4lli - Revision history

OCA at 12:20, 1 March 2024

2024-03-01T12:20:21Z

←Older revision		Revision as of 12:20, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4lli]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LLI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4lli]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LLI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lli OCA], [https://pdbe.org/4lli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lli RCSB], [https://www.ebi.ac.uk/pdbsum/4lli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lli ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lli OCA], [https://pdbe.org/4lli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lli RCSB], [https://www.ebi.ac.uk/pdbsum/4lli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lli ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 10:		Line 11:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.<ref>PMID:10448864</ref>		[https://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.<ref>PMID:10448864</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Vertebrate type V myosins (MyoV) Myo5a, Myo5b, and Myo5c mediate transport of several different cargoes. All MyoV paralogs bind to cargo complexes mainly by their C-terminal globular domains. In absence of cargo, the globular domain of Myo5a inhibits its motor domain. Here, we report low-resolution SAXS models for the globular domains from human Myo5a, Myo5b, and Myo5c, which suggest very similar overall shapes of all three paralogs. We determined the crystal structures of globular domains from Myo5a and Myo5b, and provide a homology model for human Myo5c. When we docked the Myo5a crystal structure into a previously published electron microscopy density of the autoinhibited full-length Myo5a, only one domain orientation resulted in a good fit. This structural arrangement suggests the participation of additional region of the globular domain in autoinhibition. Quantification of the interaction of the Myo5a globular domain with its motor complex revealed a tight binding with dissociation half-life in the order of minutes, suggesting a rather slow transition between the active and inactive states.
-
-	Structural insights into the globular tails of the human type v myosins myo5a, myo5b, and myo5c.,Velvarska H, Niessing D PLoS One. 2013 Dec 10;8(12):e82065. doi: 10.1371/journal.pone.0082065. PMID:24339992<ref>PMID:24339992</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4lli" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:08, 14 December 2022

2022-12-14T11:08:52Z

←Older revision		Revision as of 11:08, 14 December 2022
Line 1:		Line 1:

	==Crystal Structure of human Myosin 5a globular domain==		==Crystal Structure of human Myosin 5a globular domain==
-	<StructureSection load='4lli' size='340' side='right' caption='[[4lli]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='4lli' size='340' side='right'caption='[[4lli]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4lli]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LLI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4LLI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4lli]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LLI FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lnz\|4lnz]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lli OCA], [https://pdbe.org/4lli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lli RCSB], [https://www.ebi.ac.uk/pdbsum/4lli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lli ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MYH12, MYO5A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4lli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lli OCA], [~~http~~://pdbe.org/4lli PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4lli RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4lli PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4lli ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN]] Griscelli disease type 3;Neuroectodermal melanolysosomal disease;Griscelli disease type 1. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN] Griscelli disease type 3;Neuroectodermal melanolysosomal disease;Griscelli disease type 1. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN]] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.<ref>PMID:10448864</ref>	+	[https://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.<ref>PMID:10448864</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4lli" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4lli" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Myosin\|Myosin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Niessing, D~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Velvarska, H~~]]	+	[[Category: Niessing D]]
-	[[Category: ~~Dilute domain]]~~	+	[[Category: Velvarska H]]
-	~~[[Category: Globular tail]]~~	+
-	~~[[Category: Motor protein]]~~	+
-	~~[[Category: Type v myosin~~]]	+

OCA at 03:05, 5 August 2016

2016-08-05T03:05:25Z

←Older revision		Revision as of 03:05, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of human Myosin 5a globular domain==		==Crystal Structure of human Myosin 5a globular domain==
	<StructureSection load='4lli' size='340' side='right' caption='[[4lli]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4lli' size='340' side='right' caption='[[4lli]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lnz\|4lnz]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lnz\|4lnz]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MYH12, MYO5A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MYH12, MYO5A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lli RCSB], [http://www.ebi.ac.uk/pdbsum/4lli PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lli OCA], [http://pdbe.org/4lli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lli RCSB], [http://www.ebi.ac.uk/pdbsum/4lli PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lli ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4lli" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 16:53, 21 December 2014

2014-12-21T16:53:52Z

←Older revision		Revision as of 16:53, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4lli\| PDB=4lli \| SCENE= }}~~	+	==Crystal Structure of human Myosin 5a globular domain==
-	===Crystal Structure of human Myosin 5a globular domain===	+	<StructureSection load='4lli' size='340' side='right' caption='[[4lli]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24339992}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4lli]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LLI FirstGlance]. <br>
-	==Disease==	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4lnz\|4lnz]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MYH12, MYO5A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lli OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lli RCSB], [http://www.ebi.ac.uk/pdbsum/4lli PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
	[[http://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN]] Griscelli disease type 3;Neuroectodermal melanolysosomal disease;Griscelli disease type 1. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.		[[http://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN]] Griscelli disease type 3;Neuroectodermal melanolysosomal disease;Griscelli disease type 1. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN]] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.<ref>PMID:10448864</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Vertebrate type V myosins (MyoV) Myo5a, Myo5b, and Myo5c mediate transport of several different cargoes. All MyoV paralogs bind to cargo complexes mainly by their C-terminal globular domains. In absence of cargo, the globular domain of Myo5a inhibits its motor domain. Here, we report low-resolution SAXS models for the globular domains from human Myo5a, Myo5b, and Myo5c, which suggest very similar overall shapes of all three paralogs. We determined the crystal structures of globular domains from Myo5a and Myo5b, and provide a homology model for human Myo5c. When we docked the Myo5a crystal structure into a previously published electron microscopy density of the autoinhibited full-length Myo5a, only one domain orientation resulted in a good fit. This structural arrangement suggests the participation of additional region of the globular domain in autoinhibition. Quantification of the interaction of the Myo5a globular domain with its motor complex revealed a tight binding with dissociation half-life in the order of minutes, suggesting a rather slow transition between the active and inactive states.

-	~~==Function==~~	+	Structural insights into the globular tails of the human type v myosins myo5a, myo5b, and myo5c.,Velvarska H, Niessing D PLoS One. 2013 Dec 10;8(12):e82065. doi: 10.1371/journal.pone.0082065. PMID:24339992<ref>PMID:24339992</ref>
-	~~[[http~~:~~//www~~.~~uniprot~~.~~org~~/~~uniprot/MYO5A_HUMAN MYO5A_HUMAN]] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament~~. ~~Involved in melanosome transport~~. ~~Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation~~.<ref>PMID:~~10448864~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4lli]] is~~ a ~~2 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4LLI OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:024339992</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Myosin\|Myosin]]
-	[[Category: Niessing, D.]]	+	== References ==
-	[[Category: Velvarska, H.]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Niessing, D]]
		+	[[Category: Velvarska, H]]
	[[Category: Dilute domain]]		[[Category: Dilute domain]]
	[[Category: Globular tail]]		[[Category: Globular tail]]
	[[Category: Motor protein]]		[[Category: Motor protein]]
	[[Category: Type v myosin]]		[[Category: Type v myosin]]

OCA at 07:13, 15 January 2014

2014-01-15T07:13:56Z

←Older revision		Revision as of 07:13, 15 January 2014
Line 1:		Line 1:
	{{STRUCTURE_4lli\| PDB=4lli \| SCENE= }}		{{STRUCTURE_4lli\| PDB=4lli \| SCENE= }}
	===Crystal Structure of human Myosin 5a globular domain===		===Crystal Structure of human Myosin 5a globular domain===
		+	{{ABSTRACT_PUBMED_24339992}}

	==Disease==		==Disease==
Line 12:		Line 13:

	==Reference==		==Reference==
-	<references group="xtra"/><references/>	+	<ref group="xtra">PMID:024339992</ref><references group="xtra"/><references/>
	[[Category: Niessing, D.]]		[[Category: Niessing, D.]]
	[[Category: Velvarska, H.]]		[[Category: Velvarska, H.]]

OCA at 10:27, 25 December 2013

2013-12-25T10:27:35Z

←Older revision		Revision as of 10:27, 25 December 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4lli\| PDB=4lli \| SCENE= }}
		+	===Crystal Structure of human Myosin 5a globular domain===

-	The entry ~~4lli~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Disease==
		+	[[http://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN]] Griscelli disease type 3;Neuroectodermal melanolysosomal disease;Griscelli disease type 1. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.

-	~~Authors~~: ~~Velvarska, H~~.~~, Niessing, D~~.	+	==Function==
		+	[[http://www.uniprot.org/uniprot/MYO5A_HUMAN MYO5A_HUMAN]] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.<ref>PMID:10448864</ref>

-	~~Description: Crystal~~ Structure ~~of human Myosin 5a globular~~ domain	+	==About this Structure==
		+	[[4lli]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LLI OCA].
		+
		+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Niessing, D.]]
		+	[[Category: Velvarska, H.]]
		+	[[Category: Dilute domain]]
		+	[[Category: Globular tail]]
		+	[[Category: Motor protein]]
		+	[[Category: Type v myosin]]

OCA at 05:55, 23 October 2013

2013-10-23T05:55:27Z

←Older revision		Revision as of 05:55, 23 October 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4lli is ON HOLD	+	The entry 4lli is ON HOLD until Paper Publication

	Authors: Velvarska, H., Niessing, D.		Authors: Velvarska, H., Niessing, D.

-	Description: ~~Human myosin V cargo binding~~ domain	+	Description: Crystal Structure of human Myosin 5a globular domain

OCA: Protected "4lli" [edit=sysop:move=sysop]

2013-07-18T04:47:50Z

Protected "4lli" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:47, 18 July 2013

OCA: New page: '''Unreleased structure''' The entry 4lli is ON HOLD Authors: Velvarska, H., Niessing, D. Description: Human myosin V cargo binding domain

2013-07-18T04:47:49Z

New page: '''Unreleased structure''' The entry 4lli is ON HOLD Authors: Velvarska, H., Niessing, D. Description: Human myosin V cargo binding domain

New page

'''Unreleased structure'''

The entry 4lli is ON HOLD

Authors: Velvarska, H., Niessing, D.

Description: Human myosin V cargo binding domain