4pwx - Revision history

OCA at 17:23, 20 September 2023

2023-09-20T17:23:56Z

←Older revision		Revision as of 17:23, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pwx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PWX FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pwx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PWX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 5.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pwx OCA], [https://pdbe.org/4pwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pwx RCSB], [https://www.ebi.ac.uk/pdbsum/4pwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pwx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pwx OCA], [https://pdbe.org/4pwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pwx RCSB], [https://www.ebi.ac.uk/pdbsum/4pwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pwx ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:35, 8 February 2023

2023-02-08T07:35:36Z

←Older revision		Revision as of 07:35, 8 February 2023
Line 1:		Line 1:

	==Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae==		==Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae==
-	<StructureSection load='4pwx' size='340' side='right' caption='[[4pwx]], [[Resolution\|resolution]] 5.40Å' scene=''>	+	<StructureSection load='4pwx' size='340' side='right'caption='[[4pwx]], [[Resolution\|resolution]] 5.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4pwx]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PWX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4PWX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4pwx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PWX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARR4, D2371, GET3, Get3 YDL100C, YDL100C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GET4, GET4 YOR164C, O3580, YOR164C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GET5, GET5 MDY2 YOL111C, MDY2, TMA24, YOL111C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pwx OCA], [https://pdbe.org/4pwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pwx RCSB], [https://www.ebi.ac.uk/pdbsum/4pwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pwx ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4pwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pwx OCA], [~~http~~://pdbe.org/4pwx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4pwx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4pwx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4pwx ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GET3_YEAST GET3_YEAST]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.<ref>PMID:12680698</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21866104</ref> <ref>PMID:21719644</ref> [[http://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref> [[http://www.uniprot.org/uniprot/GET4_YEAST GET4_YEAST]] May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane.<ref>PMID:19325107</ref>	+	[https://www.uniprot.org/uniprot/GET3_YEAST GET3_YEAST] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.<ref>PMID:12680698</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21866104</ref> <ref>PMID:21719644</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4pwx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4pwx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[ATPase 3D structures\|ATPase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Baker's yeast~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Clemons, W M~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Gristick, H B~~]]	+	[[Category: Clemons Jr WM]]
-	[[Category: ~~Tail-anchored targeting]]~~	+	[[Category: Gristick HB]]
-	~~[[Category: Transport protein~~]]	+

OCA at 08:46, 22 November 2017

2017-11-22T08:46:21Z

←Older revision		Revision as of 08:46, 22 November 2017
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4pwx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4pwx" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[ATPase\|ATPase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:49, 11 August 2016

2016-08-11T19:49:47Z

←Older revision		Revision as of 19:49, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae==		==Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae==
	<StructureSection load='4pwx' size='340' side='right' caption='[[4pwx]], [[Resolution\|resolution]] 5.40Å' scene=''>		<StructureSection load='4pwx' size='340' side='right' caption='[[4pwx]], [[Resolution\|resolution]] 5.40Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARR4, D2371, GET3, Get3 YDL100C, YDL100C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GET4, GET4 YOR164C, O3580, YOR164C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GET5, GET5 MDY2 YOL111C, MDY2, TMA24, YOL111C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARR4, D2371, GET3, Get3 YDL100C, YDL100C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GET4, GET4 YOR164C, O3580, YOR164C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GET5, GET5 MDY2 YOL111C, MDY2, TMA24, YOL111C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pwx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pwx RCSB], [http://www.ebi.ac.uk/pdbsum/4pwx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pwx OCA], [http://pdbe.org/4pwx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pwx RCSB], [http://www.ebi.ac.uk/pdbsum/4pwx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pwx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4pwx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[ATPase\|ATPase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:41, 5 January 2015

2015-01-05T08:41:46Z

←Older revision		Revision as of 08:41, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4pwx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PWX FirstGlance]. <br>		<table><tr><td colspan='2'>[[4pwx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PWX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARR4, D2371, GET3, Get3 YDL100C, YDL100C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GET4, GET4 YOR164C, O3580, YOR164C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GET5, GET5 MDY2 YOL111C, MDY2, TMA24, YOL111C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARR4, D2371, GET3, Get3 YDL100C, YDL100C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GET4, GET4 YOR164C, O3580, YOR164C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GET5, GET5 MDY2 YOL111C, MDY2, TMA24, YOL111C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pwx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pwx RCSB], [http://www.ebi.ac.uk/pdbsum/4pwx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pwx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pwx RCSB], [http://www.ebi.ac.uk/pdbsum/4pwx PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GET3_YEAST GET3_YEAST]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.<ref>PMID:12680698</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21866104</ref> <ref>PMID:21719644</ref> [[http://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref> [[http://www.uniprot.org/uniprot/GET4_YEAST GET4_YEAST]] May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane.<ref>PMID:19325107</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 15:
	Crystal structure of ATP-bound Get3-Get4-Get5 complex reveals regulation of Get3 by Get4.,Gristick HB, Rao M, Chartron JW, Rome ME, Shan SO, Clemons WM Jr Nat Struct Mol Biol. 2014 May;21(5):437-42. doi: 10.1038/nsmb.2813. Epub 2014 Apr, 13. PMID:24727835<ref>PMID:24727835</ref>		Crystal structure of ATP-bound Get3-Get4-Get5 complex reveals regulation of Get3 by Get4.,Gristick HB, Rao M, Chartron JW, Rome ME, Shan SO, Clemons WM Jr Nat Struct Mol Biol. 2014 May;21(5):437-42. doi: 10.1038/nsmb.2813. Epub 2014 Apr, 13. PMID:24727835<ref>PMID:24727835</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
-	[[Category: Clemons, W M.]]	+	[[Category: Clemons, W M]]
-	[[Category: Gristick, H B.]]	+	[[Category: Gristick, H B]]
	[[Category: Tail-anchored targeting]]		[[Category: Tail-anchored targeting]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 06:50, 21 May 2014

2014-05-21T06:50:45Z

←Older revision		Revision as of 06:50, 21 May 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4pwx\| PDB=4pwx \| SCENE= }}~~	+	==Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae==
-	===Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae===	+	<StructureSection load='4pwx' size='340' side='right' caption='[[4pwx]], [[Resolution\|resolution]] 5.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4pwx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PWX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARR4, D2371, GET3, Get3 YDL100C, YDL100C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GET4, GET4 YOR164C, O3580, YOR164C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), GET5, GET5 MDY2 YOL111C, MDY2, TMA24, YOL111C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pwx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pwx RCSB], [http://www.ebi.ac.uk/pdbsum/4pwx PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Correct localization of membrane proteins is essential to all cells. Chaperone cascades coordinate the capture and handover of substrate proteins from the ribosomes to the target membranes, yet the mechanistic and structural details of these processes remain unclear. Here we investigate the conserved GET pathway, in which the Get4-Get5 complex mediates the handover of tail-anchor (TA) substrates from the cochaperone Sgt2 to the Get3 ATPase, the central targeting factor. We present a crystal structure of a yeast Get3-Get4-Get5 complex in an ATP-bound state and show how Get4 primes Get3 by promoting the optimal configuration for substrate capture. Structure-guided biochemical analyses demonstrate that Get4-mediated regulation of ATP hydrolysis by Get3 is essential to efficient TA-protein targeting. Analogous regulation of other chaperones or targeting factors could provide a general mechanism for ensuring effective substrate capture during protein biogenesis.

-	~~==Function==~~	+	Crystal structure of ATP-bound Get3-Get4-Get5 complex reveals regulation of Get3 by Get4.,Gristick HB, Rao M, Chartron JW, Rome ME, Shan SO, Clemons WM Jr Nat Struct Mol Biol. 2014 May;21(5):437-42. doi: 10.1038/nsmb.2813. Epub 2014 Apr, 13. PMID:24727835<ref>PMID:24727835</ref>
-	~~[[http://www.uniprot.org/uniprot/GET3_YEAST GET3_YEAST]] ATPase required for the post-translational delivery~~ of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound ~~receptors GET1 and GET2, where the tail~~-~~anchored protein is released for insertion. This process is regulated~~ by ~~ATP binding and hydrolysis~~. ~~ATP binding drives the homodimer towards the closed dimer state~~, ~~facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently~~, ~~the homodimer reverts towards the open dimer state~~, ~~lowering its affinity for the GET1-GET2 receptor~~, ~~and returning it to the cytosol to initiate a new round of targeting~~. ~~Cooperates with the HDEL receptor ERD2 to mediate the ATP~~-~~dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER~~. ~~Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance~~.~~<ref>PMID:12680698</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21866104</ref> <ref>PMID:21719644</ref> [[http:/~~/~~www~~.~~uniprot~~.~~org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor~~, ~~the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos~~.~~<ref>~~PMID:~~10514570</ref> <ref>PMID:16390866</ref> [[http://www.uniprot.org/uniprot/GET4_YEAST GET4_YEAST]] May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane.~~<ref>PMID:~~19325107~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4pwx]] is~~ a ~~6 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4PWX OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
		+	[[Category: Baker's yeast]]
	[[Category: Clemons, W M.]]		[[Category: Clemons, W M.]]
	[[Category: Gristick, H B.]]		[[Category: Gristick, H B.]]
	[[Category: Tail-anchored targeting]]		[[Category: Tail-anchored targeting]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 10:16, 9 April 2014

2014-04-09T10:16:27Z

←Older revision		Revision as of 10:16, 9 April 2014
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-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4pwx\| PDB=4pwx \| SCENE= }}
		+	===Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae===

-	~~The entry 4pwx~~ is ~~ON HOLD~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/GET3_YEAST GET3_YEAST]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors GET1 and GET2, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the GET1-GET2 receptor, and returning it to the cytosol to initiate a new round of targeting. Cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER. Involved in low-level resistance to the oxyanions arsenite and arsenate, and in heat tolerance.<ref>PMID:12680698</ref> <ref>PMID:16269340</ref> <ref>PMID:18724936</ref> <ref>PMID:21866104</ref> <ref>PMID:21719644</ref> [[http://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref> [[http://www.uniprot.org/uniprot/GET4_YEAST GET4_YEAST]] May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane.<ref>PMID:19325107</ref>

-	~~Authors: Gristick, H~~.G.~~, Clemons Jr~~.~~, W~~.M.	+	==About this Structure==
		+	[[4pwx]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PWX OCA].

-	~~Description~~: ~~Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S~~.~~cerevisiae~~	+	==Reference==
		+	<references group="xtra"/><references/>
		+	[[Category: Clemons, W M.]]
		+	[[Category: Gristick, H B.]]
		+	[[Category: Tail-anchored targeting]]
		+	[[Category: Transport protein]]

OCA: Protected "4pwx" [edit=sysop:move=sysop]

2014-04-02T08:34:25Z

Protected "4pwx" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:34, 2 April 2014

OCA: New page: '''Unreleased structure''' The entry 4pwx is ON HOLD Authors: Gristick, H.G., Clemons Jr., W.M. Description: Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae

2014-04-02T08:34:25Z

New page: '''Unreleased structure''' The entry 4pwx is ON HOLD Authors: Gristick, H.G., Clemons Jr., W.M. Description: Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae

New page

'''Unreleased structure'''

The entry 4pwx is ON HOLD

Authors: Gristick, H.G., Clemons Jr., W.M.

Description: Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae