]. <br>	+
-	~~</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>~~	+
-	~~<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPU:PUROMYCIN-5-MONOPHOSPHATE'>PPU</scene></td></tr>~~	+
-	~~<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kwz\|4kwz]], [[4kx0\|4kx0]], [[4kx1\|4kx1]]</td></tr>~~	+
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kx2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kx2 RCSB], [http://www.ebi.ac.uk/pdbsum/4kx2 PDBsum]</span></td></tr>	+
-	~~<table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~Maintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNASufA6~~ and N1-methylguanosine at position 37 (m1G37) modification-deficient anticodon stem loopPro, both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNASufA6 to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m1G37 modification of tRNAPro also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by ~~the ribosome in maintaining the correct translational reading frame.~~	+
-		+
-	Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.,Maehigashi T, Dunkle JA, Miles SJ, Dunham CM Proc Natl Acad Sci U S A. 2014 Aug 15. pii: 201409436. PMID:25128388<ref>PMID:25128388</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Thermus thermophilus]]~~	+
-	~~[[Category: Dunham, C M.]]~~	+
-	~~[[Category: Dunkle, J A.]]~~	+
-	~~[[Category: Maehigashi, T.]]~~	+
-	~~[[Category: 16]]~~	+
-	~~[[Category: 23]]~~	+
-	~~[[Category: 30]]~~	+
-	~~[[Category: 70]]~~	+
-	~~[[Category: Protein biosynthesis]]~~	+
-	~~[[Category: Ribosomal subunit]]~~	+
-	~~[[Category: Ribosome]]~~	+
-	~~[[Category: Ribosome]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Transfer rna]]~~	+
-	~~[[Category: Trna]]~~	+

OCA at 03:10, 4 September 2014

2014-09-04T03:10:00Z

←Older revision		Revision as of 03:10, 4 September 2014
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	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kx2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kx2 RCSB], [http://www.ebi.ac.uk/pdbsum/4kx2 PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kx2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kx2 RCSB], [http://www.ebi.ac.uk/pdbsum/4kx2 PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Maintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNASufA6 and N1-methylguanosine at position 37 (m1G37) modification-deficient anticodon stem loopPro, both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNASufA6 to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m1G37 modification of tRNAPro also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by the ribosome in maintaining the correct translational reading frame.
		+
		+	Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.,Maehigashi T, Dunkle JA, Miles SJ, Dunham CM Proc Natl Acad Sci U S A. 2014 Aug 15. pii: 201409436. PMID:25128388<ref>PMID:25128388</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 02:40, 7 August 2014

2014-08-07T02:40:36Z

←Older revision		Revision as of 02:40, 7 August 2014
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-	'''~~Unreleased structure~~'''	+	==Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome==
-		+	<StructureSection load='4kx2' size='340' side='right' caption='[[4kx2]], [[Resolution\|resolution]] 3.44Å' scene=''>
-	~~The entry~~ 4kx2 is ~~ON HOLD until Aug 14 2015~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4kx2]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KX2 FirstGlance]. <br>
-	~~Authors~~: ~~Maehigashi~~, T., ~~Dunkle~~, J.A., Dunham, C.M.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
-		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPU:PUROMYCIN-5-MONOPHOSPHATE'>PPU</scene></td></tr>
-	~~Description~~: ~~Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the~~ Ribosome	+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4kwz\|4kwz]], [[4kx0\|4kx0]], [[4kx1\|4kx1]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kx2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kx2 RCSB], [http://www.ebi.ac.uk/pdbsum/4kx2 PDBsum]</span></td></tr>
		+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Thermus thermophilus]]
		+	[[Category: Dunham, C M.]]
		+	[[Category: Dunkle, J A.]]
		+	[[Category: Maehigashi, T.]]
		+	[[Category: 16]]
		+	[[Category: 23]]
		+	[[Category: 30]]
		+	[[Category: 70]]
		+	[[Category: Protein biosynthesis]]
		+	[[Category: Ribosomal subunit]]
		+	[[Category: Ribosome]]
		+	[[Category: Ribosome]]
		+	[[Category: Rna]]
		+	[[Category: Transfer rna]]
		+	[[Category: Trna]]

OCA at 06:55, 23 July 2014

2014-07-23T06:55:32Z

←Older revision		Revision as of 06:55, 23 July 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4kx2 is ON HOLD until ~~Jul 21~~ 2015	+	The entry 4kx2 is ON HOLD until Aug 14 2015

	Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.		Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.

	Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome		Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome

OCA at 09:33, 16 July 2014

2014-07-16T09:33:14Z

←Older revision		Revision as of 09:33, 16 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4kx2 is ON HOLD until Jul 15 2015	+	The entry 4kx2 is ON HOLD until Jul 21 2015

	Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.		Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.

	Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome		Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome

OCA at 07:39, 28 May 2014

2014-05-28T07:39:44Z

←Older revision		Revision as of 07:39, 28 May 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4kx2 is ON HOLD until ~~Paper Publication~~	+	The entry 4kx2 is ON HOLD until Jul 15 2015

	Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.		Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.

	Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome		Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome

OCA at 04:12, 1 August 2013

2013-08-01T04:12:48Z

←Older revision		Revision as of 04:12, 1 August 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4kx2 is ON HOLD	+	The entry 4kx2 is ON HOLD until Paper Publication

	Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.		Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.

	Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome		Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome

OCA: Protected "4kx2" [edit=sysop:move=sysop]

2013-06-19T12:10:09Z

Protected "4kx2" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:10, 19 June 2013

OCA: New page: '''Unreleased structure''' The entry 4kx2 is ON HOLD Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M. Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Cod...

2013-06-19T12:10:08Z

New page: '''Unreleased structure''' The entry 4kx2 is ON HOLD Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M. Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Cod...

New page

'''Unreleased structure'''

The entry 4kx2 is ON HOLD

Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.

Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 bound to Codon CCC-G on the Ribosome