4lx3 - Revision history

OCA at 10:47, 31 August 2022

2022-08-31T10:47:27Z

←Older revision		Revision as of 10:47, 31 August 2022
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	==Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein==		==Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein==
-	<StructureSection load='4lx3' size='340' side='right'caption='[[4lx3]]~~, [[Resolution\|resolution]] 1.50Å~~' scene=''>	+	<StructureSection load='4lx3' size='340' side='right'caption='[[4lx3]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[4lx3]] is a 2 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LX3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LX3 FirstGlance]. <br>
-	</td></tr>~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [https://pdbe.org/4lx3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [https://www.ebi.ac.uk/pdbsum/4lx3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lx3 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [https://pdbe.org/4lx3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [https://www.ebi.ac.uk/pdbsum/4lx3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lx3 ProSAT]</span></td></tr>	+
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The first crystal trans-structure of a naturally occurring split intein has been determined for the Npu DnaE split intein. Guided by this structure, the residues NArg50 and CSer35, well conserved in DnaE split inteins, are identified to be critical in the trans-splicing of Npu DnaE split intein. An in vitro splicing assay demonstrates that NArg50 and CSer35 play synergistic roles in modulating its intein activity. The C-terminal asparagine CAsn36 exhibits two orientations of its side-chain and interacts with both NArg50 and CSer35 through H-bonding. These interactions likely facilitate cyclization of Asn in the course of protein splicing. The mutation of either residue reduces intein activity, and correlates with the low activity of the Ssp DnaE split intein. On the other hand, NArg50 also forms a H-bond with the highly conserved F-block aspartate CAsp17, thus influencing the N-S acyl shift during N-terminal cleavage. Sequence alignments show that residues NArg50 and CSer35 are rather conserved in those split inteins that lack a penultimate histidine residue. The conserved non-catalytic residues of split inteins modulate the efficiency of protein trans-splicing by H-bond interactions with the catalytic residues at the splice junction.
-
-	Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein.,Wu Q, Gao ZQ, Wei Y, Ma G, Zheng Y, Dong YH, Liu Y Biochem J. 2014 Apr 23. PMID:24758175<ref>PMID:24758175</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4lx3" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: DNA-directed DNA polymerase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dong, Y]]	+	[[Category: Dong Y]]
-	[[Category: Gao, Z]]	+	[[Category: Gao Z]]
-	[[Category: Liu, Y]]	+	[[Category: Liu Y]]
-	[[Category: Ma, G]]	+	[[Category: Ma G]]
-	[[Category: Wei, Y]]	+	[[Category: Wei Y]]
-	[[Category: Wu, Q]]	+	[[Category: Wu Q]]
-	[[Category: Zheng, Y]]	+	[[Category: Zheng Y]]
-	~~[[Category: Naturally occurring]]~~	+
-	~~[[Category: Trans-structure]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 05:03, 25 August 2022

2022-08-25T05:03:50Z

←Older revision		Revision as of 05:03, 25 August 2022
Line 3:		Line 3:
	<StructureSection load='4lx3' size='340' side='right'caption='[[4lx3]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='4lx3' size='340' side='right'caption='[[4lx3]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4lx3]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Nosp7 Nosp7]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LX3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4LX3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4lx3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LX3 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Npun_F4872 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63737 NOSP7]), Npun_F5684 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63737 NOSP7])</td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [https://pdbe.org/4lx3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [https://www.ebi.ac.uk/pdbsum/4lx3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lx3 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [~~http~~://pdbe.org/4lx3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4lx3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4lx3 ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 4lx3" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4lx3" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[DNA polymerase\|DNA polymerase]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 23:
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Nosp7]]
	[[Category: Dong, Y]]		[[Category: Dong, Y]]
	[[Category: Gao, Z]]		[[Category: Gao, Z]]

OCA at 09:04, 3 April 2019

2019-04-03T09:04:34Z

←Older revision		Revision as of 09:04, 3 April 2019
Line 1:		Line 1:

	==Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein==		==Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein==
-	<StructureSection load='4lx3' size='340' side='right' caption='[[4lx3]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='4lx3' size='340' side='right'caption='[[4lx3]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4lx3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LX3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4lx3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nosp7 Nosp7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LX3 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Npun_F4872 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63737 NOSP7]), Npun_F5684 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63737 NOSP7])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [http://pdbe.org/4lx3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [http://www.ebi.ac.uk/pdbsum/4lx3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lx3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [http://pdbe.org/4lx3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [http://www.ebi.ac.uk/pdbsum/4lx3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lx3 ProSAT]</span></td></tr>
Line 25:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
		+	[[Category: Large Structures]]
		+	[[Category: Nosp7]]
	[[Category: Dong, Y]]		[[Category: Dong, Y]]
	[[Category: Gao, Z]]		[[Category: Gao, Z]]

OCA at 18:01, 11 August 2016

2016-08-11T18:01:35Z

←Older revision		Revision as of 18:01, 11 August 2016
Line 1:		Line 1:
		+
	==Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein==		==Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein==
	<StructureSection load='4lx3' size='340' side='right' caption='[[4lx3]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='4lx3' size='340' side='right' caption='[[4lx3]], [[Resolution\|resolution]] 1.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [http://www.ebi.ac.uk/pdbsum/4lx3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [http://pdbe.org/4lx3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [http://www.ebi.ac.uk/pdbsum/4lx3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lx3 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4lx3" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA polymerase\|DNA polymerase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:46, 5 January 2015

2015-01-05T08:46:49Z

←Older revision		Revision as of 08:46, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4lx3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LX3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4lx3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LX3 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [http://www.ebi.ac.uk/pdbsum/4lx3 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [http://www.ebi.ac.uk/pdbsum/4lx3 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
-	[[Category: Dong, Y.]]	+	[[Category: Dong, Y]]
-	[[Category: Gao, Z.]]	+	[[Category: Gao, Z]]
-	[[Category: Liu, Y.]]	+	[[Category: Liu, Y]]
-	[[Category: Ma, G.]]	+	[[Category: Ma, G]]
-	[[Category: Wei, Y.]]	+	[[Category: Wei, Y]]
-	[[Category: Wu, Q.]]	+	[[Category: Wu, Q]]
-	[[Category: Zheng, Y.]]	+	[[Category: Zheng, Y]]
	[[Category: Naturally occurring]]		[[Category: Naturally occurring]]
	[[Category: Trans-structure]]		[[Category: Trans-structure]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 06:26, 25 June 2014

2014-06-25T06:26:59Z

←Older revision		Revision as of 06:26, 25 June 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein==
		+	<StructureSection load='4lx3' size='340' side='right' caption='[[4lx3]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4lx3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LX3 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lx3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lx3 RCSB], [http://www.ebi.ac.uk/pdbsum/4lx3 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The first crystal trans-structure of a naturally occurring split intein has been determined for the Npu DnaE split intein. Guided by this structure, the residues NArg50 and CSer35, well conserved in DnaE split inteins, are identified to be critical in the trans-splicing of Npu DnaE split intein. An in vitro splicing assay demonstrates that NArg50 and CSer35 play synergistic roles in modulating its intein activity. The C-terminal asparagine CAsn36 exhibits two orientations of its side-chain and interacts with both NArg50 and CSer35 through H-bonding. These interactions likely facilitate cyclization of Asn in the course of protein splicing. The mutation of either residue reduces intein activity, and correlates with the low activity of the Ssp DnaE split intein. On the other hand, NArg50 also forms a H-bond with the highly conserved F-block aspartate CAsp17, thus influencing the N-S acyl shift during N-terminal cleavage. Sequence alignments show that residues NArg50 and CSer35 are rather conserved in those split inteins that lack a penultimate histidine residue. The conserved non-catalytic residues of split inteins modulate the efficiency of protein trans-splicing by H-bond interactions with the catalytic residues at the splice junction.

-	~~The entry 4lx3 is ON HOLD until Paper Publication~~	+	Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein.,Wu Q, Gao ZQ, Wei Y, Ma G, Zheng Y, Dong YH, Liu Y Biochem J. 2014 Apr 23. PMID:24758175<ref>PMID:24758175</ref>

-	~~Authors: Wu~~, Q., Gao, Z., ~~Wei, y~~., Ma, G.~~, Zheng~~, Y., ~~Dong, Y~~.~~, Liu~~, Y.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Conserved Residues that Modulate Protein trans~~-~~Splicing of Npu DnaE Split Intein~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: DNA-directed DNA polymerase]]
		+	[[Category: Dong, Y.]]
		+	[[Category: Gao, Z.]]
		+	[[Category: Liu, Y.]]
		+	[[Category: Ma, G.]]
		+	[[Category: Wei, Y.]]
		+	[[Category: Wu, Q.]]
		+	[[Category: Zheng, Y.]]
		+	[[Category: Naturally occurring]]
		+	[[Category: Trans-structure]]
		+	[[Category: Transferase]]

OCA at 06:05, 18 June 2014

2014-06-18T06:05:53Z

←Older revision		Revision as of 06:05, 18 June 2014
Line 3:		Line 3:
	The entry 4lx3 is ON HOLD until Paper Publication		The entry 4lx3 is ON HOLD until Paper Publication

-	Authors: Wu, Q., Gao, Z., Wei, y., Dong, Y., Liu, Y.	+	Authors: Wu, Q., Gao, Z., Wei, y., Ma, G., Zheng, Y., Dong, Y., Liu, Y.

-	Description: ~~Crystal structure~~ of DnaE ~~intein from Nostoc punctiforme in split forms~~	+	Description: Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein

OCA at 04:45, 12 September 2013

2013-09-12T04:45:24Z

←Older revision		Revision as of 04:45, 12 September 2013
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4lx3 is ON HOLD	+	The entry 4lx3 is ON HOLD until Paper Publication

	Authors: Wu, Q., Gao, Z., Wei, y., Dong, Y., Liu, Y.		Authors: Wu, Q., Gao, Z., Wei, y., Dong, Y., Liu, Y.

	Description: Crystal structure of DnaE intein from Nostoc punctiforme in split forms		Description: Crystal structure of DnaE intein from Nostoc punctiforme in split forms

OCA at 07:53, 21 August 2013

2013-08-21T07:53:16Z

←Older revision		Revision as of 07:53, 21 August 2013
Line 3:		Line 3:
	The entry 4lx3 is ON HOLD		The entry 4lx3 is ON HOLD

-	Authors: wu, ~~qin~~, ~~gao~~, ~~zengqiang~~, ~~wei~~, ~~yong~~, ~~dong~~, ~~yuhui~~ , ~~liu~~, ~~yangzhong~~	+	Authors: Wu, Q., Gao, Z., Wei, y., Dong, Y., Liu, Y.

	Description: Crystal structure of DnaE intein from Nostoc punctiforme in split forms		Description: Crystal structure of DnaE intein from Nostoc punctiforme in split forms

OCA: Protected "4lx3" [edit=sysop:move=sysop]

2013-08-07T20:19:10Z

Protected "4lx3" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:19, 7 August 2013