4nyr - Revision history

OCA at 08:46, 18 January 2023

2023-01-18T08:46:34Z

←Older revision		Revision as of 08:46, 18 January 2023
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	<StructureSection load='4nyr' size='340' side='right'caption='[[4nyr]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='4nyr' size='340' side='right'caption='[[4nyr]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4nyr]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Diploptera_punctata Diploptera punctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NYR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4NYR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4nyr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Diploptera_punctata Diploptera punctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NYR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4nyq\|4nyq]], [[4nys\|4nys]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nyr OCA], [https://pdbe.org/4nyr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nyr RCSB], [https://www.ebi.ac.uk/pdbsum/4nyr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nyr ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4nyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nyr OCA], [~~http~~://pdbe.org/4nyr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4nyr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4nyr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4nyr ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6SVB6_DIPPU Q6SVB6_DIPPU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 24:
	[[Category: Diploptera punctata]]		[[Category: Diploptera punctata]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chavas~~, L M.G~~]]	+	[[Category: Chavas LMG]]
-	[[Category: Coussens~~, N P~~]]	+	[[Category: Coussens NP]]
-	[[Category: Gallat, F X]]	+	[[Category: Gallat F-X]]
-	[[Category: Ramaswamy, S]]	+	[[Category: Ramaswamy S]]
-	[[Category: Stay, B]]	+	[[Category: Stay B]]
-	[[Category: Tobe~~, S S~~]]	+	[[Category: Tobe SS]]
-	[[Category: Yagi, K]]	+	[[Category: Yagi K]]
-	~~[[Category: Cockroach]]~~	+
-	~~[[Category: Fatty acid binding protein]]~~	+
-	~~[[Category: In-vivo crystallization]]~~	+
-	~~[[Category: Lipid binding protein]]~~	+
-	~~[[Category: Lipocalin]]~~	+
-	~~[[Category: Lipocalin fold]]~~	+
-	~~[[Category: Sad phasing~~]]	+

OCA at 08:38, 1 January 2020

2020-01-01T08:38:54Z

←Older revision		Revision as of 08:38, 1 January 2020
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	==In-vivo crystallisation (midguts of a viviparous cockroach) and structure at 2.5 A resolution of a glycosylated, lipid-binding, lipocalin-like protein==		==In-vivo crystallisation (midguts of a viviparous cockroach) and structure at 2.5 A resolution of a glycosylated, lipid-binding, lipocalin-like protein==
-	<StructureSection load='4nyr' size='340' side='right' caption='[[4nyr]], [[Resolution\|resolution]] 2.49Å' scene=''>	+	<StructureSection load='4nyr' size='340' side='right'caption='[[4nyr]], [[Resolution\|resolution]] 2.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4nyr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diploptera_punctata Diploptera punctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NYR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NYR FirstGlance]. <br>		<table><tr><td colspan='2'>[[4nyr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diploptera_punctata Diploptera punctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NYR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NYR FirstGlance]. <br>
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nyr OCA], [http://pdbe.org/4nyr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nyr RCSB], [http://www.ebi.ac.uk/pdbsum/4nyr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nyr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nyr OCA], [http://pdbe.org/4nyr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nyr RCSB], [http://www.ebi.ac.uk/pdbsum/4nyr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nyr ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Macromolecular crystals for X-ray diffraction studies are typically grown in vitro from pure and homogeneous samples; however, there are examples of protein crystals that have been identified in vivo. Recent developments in micro-crystallography techniques and the advent of X-ray free-electron lasers have allowed the determination of several protein structures from crystals grown in cellulo. Here, an atomic resolution (1.2 A) crystal structure is reported of heterogeneous milk proteins grown inside a living organism in their functional niche. These in vivo-grown crystals were isolated from the midgut of an embryo within the only known viviparous cockroach, Diploptera punctata. The milk proteins crystallized in space group P1, and a structure was determined by anomalous dispersion from the native S atoms. The data revealed glycosylated proteins that adopt a lipocalin fold, bind lipids and organize to form a tightly packed crystalline lattice. A single crystal is estimated to contain more than three times the energy of an equivalent mass of dairy milk. This unique storage form of nourishment for developing embryos allows access to a constant supply of complete nutrients. Notably, the crystalline cockroach-milk proteins are highly heterogeneous with respect to amino-acid sequence, glycosylation and bound fatty-acid composition. These data present a unique example of protein heterogeneity within a single in vivo-grown crystal of a natural protein in its native environment at atomic resolution.
		+
		+	Structure of a heterogeneous, glycosylated, lipid-bound, in vivo-grown protein crystal at atomic resolution from the viviparous cockroach Diploptera punctata.,Banerjee S, Coussens NP, Gallat FX, Sathyanarayanan N, Srikanth J, Yagi KJ, Gray JS, Tobe SS, Stay B, Chavas LM, Ramaswamy S IUCrJ. 2016 Jun 27;3(Pt 4):282-93. doi: 10.1107/S2052252516008903. eCollection, 2016 Jul 1. PMID:27437115<ref>PMID:27437115</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4nyr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Diploptera punctata]]		[[Category: Diploptera punctata]]
		+	[[Category: Large Structures]]
	[[Category: Chavas, L M.G]]		[[Category: Chavas, L M.G]]
	[[Category: Coussens, N P]]		[[Category: Coussens, N P]]

OCA at 06:24, 26 July 2016

2016-07-26T06:24:52Z

←Older revision		Revision as of 06:24, 26 July 2016
Line 1:		Line 1:
		+
	==In-vivo crystallisation (midguts of a viviparous cockroach) and structure at 2.5 A resolution of a glycosylated, lipid-binding, lipocalin-like protein==		==In-vivo crystallisation (midguts of a viviparous cockroach) and structure at 2.5 A resolution of a glycosylated, lipid-binding, lipocalin-like protein==
	<StructureSection load='4nyr' size='340' side='right' caption='[[4nyr]], [[Resolution\|resolution]] 2.49Å' scene=''>		<StructureSection load='4nyr' size='340' side='right' caption='[[4nyr]], [[Resolution\|resolution]] 2.49Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4nyq\|4nyq]], [[4nys\|4nys]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4nyq\|4nyq]], [[4nys\|4nys]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nyr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nyr RCSB], [http://www.ebi.ac.uk/pdbsum/4nyr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nyr OCA], [http://pdbe.org/4nyr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nyr RCSB], [http://www.ebi.ac.uk/pdbsum/4nyr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nyr ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 17:47, 21 December 2014

2014-12-21T17:47:43Z

←Older revision		Revision as of 17:47, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4nyr\| PDB=4nyr \| SCENE= }}~~	+	==In-vivo crystallisation (midguts of a viviparous cockroach) and structure at 2.5 A resolution of a glycosylated, lipid-binding, lipocalin-like protein==
-	===In-vivo crystallisation (midguts of a viviparous cockroach) and structure at 2.5 A resolution of a glycosylated, lipid-binding, lipocalin-like protein===	+	<StructureSection load='4nyr' size='340' side='right' caption='[[4nyr]], [[Resolution\|resolution]] 2.49Å' scene=''>
-		+	== Structural highlights ==
-	==~~About this Structure~~==	+	<table><tr><td colspan='2'>[[4nyr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diploptera_punctata Diploptera punctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NYR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NYR FirstGlance]. <br>
-	[[4nyr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diploptera_punctata Diploptera punctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NYR OCA].	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4nyq\|4nyq]], [[4nys\|4nys]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nyr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nyr RCSB], [http://www.ebi.ac.uk/pdbsum/4nyr PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Diploptera punctata]]		[[Category: Diploptera punctata]]
-	[[Category: Chavas, L M.G.]]	+	[[Category: Chavas, L M.G]]
-	[[Category: Coussens, N P.]]	+	[[Category: Coussens, N P]]
-	[[Category: Gallat, F X.]]	+	[[Category: Gallat, F X]]
-	[[Category: Ramaswamy, S.]]	+	[[Category: Ramaswamy, S]]
-	[[Category: Stay, B.]]	+	[[Category: Stay, B]]
-	[[Category: Tobe, S S.]]	+	[[Category: Tobe, S S]]
-	[[Category: Yagi, K.]]	+	[[Category: Yagi, K]]
	[[Category: Cockroach]]		[[Category: Cockroach]]
	[[Category: Fatty acid binding protein]]		[[Category: Fatty acid binding protein]]

OCA at 12:17, 1 January 2014

2014-01-01T12:17:16Z

←Older revision		Revision as of 12:17, 1 January 2014
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-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4nyr\| PDB=4nyr \| SCENE= }}
		+	===In-vivo crystallisation (midguts of a viviparous cockroach) and structure at 2.5 A resolution of a glycosylated, lipid-binding, lipocalin-like protein===

-	~~The entry~~ 4nyr is ~~ON HOLD~~	+	==About this Structure==
-		+	[[4nyr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diploptera_punctata Diploptera punctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NYR OCA].
-	~~Authors~~: Coussens, N.P., Gallat, F.-X., Ramaswamy, S., ~~Yagi, K~~., Tobe, S.S., ~~Stay, B., Chavas, L.M.G~~.	+	[[Category: Diploptera punctata]]
-		+	[[Category: Chavas, L M.G.]]
-	~~Description~~: In-vivo ~~crystallisation (midguts of a viviparous cockroach) and structure at 2.5 A resolution of a glycosylated, lipid-~~binding~~, lipocalin-like~~ protein	+	[[Category: Coussens, N P.]]
		+	[[Category: Gallat, F X.]]
		+	[[Category: Ramaswamy, S.]]
		+	[[Category: Stay, B.]]
		+	[[Category: Tobe, S S.]]
		+	[[Category: Yagi, K.]]
		+	[[Category: Cockroach]]
		+	[[Category: Fatty acid binding protein]]
		+	[[Category: In-vivo crystallization]]
		+	[[Category: Lipid binding protein]]
		+	[[Category: Lipocalin]]
		+	[[Category: Lipocalin fold]]
		+	[[Category: Sad phasing]]

OCA: Protected "4nyr" [edit=sysop:move=sysop]

2013-12-18T09:27:56Z

Protected "4nyr" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:27, 18 December 2013

OCA: New page: '''Unreleased structure''' The entry 4nyr is ON HOLD Authors: Coussens, N.P., Gallat, F.-X., Ramaswamy, S., Yagi, K., Tobe, S.S., Stay, B., Chavas, L.M.G. Description: In-vivo crystall...

2013-12-18T09:27:54Z

New page: '''Unreleased structure''' The entry 4nyr is ON HOLD Authors: Coussens, N.P., Gallat, F.-X., Ramaswamy, S., Yagi, K., Tobe, S.S., Stay, B., Chavas, L.M.G. Description: In-vivo crystall...

New page

'''Unreleased structure'''

The entry 4nyr is ON HOLD

Authors: Coussens, N.P., Gallat, F.-X., Ramaswamy, S., Yagi, K., Tobe, S.S., Stay, B., Chavas, L.M.G.

Description: In-vivo crystallisation (midguts of a viviparous cockroach) and structure at 2.5 A resolution of a glycosylated, lipid-binding, lipocalin-like protein