4q4j - Revision history

OCA at 07:43, 8 February 2023

2023-02-08T07:43:26Z

←Older revision		Revision as of 07:43, 8 February 2023
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	==Structure of crosslinked TM287/288_S498C_S520C mutant==		==Structure of crosslinked TM287/288_S498C_S520C mutant==
-	<StructureSection load='4q4j' size='340' side='right' caption='[[4q4j]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='4q4j' size='340' side='right'caption='[[4q4j]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4q4j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Thema Thema~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q4J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4Q4J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4q4j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q4J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q4J FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q4h\|4q4h]], [[4q4a\|4q4a]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q4j OCA], [https://pdbe.org/4q4j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q4j RCSB], [https://www.ebi.ac.uk/pdbsum/4q4j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q4j ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM_0287, THEMA_03290, Tmari_0285 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA]), TM_0288 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4q4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q4j OCA], [~~http~~://pdbe.org/4q4j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4q4j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4q4j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4q4j ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9WYC3_THEMA Q9WYC3_THEMA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thema~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Gruetter, M G~~]]	+	[[Category: Thermotoga maritima MSB8]]
-	[[Category: ~~Hohl, M~~]]	+	[[Category: Gruetter MG]]
-	[[Category: ~~Schoeppe, J]]~~	+	[[Category: Hohl M]]
-	~~[[Category: Seeger,~~ M A]]	+	[[Category: Schoeppe J]]
-	[[Category: ~~Abc exporter~~]]	+	[[Category: Seeger MA]]
-	[[Category: ~~Abc transporter]]~~	+
-	~~[[Category: Hydrolase-transport protein complex]]~~	+
-	~~[[Category: Membrane transporter]]~~	+
-	~~[[Category: Multidrug efflux~~]]	+

OCA at 08:48, 22 November 2017

2017-11-22T08:48:53Z

←Older revision		Revision as of 08:48, 22 November 2017
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	<StructureSection load='4q4j' size='340' side='right' caption='[[4q4j]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='4q4j' size='340' side='right' caption='[[4q4j]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4q4j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q4J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4q4j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q4J FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q4h\|4q4h]], [[4q4a\|4q4a]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q4h\|4q4h]], [[4q4a\|4q4a]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM_0287, THEMA_03290, Tmari_0285 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA]), TM_0288 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q4j OCA], [http://pdbe.org/4q4j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q4j RCSB], [http://www.ebi.ac.uk/pdbsum/4q4j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q4j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q4j OCA], [http://pdbe.org/4q4j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q4j RCSB], [http://www.ebi.ac.uk/pdbsum/4q4j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q4j ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Thema]]
	[[Category: Gruetter, M G]]		[[Category: Gruetter, M G]]
	[[Category: Hohl, M]]		[[Category: Hohl, M]]

OCA at 20:52, 11 August 2016

2016-08-11T20:52:13Z

←Older revision		Revision as of 20:52, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of crosslinked TM287/288_S498C_S520C mutant==		==Structure of crosslinked TM287/288_S498C_S520C mutant==
	<StructureSection load='4q4j' size='340' side='right' caption='[[4q4j]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='4q4j' size='340' side='right' caption='[[4q4j]], [[Resolution\|resolution]] 3.20Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4q4j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q4J FirstGlance]. <br>		<table><tr><td colspan='2'>[[4q4j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q4J FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q4h\|4q4h]], [[4q4a\|4q4a]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q4h\|4q4h]], [[4q4a\|4q4a]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q4j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q4j RCSB], [http://www.ebi.ac.uk/pdbsum/4q4j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q4j OCA], [http://pdbe.org/4q4j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4q4j RCSB], [http://www.ebi.ac.uk/pdbsum/4q4j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4q4j ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4q4j" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:35, 5 January 2015

2015-01-05T14:35:11Z

←Older revision		Revision as of 14:35, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4q4j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q4J FirstGlance]. <br>		<table><tr><td colspan='2'>[[4q4j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q4J FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q4h\|4q4h]], [[4q4a\|4q4a]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q4h\|4q4h]], [[4q4a\|4q4a]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q4j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q4j RCSB], [http://www.ebi.ac.uk/pdbsum/4q4j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q4j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q4j RCSB], [http://www.ebi.ac.uk/pdbsum/4q4j PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Gruetter, M G.]]	+	[[Category: Gruetter, M G]]
-	[[Category: Hohl, M.]]	+	[[Category: Hohl, M]]
-	[[Category: Schoeppe, J.]]	+	[[Category: Schoeppe, J]]
-	[[Category: Seeger, M A.]]	+	[[Category: Seeger, M A]]
	[[Category: Abc exporter]]		[[Category: Abc exporter]]
	[[Category: Abc transporter]]		[[Category: Abc transporter]]

OCA at 13:43, 20 August 2014

2014-08-20T13:43:24Z

←Older revision		Revision as of 13:43, 20 August 2014
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	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q4j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q4j RCSB], [http://www.ebi.ac.uk/pdbsum/4q4j PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q4j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q4j RCSB], [http://www.ebi.ac.uk/pdbsum/4q4j PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ATP binding cassette (ABC) transporters mediate vital transport processes in every living cell. ATP hydrolysis, which fuels transport, displays positive cooperativity in numerous ABC transporters. In particular, heterodimeric ABC exporters exhibit pronounced allosteric coupling between a catalytically impaired degenerate site, where nucleotides bind tightly, and a consensus site, at which ATP is hydrolyzed in every transport cycle. Whereas the functional phenomenon of cooperativity is well described, its structural basis remains poorly understood. Here, we present the apo structure of the heterodimeric ABC exporter TM287/288 and compare it to the previously solved structure with adenosine 5'-(beta,gamma-imido)triphosphate (AMP-PNP) bound at the degenerate site. In contrast to other ABC exporter structures, the nucleotide binding domains (NBDs) of TM287/288 remain in molecular contact even in the absence of nucleotides, and the arrangement of the transmembrane domains (TMDs) is not influenced by AMP-PNP binding, a notion confirmed by double electron-electron resonance (DEER) measurements. Nucleotide binding at the degenerate site results in structural rearrangements, which are transmitted to the consensus site via two D-loops located at the NBD interface. These loops owe their name from a highly conserved aspartate and are directly connected to the catalytically important Walker B motif. The D-loop at the degenerate site ties the NBDs together even in the absence of nucleotides and substitution of its aspartate by alanine is well-tolerated. By contrast, the D-loop of the consensus site is flexible and the aspartate to alanine mutation and conformational restriction by cross-linking strongly reduces ATP hydrolysis and substrate transport.
		+
		+	Structural basis for allosteric cross-talk between the asymmetric nucleotide binding sites of a heterodimeric ABC exporter.,Hohl M, Hurlimann LM, Bohm S, Schoppe J, Grutter MG, Bordignon E, Seeger MA Proc Natl Acad Sci U S A. 2014 Jul 29;111(30):11025-30. doi:, 10.1073/pnas.1400485111. Epub 2014 Jul 16. PMID:25030449<ref>PMID:25030449</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:43, 23 July 2014

2014-07-23T06:43:58Z

←Older revision		Revision as of 06:43, 23 July 2014
Line 14:		Line 14:
	[[Category: Abc exporter]]		[[Category: Abc exporter]]
	[[Category: Abc transporter]]		[[Category: Abc transporter]]
		+	[[Category: Hydrolase-transport protein complex]]
	[[Category: Membrane transporter]]		[[Category: Membrane transporter]]
	[[Category: Multidrug efflux]]		[[Category: Multidrug efflux]]
-	[[Category: Transport protein]]

OCA at 10:33, 16 July 2014

2014-07-16T10:33:13Z

←Older revision		Revision as of 10:33, 16 July 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Structure of crosslinked TM287/288_S498C_S520C mutant==
-		+	<StructureSection load='4q4j' size='340' side='right' caption='[[4q4j]], [[Resolution\|resolution]] 3.20Å' scene=''>
-	~~The entry~~ 4q4j is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4q4j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Q4J FirstGlance]. <br>
-	~~Authors~~: ~~Hohl~~, M., ~~Schoeppe, J~~., Gruetter, M.G., Seeger, M.A.	+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4q4h\|4q4h]], [[4q4a\|4q4a]]</td></tr>
-		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4q4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q4j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4q4j RCSB], [http://www.ebi.ac.uk/pdbsum/4q4j PDBsum]</span></td></tr>
-	~~Description~~:	+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gruetter, M G.]]
		+	[[Category: Hohl, M.]]
		+	[[Category: Schoeppe, J.]]
		+	[[Category: Seeger, M A.]]
		+	[[Category: Abc exporter]]
		+	[[Category: Abc transporter]]
		+	[[Category: Membrane transporter]]
		+	[[Category: Multidrug efflux]]
		+	[[Category: Transport protein]]

OCA at 08:13, 21 May 2014

2014-05-21T08:13:27Z

←Older revision		Revision as of 08:13, 21 May 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4q4j is ON HOLD	+	The entry 4q4j is ON HOLD until Paper Publication

	Authors: Hohl, M., Schoeppe, J., Gruetter, M.G., Seeger, M.A.		Authors: Hohl, M., Schoeppe, J., Gruetter, M.G., Seeger, M.A.

	Description:		Description:

OCA: Protected "4q4j" [edit=sysop:move=sysop]

2014-04-23T07:29:42Z

Protected "4q4j" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:29, 23 April 2014

OCA: New page: '''Unreleased structure''' The entry 4q4j is ON HOLD Authors: Hohl, M., Schoeppe, J., Gruetter, M.G., Seeger, M.A. Description:

2014-04-23T07:29:41Z

New page: '''Unreleased structure''' The entry 4q4j is ON HOLD Authors: Hohl, M., Schoeppe, J., Gruetter, M.G., Seeger, M.A. Description:

New page

'''Unreleased structure'''

The entry 4q4j is ON HOLD

Authors: Hohl, M., Schoeppe, J., Gruetter, M.G., Seeger, M.A.

Description: