4nab - Revision history

OCA at 08:16, 11 January 2023

2023-01-11T08:16:54Z

←Older revision		Revision as of 08:16, 11 January 2023
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	==Structure of the (SR)Ca2+-ATPase mutant E309Q in the Ca2-E1-MgAMPPCP form==		==Structure of the (SR)Ca2+-ATPase mutant E309Q in the Ca2-E1-MgAMPPCP form==
-	<StructureSection load='4nab' size='340' side='right' caption='[[4nab]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<StructureSection load='4nab' size='340' side='right'caption='[[4nab]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4nab]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~European_rabbit European rabbit~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NAB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4NAB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4nab]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NAB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NAB FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PCW:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PCW</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PCW:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PCW</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n8g\|3n8g]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nab OCA], [https://pdbe.org/4nab PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nab RCSB], [https://www.ebi.ac.uk/pdbsum/4nab PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nab ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ATP2A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4nab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nab OCA], [~~http~~://pdbe.org/4nab PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4nab RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4nab PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4nab ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT]] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).	+	[https://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 20:

	==See Also==		==See Also==
-	*[[ATPase\|ATPase]]	+	*[[ATPase 3D structures\|ATPase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Calcium-transporting ATPase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~European rabbit~~]]	+	[[Category: Oryctolagus cuniculus]]
-	[[Category: Andersen~~, J P~~]]	+	[[Category: Andersen JP]]
-	[[Category: Arnou, B]]	+	[[Category: Arnou B]]
-	[[Category: Bublitz, M]]	+	[[Category: Bublitz M]]
-	[[Category: Clausen~~, J D~~]]	+	[[Category: Clausen JD]]
-	[[Category: Jaxel, C]]	+	[[Category: Jaxel C]]
-	~~[[Category: Maire, M le~~]]	+	[[Category: Moller JV]]
-	[[Category: Moller~~, J V~~]]	+	[[Category: Montigny C]]
-	[[Category: Montigny, C]]	+	[[Category: Nissen P]]
-	[[Category: Nissen, P]]	+	[[Category: Le Maire M]]
-	[[Category: ~~Calcium-transporting atpase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Mutant e309q]]~~	+
-	~~[[Category: P-type atpase]]~~	+
-	~~[[Category: Sarcoplasmic reticulum~~]]	+

OCA at 12:03, 7 October 2017

2017-10-07T12:03:11Z

←Older revision		Revision as of 12:03, 7 October 2017
Line 36:		Line 36:
	[[Category: Jaxel, C]]		[[Category: Jaxel, C]]
	[[Category: Maire, M le]]		[[Category: Maire, M le]]
		+	[[Category: Moller, J V]]
	[[Category: Montigny, C]]		[[Category: Montigny, C]]
-	[[Category: Mueller, J V]]
	[[Category: Nissen, P]]		[[Category: Nissen, P]]
	[[Category: Calcium-transporting atpase]]		[[Category: Calcium-transporting atpase]]

OCA at 11:39, 4 August 2016

2016-08-04T11:39:23Z

←Older revision		Revision as of 11:39, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of the (SR)Ca2+-ATPase mutant E309Q in the Ca2-E1-MgAMPPCP form==		==Structure of the (SR)Ca2+-ATPase mutant E309Q in the Ca2-E1-MgAMPPCP form==
	<StructureSection load='4nab' size='340' side='right' caption='[[4nab]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='4nab' size='340' side='right' caption='[[4nab]], [[Resolution\|resolution]] 3.50Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ATP2A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ATP2A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nab OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nab RCSB], [http://www.ebi.ac.uk/pdbsum/4nab PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nab OCA], [http://pdbe.org/4nab PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nab RCSB], [http://www.ebi.ac.uk/pdbsum/4nab PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nab ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4nab" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[ATPase\|ATPase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:01, 24 December 2014

2014-12-24T14:01:13Z

←Older revision		Revision as of 14:01, 24 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nab OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nab RCSB], [http://www.ebi.ac.uk/pdbsum/4nab PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nab OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nab RCSB], [http://www.ebi.ac.uk/pdbsum/4nab PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT]] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:35, 21 December 2014

2014-12-21T18:35:25Z

←Older revision		Revision as of 18:35, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4nab\| PDB=4nab \| SCENE= }}~~	+	==Structure of the (SR)Ca2+-ATPase mutant E309Q in the Ca2-E1-MgAMPPCP form==
-	===Structure of the (SR)Ca2+-ATPase mutant E309Q in the Ca2-E1-MgAMPPCP form===	+	<StructureSection load='4nab' size='340' side='right' caption='[[4nab]], [[Resolution\|resolution]] 3.50Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24270570}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4nab]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/European_rabbit European rabbit]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NAB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NAB FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PCW:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PCW</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3n8g\|3n8g]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ATP2A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9986 European rabbit])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nab OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nab RCSB], [http://www.ebi.ac.uk/pdbsum/4nab PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) couples ATP hydrolysis to transport of Ca(2+). This directed energy transfer requires cross-talk between the two Ca(2+) sites and the phosphorylation site over 50 A distance. We have addressed the mechano-structural basis for this intramolecular signal by analysing the structure and the functional properties of SERCA mutant E309Q. Glu(309) contributes to Ca(2+) coordination at site II, and a consensus has been that E309Q only binds Ca(2+) at site I. The crystal structure of E309Q in the presence of Ca(2+) and an ATP analogue, however, reveals two occupied Ca(2+) sites of a non-catalytic Ca2E1 state. Ca(2+) is bound with micromolar affinity by both Ca(2+) sites in E309Q, but without cooperativity. The Ca(2+)-bound mutant does phosphorylate from ATP, but at a very low maximal rate. Phosphorylation depends on the correct positioning of the A-domain, requiring a shift of transmembrane segment M1 into an 'up and kinked position'. This transition is impaired in the E309Q mutant, most likely due to a lack of charge neutralization and altered hydrogen binding capacities at Ca(2+) site II.

-	~~==Function==~~	+	SERCA mutant E309Q binds two Ca(2+) ions but adopts a catalytically incompetent conformation.,Clausen JD, Bublitz M, Arnou B, Montigny C, Jaxel C, Moller JV, Nissen P, Andersen JP, le Maire M EMBO J. 2013 Dec 11;32(24):3231-43. doi: 10.1038/emboj.2013.250. Epub 2013 Nov, 22. PMID:24270570<ref>PMID:24270570</ref>
-	~~[[http://www~~.~~uniprot~~.~~org/uniprot/AT2A1_RABIT AT2A1_RABIT]] This magnesium~~-~~dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen~~. ~~Contributes to calcium sequestration involved in muscular excitation~~/~~contraction (By similarity)~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4nab]] is~~ a ~~1 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4NAB OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:024270570</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Calcium-transporting ATPase]]		[[Category: Calcium-transporting ATPase]]
-	[[Category: Andersen, J P.]]	+	[[Category: European rabbit]]
-	[[Category: Arnou, B.]]	+	[[Category: Andersen, J P]]
-	[[Category: Bublitz, M.]]	+	[[Category: Arnou, B]]
-	[[Category: Clausen, J D.]]	+	[[Category: Bublitz, M]]
-	[[Category: Jaxel, C.]]	+	[[Category: Clausen, J D]]
-	[[Category: Maire, M le.]]	+	[[Category: Jaxel, C]]
-	[[Category: Montigny, C.]]	+	[[Category: Maire, M le]]
-	[[Category: Mueller, J V.]]	+	[[Category: Montigny, C]]
-	[[Category: Nissen, P.]]	+	[[Category: Mueller, J V]]
		+	[[Category: Nissen, P]]
	[[Category: Calcium-transporting atpase]]		[[Category: Calcium-transporting atpase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 10:27, 18 December 2013

2013-12-18T10:27:09Z

←Older revision		Revision as of 10:27, 18 December 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4nab\| PDB=4nab \| SCENE= }}
		+	===Structure of the (SR)Ca2+-ATPase mutant E309Q in the Ca2-E1-MgAMPPCP form===
		+	{{ABSTRACT_PUBMED_24270570}}

-	~~The entry 4nab is ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/AT2A1_RABIT AT2A1_RABIT]] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction (By similarity).

-	~~Authors~~: ~~Bublitz, M., Clausen, J.D., Arnou, B., Montigny, C., Jaxel, C., Nissen, P., Mueller, J.V~~.~~, Andersen, J~~.P.~~, le Maire, M~~.	+	==About this Structure==
		+	[[4nab]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NAB OCA].

-	~~Description~~: ~~Structure of the (SR)Ca2+~~-ATPase ~~mutant E309Q in the Ca2~~-E1-~~MgAMPPCP form~~	+	==Reference==
		+	<ref group="xtra">PMID:024270570</ref><references group="xtra"/><references/>
		+	[[Category: Calcium-transporting ATPase]]
		+	[[Category: Andersen, J P.]]
		+	[[Category: Arnou, B.]]
		+	[[Category: Bublitz, M.]]
		+	[[Category: Clausen, J D.]]
		+	[[Category: Jaxel, C.]]
		+	[[Category: Maire, M le.]]
		+	[[Category: Montigny, C.]]
		+	[[Category: Mueller, J V.]]
		+	[[Category: Nissen, P.]]
		+	[[Category: Calcium-transporting atpase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Mutant e309q]]
		+	[[Category: P-type atpase]]
		+	[[Category: Sarcoplasmic reticulum]]

OCA at 12:56, 20 November 2013

2013-11-20T12:56:28Z

←Older revision		Revision as of 12:56, 20 November 2013
Line 3:		Line 3:
	The entry 4nab is ON HOLD		The entry 4nab is ON HOLD

-	Authors: Bublitz, M., Clausen, J.D., Arnou, B., Montigny, C., Jaxel, C., Nissen, P., ~~M ller~~, J.V., Andersen, J.P., le Maire, M.	+	Authors: Bublitz, M., Clausen, J.D., Arnou, B., Montigny, C., Jaxel, C., Nissen, P., Mueller, J.V., Andersen, J.P., le Maire, M.

	Description: Structure of the (SR)Ca2+-ATPase mutant E309Q in the Ca2-E1-MgAMPPCP form		Description: Structure of the (SR)Ca2+-ATPase mutant E309Q in the Ca2-E1-MgAMPPCP form

OCA: Protected "4nab" [edit=sysop:move=sysop]

2013-11-06T07:05:53Z

Protected "4nab" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:05, 6 November 2013

OCA: New page: '''Unreleased structure''' The entry 4nab is ON HOLD Authors: Bublitz, M., Clausen, J.D., Arnou, B., Montigny, C., Jaxel, C., Nissen, P., M ller, J.V., Andersen, J.P., le Maire, M. Des...

2013-11-06T07:05:52Z

New page: '''Unreleased structure''' The entry 4nab is ON HOLD Authors: Bublitz, M., Clausen, J.D., Arnou, B., Montigny, C., Jaxel, C., Nissen, P., M ller, J.V., Andersen, J.P., le Maire, M. Des...

New page

'''Unreleased structure'''

The entry 4nab is ON HOLD

Authors: Bublitz, M., Clausen, J.D., Arnou, B., Montigny, C., Jaxel, C., Nissen, P., M ller, J.V., Andersen, J.P., le Maire, M.

Description: Structure of the (SR)Ca2+-ATPase mutant E309Q in the Ca2-E1-MgAMPPCP form