4n9l - Revision history

OCA at 12:23, 15 November 2023

2023-11-15T12:23:29Z

←Older revision		Revision as of 12:23, 15 November 2023
Line 3:		Line 3:
	<StructureSection load='4n9l' size='340' side='right'caption='[[4n9l]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4n9l' size='340' side='right'caption='[[4n9l]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n9l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"denitrobacillus_licheniformis"_(weigmann_1898)_verhoeven_1952 "denitrobacillus~~ licheniformis~~" (weigmann 1898) verhoeven 1952~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N9L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4n9l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N9L FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DWZ:(2S,3R,4S)-4-{[(3S,5S)-5-(DIMETHYLCARBAMOYL)PYRROLIDIN-3-YL]SULFANYL}-2-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-3-METHYL-3,4-DIHYDRO-2H-PYRROLE-5-CARBOXYLIC+ACID'>DWZ</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4n92\|4n92]], [[4n9k\|4n9k]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DWZ:(2S,3R,4S)-4-{[(3S,5S)-5-(DIMETHYLCARBAMOYL)PYRROLIDIN-3-YL]SULFANYL}-2-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-3-METHYL-3,4-DIHYDRO-2H-PYRROLE-5-CARBOXYLIC+ACID'>DWZ</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">blaP, penP, penP blaP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1402 "Denitrobacillus licheniformis" (Weigmann 1898) Verhoeven 1952])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9l OCA], [https://pdbe.org/4n9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n9l RCSB], [https://www.ebi.ac.uk/pdbsum/4n9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n9l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9l OCA], [https://pdbe.org/4n9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n9l RCSB], [https://www.ebi.ac.uk/pdbsum/4n9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n9l ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BLAC_BACLI BLAC_BACLI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Beta-lactamase~~]]	+	[[Category: Bacillus licheniformis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Pan, X]]	+	[[Category: Pan X]]
-	[[Category: Wong, W]]	+	[[Category: Wong W]]
-	[[Category: Zhao, Y]]	+	[[Category: Zhao Y]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-antibiotic complex~~]]	+

OCA at 07:27, 9 February 2022

2022-02-09T07:27:40Z

←Older revision		Revision as of 07:27, 9 February 2022
Line 3:		Line 3:
	<StructureSection load='4n9l' size='340' side='right'caption='[[4n9l]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4n9l' size='340' side='right'caption='[[4n9l]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n9l]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"~~clostridium_licheniforme~~"~~_weigmann_1898~~ "~~clostridium licheniforme~~" weigmann 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N9L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4N9L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4n9l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"denitrobacillus_licheniformis"_(weigmann_1898)_verhoeven_1952 "denitrobacillus licheniformis" (weigmann 1898) verhoeven 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N9L FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DWZ:(2S,3R,4S)-4-{[(3S,5S)-5-(DIMETHYLCARBAMOYL)PYRROLIDIN-3-YL]SULFANYL}-2-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-3-METHYL-3,4-DIHYDRO-2H-PYRROLE-5-CARBOXYLIC+ACID'>DWZ</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DWZ:(2S,3R,4S)-4-{[(3S,5S)-5-(DIMETHYLCARBAMOYL)PYRROLIDIN-3-YL]SULFANYL}-2-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-3-METHYL-3,4-DIHYDRO-2H-PYRROLE-5-CARBOXYLIC+ACID'>DWZ</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n92\|4n92]], [[4n9k\|4n9k]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4n92\|4n92]], [[4n9k\|4n9k]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">blaP, penP, penP blaP ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1402 "~~Clostridium licheniforme~~" Weigmann 1898])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">blaP, penP, penP blaP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1402 "Denitrobacillus licheniformis" (Weigmann 1898) Verhoeven 1952])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4n9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9l OCA], [~~http~~://pdbe.org/4n9l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4n9l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4n9l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4n9l ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9l OCA], [https://pdbe.org/4n9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n9l RCSB], [https://www.ebi.ac.uk/pdbsum/4n9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n9l ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Beta-lactamase\|Beta-lactamase]]	+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Clostridium licheniforme weigmann 1898]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 08:07, 10 April 2019

2019-04-10T08:07:25Z

←Older revision		Revision as of 08:07, 10 April 2019
Line 1:		Line 1:

	==crystal structure of beta-lactamse PenP_E166S in complex with meropenem==		==crystal structure of beta-lactamse PenP_E166S in complex with meropenem==
-	<StructureSection load='4n9l' size='340' side='right' caption='[[4n9l]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='4n9l' size='340' side='right'caption='[[4n9l]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4n9l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N9L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N9L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4n9l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N9L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N9L FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DWZ:(2S,3R,4S)-4-{[(3S,5S)-5-(DIMETHYLCARBAMOYL)PYRROLIDIN-3-YL]SULFANYL}-2-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-3-METHYL-3,4-DIHYDRO-2H-PYRROLE-5-CARBOXYLIC+ACID'>DWZ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DWZ:(2S,3R,4S)-4-{[(3S,5S)-5-(DIMETHYLCARBAMOYL)PYRROLIDIN-3-YL]SULFANYL}-2-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-3-METHYL-3,4-DIHYDRO-2H-PYRROLE-5-CARBOXYLIC+ACID'>DWZ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n92\|4n92]], [[4n9k\|4n9k]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n92\|4n92]], [[4n9k\|4n9k]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">blaP, penP, penP blaP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1402 "Clostridium licheniforme" Weigmann 1898])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9l OCA], [http://pdbe.org/4n9l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n9l RCSB], [http://www.ebi.ac.uk/pdbsum/4n9l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n9l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9l OCA], [http://pdbe.org/4n9l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n9l RCSB], [http://www.ebi.ac.uk/pdbsum/4n9l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n9l ProSAT]</span></td></tr>
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Clostridium licheniforme weigmann 1898]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
		+	[[Category: Large Structures]]
	[[Category: Pan, X]]		[[Category: Pan, X]]
	[[Category: Wong, W]]		[[Category: Wong, W]]

OCA at 16:46, 11 August 2016

2016-08-11T16:46:52Z

←Older revision		Revision as of 16:46, 11 August 2016
Line 1:		Line 1:
		+
	==crystal structure of beta-lactamse PenP_E166S in complex with meropenem==		==crystal structure of beta-lactamse PenP_E166S in complex with meropenem==
	<StructureSection load='4n9l' size='340' side='right' caption='[[4n9l]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4n9l' size='340' side='right' caption='[[4n9l]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n92\|4n92]], [[4n9k\|4n9k]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n92\|4n92]], [[4n9k\|4n9k]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n9l RCSB], [http://www.ebi.ac.uk/pdbsum/4n9l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9l OCA], [http://pdbe.org/4n9l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4n9l RCSB], [http://www.ebi.ac.uk/pdbsum/4n9l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4n9l ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4n9l" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactamase\|Beta-lactamase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:00, 20 January 2015

2015-01-20T11:00:17Z

←Older revision		Revision as of 11:00, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4n9l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N9L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N9L FirstGlance]. <br>		<table><tr><td colspan='2'>[[4n9l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N9L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N9L FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DWZ:(2S,3R,4S)-4-{[(3S,5S)-5-(DIMETHYLCARBAMOYL)PYRROLIDIN-3-YL]SULFANYL}-2-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-3-METHYL-3,4-DIHYDRO-2H-PYRROLE-5-CARBOXYLIC+ACID'>DWZ</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DWZ:(2S,3R,4S)-4-{[(3S,5S)-5-(DIMETHYLCARBAMOYL)PYRROLIDIN-3-YL]SULFANYL}-2-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-3-METHYL-3,4-DIHYDRO-2H-PYRROLE-5-CARBOXYLIC+ACID'>DWZ</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n92\|4n92]], [[4n9k\|4n9k]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n92\|4n92]], [[4n9k\|4n9k]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n9l RCSB], [http://www.ebi.ac.uk/pdbsum/4n9l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n9l RCSB], [http://www.ebi.ac.uk/pdbsum/4n9l PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
-	[[Category: Pan, X.]]	+	[[Category: Pan, X]]
-	[[Category: Wong, W.]]	+	[[Category: Wong, W]]
-	[[Category: Zhao, Y.]]	+	[[Category: Zhao, Y]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Hydrolase-antibiotic complex]]		[[Category: Hydrolase-antibiotic complex]]

OCA at 09:48, 10 September 2014

2014-09-10T09:48:43Z

←Older revision		Revision as of 09:48, 10 September 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==crystal structure of beta-lactamse PenP_E166S in complex with meropenem==
		+	<StructureSection load='4n9l' size='340' side='right' caption='[[4n9l]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4n9l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N9L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N9L FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DWZ:(2S,3R,4S)-4-{[(3S,5S)-5-(DIMETHYLCARBAMOYL)PYRROLIDIN-3-YL]SULFANYL}-2-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-3-METHYL-3,4-DIHYDRO-2H-PYRROLE-5-CARBOXYLIC+ACID'>DWZ</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4n92\|4n92]], [[4n9k\|4n9k]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n9l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n9l RCSB], [http://www.ebi.ac.uk/pdbsum/4n9l PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Most class A beta-lactamases cannot hydrolyze carbapenem antibiotics effectively. The molecular mechanism of this catalytic inefficiency has been attributed to the unique stereochemistry of carbapenems, including their 6-alpha-hydroxyethyl side chain and the transition between two tautomeric states when bound at the active site. Previous studies have shown that the 6-alpha-hydroxyethyl side chain of carbapenems can interfere with catalysis by forming hydrogen bonds with the deacylation water molecule to reduce its nucleophilicity. Here our studies of a class A noncarbapenemase PenP demonstrate that substituting the general base residue Glu166 with Ser or other residues leads to a significant enhancement of the acylation kinetics by approximately 100-500 times toward carbapenems like meropenem. The structures of PenP and Glu166Ser both in apo form and in complex with meropenem reveal that Glu166 is critical for the formation of a hydrogen bonding network within the active site that locks Asn170 in an orientation to impose steric clash with the 6-alpha-hydroxyethyl side chain of meropenem. The Glu166Ser substitution weakens this network and enables Asn170 to adopt an alternative conformation to avoid steric clash and accommodate faster acylation kinetics. Furthermore, the weakened hydrogen bonding network caused by the Glu166Ser substitution allows the 6-alpha-hydroxyethyl moiety to adopt a catalytically favorable orientation as seen in class A carbapenemases. In summary, our data identify a previously unreported role of the universally conserved general base residue Glu166 in impeding the proper binding of carbapenems by restricting their 6-alpha-hydroxyethyl group.

-	~~The entry 4n9l is ON HOLD until Paper Publication~~	+	Perturbing the General Base Residue Glu166 in the Active Site of Class A beta-Lactamase Leads to Enhanced Carbapenem Binding and Acylation.,Pan X, Wong WT, He Y, Jiang Y, Zhao Y Biochemistry. 2014 Aug 26;53(33):5414-23. doi: 10.1021/bi401609h. Epub 2014 Aug, 15. PMID:25020031<ref>PMID:25020031</ref>

-	~~Authors~~: Pan, X., Wong, W., Zhao, Y.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~crystal structure of beta~~-~~lactamse PenP_E166S in~~ complex ~~with meropenem~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Beta-lactamase]]
		+	[[Category: Pan, X.]]
		+	[[Category: Wong, W.]]
		+	[[Category: Zhao, Y.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase-antibiotic complex]]

OCA at 03:44, 4 September 2014

2014-09-04T03:44:30Z

←Older revision		Revision as of 03:44, 4 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4n9l is ON HOLD	+	The entry 4n9l is ON HOLD until Paper Publication

	Authors: Pan, X., Wong, W., Zhao, Y.		Authors: Pan, X., Wong, W., Zhao, Y.

	Description: crystal structure of beta-lactamse PenP_E166S in complex with meropenem		Description: crystal structure of beta-lactamse PenP_E166S in complex with meropenem

OCA at 13:02, 11 December 2013

2013-12-11T13:02:35Z

←Older revision		Revision as of 13:02, 11 December 2013
Line 3:		Line 3:
	The entry 4n9l is ON HOLD		The entry 4n9l is ON HOLD

-	Authors: ~~Xuehua~~ Pan, ~~Waiting~~ Wong, ~~Yanxiang~~ Zhao	+	Authors: Pan, X., Wong, W., Zhao, Y.

	Description: crystal structure of beta-lactamse PenP_E166S in complex with meropenem		Description: crystal structure of beta-lactamse PenP_E166S in complex with meropenem

OCA: Protected "4n9l" [edit=sysop:move=sysop]

2013-11-06T07:04:57Z

Protected "4n9l" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:04, 6 November 2013

OCA: New page: '''Unreleased structure''' The entry 4n9l is ON HOLD Authors: Xuehua Pan, Waiting Wong, Yanxiang Zhao Description: crystal structure of beta-lactamse PenP_E166S in complex with meropen...

2013-11-06T07:04:56Z

New page: '''Unreleased structure''' The entry 4n9l is ON HOLD Authors: Xuehua Pan, Waiting Wong, Yanxiang Zhao Description: crystal structure of beta-lactamse PenP_E166S in complex with meropen...

New page

'''Unreleased structure'''

The entry 4n9l is ON HOLD

Authors: Xuehua Pan, Waiting Wong, Yanxiang Zhao

Description: crystal structure of beta-lactamse PenP_E166S in complex with meropenem