4mdx - Revision history

OCA at 12:26, 1 March 2024

2024-03-01T12:26:08Z

←Older revision		Revision as of 12:26, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4mdx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MDX FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mdx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MDX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mdx OCA], [https://pdbe.org/4mdx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mdx RCSB], [https://www.ebi.ac.uk/pdbsum/4mdx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mdx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mdx OCA], [https://pdbe.org/4mdx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mdx RCSB], [https://www.ebi.ac.uk/pdbsum/4mdx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mdx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ENDOA_BACSU ENDOA_BACSU] Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.<ref>PMID:15882409</ref> <ref>PMID:21763692</ref>		[https://www.uniprot.org/uniprot/ENDOA_BACSU ENDOA_BACSU] Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.<ref>PMID:15882409</ref> <ref>PMID:21763692</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	MazF is an mRNA interferase, which, upon activation during stress conditions, cleaves mRNAs in a sequence-specific manner, resulting in cellular growth arrest. During normal growth conditions, the MazF toxin is inactivated through binding to its cognate antitoxin, MazE. How MazF specifically recognizes its mRNA target and carries out cleavage and how the formation of the MazE-MazF complex inactivates MazF remain unclear. We present crystal structures of MazF in complex with mRNA substrate and antitoxin MazE in Bacillus subtilis. The structure of MazF in complex with uncleavable UUdUACAUAA RNA substrate defines the molecular basis underlying the sequence-specific recognition of UACAU and the role of residues involved in the cleavage through site-specific mutational studies. The structure of the heterohexameric (MazF)2-(MazE)2-(MazF)2 complex in Bacillus subtilis, supplemented by mutational data, demonstrates that the positioning of the C-terminal helical segment of MazE within the RNA-binding channel of the MazF dimer prevents mRNA binding and cleavage by MazF.
-
-	Structural Basis of mRNA Recognition and Cleavage by Toxin MazF and Its Regulation by Antitoxin MazE in Bacillus subtilis.,Simanshu DK, Yamaguchi Y, Park JH, Inouye M, Patel DJ Mol Cell. 2013 Oct 8. pii: S1097-2765(13)00676-X. doi:, 10.1016/j.molcel.2013.09.006. PMID:24120662<ref>PMID:24120662</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4mdx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:11, 21 December 2022

2022-12-21T11:11:28Z

←Older revision		Revision as of 11:11, 21 December 2022
Line 1:		Line 1:

	==Crystal structure of Bacillus subtilis MazF in complex with RNA==		==Crystal structure of Bacillus subtilis MazF in complex with RNA==
-	<StructureSection load='4mdx' size='340' side='right' caption='[[4mdx]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='4mdx' size='340' side='right'caption='[[4mdx]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4mdx]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacsu Bacsu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4MDX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4mdx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MDX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DU:2-DEOXYURIDINE-5-MONOPHOSPHATE'>DU</scene></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mdx OCA], [https://pdbe.org/4mdx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mdx RCSB], [https://www.ebi.ac.uk/pdbsum/4mdx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mdx ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU04660, mazF, ndoA, ydcE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4mdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mdx OCA], [~~http~~://pdbe.org/4mdx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4mdx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4mdx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4mdx ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ENDOA_BACSU ENDOA_BACSU]] Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.<ref>PMID:15882409</ref> <ref>PMID:21763692</ref>	+	[https://www.uniprot.org/uniprot/ENDOA_BACSU ENDOA_BACSU] Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.<ref>PMID:15882409</ref> <ref>PMID:21763692</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacsu~~]]	+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
-	[[Category: ~~Patel, D J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Simanshu, D K~~]]	+	[[Category: Patel DJ]]
-	[[Category: ~~Antitoxin]]~~	+	[[Category: Simanshu DK]]
-	~~[[Category: Endoa]]~~	+
-	~~[[Category: Hydrolase-rna complex]]~~	+
-	~~[[Category: Maze]]~~	+
-	~~[[Category: Mazf]]~~	+
-	~~[[Category: Mrna interferase]]~~	+
-	~~[[Category: Toxin]]~~	+
-	~~[[Category: Toxin-antitoxin complex]]~~	+
-	~~[[Category: Toxin-mrna complex]]~~	+
-	~~[[Category: Ydce~~]]	+

OCA at 15:53, 4 August 2016

2016-08-04T15:53:34Z

←Older revision		Revision as of 15:53, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Bacillus subtilis MazF in complex with RNA==		==Crystal structure of Bacillus subtilis MazF in complex with RNA==
	<StructureSection load='4mdx' size='340' side='right' caption='[[4mdx]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='4mdx' size='340' side='right' caption='[[4mdx]], [[Resolution\|resolution]] 1.50Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DU:2-DEOXYURIDINE-5-MONOPHOSPHATE'>DU</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DU:2-DEOXYURIDINE-5-MONOPHOSPHATE'>DU</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU04660, mazF, ndoA, ydcE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU04660, mazF, ndoA, ydcE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mdx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mdx RCSB], [http://www.ebi.ac.uk/pdbsum/4mdx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mdx OCA], [http://pdbe.org/4mdx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mdx RCSB], [http://www.ebi.ac.uk/pdbsum/4mdx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mdx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4mdx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:45, 25 December 2014

2014-12-25T10:45:05Z

←Older revision		Revision as of 10:45, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4mdx\| PDB=4mdx \| SCENE= }}~~	+	==Crystal structure of Bacillus subtilis MazF in complex with RNA==
-	===Crystal structure of Bacillus subtilis MazF in complex with RNA===	+	<StructureSection load='4mdx' size='340' side='right' caption='[[4mdx]], [[Resolution\|resolution]] 1.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24120662}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4mdx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MDX FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DU:2-DEOXYURIDINE-5-MONOPHOSPHATE'>DU</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU04660, mazF, ndoA, ydcE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mdx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mdx RCSB], [http://www.ebi.ac.uk/pdbsum/4mdx PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/ENDOA_BACSU ENDOA_BACSU]] Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.<ref>PMID:15882409</ref> <ref>PMID:21763692</ref>		[[http://www.uniprot.org/uniprot/ENDOA_BACSU ENDOA_BACSU]] Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.<ref>PMID:15882409</ref> <ref>PMID:21763692</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MazF is an mRNA interferase, which, upon activation during stress conditions, cleaves mRNAs in a sequence-specific manner, resulting in cellular growth arrest. During normal growth conditions, the MazF toxin is inactivated through binding to its cognate antitoxin, MazE. How MazF specifically recognizes its mRNA target and carries out cleavage and how the formation of the MazE-MazF complex inactivates MazF remain unclear. We present crystal structures of MazF in complex with mRNA substrate and antitoxin MazE in Bacillus subtilis. The structure of MazF in complex with uncleavable UUdUACAUAA RNA substrate defines the molecular basis underlying the sequence-specific recognition of UACAU and the role of residues involved in the cleavage through site-specific mutational studies. The structure of the heterohexameric (MazF)2-(MazE)2-(MazF)2 complex in Bacillus subtilis, supplemented by mutational data, demonstrates that the positioning of the C-terminal helical segment of MazE within the RNA-binding channel of the MazF dimer prevents mRNA binding and cleavage by MazF.

-	~~==About this Structure==~~	+	Structural Basis of mRNA Recognition and Cleavage by Toxin MazF and Its Regulation by Antitoxin MazE in Bacillus subtilis.,Simanshu DK, Yamaguchi Y, Park JH, Inouye M, Patel DJ Mol Cell. 2013 Oct 8. pii: S1097-2765(13)00676-X. doi:, 10.1016/j.molcel.2013.09.006. PMID:24120662<ref>PMID:24120662</ref>
-	~~[[4mdx]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacsu Bacsu]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.~~il/oca-bin/ocashort?id=4MDX OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024120662</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bacsu]]		[[Category: Bacsu]]
-	[[Category: Patel, D J.]]	+	[[Category: Patel, D J]]
-	[[Category: Simanshu, D K.]]	+	[[Category: Simanshu, D K]]
	[[Category: Antitoxin]]		[[Category: Antitoxin]]
	[[Category: Endoa]]		[[Category: Endoa]]

OCA at 09:05, 27 November 2013

2013-11-27T09:05:22Z

←Older revision		Revision as of 09:05, 27 November 2013
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[4mdx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDX OCA].	+	[[4mdx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDX OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:024120662</ref><references group="xtra"/><references/>		<ref group="xtra">PMID:024120662</ref><references group="xtra"/><references/>
-	[[Category: ~~Bacillus subtilis subsp. subtilis str. 168~~]]	+	[[Category: Bacsu]]
	[[Category: Patel, D J.]]		[[Category: Patel, D J.]]
	[[Category: Simanshu, D K.]]		[[Category: Simanshu, D K.]]

OCA at 08:27, 30 October 2013

2013-10-30T08:27:29Z

←Older revision		Revision as of 08:27, 30 October 2013
Line 1:		Line 1:
	{{STRUCTURE_4mdx\| PDB=4mdx \| SCENE= }}		{{STRUCTURE_4mdx\| PDB=4mdx \| SCENE= }}
	===Crystal structure of Bacillus subtilis MazF in complex with RNA===		===Crystal structure of Bacillus subtilis MazF in complex with RNA===
		+	{{ABSTRACT_PUBMED_24120662}}

	==Function==		==Function==
Line 9:		Line 10:

	==Reference==		==Reference==
-	<references group="xtra"/><references/>	+	<ref group="xtra">PMID:024120662</ref><references group="xtra"/><references/>
	[[Category: Bacillus subtilis subsp. subtilis str. 168]]		[[Category: Bacillus subtilis subsp. subtilis str. 168]]
	[[Category: Patel, D J.]]		[[Category: Patel, D J.]]

OCA at 06:56, 23 October 2013

2013-10-23T06:56:08Z

←Older revision		Revision as of 06:56, 23 October 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4mdx\| PDB=4mdx \| SCENE= }}
		+	===Crystal structure of Bacillus subtilis MazF in complex with RNA===

-	The ~~entry 4mdx~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/ENDOA_BACSU ENDOA_BACSU]] Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.<ref>PMID:15882409</ref> <ref>PMID:21763692</ref>

-	~~Authors~~: ~~Simanshu, D~~.K.~~, Patel, D~~.J.	+	==About this Structure==
		+	[[4mdx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MDX OCA].

-	~~Description~~: ~~Crystal structure of~~ Bacillus subtilis ~~MazF in~~ complex ~~with RNA~~	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
		+	[[Category: Patel, D J.]]
		+	[[Category: Simanshu, D K.]]
		+	[[Category: Antitoxin]]
		+	[[Category: Endoa]]
		+	[[Category: Hydrolase-rna complex]]
		+	[[Category: Maze]]
		+	[[Category: Mazf]]
		+	[[Category: Mrna interferase]]
		+	[[Category: Toxin]]
		+	[[Category: Toxin-antitoxin complex]]
		+	[[Category: Toxin-mrna complex]]
		+	[[Category: Ydce]]

OCA at 07:23, 16 October 2013

2013-10-16T07:23:56Z

←Older revision		Revision as of 07:23, 16 October 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4mdx is ON HOLD	+	The entry 4mdx is ON HOLD until Paper Publication

	Authors: Simanshu, D.K., Patel, D.J.		Authors: Simanshu, D.K., Patel, D.J.

	Description: Crystal structure of Bacillus subtilis MazF in complex with RNA		Description: Crystal structure of Bacillus subtilis MazF in complex with RNA

OCA: Protected "4mdx" [edit=sysop:move=sysop]

2013-09-04T10:02:35Z

Protected "4mdx" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:02, 4 September 2013

OCA: New page: '''Unreleased structure''' The entry 4mdx is ON HOLD Authors: Simanshu, D.K., Patel, D.J. Description: Crystal structure of Bacillus subtilis MazF in complex with RNA

2013-09-04T10:02:33Z

New page: '''Unreleased structure''' The entry 4mdx is ON HOLD Authors: Simanshu, D.K., Patel, D.J. Description: Crystal structure of Bacillus subtilis MazF in complex with RNA

New page

'''Unreleased structure'''

The entry 4mdx is ON HOLD

Authors: Simanshu, D.K., Patel, D.J.

Description: Crystal structure of Bacillus subtilis MazF in complex with RNA