4mxr - Revision history

OCA at 16:44, 20 September 2023

2023-09-20T16:44:25Z

←Older revision		Revision as of 16:44, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4mxr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MXR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MXR FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mxr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MXR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MXR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.849Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mxr OCA], [https://pdbe.org/4mxr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mxr RCSB], [https://www.ebi.ac.uk/pdbsum/4mxr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mxr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mxr OCA], [https://pdbe.org/4mxr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mxr RCSB], [https://www.ebi.ac.uk/pdbsum/4mxr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mxr ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:17, 28 December 2022

2022-12-28T10:17:40Z

←Older revision		Revision as of 10:17, 28 December 2022
Line 3:		Line 3:
	<StructureSection load='4mxr' size='340' side='right'caption='[[4mxr]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='4mxr' size='340' side='right'caption='[[4mxr]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4mxr]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Trycr Trycr~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MXR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4MXR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4mxr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MXR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MXR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a0m\|2a0m]], [[4myf\|4myf]], [[4myk\|4myk]], [[4myl\|4myl]], [[4myn\|4myn]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mxr OCA], [https://pdbe.org/4mxr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mxr RCSB], [https://www.ebi.ac.uk/pdbsum/4mxr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mxr ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formimidoylglutamase Formimidoylglutamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.8 3.5.3.8] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4mxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mxr OCA], [~~http~~://pdbe.org/4mxr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4mxr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4mxr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4mxr ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q4DSA0_TRYCC Q4DSA0_TRYCC]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Formimidoylglutamase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Trycr~~]]	+	[[Category: Trypanosoma cruzi]]
-	[[Category: Christianson~~, D W~~]]	+	[[Category: Christianson DW]]
-	[[Category: Dugery, R J]]	+	[[Category: Dugery R-J]]
-	[[Category: Hai, Y]]	+	[[Category: Hai Y]]
-	[[Category: Healy, D]]	+	[[Category: Healy D]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 08:22, 10 April 2019

2019-04-10T08:22:29Z

←Older revision		Revision as of 08:22, 10 April 2019
Line 1:		Line 1:

	==Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2==		==Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2==
-	<StructureSection load='4mxr' size='340' side='right' caption='[[4mxr]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='4mxr' size='340' side='right'caption='[[4mxr]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4mxr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MXR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MXR FirstGlance]. <br>		<table><tr><td colspan='2'>[[4mxr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MXR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MXR FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Formimidoylglutamase]]		[[Category: Formimidoylglutamase]]
		+	[[Category: Large Structures]]
	[[Category: Trycr]]		[[Category: Trycr]]
	[[Category: Christianson, D W]]		[[Category: Christianson, D W]]

OCA at 18:50, 11 August 2016

2016-08-11T18:50:50Z

←Older revision		Revision as of 18:50, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2==		==Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2==
	<StructureSection load='4mxr' size='340' side='right' caption='[[4mxr]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='4mxr' size='340' side='right' caption='[[4mxr]], [[Resolution\|resolution]] 1.85Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a0m\|2a0m]], [[4myf\|4myf]], [[4myk\|4myk]], [[4myl\|4myl]], [[4myn\|4myn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a0m\|2a0m]], [[4myf\|4myf]], [[4myk\|4myk]], [[4myl\|4myl]], [[4myn\|4myn]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formimidoylglutamase Formimidoylglutamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.8 3.5.3.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formimidoylglutamase Formimidoylglutamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.8 3.5.3.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mxr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mxr RCSB], [http://www.ebi.ac.uk/pdbsum/4mxr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mxr OCA], [http://pdbe.org/4mxr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mxr RCSB], [http://www.ebi.ac.uk/pdbsum/4mxr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mxr ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4mxr" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:35, 5 January 2015

2015-01-05T12:35:23Z

←Older revision		Revision as of 12:35, 5 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4mxr~~\| ~~PDB~~=4mxr \| ~~SCENE~~= }}	+	==Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2==
-	===~~Crystal~~ structure of Trypanosoma cruzi formiminoglutamase with ~~Mn2~~+2~~===~~	+	<StructureSection load='4mxr' size='340' side='right' caption='[[4mxr]], [[Resolution\|resolution]] 1.85Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24261485}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4mxr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MXR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MXR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a0m\|2a0m]], [[4myf\|4myf]], [[4myk\|4myk]], [[4myl\|4myl]], [[4myn\|4myn]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formimidoylglutamase Formimidoylglutamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.8 3.5.3.8] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mxr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mxr RCSB], [http://www.ebi.ac.uk/pdbsum/4mxr PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of formiminoglutamase from Trypanosoma cruzi (TcFIGase) is reported at 1.85 A resolution. Although the structure of this enzyme was previously determined by the Structural Genomics of Pathogenic Protozoa Consortium (PDB accession code 2A0M), this structure was determined at low pH and lacked bound metal ions; accordingly, the protein was simply annotated as "arginase superfamily protein" with undetermined function. We show that reconstitution of this protein with Mn(2+) confers maximal catalytic activity in the hydrolysis of formiminoglutamate to yield glutamate and formamide, thereby demonstrating that this protein is a metal-dependent formiminoglutamase. Equilibration of TcFIGase crystals with MnCl2 at higher pH yields a binuclear manganese cluster similar to that observed in arginase, except that the histidine ligand to the Mn(2+)A ion of arginase is an asparagine ligand (N114) to the Mn(2+)A ion of TcFIGase. The crystal structure of N114H TcFIGase reveals a binuclear manganese cluster essentially identical to that of arginase, but the mutant exhibits a modest 35% loss of catalytic efficiency (kcat/KM). Interestingly, when TcFIGase is prepared and crystallized in the absence of reducing agents at low pH, a disulfide linkage forms between C35 and C242 in the active site. When reconstituted with Mn(2+) at higher pH, this oxidized enzyme exhibits a modest 33% loss of catalytic efficiency. Structure determinations of the metal-free and metal-bound forms of oxidized TcFIGase reveal that although disulfide formation constricts the main entrance to the active site, other structural changes open alternative channels to the active site that may help sustain catalytic activity.

-	~~==About this Structure==~~	+	Formiminoglutamase from trypanosoma cruzi is an arginase-like manganese metalloenzyme.,Hai Y, Dugery RJ, Healy D, Christianson DW Biochemistry. 2013 Dec 23;52(51):9294-309. doi: 10.1021/bi401352h. Epub 2013 Nov , 21. PMID:24261485<ref>PMID:24261485</ref>
-	~~[[4mxr]]~~ is ~~a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Trycr Trycr]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4MXR OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024261485</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Formimidoylglutamase]]		[[Category: Formimidoylglutamase]]
	[[Category: Trycr]]		[[Category: Trycr]]
-	[[Category: Christianson, D W.]]	+	[[Category: Christianson, D W]]
-	[[Category: Dugery, R J.]]	+	[[Category: Dugery, R J]]
-	[[Category: Hai, Y.]]	+	[[Category: Hai, Y]]
-	[[Category: Healy, D.]]	+	[[Category: Healy, D]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 08:38, 5 February 2014

2014-02-05T08:38:39Z

←Older revision		Revision as of 08:38, 5 February 2014
Line 1:		Line 1:
	{{STRUCTURE_4mxr\| PDB=4mxr \| SCENE= }}		{{STRUCTURE_4mxr\| PDB=4mxr \| SCENE= }}
	===Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2===		===Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2===
		+	{{ABSTRACT_PUBMED_24261485}}

	==About this Structure==		==About this Structure==
-	[[4mxr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MXR OCA].	+	[[4mxr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trycr Trycr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MXR OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024261485</ref><references group="xtra"/><references/>
	[[Category: Formimidoylglutamase]]		[[Category: Formimidoylglutamase]]
		+	[[Category: Trycr]]
	[[Category: Christianson, D W.]]		[[Category: Christianson, D W.]]
	[[Category: Dugery, R J.]]		[[Category: Dugery, R J.]]

OCA at 10:36, 27 November 2013

2013-11-27T10:36:21Z

←Older revision		Revision as of 10:36, 27 November 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4mxr\| PDB=4mxr \| SCENE= }}
		+	===Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2===

-	~~The entry~~ 4mxr is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4mxr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MXR OCA].
-	~~Authors~~: ~~Hai~~, Y., Dugery, R.-J., Healy, D.~~, Christianson, D.W.~~	+	[[Category: Formimidoylglutamase]]
-		+	[[Category: Christianson, D W.]]
-	~~Description~~: ~~Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2~~	+	[[Category: Dugery, R J.]]
		+	[[Category: Hai, Y.]]
		+	[[Category: Healy, D.]]
		+	[[Category: Hydrolase]]

OCA at 07:37, 13 November 2013

2013-11-13T07:37:41Z

←Older revision		Revision as of 07:37, 13 November 2013
Line 3:		Line 3:
	The entry 4mxr is ON HOLD		The entry 4mxr is ON HOLD

-	Authors: Hai, Y., Dugery, R-J., Healy, D., Christianson, D.W.	+	Authors: Hai, Y., Dugery, R.-J., Healy, D., Christianson, D.W.

	Description: Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2		Description: Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2

OCA: Protected "4mxr" [edit=sysop:move=sysop]

2013-10-02T06:59:07Z

Protected "4mxr" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:59, 2 October 2013

OCA: New page: '''Unreleased structure''' The entry 4mxr is ON HOLD Authors: Hai, Y., Dugery, R-J., Healy, D., Christianson, D.W. Description: Crystal structure of Trypanosoma cruzi formiminoglutamas...

2013-10-02T06:59:06Z

New page: '''Unreleased structure''' The entry 4mxr is ON HOLD Authors: Hai, Y., Dugery, R-J., Healy, D., Christianson, D.W. Description: Crystal structure of Trypanosoma cruzi formiminoglutamas...

New page

'''Unreleased structure'''

The entry 4mxr is ON HOLD

Authors: Hai, Y., Dugery, R-J., Healy, D., Christianson, D.W.

Description: Crystal structure of Trypanosoma cruzi formiminoglutamase with Mn2+2