4npj - Revision history

OCA at 12:34, 1 March 2024

2024-03-01T12:34:39Z

←Older revision		Revision as of 12:34, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4npj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NPJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NPJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4npj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NPJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NPJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.101Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4npj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4npj OCA], [https://pdbe.org/4npj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4npj RCSB], [https://www.ebi.ac.uk/pdbsum/4npj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4npj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4npj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4npj OCA], [https://pdbe.org/4npj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4npj RCSB], [https://www.ebi.ac.uk/pdbsum/4npj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4npj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ESYT2_HUMAN ESYT2_HUMAN] May play a role as calcium-regulated intrinsic membrane protein.<ref>PMID:17360437</ref>		[https://www.uniprot.org/uniprot/ESYT2_HUMAN ESYT2_HUMAN] May play a role as calcium-regulated intrinsic membrane protein.<ref>PMID:17360437</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Contacts between the endoplasmic reticulum and the plasma membrane involve extended synaptotagmins (E-Syts) in mammals or tricalbins in yeast, proteins with multiple C2 domains. One of the tandem C2 domains of E-Syt2 is predicted to bind Ca2+, but no Ca2+-dependent function has been attributed to this protein. We have determined the crystal structures of the tandem C2 domains of E-Syt2 in the absence and presence of Ca2+ and analyzed their Ca2+-binding properties by nuclear magnetic resonance spectroscopy. Our data reveal an unexpected V-shaped structure with a rigid orientation between the two C2 domains that is not substantially altered by Ca2+. The E-Syt2 C2A domain binds up to four Ca2+ ions, whereas the C2B domain does not bind Ca2+. These results suggest that E-Syt2 performs an as yet unidentified Ca2+-dependent function through its C2A domain and uncover fundamental differences between the properties of the tandem C2 domains of E-Syts and synaptotagmins.
-
-	Structure and Ca-Binding Properties of the Tandem C Domains of E-Syt2.,Xu J, Bacaj T, Zhou A, Tomchick DR, Sudhof TC, Rizo J Structure. 2013 Dec 24. pii: S0969-2126(13)00461-9. doi:, 10.1016/j.str.2013.11.011. PMID:24373768<ref>PMID:24373768</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4npj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 08:40, 11 January 2023

2023-01-11T08:40:10Z

←Older revision		Revision as of 08:40, 11 January 2023
Line 1:		Line 1:

	==Extended-Synaptotagmin 2, C2A- and C2B-domains==		==Extended-Synaptotagmin 2, C2A- and C2B-domains==
-	<StructureSection load='4npj' size='340' side='right' caption='[[4npj]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='4npj' size='340' side='right'caption='[[4npj]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4npj]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NPJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4NPJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4npj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NPJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NPJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4npk\|4npk]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4npj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4npj OCA], [https://pdbe.org/4npj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4npj RCSB], [https://www.ebi.ac.uk/pdbsum/4npj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4npj ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ESYT2, extended-synaptotagmin 2, FAM62B, KIAA1228 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4npj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4npj OCA], [~~http~~://pdbe.org/4npj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4npj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4npj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4npj ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ESYT2_HUMAN ESYT2_HUMAN]] May play a role as calcium-regulated intrinsic membrane protein.<ref>PMID:17360437</ref>	+	[https://www.uniprot.org/uniprot/ESYT2_HUMAN ESYT2_HUMAN] May play a role as calcium-regulated intrinsic membrane protein.<ref>PMID:17360437</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4npj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4npj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Synaptotagmin 3D structures\|Synaptotagmin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Rizo, J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Tomchick, D R]]~~	+	[[Category: Rizo J]]
-	~~[[Category: Xu,~~ J]]	+	[[Category: Tomchick DR]]
-	[[Category: ~~C2 domain~~]]	+	[[Category: Xu J]]
-	[[Category: ~~Calcium/phospholipid binding protein]]~~	+
-	~~[[Category: Er to plasma membrane]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Membrane traffic]]~~	+
-	~~[[Category: Plasma membrane]]~~	+
-	~~[[Category: Protein targeting~~]]	+

OCA at 11:51, 5 August 2016

2016-08-05T11:51:38Z

←Older revision		Revision as of 11:51, 5 August 2016
Line 1:		Line 1:
		+
	==Extended-Synaptotagmin 2, C2A- and C2B-domains==		==Extended-Synaptotagmin 2, C2A- and C2B-domains==
	<StructureSection load='4npj' size='340' side='right' caption='[[4npj]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='4npj' size='340' side='right' caption='[[4npj]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4npk\|4npk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4npk\|4npk]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ESYT2, extended-synaptotagmin 2, FAM62B, KIAA1228 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ESYT2, extended-synaptotagmin 2, FAM62B, KIAA1228 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4npj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4npj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4npj RCSB], [http://www.ebi.ac.uk/pdbsum/4npj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4npj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4npj OCA], [http://pdbe.org/4npj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4npj RCSB], [http://www.ebi.ac.uk/pdbsum/4npj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4npj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4npj" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:11, 25 December 2014

2014-12-25T02:11:57Z

←Older revision		Revision as of 02:11, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4npj\| PDB=4npj \| SCENE= }}~~	+	==Extended-Synaptotagmin 2, C2A- and C2B-domains==
-	===Extended-Synaptotagmin 2, C2A- and C2B-domains===	+	<StructureSection load='4npj' size='340' side='right' caption='[[4npj]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24373768}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4npj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NPJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NPJ FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4npk\|4npk]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ESYT2, extended-synaptotagmin 2, FAM62B, KIAA1228 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4npj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4npj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4npj RCSB], [http://www.ebi.ac.uk/pdbsum/4npj PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/ESYT2_HUMAN ESYT2_HUMAN]] May play a role as calcium-regulated intrinsic membrane protein.<ref>PMID:17360437</ref>		[[http://www.uniprot.org/uniprot/ESYT2_HUMAN ESYT2_HUMAN]] May play a role as calcium-regulated intrinsic membrane protein.<ref>PMID:17360437</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Contacts between the endoplasmic reticulum and the plasma membrane involve extended synaptotagmins (E-Syts) in mammals or tricalbins in yeast, proteins with multiple C2 domains. One of the tandem C2 domains of E-Syt2 is predicted to bind Ca2+, but no Ca2+-dependent function has been attributed to this protein. We have determined the crystal structures of the tandem C2 domains of E-Syt2 in the absence and presence of Ca2+ and analyzed their Ca2+-binding properties by nuclear magnetic resonance spectroscopy. Our data reveal an unexpected V-shaped structure with a rigid orientation between the two C2 domains that is not substantially altered by Ca2+. The E-Syt2 C2A domain binds up to four Ca2+ ions, whereas the C2B domain does not bind Ca2+. These results suggest that E-Syt2 performs an as yet unidentified Ca2+-dependent function through its C2A domain and uncover fundamental differences between the properties of the tandem C2 domains of E-Syts and synaptotagmins.

-	~~==About this~~ Structure==	+	Structure and Ca-Binding Properties of the Tandem C Domains of E-Syt2.,Xu J, Bacaj T, Zhou A, Tomchick DR, Sudhof TC, Rizo J Structure. 2013 Dec 24. pii: S0969-2126(13)00461-9. doi:, 10.1016/j.str.2013.11.011. PMID:24373768<ref>PMID:24373768</ref>
-	~~[[4npj]] is a 2 chain structure~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.~~il/oca-bin/ocashort?id=4NPJ OCA]~~.	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024373768</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
-	[[Category: Rizo, J.]]	+	== References ==
-	[[Category: Tomchick, D R.]]	+	<references/>
-	[[Category: Xu, J.]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Rizo, J]]
		+	[[Category: Tomchick, D R]]
		+	[[Category: Xu, J]]
	[[Category: C2 domain]]		[[Category: C2 domain]]
	[[Category: Calcium/phospholipid binding protein]]		[[Category: Calcium/phospholipid binding protein]]

OCA at 07:36, 29 January 2014

2014-01-29T07:36:36Z

←Older revision		Revision as of 07:36, 29 January 2014
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4npj\| PDB=4npj \| SCENE= }}
		+	===Extended-Synaptotagmin 2, C2A- and C2B-domains===
		+	{{ABSTRACT_PUBMED_24373768}}

-	~~The entry 4npj is ON HOLD~~ ~~until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/ESYT2_HUMAN ESYT2_HUMAN]] May play a role as calcium-regulated intrinsic membrane protein.<ref>PMID:17360437</ref>

-	~~Authors~~: ~~Tomchick, D~~.R.~~, Rizo, J~~.~~, Xu, J~~.	+	==About this Structure==
		+	[[4npj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NPJ OCA].

-	~~Description~~: ~~Extended-Synaptotagmin 2~~, ~~C2A-and C2B-domains~~	+	==Reference==
		+	<ref group="xtra">PMID:024373768</ref><references group="xtra"/><references/>
		+	[[Category: Rizo, J.]]
		+	[[Category: Tomchick, D R.]]
		+	[[Category: Xu, J.]]
		+	[[Category: C2 domain]]
		+	[[Category: Calcium/phospholipid binding protein]]
		+	[[Category: Er to plasma membrane]]
		+	[[Category: Membrane protein]]
		+	[[Category: Membrane traffic]]
		+	[[Category: Plasma membrane]]
		+	[[Category: Protein targeting]]

OCA at 06:51, 4 December 2013

2013-12-04T06:51:26Z

←Older revision		Revision as of 06:51, 4 December 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4npj is ON HOLD	+	The entry 4npj is ON HOLD until Paper Publication

	Authors: Tomchick, D.R., Rizo, J., Xu, J.		Authors: Tomchick, D.R., Rizo, J., Xu, J.

	Description: Extended-Synaptotagmin 2, C2A-and C2B-domains		Description: Extended-Synaptotagmin 2, C2A-and C2B-domains

OCA: Protected "4npj" [edit=sysop:move=sysop]

2013-11-27T09:54:40Z

Protected "4npj" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:54, 27 November 2013

OCA: New page: '''Unreleased structure''' The entry 4npj is ON HOLD Authors: Tomchick, D.R., Rizo, J., Xu, J. Description: Extended-Synaptotagmin 2, C2A-and C2B-domains

2013-11-27T09:54:39Z

New page: '''Unreleased structure''' The entry 4npj is ON HOLD Authors: Tomchick, D.R., Rizo, J., Xu, J. Description: Extended-Synaptotagmin 2, C2A-and C2B-domains

New page

'''Unreleased structure'''

The entry 4npj is ON HOLD

Authors: Tomchick, D.R., Rizo, J., Xu, J.

Description: Extended-Synaptotagmin 2, C2A-and C2B-domains