4pa5 - Revision history

OCA at 07:19, 1 May 2024

2024-05-01T07:19:18Z

←Older revision		Revision as of 07:19, 1 May 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/TGL_BACSU TGL_BACSU] Probably plays a role in the assembly of the spore coat proteins by catalyzing epsilon-(gamma-glutamyl)lysine cross-links. In wild-type spores at 37 degrees Celsius, tgl mediates the cross-linking of GerQ in higher molecular mass forms, probably in cooperation with YabG.		[https://www.uniprot.org/uniprot/TGL_BACSU TGL_BACSU] Probably plays a role in the assembly of the spore coat proteins by catalyzing epsilon-(gamma-glutamyl)lysine cross-links. In wild-type spores at 37 degrees Celsius, tgl mediates the cross-linking of GerQ in higher molecular mass forms, probably in cooperation with YabG.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Transglutaminases are best known for their ability to catalyze protein cross-linking reactions that impart chemical and physical resilience to cellular structures. Here, we report the crystal structure and characterization of Tgl, a transglutaminase from the bacterium Bacillus subtilis. Tgl is produced during sporulation and cross-links the surface of the highly resilient spore. Tgl-like proteins are found only in spore-forming bacteria of the Bacillus and Clostridia classes, indicating an ancient origin. Tgl is a single-domain protein, produced in active form, and the smallest transglutaminase characterized to date. We show that Tgl is structurally similar to bacterial cell wall endopeptidases and has an NlpC/P60 catalytic core, thought to represent the ancestral unit of the cysteine protease fold. We show that Tgl functions through a unique partially redundant catalytic dyad formed by Cys116 and Glu187 or Glu115. Strikingly, the catalytic Cys is insulated within a hydrophobic tunnel that traverses the molecule from side to side. The lack of similarity of Tgl to other transglutaminases together with its small size suggests that an NlpC/P60 catalytic core and insulation of the active site during catalysis may be essential requirements for protein cross-linking.
-
-	Structural and Functional Characterization of an Ancient Bacterial Transglutaminase Sheds Light on the Minimal Requirements for Protein Cross-Linking.,Fernandes CG, Placido D, Lousa D, Brito JA, Isidro A, Soares CM, Pohl J, Carrondo MA, Archer M, Henriques AO Biochemistry. 2015 Sep 22;54(37):5723-34. doi: 10.1021/acs.biochem.5b00661. Epub , 2015 Sep 8. PMID:26322858<ref>PMID:26322858</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4pa5" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 00:41, 28 December 2023

2023-12-28T00:41:55Z

←Older revision		Revision as of 00:41, 28 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pa5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PA5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PA5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pa5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PA5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PA5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DHL:2-AMINO-ETHANETHIOL'>DHL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.86Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DHL:2-AMINO-ETHANETHIOL'>DHL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pa5 OCA], [https://pdbe.org/4pa5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pa5 RCSB], [https://www.ebi.ac.uk/pdbsum/4pa5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pa5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pa5 OCA], [https://pdbe.org/4pa5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pa5 RCSB], [https://www.ebi.ac.uk/pdbsum/4pa5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pa5 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:49, 1 February 2023

2023-02-01T12:49:22Z

←Older revision		Revision as of 12:49, 1 February 2023
Line 1:		Line 1:

	==Tgl - a bacterial spore coat transglutaminase - cystamine complex==		==Tgl - a bacterial spore coat transglutaminase - cystamine complex==
-	<StructureSection load='4pa5' size='340' side='right' caption='[[4pa5]], [[Resolution\|resolution]] 1.86Å' scene=''>	+	<StructureSection load='4pa5' size='340' side='right'caption='[[4pa5]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pa5]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacsu Bacsu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PA5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PA5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pa5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PA5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PA5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DHL:2-AMINO-ETHANETHIOL'>DHL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DHL:2-AMINO-ETHANETHIOL'>DHL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4p8i\|4p8i]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pa5 OCA], [https://pdbe.org/4pa5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pa5 RCSB], [https://www.ebi.ac.uk/pdbsum/4pa5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pa5 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tgl, yugV, yuxF, BSU31270 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-glutamine_gamma-glutamyltransferase Protein-glutamine gamma-glutamyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.13 2.3.2.13] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4pa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pa5 OCA], [~~http~~://pdbe.org/4pa5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4pa5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4pa5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4pa5 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TGL_BACSU TGL_BACSU]] Probably plays a role in the assembly of the spore coat proteins by catalyzing epsilon-(gamma-glutamyl)lysine cross-links. In wild-type spores at 37 degrees Celsius, tgl mediates the cross-linking of GerQ in higher molecular mass forms, probably in cooperation with YabG.	+	[https://www.uniprot.org/uniprot/TGL_BACSU TGL_BACSU] Probably plays a role in the assembly of the spore coat proteins by catalyzing epsilon-(gamma-glutamyl)lysine cross-links. In wild-type spores at 37 degrees Celsius, tgl mediates the cross-linking of GerQ in higher molecular mass forms, probably in cooperation with YabG.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacsu~~]]	+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
-	[[Category: ~~Protein-glutamine gamma-glutamyltransferase~~]]	+	[[Category: Large Structures]]
-	[[Category: Archer, M]]	+	[[Category: Archer M]]
-	[[Category: Brito~~, J A~~]]	+	[[Category: Brito JA]]
-	[[Category: Carrondo~~, M A~~]]	+	[[Category: Carrondo MA]]
-	[[Category: Fernandes~~, C G~~]]	+	[[Category: Fernandes CG]]
-	[[Category: Henriques~~, A O~~]]	+	[[Category: Henriques AO]]
-	[[Category: Isidro, A]]	+	[[Category: Isidro A]]
-	[[Category: Lousa, D]]	+	[[Category: Lousa D]]
-	[[Category: Placido, D]]	+	[[Category: Placido D]]
-	[[Category: Pohl, J]]	+	[[Category: Pohl J]]
-	[[Category: Soares~~, C M]]~~	+	[[Category: Soares CM]]
-	~~[[Category: Baterial spore coat]]~~	+
-	~~[[Category: Nlpc/p60 endopeptidase]]~~	+
-	~~[[Category: Papain]]~~	+
-	~~[[Category: Protein cross-linking]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:37, 22 November 2017

2017-11-22T08:37:00Z

←Older revision		Revision as of 08:37, 22 November 2017
Line 1:		Line 1:
		+
	==Tgl - a bacterial spore coat transglutaminase - cystamine complex==		==Tgl - a bacterial spore coat transglutaminase - cystamine complex==
	<StructureSection load='4pa5' size='340' side='right' caption='[[4pa5]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='4pa5' size='340' side='right' caption='[[4pa5]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pa5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PA5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PA5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pa5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PA5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PA5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DHL:2-AMINO-ETHANETHIOL'>DHL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DHL:2-AMINO-ETHANETHIOL'>DHL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4p8i\|4p8i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4p8i\|4p8i]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">tgl, yugV, yuxF, BSU31270 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-glutamine_gamma-glutamyltransferase Protein-glutamine gamma-glutamyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.13 2.3.2.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-glutamine_gamma-glutamyltransferase Protein-glutamine gamma-glutamyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.13 2.3.2.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pa5 OCA], [http://pdbe.org/4pa5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pa5 RCSB], [http://www.ebi.ac.uk/pdbsum/4pa5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pa5 OCA], [http://pdbe.org/4pa5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pa5 RCSB], [http://www.ebi.ac.uk/pdbsum/4pa5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pa5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacsu]]
	[[Category: Protein-glutamine gamma-glutamyltransferase]]		[[Category: Protein-glutamine gamma-glutamyltransferase]]
	[[Category: Archer, M]]		[[Category: Archer, M]]

OCA at 14:30, 30 September 2015

2015-09-30T14:30:14Z

←Older revision		Revision as of 14:30, 30 September 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Tgl - a bacterial spore coat transglutaminase - cystamine complex==
		+	<StructureSection load='4pa5' size='340' side='right' caption='[[4pa5]], [[Resolution\|resolution]] 1.86Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4pa5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PA5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PA5 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DHL:2-AMINO-ETHANETHIOL'>DHL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4p8i\|4p8i]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-glutamine_gamma-glutamyltransferase Protein-glutamine gamma-glutamyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.2.13 2.3.2.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pa5 OCA], [http://pdbe.org/4pa5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pa5 RCSB], [http://www.ebi.ac.uk/pdbsum/4pa5 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TGL_BACSU TGL_BACSU]] Probably plays a role in the assembly of the spore coat proteins by catalyzing epsilon-(gamma-glutamyl)lysine cross-links. In wild-type spores at 37 degrees Celsius, tgl mediates the cross-linking of GerQ in higher molecular mass forms, probably in cooperation with YabG.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Transglutaminases are best known for their ability to catalyze protein cross-linking reactions that impart chemical and physical resilience to cellular structures. Here, we report the crystal structure and characterization of Tgl, a transglutaminase from the bacterium Bacillus subtilis. Tgl is produced during sporulation and cross-links the surface of the highly resilient spore. Tgl-like proteins are found only in spore-forming bacteria of the Bacillus and Clostridia classes, indicating an ancient origin. Tgl is a single-domain protein, produced in active form, and the smallest transglutaminase characterized to date. We show that Tgl is structurally similar to bacterial cell wall endopeptidases and has an NlpC/P60 catalytic core, thought to represent the ancestral unit of the cysteine protease fold. We show that Tgl functions through a unique partially redundant catalytic dyad formed by Cys116 and Glu187 or Glu115. Strikingly, the catalytic Cys is insulated within a hydrophobic tunnel that traverses the molecule from side to side. The lack of similarity of Tgl to other transglutaminases together with its small size suggests that an NlpC/P60 catalytic core and insulation of the active site during catalysis may be essential requirements for protein cross-linking.

-	~~The entry 4pa5 is ON HOLD until Paper Publication~~	+	Structural and Functional Characterization of an Ancient Bacterial Transglutaminase Sheds Light on the Minimal Requirements for Protein Cross-Linking.,Fernandes CG, Placido D, Lousa D, Brito JA, Isidro A, Soares CM, Pohl J, Carrondo MA, Archer M, Henriques AO Biochemistry. 2015 Sep 22;54(37):5723-34. doi: 10.1021/acs.biochem.5b00661. Epub , 2015 Sep 8. PMID:26322858<ref>PMID:26322858</ref>

-	~~Authors: Brito~~, J.A.~~, Placido, D~~., ~~Fernandes, C.G., Lousa, D., Isidro, A., Soares, C.~~M~~., Pohl~~, J., Carrondo, M.A~~., Henriques, A.O., Archer, M.~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Tgl -a bacterial spore coat transglutaminase -cystamine complex~~	+	<div class="pdbe-citations 4pa5" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
-	[[Category: Henriques, A.O]]	+	<references/>
-	[[Category: ~~Brito~~, J.A]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Protein-glutamine gamma-glutamyltransferase]]
		+	[[Category: Archer, M]]
		+	[[Category: Brito, J A]]
		+	[[Category: Carrondo, M A]]
		+	[[Category: Fernandes, C G]]
		+	[[Category: Henriques, A O]]
		+	[[Category: Isidro, A]]
		+	[[Category: Lousa, D]]
	[[Category: Placido, D]]		[[Category: Placido, D]]
-	[[Category: Lousa, D]]
-	[[Category: Fernandes, C.G]]
	[[Category: Pohl, J]]		[[Category: Pohl, J]]
-	[[Category: ~~Isidro~~, A]]	+	[[Category: Soares, C M]]
-	[[Category: ~~Carrondo, M.A~~]]	+	[[Category: Baterial spore coat]]
-	[[Category: ~~Soares, C.M~~]]	+	[[Category: Nlpc/p60 endopeptidase]]
-	[[Category: ~~Archer, M~~]]	+	[[Category: Papain]]
		+	[[Category: Protein cross-linking]]
		+	[[Category: Transferase]]

OCA at 14:53, 24 December 2014

2014-12-24T14:53:08Z

←Older revision		Revision as of 14:53, 24 December 2014
Line 6:		Line 6:

	Description: Tgl -a bacterial spore coat transglutaminase -cystamine complex		Description: Tgl -a bacterial spore coat transglutaminase -cystamine complex
		+	[[Category: Unreleased Structures]]
		+	[[Category: Henriques, A.O]]
		+	[[Category: Brito, J.A]]
		+	[[Category: Placido, D]]
		+	[[Category: Lousa, D]]
		+	[[Category: Fernandes, C.G]]
		+	[[Category: Pohl, J]]
		+	[[Category: Isidro, A]]
		+	[[Category: Carrondo, M.A]]
		+	[[Category: Soares, C.M]]
		+	[[Category: Archer, M]]

OCA at 09:13, 20 October 2014

2014-10-20T09:13:20Z

←Older revision		Revision as of 09:13, 20 October 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4pa5 is ON HOLD	+	The entry 4pa5 is ON HOLD until Paper Publication

	Authors: Brito, J.A., Placido, D., Fernandes, C.G., Lousa, D., Isidro, A., Soares, C.M., Pohl, J., Carrondo, M.A., Henriques, A.O., Archer, M.		Authors: Brito, J.A., Placido, D., Fernandes, C.G., Lousa, D., Isidro, A., Soares, C.M., Pohl, J., Carrondo, M.A., Henriques, A.O., Archer, M.

	Description: Tgl -a bacterial spore coat transglutaminase -cystamine complex		Description: Tgl -a bacterial spore coat transglutaminase -cystamine complex

OCA at 07:23, 23 April 2014

2014-04-23T07:23:33Z

←Older revision		Revision as of 07:23, 23 April 2014
Line 3:		Line 3:
	The entry 4pa5 is ON HOLD		The entry 4pa5 is ON HOLD

-	Authors: Brito, ~~Jose~~ A., Placido, ~~Diana~~, Fernandes, ~~Catarina~~ G., Lousa, ~~Diana~~, Isidro, ~~Anabela~~, Soares, ~~Claudio~~ M, Pohl, ~~Jan~~, Carrondo, ~~Maria~~ A., Henriques, ~~Adriano~~ O., Archer, ~~Margarida~~	+	Authors: Brito, J.A., Placido, D., Fernandes, C.G., Lousa, D., Isidro, A., Soares, C.M., Pohl, J., Carrondo, M.A., Henriques, A.O., Archer, M.

	Description: Tgl -a bacterial spore coat transglutaminase -cystamine complex		Description: Tgl -a bacterial spore coat transglutaminase -cystamine complex

OCA: Protected "4pa5" [edit=sysop:move=sysop]

2014-04-16T09:04:47Z

Protected "4pa5" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:04, 16 April 2014

OCA: New page: '''Unreleased structure''' The entry 4pa5 is ON HOLD Authors: Brito, Jose A., Placido, Diana, Fernandes, Catarina G., Lousa, Diana, Isidro, Anabela, Soares, Claudio M, Pohl, Jan, Carron...

2014-04-16T09:04:46Z

New page: '''Unreleased structure''' The entry 4pa5 is ON HOLD Authors: Brito, Jose A., Placido, Diana, Fernandes, Catarina G., Lousa, Diana, Isidro, Anabela, Soares, Claudio M, Pohl, Jan, Carron...

New page

'''Unreleased structure'''

The entry 4pa5 is ON HOLD

Authors: Brito, Jose A., Placido, Diana, Fernandes, Catarina G., Lousa, Diana, Isidro, Anabela, Soares, Claudio M, Pohl, Jan, Carrondo, Maria A., Henriques, Adriano O., Archer, Margarida

Description: Tgl -a bacterial spore coat transglutaminase -cystamine complex