4paa - Revision history

OCA at 07:13, 27 September 2023

2023-09-27T07:13:41Z

←Older revision		Revision as of 07:13, 27 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4paa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PAA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4paa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PAA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=THG:(6S)-5,6,7,8-TETRAHYDROFOLATE'>THG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.26Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=THG:(6S)-5,6,7,8-TETRAHYDROFOLATE'>THG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4paa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4paa OCA], [https://pdbe.org/4paa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4paa RCSB], [https://www.ebi.ac.uk/pdbsum/4paa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4paa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4paa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4paa OCA], [https://pdbe.org/4paa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4paa RCSB], [https://www.ebi.ac.uk/pdbsum/4paa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4paa ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:49, 1 February 2023

2023-02-01T12:49:34Z

←Older revision		Revision as of 12:49, 1 February 2023
Line 3:		Line 3:
	<StructureSection load='4paa' size='340' side='right'caption='[[4paa]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='4paa' size='340' side='right'caption='[[4paa]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4paa]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PAA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PAA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4paa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PAA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=THG:(6S)-5,6,7,8-TETRAHYDROFOLATE'>THG</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=THG:(6S)-5,6,7,8-TETRAHYDROFOLATE'>THG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4p9s\|4p9s]], [[4pab\|4pab]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4paa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4paa OCA], [https://pdbe.org/4paa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4paa RCSB], [https://www.ebi.ac.uk/pdbsum/4paa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4paa ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dmgdh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylglycine_dehydrogenase Dimethylglycine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.8.4 1.5.8.4] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4paa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4paa OCA], [~~http~~://pdbe.org/4paa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4paa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4paa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4paa ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/M2GD_RAT M2GD_RAT] Catalyzes the demethylation of N,N-dimethylglycine to sarcosine. Also has activity with sarcosine in vitro.<ref>PMID:2417560</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Buffalo rat]]
-	[[Category: Dimethylglycine dehydrogenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Loukachevitch~~, L V~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: Luka, Z]]	+	[[Category: Loukachevitch LV]]
-	[[Category: Newcomer~~, M E~~]]	+	[[Category: Luka Z]]
-	[[Category: Pakhomova, S]]	+	[[Category: Newcomer ME]]
-	[[Category: Wagner, C]]	+	[[Category: Pakhomova S]]
-	~~[[Category: Oxidoreductase]]~~	+	[[Category: Wagner C]]
-	~~[[Category: Rat]]~~	+
-	~~[[Category: Tetrahydrofolate~~]]	+

OCA at 10:10, 25 December 2019

2019-12-25T10:10:08Z

←Older revision		Revision as of 10:10, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of the mature form of rat DMGDH complexed with tetrahydrofolate==		==Crystal structure of the mature form of rat DMGDH complexed with tetrahydrofolate==
-	<StructureSection load='4paa' size='340' side='right' caption='[[4paa]], [[Resolution\|resolution]] 2.26Å' scene=''>	+	<StructureSection load='4paa' size='340' side='right'caption='[[4paa]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4paa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PAA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4paa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PAA FirstGlance]. <br>
Line 25:		Line 25:
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
	[[Category: Dimethylglycine dehydrogenase]]		[[Category: Dimethylglycine dehydrogenase]]
		+	[[Category: Large Structures]]
	[[Category: Loukachevitch, L V]]		[[Category: Loukachevitch, L V]]
	[[Category: Luka, Z]]		[[Category: Luka, Z]]

OCA at 11:58, 6 November 2017

2017-11-06T11:58:11Z

←Older revision		Revision as of 11:58, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='4paa' size='340' side='right' caption='[[4paa]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='4paa' size='340' side='right' caption='[[4paa]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4paa]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PAA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4paa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PAA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=THG:(6S)-5,6,7,8-TETRAHYDROFOLATE'>THG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=THG:(6S)-5,6,7,8-TETRAHYDROFOLATE'>THG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4p9s\|4p9s]], [[4pab\|4pab]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4p9s\|4p9s]], [[4pab\|4pab]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dmgdh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylglycine_dehydrogenase Dimethylglycine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.8.4 1.5.8.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylglycine_dehydrogenase Dimethylglycine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.8.4 1.5.8.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4paa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4paa OCA], [http://pdbe.org/4paa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4paa RCSB], [http://www.ebi.ac.uk/pdbsum/4paa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4paa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4paa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4paa OCA], [http://pdbe.org/4paa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4paa RCSB], [http://www.ebi.ac.uk/pdbsum/4paa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4paa ProSAT]</span></td></tr>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Buffalo rat]]
	[[Category: Dimethylglycine dehydrogenase]]		[[Category: Dimethylglycine dehydrogenase]]
	[[Category: Loukachevitch, L V]]		[[Category: Loukachevitch, L V]]
Line 28:		Line 30:
	[[Category: Pakhomova, S]]		[[Category: Pakhomova, S]]
	[[Category: Wagner, C]]		[[Category: Wagner, C]]
		+	[[Category: Oxidoreductase]]
	[[Category: Rat]]		[[Category: Rat]]
	[[Category: Tetrahydrofolate]]		[[Category: Tetrahydrofolate]]

OCA at 18:00, 11 August 2016

2016-08-11T18:00:35Z

←Older revision		Revision as of 18:00, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the mature form of rat DMGDH complexed with tetrahydrofolate==		==Crystal structure of the mature form of rat DMGDH complexed with tetrahydrofolate==
	<StructureSection load='4paa' size='340' side='right' caption='[[4paa]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='4paa' size='340' side='right' caption='[[4paa]], [[Resolution\|resolution]] 2.26Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4p9s\|4p9s]], [[4pab\|4pab]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4p9s\|4p9s]], [[4pab\|4pab]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylglycine_dehydrogenase Dimethylglycine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.8.4 1.5.8.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylglycine_dehydrogenase Dimethylglycine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.8.4 1.5.8.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4paa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4paa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4paa RCSB], [http://www.ebi.ac.uk/pdbsum/4paa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4paa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4paa OCA], [http://pdbe.org/4paa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4paa RCSB], [http://www.ebi.ac.uk/pdbsum/4paa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4paa ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4paa" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:44, 5 January 2015

2015-01-05T12:44:40Z

←Older revision		Revision as of 12:44, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4paa]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PAA FirstGlance]. <br>		<table><tr><td colspan='2'>[[4paa]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PAA FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=THG:(6S)-5,6,7,8-TETRAHYDROFOLATE'>THG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=THG:(6S)-5,6,7,8-TETRAHYDROFOLATE'>THG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4p9s\|4p9s]], [[4pab\|4pab]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4p9s\|4p9s]], [[4pab\|4pab]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylglycine_dehydrogenase Dimethylglycine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.8.4 1.5.8.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylglycine_dehydrogenase Dimethylglycine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.8.4 1.5.8.4] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4paa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4paa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4paa RCSB], [http://www.ebi.ac.uk/pdbsum/4paa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4paa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4paa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4paa RCSB], [http://www.ebi.ac.uk/pdbsum/4paa PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Dimethylglycine dehydrogenase]]		[[Category: Dimethylglycine dehydrogenase]]
-	[[Category: Loukachevitch, L V.]]	+	[[Category: Loukachevitch, L V]]
-	[[Category: Luka, Z.]]	+	[[Category: Luka, Z]]
-	[[Category: Newcomer, M E.]]	+	[[Category: Newcomer, M E]]
-	[[Category: Pakhomova, S.]]	+	[[Category: Pakhomova, S]]
-	[[Category: Wagner, C~~.]]~~	+	[[Category: Wagner, C]]
-	~~[[Category: Dimethylglycine dehydrogenase~~]]	+
	[[Category: Rat]]		[[Category: Rat]]
	[[Category: Tetrahydrofolate]]		[[Category: Tetrahydrofolate]]

OCA at 07:53, 18 June 2014

2014-06-18T07:53:09Z

←Older revision		Revision as of 07:53, 18 June 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the mature form of rat DMGDH complexed with tetrahydrofolate==
		+	<StructureSection load='4paa' size='340' side='right' caption='[[4paa]], [[Resolution\|resolution]] 2.26Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4paa]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PAA FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=THG:(6S)-5,6,7,8-TETRAHYDROFOLATE'>THG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4p9s\|4p9s]], [[4pab\|4pab]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylglycine_dehydrogenase Dimethylglycine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.8.4 1.5.8.4] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4paa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4paa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4paa RCSB], [http://www.ebi.ac.uk/pdbsum/4paa PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dimethylglycine dehydrogenase (DMGDH) is a mammalian mitochondrial enzyme which plays an important role in the utilization of methyl groups derived from choline. DMGDH is a flavin containing enzyme which catalyzes the oxidative demethylation of dimethylglycine in vitro with the formation of sarcosine (N-methylglycine), hydrogen peroxide and formaldehyde. DMGDH binds tetrahydrofolate (THF) in vivo, which serves as an acceptor of formaldehyde and in the cell the product of the reaction is 5,10-methylenetetrahydrofolate instead of formaldehyde. To gain insight into the mechanism of the reaction we solved the crystal structures of the recombinant mature and precursor forms of rat DMGDH and DMGDH-THF complexes. Both forms of DMGDH reveal similar kinetic parameters and have the same tertiary structure fold with two domains formed by N- and C-terminal halves of the protein. The active center is located in the N-terminal domain while the THF binding site is located in the C-terminal domain about 40A from the isoalloxazine ring of FAD. The folate binding site is connected with the enzyme active center via an intramolecular channel. This suggests the possible transfer of the intermediate imine of dimethylglycine from the active center to the bound THF where they could react producing a 5,10-methylenetetrahydrofolate. Based on the homology of the rat and human DMGDH the structural basis for the mechanism of inactivation of the human DMGDH by naturally occurring His109Arg mutation is proposed.

-	The ~~entry 4paa is ON HOLD until Paper Publication~~	+	Folate in demethylation: The crystal structure of the rat dimethylglycine dehydrogenase complexed with tetrahydrofolate.,Luka Z, Pakhomova S, Loukachevitch LV, Newcomer ME, Wagner C Biochem Biophys Res Commun. 2014 May 22. pii: S0006-291X(14)00936-X. doi:, 10.1016/j.bbrc.2014.05.064. PMID:24858690<ref>PMID:24858690</ref>

-	~~Authors: Luka~~, Z.~~, Pakhomova,~~ S., Loukachevitch, L.V., Newcomer, M.E., Wagner, C.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of the mature form of rat DMGDH complexed with tetrahydrofolate~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Dimethylglycine dehydrogenase]]
		+	[[Category: Loukachevitch, L V.]]
		+	[[Category: Luka, Z.]]
		+	[[Category: Newcomer, M E.]]
		+	[[Category: Pakhomova, S.]]
		+	[[Category: Wagner, C.]]
		+	[[Category: Dimethylglycine dehydrogenase]]
		+	[[Category: Rat]]
		+	[[Category: Tetrahydrofolate]]

OCA at 06:27, 30 April 2014

2014-04-30T06:27:13Z

←Older revision		Revision as of 06:27, 30 April 2014
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	Authors: Luka, Z., Pakhomova, S., Loukachevitch, L.V., Newcomer, M.E., Wagner, C.		Authors: Luka, Z., Pakhomova, S., Loukachevitch, L.V., Newcomer, M.E., Wagner, C.

-	Description: Crystal structure of the mature form of DMGDH complexed with tetrahydrofolate	+	Description: Crystal structure of the mature form of rat DMGDH complexed with tetrahydrofolate

OCA at 07:23, 23 April 2014

2014-04-23T07:23:36Z

←Older revision		Revision as of 07:23, 23 April 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4paa is ON HOLD	+	The entry 4paa is ON HOLD until Paper Publication

	Authors: Luka, Z., Pakhomova, S., Loukachevitch, L.V., Newcomer, M.E., Wagner, C.		Authors: Luka, Z., Pakhomova, S., Loukachevitch, L.V., Newcomer, M.E., Wagner, C.

	Description: Crystal structure of the mature form of DMGDH complexed with tetrahydrofolate		Description: Crystal structure of the mature form of DMGDH complexed with tetrahydrofolate

OCA: Protected "4paa" [edit=sysop:move=sysop]

2014-04-16T09:04:57Z

Protected "4paa" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:04, 16 April 2014