4poy - Revision history

OCA at 07:22, 8 February 2023

2023-02-08T07:22:15Z

←Older revision		Revision as of 07:22, 8 February 2023
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	<StructureSection load='4poy' size='340' side='right'caption='[[4poy]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='4poy' size='340' side='right'caption='[[4poy]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4poy]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Camelus_glama Camelus~~ glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4POY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4POY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4poy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4POY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4POY FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pou\|4pou]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4poy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4poy OCA], [https://pdbe.org/4poy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4poy RCSB], [https://www.ebi.ac.uk/pdbsum/4poy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4poy ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4poy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4poy OCA], [~~http~~://pdbe.org/4poy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4poy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4poy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4poy ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Camelus~~ glama]]	+	[[Category: Lama glama]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fanning~~, S W~~]]	+	[[Category: Fanning SW]]
-	[[Category: Horn~~, J R~~]]	+	[[Category: Horn JR]]
-	[[Category: Walter, R]]	+	[[Category: Walter R]]
-	~~[[Category: Affinity control]]~~	+
-	~~[[Category: Antibody engineering]]~~	+
-	~~[[Category: Antibody-metal complex]]~~	+
-	~~[[Category: Binding thermodynamic]]~~	+
-	~~[[Category: Bispecific]]~~	+
-	~~[[Category: Conformational dynamic]]~~	+
-	~~[[Category: Dual-function]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Loop dynamic]]~~	+
-	~~[[Category: Metalloregulation]]~~	+
-	~~[[Category: Nickel binding protein]]~~	+
-	~~[[Category: Protein engineering]]~~	+
-	~~[[Category: Protein flexibility]]~~	+
-	~~[[Category: Protein-metal coordination geometry]]~~	+
-	~~[[Category: Protein-switch]]~~	+
-	~~[[Category: Single domain antibody]]~~	+
-	~~[[Category: Vhh~~]]	+

OCA at 07:34, 17 April 2019

2019-04-17T07:34:15Z

←Older revision		Revision as of 07:34, 17 April 2019
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	==Engineered Dual Specific VHH antibody==		==Engineered Dual Specific VHH antibody==
-	<StructureSection load='4poy' size='340' side='right' caption='[[4poy]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='4poy' size='340' side='right'caption='[[4poy]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4poy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4POY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4POY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4poy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4POY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4POY FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pou\|4pou]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pou\|4pou]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4poy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4poy OCA], [http://pdbe.org/4poy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4poy RCSB], [http://www.ebi.ac.uk/pdbsum/4poy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4poy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4poy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4poy OCA], [http://pdbe.org/4poy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4poy RCSB], [http://www.ebi.ac.uk/pdbsum/4poy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4poy ProSAT]</span></td></tr>
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	</div>		</div>
	<div class="pdbe-citations 4poy" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4poy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camelus glama]]
		+	[[Category: Large Structures]]
	[[Category: Fanning, S W]]		[[Category: Fanning, S W]]
	[[Category: Horn, J R]]		[[Category: Horn, J R]]

OCA at 19:47, 11 August 2016

2016-08-11T19:47:50Z

←Older revision		Revision as of 19:47, 11 August 2016
Line 1:		Line 1:
		+
	==Engineered Dual Specific VHH antibody==		==Engineered Dual Specific VHH antibody==
	<StructureSection load='4poy' size='340' side='right' caption='[[4poy]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='4poy' size='340' side='right' caption='[[4poy]], [[Resolution\|resolution]] 1.50Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4poy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4POY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4POY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4poy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4POY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4POY FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pou\|4pou]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pou\|4pou]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4poy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4poy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4poy RCSB], [http://www.ebi.ac.uk/pdbsum/4poy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4poy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4poy OCA], [http://pdbe.org/4poy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4poy RCSB], [http://www.ebi.ac.uk/pdbsum/4poy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4poy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4poy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:13, 25 January 2015

2015-01-25T08:13:24Z

←Older revision		Revision as of 08:13, 25 January 2015
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Fanning, S W.]]	+	[[Category: Fanning, S W]]
-	[[Category: Horn, J R.]]	+	[[Category: Horn, J R]]
-	[[Category: Walter, R.]]	+	[[Category: Walter, R]]
	[[Category: Affinity control]]		[[Category: Affinity control]]
	[[Category: Antibody engineering]]		[[Category: Antibody engineering]]

OCA at 08:22, 22 October 2014

2014-10-22T08:22:21Z

←Older revision		Revision as of 08:22, 22 October 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4poy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4POY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4POY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4poy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4POY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4POY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pou\|4pou]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pou\|4pou]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4poy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4poy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4poy RCSB], [http://www.ebi.ac.uk/pdbsum/4poy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4poy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4poy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4poy RCSB], [http://www.ebi.ac.uk/pdbsum/4poy PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 10:29, 24 September 2014

2014-09-24T10:29:25Z

←Older revision		Revision as of 10:29, 24 September 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Engineered Dual Specific VHH antibody==
		+	<StructureSection load='4poy' size='340' side='right' caption='[[4poy]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4poy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4POY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4POY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4pou\|4pou]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4poy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4poy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4poy RCSB], [http://www.ebi.ac.uk/pdbsum/4poy PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	To explore dual-specificity in a small protein interface, we previously generated a 'metal switch' anti-RNase A VHH antibody using a combinatorial histidine library approach. While most metal-binding sites in proteins are found within rigid secondary structure, the engineered VHH antibody (VHHmetal), which contained three new histidine residues, possessed metal-binding residues within the flexible hypervariable loops. Here, crystal structure analysis of the free and bound states of VHHmetal reveals the structural determinants leading to dual-function. Most notably, CDR1 is observed in two distinct conformations when adopting the metal or RNase A bound states. Furthermore, mutagenesis studies revealed that one of the engineered residues, not located in the metal-binding pocket, contributed indirectly to metal recognition, likely through influencing CDR1 conformation. Despite these changes, VHHmetal possesses a relatively minor energetic penalty toward binding the original antigen, RNase A ( approximately 1 kcal/mol), where the engineered gain-of-function metal-binding residues are observed to possess a mix of favorable and unfavorable contributions towards RNase A recognition. Ultimately, the conformationally distinct metal-switch interface architecture reflects the robust, library-based strategy used to produce VHHmetal. These results also suggest that even small protein interfaces, such as VHH, may be structurally and energetically forgiving in adopting novel function, while maintaining original function.

-	~~The entry 4poy is ON HOLD~~	+	Structural basis of an engineered dual-specific antibody: conformational diversity leads to a hypervariable loop metal-binding site.,Fanning SW, Walter R, Horn JR Protein Eng Des Sel. 2014 Aug 20. pii: gzu033. PMID:25143596<ref>PMID:25143596</ref>

-	~~Authors~~: Fanning, S.W.~~, Walter, R.,~~ Horn, J.R.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Engineered~~ Dual ~~Specific VHH~~ antibody	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fanning, S W.]]
		+	[[Category: Horn, J R.]]
		+	[[Category: Walter, R.]]
		+	[[Category: Affinity control]]
		+	[[Category: Antibody engineering]]
		+	[[Category: Antibody-metal complex]]
		+	[[Category: Binding thermodynamic]]
		+	[[Category: Bispecific]]
		+	[[Category: Conformational dynamic]]
		+	[[Category: Dual-function]]
		+	[[Category: Immune system]]
		+	[[Category: Loop dynamic]]
		+	[[Category: Metalloregulation]]
		+	[[Category: Nickel binding protein]]
		+	[[Category: Protein engineering]]
		+	[[Category: Protein flexibility]]
		+	[[Category: Protein-metal coordination geometry]]
		+	[[Category: Protein-switch]]
		+	[[Category: Single domain antibody]]
		+	[[Category: Vhh]]

OCA: Protected "4poy" [edit=sysop:move=sysop]

2014-03-12T12:32:59Z

Protected "4poy" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:32, 12 March 2014

OCA: New page: '''Unreleased structure''' The entry 4poy is ON HOLD Authors: Fanning, S.W., Walter, R., Horn, J.R. Description: Engineered Dual Specific VHH antibody

2014-03-12T12:32:58Z

New page: '''Unreleased structure''' The entry 4poy is ON HOLD Authors: Fanning, S.W., Walter, R., Horn, J.R. Description: Engineered Dual Specific VHH antibody

New page

'''Unreleased structure'''

The entry 4poy is ON HOLD

Authors: Fanning, S.W., Walter, R., Horn, J.R.

Description: Engineered Dual Specific VHH antibody