4pro - Revision history

OCA at 06:50, 9 August 2023

2023-08-09T06:50:50Z

←Older revision		Revision as of 06:50, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='4pro' size='340' side='right'caption='[[4pro]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4pro' size='340' side='right'caption='[[4pro]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pro]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_29487 Atcc 29487~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PRO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PRO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pro]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PRO FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~T7 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=69 ATCC 29487])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pro OCA], [~~http~~://pdbe.org/4pro PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4pro RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4pro PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4pro ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pro OCA], [https://pdbe.org/4pro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pro RCSB], [https://www.ebi.ac.uk/pdbsum/4pro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pro ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PRLA_LYSEN PRLA_LYSEN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 29487]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Agard, D A~~]]	+	[[Category: Lysobacter enzymogenes]]
-	[[Category: ~~Mau, T~~]]	+	[[Category: Agard DA]]
-	[[Category: ~~Rader, S D~~]]	+	[[Category: Mau T]]
-	[[Category: ~~Sauter, N K~~]]	+	[[Category: Rader SD]]
-	[[Category: ~~Foldase]]~~	+	[[Category: Sauter NK]]
-	~~[[Category: Pro region]]~~	+
-	~~[[Category: Protein folding]]~~	+
-	~~[[Category: Serine protease~~]]	+

OCA at 16:28, 22 January 2020

2020-01-22T16:28:15Z

←Older revision		Revision as of 16:28, 22 January 2020
Line 1:		Line 1:
		+
	==ALPHA-LYTIC PROTEASE COMPLEXED WITH PRO REGION==		==ALPHA-LYTIC PROTEASE COMPLEXED WITH PRO REGION==
-	<StructureSection load='4pro' size='340' side='right' caption='[[4pro]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='4pro' size='340' side='right'caption='[[4pro]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pro]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PRO FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pro]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PRO FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 ATCC 29487])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 ATCC 29487])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pro OCA], [http://pdbe.org/4pro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pro RCSB], [http://www.ebi.ac.uk/pdbsum/4pro PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pro OCA], [http://pdbe.org/4pro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pro RCSB], [http://www.ebi.ac.uk/pdbsum/4pro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pro ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/4pro_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/4pro_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 28:

	==See Also==		==See Also==
-	*[[Alpha-lytic protease\|Alpha-lytic protease]]	+	*[[Alpha-lytic protease 3D structures\|Alpha-lytic protease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 29487]]		[[Category: Atcc 29487]]
		+	[[Category: Large Structures]]
	[[Category: Agard, D A]]		[[Category: Agard, D A]]
	[[Category: Mau, T]]		[[Category: Mau, T]]

OCA at 20:54, 8 February 2016

2016-02-08T20:54:27Z

←Older revision		Revision as of 20:54, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='4pro' size='340' side='right' caption='[[4pro]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4pro' size='340' side='right' caption='[[4pro]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pro]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lysobacter_enzymogenes Lysobacter enzymogenes~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PRO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pro]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29487 Atcc 29487]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PRO FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 ~~Lysobacter enzymogenes~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 ATCC 29487])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pro OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pro RCSB], [http://www.ebi.ac.uk/pdbsum/4pro PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pro OCA], [http://pdbe.org/4pro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pro RCSB], [http://www.ebi.ac.uk/pdbsum/4pro PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4pro ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4pro" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lysobacter enzymogenes~~]]	+	[[Category: Atcc 29487]]
	[[Category: Agard, D A]]		[[Category: Agard, D A]]
	[[Category: Mau, T]]		[[Category: Mau, T]]

OCA at 09:06, 5 January 2015

2015-01-05T09:06:09Z

←Older revision		Revision as of 09:06, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pro]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PRO FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pro]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PRO FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 Lysobacter enzymogenes])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 Lysobacter enzymogenes])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pro OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pro RCSB], [http://www.ebi.ac.uk/pdbsum/4pro PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pro OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pro RCSB], [http://www.ebi.ac.uk/pdbsum/4pro PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Lysobacter enzymogenes]]		[[Category: Lysobacter enzymogenes]]
-	[[Category: Agard, D A.]]	+	[[Category: Agard, D A]]
-	[[Category: Mau, T.]]	+	[[Category: Mau, T]]
-	[[Category: Rader, S D.]]	+	[[Category: Rader, S D]]
-	[[Category: Sauter, N K.]]	+	[[Category: Sauter, N K]]
	[[Category: Foldase]]		[[Category: Foldase]]
	[[Category: Pro region]]		[[Category: Pro region]]
	[[Category: Protein folding]]		[[Category: Protein folding]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

OCA: Protected "4pro" [edit=sysop:move=sysop]

2014-06-05T08:29:51Z

Protected "4pro" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:29, 5 June 2014

OCA at 08:29, 5 June 2014

2014-06-05T08:29:50Z

←Older revision		Revision as of 08:29, 5 June 2014
Line 1:		Line 1:
-	~~{{Seed}}~~	+	==ALPHA-LYTIC PROTEASE COMPLEXED WITH PRO REGION==
-	[[~~Image~~:4pro.~~png\|left~~\|200px]]	+	<StructureSection load='4pro' size='340' side='right' caption='[[4pro]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4pro]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PRO FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 Lysobacter enzymogenes])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pro OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pro RCSB], [http://www.ebi.ac.uk/pdbsum/4pro PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/4pro_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	While the majority of proteins fold rapidly and spontaneously to their native states, the extracellular bacterial protease alpha-lytic protease (alphaLP) has a t(1/2) for folding of approximately 2,000 years, corresponding to a folding barrier of 30 kcal mol(-1). AlphaLP is synthesized as a pro-enzyme where its pro region (Pro) acts as a foldase to stabilize the transition state for the folding reaction. Pro also functions as a potent folding catalyst when supplied as a separate polypeptide chain, accelerating the rate of alphaLP folding by a factor of 3 x 10(9). In the absence of Pro, alphaLP folds only partially to a stable molten globule-like intermediate state. Addition of Pro to this intermediate leads to rapid formation of native alphaLP. Here we report the crystal structures of Pro and of the non-covalent inhibitory complex between Pro and native alphaLP. The C-shaped Pro surrounds the C-terminal beta-barrel domain of the folded protease, forming a large complementary interface. Regions of extensive hydration in the interface explain how Pro binds tightly to the native state, yet even more tightly to the folding transition state. Based on structural and functional data we propose that a specific structural element in alphaLP is largely responsible for the folding barrier and suggest how Pro can overcome this barrier.

-	<!--	+	Structure of alpha-lytic protease complexed with its pro region.,Sauter NK, Mau T, Rader SD, Agard DA Nat Struct Biol. 1998 Nov;5(11):945-50. PMID:9808037<ref>PMID:9808037</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_4pro"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_4pro\| PDB=4pro \| SCENE= }}~~	+

-	~~===ALPHA-LYTIC PROTEASE COMPLEXED WITH PRO REGION===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	~~<!--~~	+	*[[Alpha-lytic protease\|Alpha-lytic protease]]
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_9808037}}, adds the Publication Abstract to the page~~	+	== References ==
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 9808037 is the PubMed ID number.~~	+	<references/>
-	~~-->~~	+	__TOC__
-	~~{{ABSTRACT_PUBMED_9808037}}~~	+	</StructureSection>
-		+
-	==~~About this Structure~~==	+
-	~~4PRO is a 4 chains structure of sequences from~~ [~~http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from~~ [~~http://oca.weizmann.ac.il/oca~~-~~bin/ocashort?id=4PRO OCA~~].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:9808037<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Lysobacter enzymogenes]]		[[Category: Lysobacter enzymogenes]]
	[[Category: Agard, D A.]]		[[Category: Agard, D A.]]
Line 33:		Line 40:
	[[Category: Protein folding]]		[[Category: Protein folding]]
	[[Category: Serine protease]]		[[Category: Serine protease]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 03:03:52 2009''

OCA at 01:03, 17 February 2009

2009-02-17T01:03:57Z

←Older revision		Revision as of 01:03, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	4PRO is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PRO OCA].	+	4PRO is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Lysobacter_enzymogenes Lysobacter enzymogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PRO OCA].

	==Reference==		==Reference==
-	~~Structure of alpha-lytic protease complexed with its pro region., Sauter NK, Mau T, Rader SD, Agard DA, Nat Struct Biol. 1998 Nov;5(11):945-50.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/9808037 9808037]~~	+	<ref group="xtra">PMID:9808037</ref><references group="xtra"/>
	[[Category: Lysobacter enzymogenes]]		[[Category: Lysobacter enzymogenes]]
-	[[Category: Protein complex]]
	[[Category: Agard, D A.]]		[[Category: Agard, D A.]]
	[[Category: Mau, T.]]		[[Category: Mau, T.]]
Line 35:		Line 34:
	[[Category: Serine protease]]		[[Category: Serine protease]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Jul 3 13~~:48:~~00 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 03:03:52 2009''

OCA at 10:48, 3 July 2008

2008-07-03T10:48:00Z

←Older revision		Revision as of 10:48, 3 July 2008
Line 1:		Line 1:
-	[[Image:4pro.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:4pro.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_4pro\| PDB=4pro \| SCENE= }}		{{STRUCTURE_4pro\| PDB=4pro \| SCENE= }}

-	~~'''~~ALPHA-LYTIC PROTEASE COMPLEXED WITH PRO REGION~~'''~~	+	===ALPHA-LYTIC PROTEASE COMPLEXED WITH PRO REGION===


-	~~==Overview==~~	+	<!--
-	~~While~~ the ~~majority of proteins fold rapidly and spontaneously~~ to ~~their native states,~~ the ~~extracellular bacterial protease alpha-lytic protease~~ (~~alphaLP) has a t(1~~/~~2) for folding of approximately 2,000 years, corresponding to a folding barrier of 30 kcal mol(-1)~~. ~~AlphaLP is synthesized as a pro-enzyme where its pro region (Pro) acts as a foldase to stabilize the transition state for the folding reaction~~. ~~Pro also functions as a potent folding catalyst when supplied as a separate polypeptide chain, accelerating the rate of alphaLP folding by a factor of 3 x 10(9~~)~~. In the absence of Pro~~, ~~alphaLP folds only partially to a stable molten globule-like intermediate state. Addition of Pro to this intermediate leads to rapid formation of native alphaLP. Here we report~~ the ~~crystal structures of Pro and of the non-covalent inhibitory complex between Pro and native alphaLP~~. ~~The C~~-~~shaped Pro surrounds the C~~-terminal beta-barrel domain of the folded protease, forming a large complementary interface. Regions of extensive hydration in the interface explain how Pro binds tightly to the native state, yet even more tightly to the folding transition state. Based on structural and functional data we propose that a specific structural element in alphaLP is largely responsible for the folding barrier and suggest how Pro can overcome this barrier.	+	The line below this paragraph, {{ABSTRACT_PUBMED_9808037}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9808037 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9808037}}

	==About this Structure==		==About this Structure==
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	[[Category: Protein folding]]		[[Category: Protein folding]]
	[[Category: Serine protease]]		[[Category: Serine protease]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May~~ ~~4 22~~:28:22 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:48:00 2008''

OCA at 19:28, 4 May 2008

2008-05-04T19:28:26Z

←Older revision		Revision as of 19:28, 4 May 2008
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	[[Image:4pro.gif\|left\|200px]]		[[Image:4pro.gif\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 4pro \|SIZE=350\|CAPTION= <scene name='initialview01'>4pro</scene>~~, ~~resolution 2~~.~~4Å~~	+	The line below this paragraph, containing "STRUCTURE_4pro", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND=~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY=~~	+	or leave the SCENE parameter empty for the default display.
-	~~\|GENE= T7~~ (~~[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 Lysobacter enzymogenes]~~)	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_4pro\| PDB=4pro \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	\|~~RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol~~=4pro ~~FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id~~=~~4pro OCA], [http://www.ebi.ac.uk/pdbsum/4pro PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=4pro RCSB]</span>~~	+
-	}}	+

	'''ALPHA-LYTIC PROTEASE COMPLEXED WITH PRO REGION'''		'''ALPHA-LYTIC PROTEASE COMPLEXED WITH PRO REGION'''
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	[[Category: Rader, S D.]]		[[Category: Rader, S D.]]
	[[Category: Sauter, N K.]]		[[Category: Sauter, N K.]]
-	[[Category: ~~foldase~~]]	+	[[Category: Foldase]]
-	[[Category: ~~pro~~ region]]	+	[[Category: Pro region]]
-	[[Category: ~~protein~~ folding]]	+	[[Category: Protein folding]]
-	[[Category: ~~serine~~ protease]]	+	[[Category: Serine protease]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:28:22 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 05~~:39:52 2008''	+

OCA at 02:39, 31 March 2008

2008-03-31T02:39:54Z

←Older revision		Revision as of 02:39, 31 March 2008
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	\|ACTIVITY=		\|ACTIVITY=
	\|GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 Lysobacter enzymogenes])		\|GENE= T7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69 Lysobacter enzymogenes])
		+	\|DOMAIN=
		+	\|RELATEDENTRY=
		+	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pro OCA], [http://www.ebi.ac.uk/pdbsum/4pro PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=4pro RCSB]</span>
	}}		}}

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	[[Category: serine protease]]		[[Category: serine protease]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Mar ~~20 19~~:10:44 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:39:52 2008''