4p22 - Revision history

OCA at 00:39, 28 December 2023

2023-12-28T00:39:21Z

←Older revision		Revision as of 00:39, 28 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4p22]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P22 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4p22]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P22 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p22 OCA], [https://pdbe.org/4p22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p22 RCSB], [https://www.ebi.ac.uk/pdbsum/4p22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p22 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.75Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p22 OCA], [https://pdbe.org/4p22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p22 RCSB], [https://www.ebi.ac.uk/pdbsum/4p22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p22 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==

OCA at 12:37, 1 February 2023

2023-02-01T12:37:44Z

←Older revision		Revision as of 12:37, 1 February 2023
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	<StructureSection load='4p22' size='340' side='right'caption='[[4p22]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='4p22' size='340' side='right'caption='[[4p22]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4p22]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P22 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4P22 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4p22]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P22 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBA1, A1S9T, UBE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p22 OCA], [https://pdbe.org/4p22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p22 RCSB], [https://www.ebi.ac.uk/pdbsum/4p22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p22 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4p22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p22 OCA], [~~http~~://pdbe.org/4p22 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4p22 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4p22 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4p22 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/UBA1_HUMAN UBA1_HUMAN]] X-linked distal arthrogryposis multiplex congenita. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/UBA1_HUMAN UBA1_HUMAN] X-linked distal arthrogryposis multiplex congenita. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/UBA1_HUMAN UBA1_HUMAN]] Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.<ref>PMID:22456334</ref>	+	[https://www.uniprot.org/uniprot/UBA1_HUMAN UBA1_HUMAN] Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.<ref>PMID:22456334</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[Ubiquitin activating enzyme\|Ubiquitin activating enzyme]]	+	*[[3D structures of Ubiquitin activating enzyme\|3D structures of Ubiquitin activating enzyme]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Xie, S T]]	+	[[Category: Xie S-T]]
-	~~[[Category: E1]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Ubiquitin~~]]	+

OCA at 07:44, 8 January 2020

2020-01-08T07:44:25Z

←Older revision		Revision as of 07:44, 8 January 2020
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	<StructureSection load='4p22' size='340' side='right'caption='[[4p22]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='4p22' size='340' side='right'caption='[[4p22]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4p22]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P22 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4p22]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P22 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p22 OCA], [http://pdbe.org/4p22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p22 RCSB], [http://www.ebi.ac.uk/pdbsum/4p22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p22 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBA1, A1S9T, UBE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p22 OCA], [http://pdbe.org/4p22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p22 RCSB], [http://www.ebi.ac.uk/pdbsum/4p22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p22 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 26:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Xie, S T]]		[[Category: Xie, S T]]

OCA at 07:22, 10 July 2019

2019-07-10T07:22:28Z

←Older revision		Revision as of 07:22, 10 July 2019
Line 1:		Line 1:

	==Crystal Structure of N-terminal Fragments of E1==		==Crystal Structure of N-terminal Fragments of E1==
-	<StructureSection load='4p22' size='340' side='right' caption='[[4p22]], [[Resolution\|resolution]] 2.75Å' scene=''>	+	<StructureSection load='4p22' size='340' side='right'caption='[[4p22]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4p22]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P22 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4p22]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P22 FirstGlance]. <br>
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4p22" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4p22" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ubiquitin activating enzyme\|Ubiquitin activating enzyme]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Xie, S T]]		[[Category: Xie, S T]]
	[[Category: E1]]		[[Category: E1]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Ubiquitin]]		[[Category: Ubiquitin]]

OCA at 17:11, 4 January 2017

2017-01-04T17:11:06Z

←Older revision		Revision as of 17:11, 4 January 2017
Line 1:		Line 1:
		+
	==Crystal Structure of N-terminal Fragments of E1==		==Crystal Structure of N-terminal Fragments of E1==
	<StructureSection load='4p22' size='340' side='right' caption='[[4p22]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='4p22' size='340' side='right' caption='[[4p22]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4p22]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P22 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4p22]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P22 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p22 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p22 RCSB], [http://www.ebi.ac.uk/pdbsum/4p22 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p22 OCA], [http://pdbe.org/4p22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p22 RCSB], [http://www.ebi.ac.uk/pdbsum/4p22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p22 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4p22" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:55, 25 February 2015

2015-02-25T13:55:12Z

←Older revision		Revision as of 13:55, 25 February 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal Structure of N-terminal Fragments of E1==
		+	<StructureSection load='4p22' size='340' side='right' caption='[[4p22]], [[Resolution\|resolution]] 2.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4p22]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P22 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p22 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p22 RCSB], [http://www.ebi.ac.uk/pdbsum/4p22 PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/UBA1_HUMAN UBA1_HUMAN]] X-linked distal arthrogryposis multiplex congenita. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/UBA1_HUMAN UBA1_HUMAN]] Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.<ref>PMID:22456334</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ubiquitin-activating enzyme (E1) is a key regulator in protein ubiquitination, which lies on the upstream of the ubiquitin-related pathways and determines the activation of the downstream enzyme cascade. Thus far, no structural information about the human ubiquitin-activating enzyme has been reported. We expressed and purified the N-terminal domains of human E1 and determined their crystal structures, which contain inactive adenylation domain (IAD) and the first catalytic cysteine half-domain (FCCH). This study presents the crystal structure of human E1 fragment for the first time. The main structure of both IAD and FCCH superimposed well with their corresponding domains in yeast Uba1, but their relative positions vary significantly. This work provides new structural insights in understanding the mechanisms of ubiquitin activation in humans.

-	~~The entry 4p22 is ON HOLD until Paper Publication~~	+	Expression, purification, and crystal structure of N-terminal domains of human ubiquitin-activating enzyme (E1).,Xie ST Biosci Biotechnol Biochem. 2014;78(9):1542-9. doi: 10.1080/09168451.2014.923301. , Epub 2014 Jun 9. PMID:25209502<ref>PMID:25209502</ref>

-	~~Authors: Xie~~, S.-T.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal Structure of N-terminal Fragments of~~ E1	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Xie, S.-T~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Xie, S T]]
		+	[[Category: E1]]
		+	[[Category: Ligase]]
		+	[[Category: Ubiquitin]]

OCA at 14:43, 24 December 2014

2014-12-24T14:43:41Z

←Older revision		Revision as of 14:43, 24 December 2014
Line 6:		Line 6:

	Description: Crystal Structure of N-terminal Fragments of E1		Description: Crystal Structure of N-terminal Fragments of E1
		+	[[Category: Unreleased Structures]]
		+	[[Category: Xie, S.-T]]

OCA at 00:52, 7 August 2014

2014-08-07T00:52:35Z

←Older revision		Revision as of 00:52, 7 August 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4p22 is ON HOLD	+	The entry 4p22 is ON HOLD until Paper Publication

-	Authors: ~~Shu-Tao~~ Xie	+	Authors: Xie, S.-T.

	Description: Crystal Structure of N-terminal Fragments of E1		Description: Crystal Structure of N-terminal Fragments of E1

OCA: Protected "4p22" [edit=sysop:move=sysop]

2014-03-12T12:29:12Z

Protected "4p22" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:29, 12 March 2014

OCA: New page: '''Unreleased structure''' The entry 4p22 is ON HOLD Authors: Shu-Tao Xie Description: Crystal Structure of N-terminal Fragments of E1

2014-03-12T12:29:11Z

New page: '''Unreleased structure''' The entry 4p22 is ON HOLD Authors: Shu-Tao Xie Description: Crystal Structure of N-terminal Fragments of E1

New page

'''Unreleased structure'''

The entry 4p22 is ON HOLD

Authors: Shu-Tao Xie

Description: Crystal Structure of N-terminal Fragments of E1