4m9q - Revision history

OCA at 16:34, 20 September 2023

2023-09-20T16:34:42Z

←Older revision		Revision as of 16:34, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4m9q]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M9Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M9Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[4m9q]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M9Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M9Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m9q OCA], [https://pdbe.org/4m9q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m9q RCSB], [https://www.ebi.ac.uk/pdbsum/4m9q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m9q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m9q OCA], [https://pdbe.org/4m9q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m9q RCSB], [https://www.ebi.ac.uk/pdbsum/4m9q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m9q ProSAT]</span></td></tr>
	</table>		</table>

OCA at 11:04, 21 December 2022

2022-12-21T11:04:40Z

←Older revision		Revision as of 11:04, 21 December 2022
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	==Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp==		==Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp==
-	<StructureSection load='4m9q' size='340' side='right' caption='[[4m9q]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4m9q' size='340' side='right'caption='[[4m9q]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4m9q]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Chlre Chlre~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M9Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4M9Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4m9q]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M9Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M9Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARL13, CHLREDRAFT_195529 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m9q OCA], [https://pdbe.org/4m9q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m9q RCSB], [https://www.ebi.ac.uk/pdbsum/4m9q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m9q ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4m9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m9q OCA], [~~http~~://pdbe.org/4m9q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4m9q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4m9q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4m9q ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ARL13_CHLRE ARL13_CHLRE] Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chlre~~]]	+	[[Category: Chlamydomonas reinhardtii]]
-	[[Category: ~~Small monomeric GTPase~~]]	+	[[Category: Large Structures]]
-	[[Category: Koerner, C]]	+	[[Category: Koerner C]]
-	[[Category: Miertzschke, M]]	+	[[Category: Miertzschke M]]
-	[[Category: Spoerner, M]]	+	[[Category: Spoerner M]]
-	[[Category: Wittinghofer, A]]	+	[[Category: Wittinghofer A]]
-	~~[[Category: Cilia]]~~	+
-	~~[[Category: G domain]]~~	+
-	~~[[Category: Gtpase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Joubert syndrome~~]]	+

OCA at 08:29, 11 August 2016

2016-08-11T08:29:33Z

←Older revision		Revision as of 08:29, 11 August 2016
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		+
	==Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp==		==Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp==
	<StructureSection load='4m9q' size='340' side='right' caption='[[4m9q]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4m9q' size='340' side='right' caption='[[4m9q]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARL13, CHLREDRAFT_195529 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARL13, CHLREDRAFT_195529 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m9q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m9q RCSB], [http://www.ebi.ac.uk/pdbsum/4m9q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m9q OCA], [http://pdbe.org/4m9q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m9q RCSB], [http://www.ebi.ac.uk/pdbsum/4m9q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m9q ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4m9q" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:30, 5 January 2015

2015-01-05T09:30:04Z

←Older revision		Revision as of 09:30, 5 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4m9q\| PDB=4m9q \| SCENE= }}~~	+	==Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp==
-	===Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp===	+	<StructureSection load='4m9q' size='340' side='right' caption='[[4m9q]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24168557}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4m9q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M9Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M9Q FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARL13, CHLREDRAFT_195529 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m9q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m9q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m9q RCSB], [http://www.ebi.ac.uk/pdbsum/4m9q PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ciliopathies are human diseases arising from defects in primary or motile cilia. The small G protein Arl13B localizes to microtubule doublets of the ciliary axoneme and is mutated in Joubert Syndrome. Its GDP-GTP mechanistic cycle and the impact of patient mutations remain elusive. Here, we applied high resolution structural and biochemical approaches to study Arl13B. The crystal structure of Chlamydomonas rheinhardtii Arl13B comprising Gdomain and part of its unique C-terminus and biochemical experiments reveal the lack of intrinsic hydrolysis and the absence of a catalytic residue in the active site. The structure shows residues representing patient mutations R79Q and R200C to be involved in stabilizing important intramolecular interactions. Our studies suggest that Arg79 is crucial for the GDPGTP conformational change by stabilizing the large two residue register shift typical for Arf and Arl subfamily proteins. A corresponding mutation in Arl3 induces considerable defects in effector and GAP binding suggesting a loss of Arl13B function in Joubert Syndrome patients.

-	~~==About this Structure==~~	+	Structural insights into the small G protein Arl13B and implications for Joubert syndrome.,Miertzschke M, Koerner C, Spoerner M, Wittinghofer A Biochem J. 2013 Oct 30. PMID:24168557<ref>PMID:24168557</ref>
-	~~[[4m9q]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Chlre Chlre]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4M9Q OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024168557</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Chlre]]		[[Category: Chlre]]
	[[Category: Small monomeric GTPase]]		[[Category: Small monomeric GTPase]]
-	[[Category: Koerner, C.]]	+	[[Category: Koerner, C]]
-	[[Category: Miertzschke, M.]]	+	[[Category: Miertzschke, M]]
-	[[Category: Spoerner, M.]]	+	[[Category: Spoerner, M]]
-	[[Category: Wittinghofer, A.]]	+	[[Category: Wittinghofer, A]]
	[[Category: Cilia]]		[[Category: Cilia]]
	[[Category: G domain]]		[[Category: G domain]]

OCA at 06:49, 13 November 2013

2013-11-13T06:49:09Z

←Older revision		Revision as of 06:49, 13 November 2013
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	{{STRUCTURE_4m9q\| PDB=4m9q \| SCENE= }}		{{STRUCTURE_4m9q\| PDB=4m9q \| SCENE= }}
	===Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp===		===Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp===
		+	{{ABSTRACT_PUBMED_24168557}}

	==About this Structure==		==About this Structure==
-	[[4m9q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chlamydomonas_reinhardtii Chlamydomonas reinhardtii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M9Q OCA].	+	[[4m9q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M9Q OCA].
-	[[Category: ~~Chlamydomonas reinhardtii~~]]	+
		+	==Reference==
		+	<ref group="xtra">PMID:024168557</ref><references group="xtra"/><references/>
		+	[[Category: Chlre]]
	[[Category: Small monomeric GTPase]]		[[Category: Small monomeric GTPase]]
	[[Category: Koerner, C.]]		[[Category: Koerner, C.]]

OCA at 07:52, 6 November 2013

2013-11-06T07:52:35Z

←Older revision		Revision as of 07:52, 6 November 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4m9q\| PDB=4m9q \| SCENE= }}
		+	===Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp===

-	~~The entry~~ 4m9q is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4m9q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M9Q OCA].
-	~~Authors~~: ~~Miertzschke, M.,~~ Koerner, C., Spoerner, M., Wittinghofer, A.	+	[[Category: Chlamydomonas reinhardtii]]
-		+	[[Category: Small monomeric GTPase]]
-	~~Description~~: ~~Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp~~	+	[[Category: Koerner, C.]]
		+	[[Category: Miertzschke, M.]]
		+	[[Category: Spoerner, M.]]
		+	[[Category: Wittinghofer, A.]]
		+	[[Category: Cilia]]
		+	[[Category: G domain]]
		+	[[Category: Gtpase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Joubert syndrome]]

OCA at 07:34, 29 September 2013

2013-09-29T07:34:53Z

←Older revision		Revision as of 07:34, 29 September 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4m9q is ON HOLD	+	The entry 4m9q is ON HOLD until Paper Publication

	Authors: Miertzschke, M., Koerner, C., Spoerner, M., Wittinghofer, A.		Authors: Miertzschke, M., Koerner, C., Spoerner, M., Wittinghofer, A.

	Description: Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp		Description: Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp

OCA: Protected "4m9q" [edit=sysop:move=sysop]

2013-08-21T08:00:17Z

Protected "4m9q" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:00, 21 August 2013

OCA: New page: '''Unreleased structure''' The entry 4m9q is ON HOLD Authors: Miertzschke, M., Koerner, C., Spoerner, M., Wittinghofer, A. Description: Crystal structure of C-terminally truncated Arl1...

2013-08-21T08:00:17Z

New page: '''Unreleased structure''' The entry 4m9q is ON HOLD Authors: Miertzschke, M., Koerner, C., Spoerner, M., Wittinghofer, A. Description: Crystal structure of C-terminally truncated Arl1...

New page

'''Unreleased structure'''

The entry 4m9q is ON HOLD

Authors: Miertzschke, M., Koerner, C., Spoerner, M., Wittinghofer, A.

Description: Crystal structure of C-terminally truncated Arl13B from Chlamydomonas rheinhardtii bound to GppNHp