4mx1 - Revision history

OCA at 16:44, 20 September 2023

2023-09-20T16:44:12Z

←Older revision		Revision as of 16:44, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4mx1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MX1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MX1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mx1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MX1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MX1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MX:2-AMINO-4-OXO-N-{2-[(PHENYLCARBAMOYL)AMINO]ETHYL}-3,4-DIHYDROPTERIDINE-7-CARBOXAMIDE'>1MX</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.59Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MX:2-AMINO-4-OXO-N-{2-[(PHENYLCARBAMOYL)AMINO]ETHYL}-3,4-DIHYDROPTERIDINE-7-CARBOXAMIDE'>1MX</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mx1 OCA], [https://pdbe.org/4mx1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mx1 RCSB], [https://www.ebi.ac.uk/pdbsum/4mx1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mx1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mx1 OCA], [https://pdbe.org/4mx1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mx1 RCSB], [https://www.ebi.ac.uk/pdbsum/4mx1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mx1 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:16, 28 December 2022

2022-12-28T10:16:12Z

←Older revision		Revision as of 10:16, 28 December 2022
Line 3:		Line 3:
	<StructureSection load='4mx1' size='340' side='right'caption='[[4mx1]], [[Resolution\|resolution]] 1.59Å' scene=''>		<StructureSection load='4mx1' size='340' side='right'caption='[[4mx1]], [[Resolution\|resolution]] 1.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4mx1]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Castor_bean Castor bean~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MX1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4MX1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4mx1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MX1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MX1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1MX:2-AMINO-4-OXO-N-{2-[(PHENYLCARBAMOYL)AMINO]ETHYL}-3,4-DIHYDROPTERIDINE-7-CARBOXAMIDE'>1MX</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MX:2-AMINO-4-OXO-N-{2-[(PHENYLCARBAMOYL)AMINO]ETHYL}-3,4-DIHYDROPTERIDINE-7-CARBOXAMIDE'>1MX</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtc\|1rtc]], [[1ift\|1ift]], [[1br6\|1br6]], [[3px8\|3px8]], [[4hup\|4hup]], [[4hv3\|4hv3]], [[4mx5\|4mx5]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mx1 OCA], [https://pdbe.org/4mx1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mx1 RCSB], [https://www.ebi.ac.uk/pdbsum/4mx1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mx1 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4mx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mx1 OCA], [~~http~~://pdbe.org/4mx1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4mx1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4mx1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4mx1 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RICI_RICCO RICI_RICCO]] Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity).	+	[https://www.uniprot.org/uniprot/RICI_RICCO RICI_RICCO] Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[Ricin\|Ricin]]	+	*[[Ricin 3D structures\|Ricin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Castor bean]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~RRNA N-glycosylase~~]]	+	[[Category: Ricinus communis]]
-	[[Category: Anslyn~~, E V~~]]	+	[[Category: Anslyn EV]]
-	[[Category: Gao, G]]	+	[[Category: Gao G]]
-	[[Category: Jasheway~~, K R~~]]	+	[[Category: Jasheway KR]]
-	[[Category: Manzano~~, L A~~]]	+	[[Category: Manzano LA]]
-	[[Category: Monzingo~~, A F~~]]	+	[[Category: Monzingo AF]]
-	[[Category: Pruet~~, J M~~]]	+	[[Category: Pruet JM]]
-	[[Category: Robertus~~, J D~~]]	+	[[Category: Robertus JD]]
-	[[Category: Saito, R]]	+	[[Category: Saito R]]
-	[[Category: Wiget~~, P A]]~~	+	[[Category: Wiget PA]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: N-glycosidase]]~~	+
-	~~[[Category: Pterin]]~~	+
-	~~[[Category: Ribosome-inactivating protein]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 05:25, 13 February 2020

2020-02-13T05:25:12Z

←Older revision		Revision as of 05:25, 13 February 2020
Line 1:		Line 1:

	==Structure of ricin A chain bound with 2-amino-4-oxo-N-(2-(3-phenylureido)ethyl)-3,4-dihydropteridine-7-carboxamide==		==Structure of ricin A chain bound with 2-amino-4-oxo-N-(2-(3-phenylureido)ethyl)-3,4-dihydropteridine-7-carboxamide==
-	<StructureSection load='4mx1' size='340' side='right' caption='[[4mx1]], [[Resolution\|resolution]] 1.59Å' scene=''>	+	<StructureSection load='4mx1' size='340' side='right'caption='[[4mx1]], [[Resolution\|resolution]] 1.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4mx1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Castor_bean Castor bean]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MX1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mx1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Castor_bean Castor bean]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MX1 FirstGlance]. <br>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Castor bean]]		[[Category: Castor bean]]
		+	[[Category: Large Structures]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
	[[Category: Anslyn, E V]]		[[Category: Anslyn, E V]]

OCA at 09:58, 15 November 2017

2017-11-15T09:58:48Z

←Older revision		Revision as of 09:58, 15 November 2017
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4mx1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4mx1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ricin\|Ricin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:44, 1 February 2017

2017-02-01T18:44:05Z

←Older revision		Revision as of 18:44, 1 February 2017
Line 1:		Line 1:
		+
	==Structure of ricin A chain bound with 2-amino-4-oxo-N-(2-(3-phenylureido)ethyl)-3,4-dihydropteridine-7-carboxamide==		==Structure of ricin A chain bound with 2-amino-4-oxo-N-(2-(3-phenylureido)ethyl)-3,4-dihydropteridine-7-carboxamide==
	<StructureSection load='4mx1' size='340' side='right' caption='[[4mx1]], [[Resolution\|resolution]] 1.59Å' scene=''>		<StructureSection load='4mx1' size='340' side='right' caption='[[4mx1]], [[Resolution\|resolution]] 1.59Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtc\|1rtc]], [[1ift\|1ift]], [[1br6\|1br6]], [[3px8\|3px8]], [[4hup\|4hup]], [[4hv3\|4hv3]], [[4mx5\|4mx5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtc\|1rtc]], [[1ift\|1ift]], [[1br6\|1br6]], [[3px8\|3px8]], [[4hup\|4hup]], [[4hv3\|4hv3]], [[4mx5\|4mx5]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mx1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mx1 RCSB], [http://www.ebi.ac.uk/pdbsum/4mx1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mx1 OCA], [http://pdbe.org/4mx1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mx1 RCSB], [http://www.ebi.ac.uk/pdbsum/4mx1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mx1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
-		+	<div class="pdbe-citations 4mx1" style="background-color:#fffaf0;"></div>
-	==~~See Also==~~	+
-	*[[Ricin\|Ricin]]	+
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:06, 25 December 2014

2014-12-25T05:06:18Z

←Older revision		Revision as of 05:06, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mx1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mx1 RCSB], [http://www.ebi.ac.uk/pdbsum/4mx1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mx1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mx1 RCSB], [http://www.ebi.ac.uk/pdbsum/4mx1 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RICI_RICCO RICI_RICCO]] Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:20, 21 December 2014

2014-12-21T17:20:43Z

←Older revision		Revision as of 17:20, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4mx1\| PDB=4mx1 \| SCENE= }}~~	+	==Structure of ricin A chain bound with 2-amino-4-oxo-N-(2-(3-phenylureido)ethyl)-3,4-dihydropteridine-7-carboxamide==
-	===Structure of ricin A chain bound with 2-amino-4-oxo-N-(2-(3-phenylureido)ethyl)-3,4-dihydropteridine-7-carboxamide===	+	<StructureSection load='4mx1' size='340' side='right' caption='[[4mx1]], [[Resolution\|resolution]] 1.59Å' scene=''>
-	{{~~ABSTRACT_PUBMED_24432385}~~}	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4mx1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Castor_bean Castor bean]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MX1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MX1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1MX:2-AMINO-4-OXO-N-{2-[(PHENYLCARBAMOYL)AMINO]ETHYL}-3,4-DIHYDROPTERIDINE-7-CARBOXAMIDE'>1MX</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtc\|1rtc]], [[1ift\|1ift]], [[1br6\|1br6]], [[3px8\|3px8]], [[4hup\|4hup]], [[4hv3\|4hv3]], [[4mx5\|4mx5]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mx1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mx1 RCSB], [http://www.ebi.ac.uk/pdbsum/4mx1 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Several 7-aminoamido-pterins were synthesized to evaluate the electronic and biochemical subtleties observed in the 'linker space' when N-{N-(pterin-7-yl)carbonylglycyl}-l-phenylalanine 1 was bound to the active site of RTA. The gylcine-phenylalanine dipeptide analogs included both amides and thioamides. Decarboxy gly-phe analog 2 showed a 6.4-fold decrease in potency (IC50 = 128 muM), yet the analogous thioamide 7 recovered the lost activity and performed similarly to the parent inhibitor (IC50 = 29 muM). Thiourea 12 exhibited an IC50 nearly six times lower than the oxo analog 13. All inhibitors showed the pterin head-group firmly bound in their X-ray structures yet the pendants were not fully resolved suggesting that all pendants are not firmly bound in the RTA linker space. Calculated log P values do not correlate to the increase in bioactivity suggesting other factors dominate.

-	~~==Function==~~	+	Sulfur incorporation generally improves Ricin inhibition in pterin-appended glycine-phenylalanine dipeptide mimics.,Wiget PA, Manzano LA, Pruet JM, Gao G, Saito R, Monzingo AF, Jasheway KR, Robertus JD, Anslyn EV Bioorg Med Chem Lett. 2013 Dec 15;23(24):6799-804. PMID:24432385<ref>PMID:24432385</ref>
-	~~[[http://www.uniprot.org/uniprot/RICI_RICCO RICI_RICCO]]~~ Ricin ~~is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324~~ in ~~mammals), leading to rRNA breakage~~. ~~As this loop is involved in elongation factor binding~~, ~~modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute~~, ~~faster than the cell can make new ones~~. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; ~~B chains are also responsible for cell agglutination~~ (~~Lectin activity~~).	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4mx1]] is~~ a ~~1 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4MX1 OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:024432385</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Ricin\|Ricin]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Castor bean]]
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
-	[[Category: Anslyn, E V.]]	+	[[Category: Anslyn, E V]]
-	[[Category: Gao, G.]]	+	[[Category: Gao, G]]
-	[[Category: Jasheway, K R.]]	+	[[Category: Jasheway, K R]]
-	[[Category: Manzano, L A.]]	+	[[Category: Manzano, L A]]
-	[[Category: Monzingo, A F.]]	+	[[Category: Monzingo, A F]]
-	[[Category: Pruet, J M.]]	+	[[Category: Pruet, J M]]
-	[[Category: Robertus, J D.]]	+	[[Category: Robertus, J D]]
-	[[Category: Saito, R.]]	+	[[Category: Saito, R]]
-	[[Category: Wiget, P A.]]	+	[[Category: Wiget, P A]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]

OCA at 04:09, 13 February 2014

2014-02-13T04:09:10Z

←Older revision		Revision as of 04:09, 13 February 2014
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	{{STRUCTURE_4mx1\| PDB=4mx1 \| SCENE= }}		{{STRUCTURE_4mx1\| PDB=4mx1 \| SCENE= }}
	===Structure of ricin A chain bound with 2-amino-4-oxo-N-(2-(3-phenylureido)ethyl)-3,4-dihydropteridine-7-carboxamide===		===Structure of ricin A chain bound with 2-amino-4-oxo-N-(2-(3-phenylureido)ethyl)-3,4-dihydropteridine-7-carboxamide===
		+	{{ABSTRACT_PUBMED_24432385}}

	==Function==		==Function==
Line 7:		Line 8:
	==About this Structure==		==About this Structure==
	[[4mx1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MX1 OCA].		[[4mx1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MX1 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024432385</ref><references group="xtra"/><references/>
	[[Category: RRNA N-glycosylase]]		[[Category: RRNA N-glycosylase]]
	[[Category: Anslyn, E V.]]		[[Category: Anslyn, E V.]]

OCA at 08:25, 15 January 2014

2014-01-15T08:25:56Z

←Older revision		Revision as of 08:25, 15 January 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4mx1\| PDB=4mx1 \| SCENE= }}
		+	===Structure of ricin A chain bound with 2-amino-4-oxo-N-(2-(3-phenylureido)ethyl)-3,4-dihydropteridine-7-carboxamide===

-	The ~~entry 4mx1~~ is ~~ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/RICI_RICCO RICI_RICCO]] Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity).

-	~~Authors~~: ~~Robertus, J~~.D., ~~Wiget~~, ~~P.A~~., Manzano, L.A., Pruet, J.M., ~~Gao, G~~., Saito, R., ~~Jasheway, K.R., Monzingo,~~ A.~~F., Anslyn, E.V.~~	+	==About this Structure==
-		+	[[4mx1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MX1 OCA].
-	~~Description~~: ~~Structure of ricin A chain bound with 2-amino-4-oxo~~-N-(2-~~(3-phenylureido)ethyl)-3,4-dihydropteridine-7-carboxamide~~	+	[[Category: RRNA N-glycosylase]]
		+	[[Category: Anslyn, E V.]]
		+	[[Category: Gao, G.]]
		+	[[Category: Jasheway, K R.]]
		+	[[Category: Manzano, L A.]]
		+	[[Category: Monzingo, A F.]]
		+	[[Category: Pruet, J M.]]
		+	[[Category: Robertus, J D.]]
		+	[[Category: Saito, R.]]
		+	[[Category: Wiget, P A.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase-hydrolase inhibitor complex]]
		+	[[Category: N-glycosidase]]
		+	[[Category: Pterin]]
		+	[[Category: Ribosome-inactivating protein]]
		+	[[Category: Toxin]]

OCA at 12:53, 20 November 2013

2013-11-20T12:53:56Z

←Older revision		Revision as of 12:53, 20 November 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4mx1 is ON HOLD	+	The entry 4mx1 is ON HOLD until Paper Publication

	Authors: Robertus, J.D., Wiget, P.A., Manzano, L.A., Pruet, J.M., Gao, G., Saito, R., Jasheway, K.R., Monzingo, A.F., Anslyn, E.V.		Authors: Robertus, J.D., Wiget, P.A., Manzano, L.A., Pruet, J.M., Gao, G., Saito, R., Jasheway, K.R., Monzingo, A.F., Anslyn, E.V.

	Description: Structure of ricin A chain bound with 2-amino-4-oxo-N-(2-(3-phenylureido)ethyl)-3,4-dihydropteridine-7-carboxamide		Description: Structure of ricin A chain bound with 2-amino-4-oxo-N-(2-(3-phenylureido)ethyl)-3,4-dihydropteridine-7-carboxamide