4n48 - Revision history

OCA at 16:47, 20 September 2023

2023-09-20T16:47:32Z

←Older revision		Revision as of 16:47, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4n48]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N48 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4n48]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N48 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MGT:7N-METHYL-8-HYDROGUANOSINE-5-TRIPHOSPHATE'>MGT</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.704Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MGT:7N-METHYL-8-HYDROGUANOSINE-5-TRIPHOSPHATE'>MGT</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n48 OCA], [https://pdbe.org/4n48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n48 RCSB], [https://www.ebi.ac.uk/pdbsum/4n48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n48 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n48 OCA], [https://pdbe.org/4n48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n48 RCSB], [https://www.ebi.ac.uk/pdbsum/4n48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n48 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:31, 28 December 2022

2022-12-28T10:31:50Z

←Older revision		Revision as of 10:31, 28 December 2022
Line 1:		Line 1:

	==Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Protein in complex with capped RNA fragment==		==Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Protein in complex with capped RNA fragment==
-	<StructureSection load='4n48' size='340' side='right' caption='[[4n48]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='4n48' size='340' side='right'caption='[[4n48]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n48]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N48 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4N48 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4n48]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N48 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N48 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MGT:7N-METHYL-8-HYDROGUANOSINE-5-TRIPHOSPHATE'>MGT</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MGT:7N-METHYL-8-HYDROGUANOSINE-5-TRIPHOSPHATE'>MGT</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n49\|4n49]], [[4n4a\|4n4a]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n48 OCA], [https://pdbe.org/4n48 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n48 RCSB], [https://www.ebi.ac.uk/pdbsum/4n48 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n48 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTSJD2, KIAA0082, MTR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(nucleoside-2'-O)-methyltransferase mRNA (nucleoside-2'-O)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.57 2.1.1.57] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4n48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n48 OCA], [~~http~~://pdbe.org/4n48 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4n48 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4n48 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4n48 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CMTR1_HUMAN CMTR1_HUMAN] S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap1 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the first nucleotide of a m(7)GpppG-capped mRNA and small nuclear RNA (snRNA) to produce m(7)GpppRm (cap1). Displays a preference for cap0 transcripts. Cap1 modification is linked to higher levels of translation. May be involved in the interferon response pathway.<ref>PMID:18533109</ref> <ref>PMID:20713356</ref> <ref>PMID:21310715</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: Bujnicki~~, J M~~]]	+	[[Category: Large Structures]]
-	[[Category: Darzynkiewicz, E]]	+	[[Category: Bujnicki JM]]
-	[[Category: Kaminska~~, K H~~]]	+	[[Category: Darzynkiewicz E]]
-	[[Category: Nowotny, M]]	+	[[Category: Kaminska KH]]
-	[[Category: Purta, E]]	+	[[Category: Nowotny M]]
-	[[Category: Smietanski, M]]	+	[[Category: Purta E]]
-	[[Category: Stepinski, J]]	+	[[Category: Smietanski M]]
-	[[Category: Werener, M]]	+	[[Category: Stepinski J]]
-	~~[[Category: Capped mrna]]~~	+	[[Category: Werener M]]
-	~~[[Category: Methyltransferase]]~~	+
-	~~[[Category: Mrna cap methylation]]~~	+
-	~~[[Category: Transferase-rna complex~~]]	+

OCA at 09:10, 11 August 2016

2016-08-11T09:10:07Z

←Older revision		Revision as of 09:10, 11 August 2016
Line 1:		Line 1:
		+
	==Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Protein in complex with capped RNA fragment==		==Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Protein in complex with capped RNA fragment==
	<StructureSection load='4n48' size='340' side='right' caption='[[4n48]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4n48' size='340' side='right' caption='[[4n48]], [[Resolution\|resolution]] 2.70Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTSJD2, KIAA0082, MTR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTSJD2, KIAA0082, MTR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(nucleoside-2'-O)-methyltransferase mRNA (nucleoside-2'-O)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.57 2.1.1.57] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(nucleoside-2'-O)-methyltransferase mRNA (nucleoside-2'-O)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.57 2.1.1.57] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n48 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n48 RCSB], [http://www.ebi.ac.uk/pdbsum/4n48 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n48 OCA], [http://pdbe.org/4n48 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n48 RCSB], [http://www.ebi.ac.uk/pdbsum/4n48 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n48 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4n48" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:18, 25 January 2015

2015-01-25T10:18:31Z

←Older revision		Revision as of 10:18, 25 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4n48\| PDB=4n48 \| SCENE= }}~~	+	==Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Protein in complex with capped RNA fragment==
-	===Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Protein in complex with capped RNA fragment===	+	<StructureSection load='4n48' size='340' side='right' caption='[[4n48]], [[Resolution\|resolution]] 2.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24402442}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4n48]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N48 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N48 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MGT:7N-METHYL-8-HYDROGUANOSINE-5-TRIPHOSPHATE'>MGT</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n49\|4n49]], [[4n4a\|4n4a]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FTSJD2, KIAA0082, MTR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(nucleoside-2'-O)-methyltransferase mRNA (nucleoside-2'-O)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.57 2.1.1.57] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n48 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n48 RCSB], [http://www.ebi.ac.uk/pdbsum/4n48 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The 5' cap of human messenger RNA contains 2'-O-methylation of the first and often second transcribed nucleotide that is important for its processing, translation and stability. Human enzymes that methylate these nucleotides, termed CMTr1 and CMTr2, respectively, have recently been identified. However, the structures of these enzymes and their mechanisms of action remain unknown. In the present study, we solve the crystal structures of the active CMTr1 catalytic domain in complex with a methyl group donor and a capped oligoribonucleotide, thereby revealing the mechanism of specific recognition of capped RNA. This mechanism differs significantly from viral enzymes, thus providing a framework for their specific targeting. Based on the crystal structure of CMTr1, a comparative model of the CMTr2 catalytic domain is generated. This model, together with mutational analysis, leads to the identification of residues involved in RNA and methyl group donor binding.

-	~~==About this Structure==~~	+	Structural analysis of human 2'-O-ribose methyltransferases involved in mRNA cap structure formation.,Smietanski M, Werner M, Purta E, Kaminska KH, Stepinski J, Darzynkiewicz E, Nowotny M, Bujnicki JM Nat Commun. 2014 Jan 9;5:3004. doi: 10.1038/ncomms4004. PMID:24402442<ref>PMID:24402442</ref>
-	~~[[4n48]] is a 4 chain~~ structure. ~~Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann.ac~~.il/~~oca-bin/ocashort?id=4N48 OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024402442</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
	[[Category: Bujnicki, J M]]		[[Category: Bujnicki, J M]]
-	[[Category: Darzynkiewicz, E.]]	+	[[Category: Darzynkiewicz, E]]
-	[[Category: Kaminska, K H.]]	+	[[Category: Kaminska, K H]]
-	[[Category: Nowotny, M.]]	+	[[Category: Nowotny, M]]
-	[[Category: Purta, E.]]	+	[[Category: Purta, E]]
-	[[Category: Smietanski, M.]]	+	[[Category: Smietanski, M]]
-	[[Category: Stepinski, J.]]	+	[[Category: Stepinski, J]]
-	[[Category: Werener, M.]]	+	[[Category: Werener, M]]
	[[Category: Capped mrna]]		[[Category: Capped mrna]]
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Mrna cap methylation]]		[[Category: Mrna cap methylation]]
	[[Category: Transferase-rna complex]]		[[Category: Transferase-rna complex]]

OCA at 06:54, 22 January 2014

2014-01-22T06:54:05Z

←Older revision		Revision as of 06:54, 22 January 2014
Line 1:		Line 1:
-	'~~''Unreleased structure'''~~	+	{{STRUCTURE_4n48\| PDB=4n48 \| SCENE= }}
		+	===Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 Protein in complex with capped RNA fragment===
		+	{{ABSTRACT_PUBMED_24402442}}

-	~~The entry~~ 4n48 is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4n48]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N48 OCA].

-	~~Authors~~: ~~Smietanski~~, M~~., Werener, M., Purta~~, E., Kaminska, K.H., ~~Stepinski, J~~.~~, Darzynkiewicz~~, E.~~, Nowotny~~, M.~~, Bujnicki~~, J.M	+	==Reference==
-		+	<ref group="xtra">PMID:024402442</ref><references group="xtra"/><references/>
-	~~Description~~:	+	[[Category: Bujnicki, J M]]
		+	[[Category: Darzynkiewicz, E.]]
		+	[[Category: Kaminska, K H.]]
		+	[[Category: Nowotny, M.]]
		+	[[Category: Purta, E.]]
		+	[[Category: Smietanski, M.]]
		+	[[Category: Stepinski, J.]]
		+	[[Category: Werener, M.]]
		+	[[Category: Capped mrna]]
		+	[[Category: Methyltransferase]]
		+	[[Category: Mrna cap methylation]]
		+	[[Category: Transferase-rna complex]]

OCA at 09:12, 18 December 2013

2013-12-18T09:12:35Z

←Older revision		Revision as of 09:12, 18 December 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4n48 is ON HOLD	+	The entry 4n48 is ON HOLD until Paper Publication

	Authors: Smietanski, M., Werener, M., Purta, E., Kaminska, K.H., Stepinski, J., Darzynkiewicz, E., Nowotny, M., Bujnicki, J.M		Authors: Smietanski, M., Werener, M., Purta, E., Kaminska, K.H., Stepinski, J., Darzynkiewicz, E., Nowotny, M., Bujnicki, J.M

	Description:		Description:

OCA at 06:46, 4 December 2013

2013-12-04T06:46:48Z

←Older revision		Revision as of 06:46, 4 December 2013
Line 5:		Line 5:
	Authors: Smietanski, M., Werener, M., Purta, E., Kaminska, K.H., Stepinski, J., Darzynkiewicz, E., Nowotny, M., Bujnicki, J.M		Authors: Smietanski, M., Werener, M., Purta, E., Kaminska, K.H., Stepinski, J., Darzynkiewicz, E., Nowotny, M., Bujnicki, J.M

-	Description: ~~Protein-long RNA complex~~	+	Description:

OCA: Protected "4n48" [edit=sysop:move=sysop]

2013-10-16T07:34:28Z

Protected "4n48" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:34, 16 October 2013

OCA: New page: '''Unreleased structure''' The entry 4n48 is ON HOLD Authors: Smietanski, M., Werener, M., Purta, E., Kaminska, K.H., Stepinski, J., Darzynkiewicz, E., Nowotny, M., Bujnicki, J.M Descr...

2013-10-16T07:34:27Z

New page: '''Unreleased structure''' The entry 4n48 is ON HOLD Authors: Smietanski, M., Werener, M., Purta, E., Kaminska, K.H., Stepinski, J., Darzynkiewicz, E., Nowotny, M., Bujnicki, J.M Descr...

New page

'''Unreleased structure'''

The entry 4n48 is ON HOLD

Authors: Smietanski, M., Werener, M., Purta, E., Kaminska, K.H., Stepinski, J., Darzynkiewicz, E., Nowotny, M., Bujnicki, J.M

Description: Protein-long RNA complex