4m7g - Revision history

OCA at 16:33, 20 September 2023

2023-09-20T16:33:34Z

←Older revision		Revision as of 16:33, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4m7g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M7G FirstGlance]. <br>		<table><tr><td colspan='2'>[[4m7g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M7G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m7g OCA], [https://pdbe.org/4m7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m7g RCSB], [https://www.ebi.ac.uk/pdbsum/4m7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m7g ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 0.81Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m7g OCA], [https://pdbe.org/4m7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m7g RCSB], [https://www.ebi.ac.uk/pdbsum/4m7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m7g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 11:01, 21 December 2022

2022-12-21T11:01:20Z

←Older revision		Revision as of 11:01, 21 December 2022
Line 3:		Line 3:
	<StructureSection load='4m7g' size='340' side='right'caption='[[4m7g]], [[Resolution\|resolution]] 0.81Å' scene=''>		<StructureSection load='4m7g' size='340' side='right'caption='[[4m7g]], [[Resolution\|resolution]] 0.81Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4m7g]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4M7G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4m7g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M7G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4m7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m7g OCA], [~~http~~://pdbe.org/4m7g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4m7g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4m7g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4m7g ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m7g OCA], [https://pdbe.org/4m7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m7g RCSB], [https://www.ebi.ac.uk/pdbsum/4m7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m7g ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TRYP_SACER TRYP_SACER]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Blankenship, E]]	+	[[Category: Saccharopolyspora erythraea]]
-	[[Category: Lodowski~~, D T~~]]	+	[[Category: Blankenship E]]
-	[[Category: Miyagi, M]]	+	[[Category: Lodowski DT]]
-	[[Category: Vukoti, K]]	+	[[Category: Miyagi M]]
-	~~[[Category: Hydrolase]]~~	+	[[Category: Vukoti K]]
-	~~[[Category: Serine protease~~]]	+

OCA at 05:07, 13 February 2020

2020-02-13T05:07:48Z

←Older revision		Revision as of 05:07, 13 February 2020
Line 1:		Line 1:

	==Streptomyces Erythraeus Trypsin==		==Streptomyces Erythraeus Trypsin==
-	<StructureSection load='4m7g' size='340' side='right' caption='[[4m7g]], [[Resolution\|resolution]] 0.81Å' scene=''>	+	<StructureSection load='4m7g' size='340' side='right'caption='[[4m7g]], [[Resolution\|resolution]] 0.81Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4m7g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M7G FirstGlance]. <br>		<table><tr><td colspan='2'>[[4m7g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M7G FirstGlance]. <br>
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Blankenship, E]]		[[Category: Blankenship, E]]
	[[Category: Lodowski, D T]]		[[Category: Lodowski, D T]]

OCA at 19:49, 11 August 2016

2016-08-11T19:49:18Z

←Older revision		Revision as of 19:49, 11 August 2016
Line 1:		Line 1:
		+
	==Streptomyces Erythraeus Trypsin==		==Streptomyces Erythraeus Trypsin==
	<StructureSection load='4m7g' size='340' side='right' caption='[[4m7g]], [[Resolution\|resolution]] 0.81Å' scene=''>		<StructureSection load='4m7g' size='340' side='right' caption='[[4m7g]], [[Resolution\|resolution]] 0.81Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4m7g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M7G FirstGlance]. <br>		<table><tr><td colspan='2'>[[4m7g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M7G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m7g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m7g RCSB], [http://www.ebi.ac.uk/pdbsum/4m7g PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m7g OCA], [http://pdbe.org/4m7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m7g RCSB], [http://www.ebi.ac.uk/pdbsum/4m7g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m7g ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 13:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4m7g" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:15, 20 January 2015

2015-01-20T10:15:28Z

←Older revision		Revision as of 10:15, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4m7g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M7G FirstGlance]. <br>		<table><tr><td colspan='2'>[[4m7g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M7G FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m7g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m7g RCSB], [http://www.ebi.ac.uk/pdbsum/4m7g PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m7g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m7g RCSB], [http://www.ebi.ac.uk/pdbsum/4m7g PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Blankenship, E.]]	+	[[Category: Blankenship, E]]
-	[[Category: Lodowski, D T.]]	+	[[Category: Lodowski, D T]]
-	[[Category: Miyagi, M.]]	+	[[Category: Miyagi, M]]
-	[[Category: Vukoti, K.]]	+	[[Category: Vukoti, K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

OCA at 22:20, 1 October 2014

2014-10-01T22:20:52Z

←Older revision		Revision as of 22:20, 1 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4m7g\| PDB~~=4m7g \| ~~SCENE~~= }}	+	==Streptomyces Erythraeus Trypsin==
-	===~~Streptomyces Erythraeus Trypsin~~===	+	<StructureSection load='4m7g' size='340' side='right' caption='[[4m7g]], [[Resolution\|resolution]] 0.81Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4m7g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M7G FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m7g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m7g RCSB], [http://www.ebi.ac.uk/pdbsum/4m7g PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	With more than 500 crystal structures determined, serine proteases make up greater than one-third of all proteases structurally examined to date, making them among the best biochemically and structurally characterized enzymes. Despite the numerous crystallographic and biochemical studies of trypsin and related serine proteases, there are still considerable shortcomings in the understanding of their catalytic mechanism. Streptomyces erythraeus trypsin (SET) does not exhibit autolysis and crystallizes readily at physiological pH; hence, it is well suited for structural studies aimed at extending the understanding of the catalytic mechanism of serine proteases. While X-ray crystallographic structures of this enzyme have been reported, no coordinates have ever been made available in the Protein Data Bank. Based on this, and observations on the extreme stability and unique properties of this particular trypsin, it was decided to crystallize it and determine its structure. Here, the first sub-angstrom resolution structure of an unmodified, unliganded trypsin crystallized at physiological pH is reported. Detailed structural analysis reveals the geometry and structural rigidity of the catalytic triad in the unoccupied active site and comparison to related serine proteases provides a context for interpretation of biochemical studies of catalytic mechanism and activity.

-	~~==About this Structure==~~	+	Conformational flexibility in the catalytic triad revealed by the high-resolution crystal structure of Streptomyces erythraeus trypsin in an unliganded state.,Blankenship E, Vukoti K, Miyagi M, Lodowski DT Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):833-40. doi:, 10.1107/S1399004713033658. Epub 2014 Feb 22. PMID:24598752<ref>PMID:24598752</ref>
-	~~[[4m7g]] is a 1 chain~~ structure. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id~~=~~4M7G OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Blankenship, E.]]		[[Category: Blankenship, E.]]
	[[Category: Lodowski, D T.]]		[[Category: Lodowski, D T.]]

OCA at 13:59, 12 March 2014

2014-03-12T13:59:59Z

←Older revision		Revision as of 13:59, 12 March 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4m7g\| PDB=4m7g \| SCENE= }}
		+	===Streptomyces Erythraeus Trypsin===

-	~~The entry~~ 4m7g is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4m7g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M7G OCA].
-	~~Authors~~: Blankenship, E., ~~Vukoti, K~~., Miyagi, M., ~~Lodowski, D.T~~.	+	[[Category: Blankenship, E.]]
-		+	[[Category: Lodowski, D T.]]
-	~~Description~~: ~~Streptomyces Erythraeus Trypsin~~	+	[[Category: Miyagi, M.]]
		+	[[Category: Vukoti, K.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Serine protease]]

OCA at 12:49, 20 November 2013

2013-11-20T12:49:36Z

←Older revision		Revision as of 12:49, 20 November 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4m7g is ON HOLD ~~until Paper Publication~~	+	The entry 4m7g is ON HOLD

	Authors: Blankenship, E., Vukoti, K., Miyagi, M., Lodowski, D.T.		Authors: Blankenship, E., Vukoti, K., Miyagi, M., Lodowski, D.T.

	Description: Streptomyces Erythraeus Trypsin		Description: Streptomyces Erythraeus Trypsin

OCA at 07:34, 29 September 2013

2013-09-29T07:34:04Z

←Older revision		Revision as of 07:34, 29 September 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4m7g is ON HOLD	+	The entry 4m7g is ON HOLD until Paper Publication

	Authors: Blankenship, E., Vukoti, K., Miyagi, M., Lodowski, D.T.		Authors: Blankenship, E., Vukoti, K., Miyagi, M., Lodowski, D.T.

-	Description: Trypsin	+	Description: Streptomyces Erythraeus Trypsin

OCA: Protected "4m7g" [edit=sysop:move=sysop]

2013-08-21T07:57:39Z

Protected "4m7g" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:57, 21 August 2013