4ngj - Revision history

OCA at 16:56, 20 September 2023

2023-09-20T16:56:52Z

←Older revision		Revision as of 16:56, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ngj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NGJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ngj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NGJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=RB:RUBIDIUM+ION'>RB</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=RB:RUBIDIUM+ION'>RB</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ngj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngj OCA], [https://pdbe.org/4ngj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ngj RCSB], [https://www.ebi.ac.uk/pdbsum/4ngj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ngj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ngj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngj OCA], [https://pdbe.org/4ngj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ngj RCSB], [https://www.ebi.ac.uk/pdbsum/4ngj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ngj ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:27, 11 January 2023

2023-01-11T08:27:50Z

←Older revision		Revision as of 08:27, 11 January 2023
Line 3:		Line 3:
	<StructureSection load='4ngj' size='340' side='right'caption='[[4ngj]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='4ngj' size='340' side='right'caption='[[4ngj]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ngj]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4NGJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ngj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NGJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=RB:RUBIDIUM+ION'>RB</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=RB:RUBIDIUM+ION'>RB</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4neb\|4neb]], [[4nfv\|4nfv]], [[4ng1\|4ng1]], [[4ng8\|4ng8]], [[4ngi\|4ngi]], [[4ngk\|4ngk]], [[4ngl\|4ngl]], [[4ngo\|4ngo]], [[4ngv\|4ngv]], [[4ngw\|4ngw]], [[4ngy\|4ngy]], [[4ngz\|4ngz]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ngj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngj OCA], [https://pdbe.org/4ngj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ngj RCSB], [https://www.ebi.ac.uk/pdbsum/4ngj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ngj ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ngj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngj OCA], [~~http~~://pdbe.org/4ngj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ngj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ngj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ngj ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>	+	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 29:		Line 27:
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lysozyme]]~~	+	[[Category: Benas P]]
-	[[Category: Benas, P]]	+	[[Category: Legrand L]]
-	[[Category: Legrand, L]]	+	[[Category: Ries-Kautt M]]
-	[[Category: Ries-Kautt, M]]	+
-	~~[[Category: Esi-mass spectrometry]]~~	+
-	~~[[Category: Hofmeister series]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protein cation interaction~~]]	+

OCA at 12:10, 17 July 2019

2019-07-17T12:10:47Z

←Older revision		Revision as of 12:10, 17 July 2019
Line 1:		Line 1:

	==Dialyzed HEW lysozyme batch crystallized in 1.0 M RbCl and collected at 100 K==		==Dialyzed HEW lysozyme batch crystallized in 1.0 M RbCl and collected at 100 K==
-	<StructureSection load='4ngj' size='340' side='right' caption='[[4ngj]], [[Resolution\|resolution]] 1.10Å' scene=''>	+	<StructureSection load='4ngj' size='340' side='right'caption='[[4ngj]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ngj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NGJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ngj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NGJ FirstGlance]. <br>
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4ngj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4ngj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
		+	[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Benas, P]]		[[Category: Benas, P]]

OCA at 22:38, 5 August 2016

2016-08-05T22:38:02Z

←Older revision		Revision as of 22:38, 5 August 2016
Line 1:		Line 1:
		+
	==Dialyzed HEW lysozyme batch crystallized in 1.0 M RbCl and collected at 100 K==		==Dialyzed HEW lysozyme batch crystallized in 1.0 M RbCl and collected at 100 K==
	<StructureSection load='4ngj' size='340' side='right' caption='[[4ngj]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='4ngj' size='340' side='right' caption='[[4ngj]], [[Resolution\|resolution]] 1.10Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4neb\|4neb]], [[4nfv\|4nfv]], [[4ng1\|4ng1]], [[4ng8\|4ng8]], [[4ngi\|4ngi]], [[4ngk\|4ngk]], [[4ngl\|4ngl]], [[4ngo\|4ngo]], [[4ngv\|4ngv]], [[4ngw\|4ngw]], [[4ngy\|4ngy]], [[4ngz\|4ngz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4neb\|4neb]], [[4nfv\|4nfv]], [[4ng1\|4ng1]], [[4ng8\|4ng8]], [[4ngi\|4ngi]], [[4ngk\|4ngk]], [[4ngl\|4ngl]], [[4ngo\|4ngo]], [[4ngv\|4ngv]], [[4ngw\|4ngw]], [[4ngy\|4ngy]], [[4ngz\|4ngz]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ngj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ngj RCSB], [http://www.ebi.ac.uk/pdbsum/4ngj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ngj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngj OCA], [http://pdbe.org/4ngj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ngj RCSB], [http://www.ebi.ac.uk/pdbsum/4ngj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ngj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ngj" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:27, 25 December 2014

2014-12-25T06:27:09Z

←Older revision		Revision as of 06:27, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ngj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NGJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ngj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NGJ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=RB:RUBIDIUM+ION'>RB</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=RB:RUBIDIUM+ION'>RB</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4neb\|4neb]], [[4nfv\|4nfv]], [[4ng1\|4ng1]], [[4ng8\|4ng8]], [[4ngi\|4ngi]], [[4ngk\|4ngk]], [[4ngl\|4ngl]], [[4ngo\|4ngo]], [[4ngv\|4ngv]], [[4ngw\|4ngw]], [[4ngy\|4ngy]], [[4ngz\|4ngz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4neb\|4neb]], [[4nfv\|4nfv]], [[4ng1\|4ng1]], [[4ng8\|4ng8]], [[4ngi\|4ngi]], [[4ngk\|4ngk]], [[4ngl\|4ngl]], [[4ngo\|4ngo]], [[4ngv\|4ngv]], [[4ngw\|4ngw]], [[4ngy\|4ngy]], [[4ngz\|4ngz]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ngj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ngj RCSB], [http://www.ebi.ac.uk/pdbsum/4ngj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ngj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ngj RCSB], [http://www.ebi.ac.uk/pdbsum/4ngj PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 24:
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
-	[[Category: Benas, P.]]	+	[[Category: Benas, P]]
-	[[Category: Legrand, L.]]	+	[[Category: Legrand, L]]
-	[[Category: Ries-Kautt, M.]]	+	[[Category: Ries-Kautt, M]]
	[[Category: Esi-mass spectrometry]]		[[Category: Esi-mass spectrometry]]
	[[Category: Hofmeister series]]		[[Category: Hofmeister series]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Protein cation interaction]]		[[Category: Protein cation interaction]]

OCA at 06:24, 13 August 2014

2014-08-13T06:24:02Z

←Older revision		Revision as of 06:24, 13 August 2014
Line 10:		Line 10:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	The ~~influence~~ of ~~salt nature and concentration on tetragonal lysozyme chloride crystal solubility is presented for a set of mono-, di- and trivalent cations (Cs~~(+), Rb(+), Mn(2+), Co(2+) and Yb(3+)). The ~~results show that~~ cations ~~have as strong an effect on protein solubility as anions and that they present their own particular effects as co-ions~~. ~~Indeed, after decreasing~~ at ~~low ionic strength~~, ~~lysozyme solubility~~ increases ~~with high concentration~~ of ~~polyvalent~~ cations, ~~probably due to co-ion binding and therefore the concomitant increase~~ of the ~~net charge~~ of the protein-~~salt complex~~. ~~These new results are discussed in order~~ to ~~progress in~~ the ~~understanding~~ of the ~~crystallisation process at~~ the ~~atomic level~~.	+	The adsorption of Rb(+), Cs(+), Mn(2+), Co(2+) and Yb(3+) onto the positively charged hen egg-white lysozyme (HEWL) has been investigated by solving 13 X-ray structures of HEWL crystallized with their chlorides and by applying electrospray ionization mass spectrometry (ESI-MS) first to dissolved protein crystals and then to the protein in buffered salt solutions. The number of bound cations follows the order Cs(+) < Mn(2+) approximately Co(2+) < Yb(3+) at 293 K. HEWL binds less Rb(+) (qtot = 0.7) than Cs(+) (qtot = 3.9) at 100 K. Crystal flash-cooling drastically increases the binding of Cs(+), but poorly affects that of Yb(3+), suggesting different interactions. The addition of glycerol increases the number of bound Yb(3+) cations, but only slightly increases that of Rb(+). HEWL titrations with the same chlorides, followed by ESI-MS analysis, show that only about 10% of HEWL binds Cs(+) and about 40% binds 1-2 Yb(3+) cations, while the highest binding reaches 60-70% for protein binding 1-3 Mn(2+) or Co(2+) cations. The binding sites identified by X-ray crystallography show that the monovalent Rb(+) and Cs(+) preferentially bind to carbonyl groups, whereas the multivalent Mn(2+), Co(2+) and Yb(3+) interact with carboxylic groups. This work elucidates the basis of the effect of the Hofmeister cation series on protein solubility.

-	~~Strong~~ and ~~specific effects of cations on lysozyme chloride solubility~~.,Benas P, Legrand L, Ries-Kautt M Acta Crystallogr D Biol Crystallogr. ~~2002 Oct~~;58(Pt ~~10 Pt 1~~):~~1582~~-7. ~~Epub 2002~~, ~~Sep 26~~. PMID:~~12351866~~<ref>PMID:~~12351866~~</ref>	+	Weak protein-cationic co-ion interactions addressed by X-ray crystallography and mass spectrometry.,Benas P, Auzeil N, Legrand L, Brachet F, Regazzetti A, Ries-Kautt M Acta Crystallogr D Biol Crystallogr. 2014 Aug 1;70(Pt 8):2217-31. doi:, 10.1107/S1399004714011304. Epub 2014 Jul 25. PMID:25084340<ref>PMID:25084340</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 09:38, 28 May 2014

2014-05-28T09:38:17Z

←Older revision		Revision as of 09:38, 28 May 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Dialyzed HEW lysozyme batch crystallized in 1.0 M RbCl and collected at 100 K==
		+	<StructureSection load='4ngj' size='340' side='right' caption='[[4ngj]], [[Resolution\|resolution]] 1.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ngj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NGJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NGJ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=RB:RUBIDIUM+ION'>RB</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4neb\|4neb]], [[4nfv\|4nfv]], [[4ng1\|4ng1]], [[4ng8\|4ng8]], [[4ngi\|4ngi]], [[4ngk\|4ngk]], [[4ngl\|4ngl]], [[4ngo\|4ngo]], [[4ngv\|4ngv]], [[4ngw\|4ngw]], [[4ngy\|4ngy]], [[4ngz\|4ngz]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ngj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ngj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ngj RCSB], [http://www.ebi.ac.uk/pdbsum/4ngj PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The influence of salt nature and concentration on tetragonal lysozyme chloride crystal solubility is presented for a set of mono-, di- and trivalent cations (Cs(+), Rb(+), Mn(2+), Co(2+) and Yb(3+)). The results show that cations have as strong an effect on protein solubility as anions and that they present their own particular effects as co-ions. Indeed, after decreasing at low ionic strength, lysozyme solubility increases with high concentration of polyvalent cations, probably due to co-ion binding and therefore the concomitant increase of the net charge of the protein-salt complex. These new results are discussed in order to progress in the understanding of the crystallisation process at the atomic level.

-	~~The entry 4ngj is ON HOLD until Paper Publication~~	+	Strong and specific effects of cations on lysozyme chloride solubility.,Benas P, Legrand L, Ries-Kautt M Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 1):1582-7. Epub 2002, Sep 26. PMID:12351866<ref>PMID:12351866</ref>

-	~~Authors~~: Benas, P., Legrand, L., Ries-Kautt, M.	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Dialyzed HEW lysozyme batch crystallized in 1.0 M RbCl and collected at 100 K~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gallus gallus]]
		+	[[Category: Lysozyme]]
		+	[[Category: Benas, P.]]
		+	[[Category: Legrand, L.]]
		+	[[Category: Ries-Kautt, M.]]
		+	[[Category: Esi-mass spectrometry]]
		+	[[Category: Hofmeister series]]
		+	[[Category: Hydrolase]]
		+	[[Category: Protein cation interaction]]

OCA at 06:48, 4 December 2013

2013-12-04T06:48:55Z

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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ngj is ON HOLD	+	The entry 4ngj is ON HOLD until Paper Publication

	Authors: Benas, P., Legrand, L., Ries-Kautt, M.		Authors: Benas, P., Legrand, L., Ries-Kautt, M.

	Description: Dialyzed HEW lysozyme batch crystallized in 1.0 M RbCl and collected at 100 K		Description: Dialyzed HEW lysozyme batch crystallized in 1.0 M RbCl and collected at 100 K

OCA: Protected "4ngj" [edit=sysop:move=sysop]

2013-11-13T07:48:04Z

Protected "4ngj" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:48, 13 November 2013

OCA: New page: '''Unreleased structure''' The entry 4ngj is ON HOLD Authors: Benas, P., Legrand, L., Ries-Kautt, M. Description: Dialyzed HEW lysozyme batch crystallized in 1.0 M RbCl and collected a...

2013-11-13T07:48:03Z

New page: '''Unreleased structure''' The entry 4ngj is ON HOLD Authors: Benas, P., Legrand, L., Ries-Kautt, M. Description: Dialyzed HEW lysozyme batch crystallized in 1.0 M RbCl and collected a...

New page

'''Unreleased structure'''

The entry 4ngj is ON HOLD

Authors: Benas, P., Legrand, L., Ries-Kautt, M.

Description: Dialyzed HEW lysozyme batch crystallized in 1.0 M RbCl and collected at 100 K