4mla - Revision history

OCA at 09:47, 28 December 2022

2022-12-28T09:47:12Z

←Older revision		Revision as of 09:47, 28 December 2022
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	<StructureSection load='4mla' size='340' side='right'caption='[[4mla]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='4mla' size='340' side='right'caption='[[4mla]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4mla]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Maize Maize~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MLA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4MLA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4mla]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MLA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ml8\|4ml8]], [[3s1d\|3s1d]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mla OCA], [https://pdbe.org/4mla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mla RCSB], [https://www.ebi.ac.uk/pdbsum/4mla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mla ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cko2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4mla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mla OCA], [~~http~~://pdbe.org/4mla PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4mla RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4mla PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4mla ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q709Q5_MAIZE Q709Q5_MAIZE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Maize~~]]	+	[[Category: Zea mays]]
-	[[Category: Briozzo, P]]	+	[[Category: Briozzo P]]
-	[[Category: Koncitikova, R]]	+	[[Category: Koncitikova R]]
-	[[Category: Kopecny, D]]	+	[[Category: Kopecny D]]
-	[[Category: Morera, S]]	+	[[Category: Morera S]]
-	~~[[Category: Cytokinin binding]]~~	+
-	~~[[Category: Cytokinin oxidase/dehydrogenase]]~~	+
-	~~[[Category: Fad binding protein]]~~	+
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 08:22, 10 April 2019

2019-04-10T08:22:54Z

←Older revision		Revision as of 08:22, 10 April 2019
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	==Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)==		==Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)==
-	<StructureSection load='4mla' size='340' side='right' caption='[[4mla]], [[Resolution\|resolution]] 2.04Å' scene=''>	+	<StructureSection load='4mla' size='340' side='right'caption='[[4mla]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4mla]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MLA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4mla]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MLA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ml8\|4ml8]], [[3s1d\|3s1d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ml8\|4ml8]], [[3s1d\|3s1d]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mla OCA], [http://pdbe.org/4mla PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mla RCSB], [http://www.ebi.ac.uk/pdbsum/4mla PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cko2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mla OCA], [http://pdbe.org/4mla PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mla RCSB], [http://www.ebi.ac.uk/pdbsum/4mla PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mla ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Maize]]
	[[Category: Briozzo, P]]		[[Category: Briozzo, P]]
	[[Category: Koncitikova, R]]		[[Category: Koncitikova, R]]

OCA at 18:07, 10 May 2016

2016-05-10T18:07:07Z

←Older revision		Revision as of 18:07, 10 May 2016
Line 1:		Line 1:
		+
	==Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)==		==Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)==
	<StructureSection load='4mla' size='340' side='right' caption='[[4mla]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='4mla' size='340' side='right' caption='[[4mla]], [[Resolution\|resolution]] 2.04Å' scene=''>
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mla OCA], [http://pdbe.org/4mla PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mla RCSB], [http://www.ebi.ac.uk/pdbsum/4mla PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mla OCA], [http://pdbe.org/4mla PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mla RCSB], [http://www.ebi.ac.uk/pdbsum/4mla PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytokinins are hormones that regulate plant development and their environmental responses. Their levels are mainly controlled by the cytokinin oxidase/dehydrogenase (CKO), which oxidatively cleaves cytokinins using redox-active electron acceptors. CKO belongs to the group of flavoproteins with an 8alpha-N1-histidyl FAD covalent linkage. Here, we investigated the role of seven active site residues: H105, D169, E288, V378, E381, P427 and L492, in substrate binding and catalysis of the CKO1 from maize (Zea mays, ZmCKO1) combining site-directed mutagenesis with kinetics and X-ray crystallography. Here, we identify E381 as a key residue for enzyme specificity that restricts substrate binding as well as quinone electron acceptor binding. We show that D169 is important for catalysis and that H105 covalently linked to FAD maintains the enzyme's structural integrity, stability and high rates with electron acceptors. The L492A mutation significantly modulates the cleavage of aromatic cytokinins and zeatin isomers. The high resolution X-ray structures of ZmCKO1 and the E381S variant in complex with N6-(2-isopentenyl)adenosine (iPR) reveal the binding mode of cytokinin ribosides. Those of ZmCKO2 and ZmCKO4a contain a mobile domain, which might contribute to binding of the N9 substituted cytokinins. This article is protected by copyright. All rights reserved.
		+
		+	Kinetic and structural investigation of the cytokinin oxidase/dehydrogenase active site.,Kopecny D, Koncitikova R, Popelka H, Briozzo P, Vigouroux A, Kopecna M, Zalabak D, Sebela M, Skopalova J, Frebort I, Morera S FEBS J. 2015 Oct 31. doi: 10.1111/febs.13581. PMID:26519657<ref>PMID:26519657</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4mla" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 19:24, 30 November 2015

2015-11-30T19:24:17Z

←Older revision		Revision as of 19:24, 30 November 2015
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ml8\|4ml8]], [[3s1d\|3s1d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ml8\|4ml8]], [[3s1d\|3s1d]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mla OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mla RCSB], [http://www.ebi.ac.uk/pdbsum/4mla PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mla OCA], [http://pdbe.org/4mla PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mla RCSB], [http://www.ebi.ac.uk/pdbsum/4mla PDBsum]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 11:53, 11 March 2015

2015-03-11T11:53:13Z

←Older revision		Revision as of 11:53, 11 March 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)==
-		+	<StructureSection load='4mla' size='340' side='right' caption='[[4mla]], [[Resolution\|resolution]] 2.04Å' scene=''>
-	~~The entry~~ 4mla is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4mla]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MLA FirstGlance]. <br>
-	~~Authors~~: ~~Morera, S~~.~~, Kopecny, D~~.~~, Briozzo, P~~.~~, Koncitikova, R~~.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ml8\|4ml8]], [[3s1d\|3s1d]]</td></tr>
-	~~Description~~: ~~Structure of maize cytokinin oxidase~~/~~dehydrogenase~~ 2 ~~(ZmCKO2)~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mla OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mla RCSB], [http://www.ebi.ac.uk/pdbsum/4mla PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Morera~~, S]]	+	__TOC__
		+	</StructureSection>
	[[Category: Briozzo, P]]		[[Category: Briozzo, P]]
-	[[Category: Kopecny, D]]
	[[Category: Koncitikova, R]]		[[Category: Koncitikova, R]]
		+	[[Category: Kopecny, D]]
		+	[[Category: Morera, S]]
		+	[[Category: Cytokinin binding]]
		+	[[Category: Cytokinin oxidase/dehydrogenase]]
		+	[[Category: Fad binding protein]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Oxidoreductase]]

OCA at 14:05, 24 December 2014

2014-12-24T14:05:43Z

←Older revision		Revision as of 14:05, 24 December 2014
Line 6:		Line 6:

	Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)		Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)
		+	[[Category: Unreleased Structures]]
		+	[[Category: Morera, S]]
		+	[[Category: Briozzo, P]]
		+	[[Category: Kopecny, D]]
		+	[[Category: Koncitikova, R]]

OCA at 06:50, 2 October 2013

2013-10-02T06:50:19Z

←Older revision		Revision as of 06:50, 2 October 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4mla is ON HOLD	+	The entry 4mla is ON HOLD until Paper Publication

	Authors: Morera, S., Kopecny, D., Briozzo, P., Koncitikova, R.		Authors: Morera, S., Kopecny, D., Briozzo, P., Koncitikova, R.

	Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)		Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)

OCA: Protected "4mla" [edit=sysop:move=sysop]

2013-09-20T03:40:39Z

Protected "4mla" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:40, 20 September 2013

OCA: New page: '''Unreleased structure''' The entry 4mla is ON HOLD Authors: Morera, S., Kopecny, D., Briozzo, P., Koncitikova, R. Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (Z...

2013-09-20T03:40:38Z

New page: '''Unreleased structure''' The entry 4mla is ON HOLD Authors: Morera, S., Kopecny, D., Briozzo, P., Koncitikova, R. Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (Z...

New page

'''Unreleased structure'''

The entry 4mla is ON HOLD

Authors: Morera, S., Kopecny, D., Briozzo, P., Koncitikova, R.

Description: Structure of maize cytokinin oxidase/dehydrogenase 2 (ZmCKO2)