4qpj - Revision history

OCA at 12:47, 1 March 2024

2024-03-01T12:47:49Z

←Older revision		Revision as of 12:47, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4qpj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus_2308 Brucella abortus 2308]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QPJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QPJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4qpj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus_2308 Brucella abortus 2308]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QPJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QPJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.742Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qpj OCA], [https://pdbe.org/4qpj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qpj RCSB], [https://www.ebi.ac.uk/pdbsum/4qpj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qpj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qpj OCA], [https://pdbe.org/4qpj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qpj RCSB], [https://www.ebi.ac.uk/pdbsum/4qpj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qpj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CHPT_BRUA2 CHPT_BRUA2] Component of a regulatory phosphorelay system that controls B.abortus cell growth, division, and intracellular survival inside mammalian host cells. This signaling pathway is composed of CckA, ChpT, CtrA and CpdR. ChpT efficiently and specifically shuttles phosphoryl groups from the CckA kinase to the receiver domains of both CtrA and CpdR. Does not bind ATP. Overexpression of chpT results in a defect in cell morphology, DNA content, and intracellular survival in human macrophages.<ref>PMID:26124143</ref>		[https://www.uniprot.org/uniprot/CHPT_BRUA2 CHPT_BRUA2] Component of a regulatory phosphorelay system that controls B.abortus cell growth, division, and intracellular survival inside mammalian host cells. This signaling pathway is composed of CckA, ChpT, CtrA and CpdR. ChpT efficiently and specifically shuttles phosphoryl groups from the CckA kinase to the receiver domains of both CtrA and CpdR. Does not bind ATP. Overexpression of chpT results in a defect in cell morphology, DNA content, and intracellular survival in human macrophages.<ref>PMID:26124143</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We have functionally and structurally defined an essential protein phosphorelay that regulates expression of genes required for growth, division, and intracellular survival of the global zoonotic pathogen Brucella abortus. Our study delineates phosphoryl transfer through this molecular pathway, which initiates from the sensor kinase CckA and proceeds through the ChpT phosphotransferase to two regulatory substrates: CtrA and CpdR. Genetic perturbation of this system results in defects in cell growth and division site selection, and a specific viability deficit inside human phagocytic cells. Thus, proper control of B. abortus division site polarity is necessary for survival in the intracellular niche. We further define the structural foundations of signaling from the central phosphotransferase, ChpT, to its response regulator substrate, CtrA, and provide evidence that there are at least two modes of interaction between ChpT and CtrA, only one of which is competent to catalyze phosphoryltransfer. The structure and dynamics of the active site on each side of the ChpT homodimer are distinct, supporting a model in which quaternary structure of the 2:2 ChpT-CtrA complex enforces an asymmetric mechanism of phosphoryl transfer between ChpT and CtrA. Our study provides mechanistic understanding, from the cellular to the atomic scale, of a conserved transcriptional regulatory system that controls the cellular and infection biology of B. abortus. More generally, our results provide insight into the structural basis of two-component signal transduction, which is broadly conserved in bacteria, plants, and fungi.
-
-	Structural asymmetry in a conserved signaling system that regulates division, replication, and virulence of an intracellular pathogen.,Willett JW, Herrou J, Briegel A, Rotskoff G, Crosson S Proc Natl Acad Sci U S A. 2015 Jun 29. pii: 201503118. PMID:26124143<ref>PMID:26124143</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4qpj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 13:45, 22 February 2023

2023-02-22T13:45:28Z

←Older revision		Revision as of 13:45, 22 February 2023
Line 1:		Line 1:

	==2.7 Angstrom Structure of a Phosphotransferase in Complex with a Receiver Domain==		==2.7 Angstrom Structure of a Phosphotransferase in Complex with a Receiver Domain==
-	<StructureSection load='4qpj' size='340' side='right' caption='[[4qpj]], [[Resolution\|resolution]] 2.74Å' scene=''>	+	<StructureSection load='4qpj' size='340' side='right'caption='[[4qpj]], [[Resolution\|resolution]] 2.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qpj]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Brua2 Brua2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QPJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4QPJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qpj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus_2308 Brucella abortus 2308]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QPJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QPJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qpk\|4qpk]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qpj OCA], [https://pdbe.org/4qpj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qpj RCSB], [https://www.ebi.ac.uk/pdbsum/4qpj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qpj ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAB1_1613, BruAb1_1585 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=359391 BRUA2]), BAB1_1614, BruAb1_1586, ctrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=359391 BRUA2])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4qpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qpj OCA], [~~http~~://pdbe.org/4qpj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4qpj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4qpj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4qpj ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~CTRA_BRUA2 CTRA_BRUA2~~]~~] Essential protein that plays a role in the control~~ of cell division, ~~possibly through the transcriptional regulation~~ of ~~ccrM~~, ~~rpoD~~, ~~pleC~~, ~~minC~~ and ~~ftsZ genes~~.	+	[https://www.uniprot.org/uniprot/CHPT_BRUA2 CHPT_BRUA2] Component of a regulatory phosphorelay system that controls B.abortus cell growth, division, and intracellular survival inside mammalian host cells. This signaling pathway is composed of CckA, ChpT, CtrA and CpdR. ChpT efficiently and specifically shuttles phosphoryl groups from the CckA kinase to the receiver domains of both CtrA and CpdR. Does not bind ATP. Overexpression of chpT results in a defect in cell morphology, DNA content, and intracellular survival in human macrophages.<ref>PMID:26124143</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
-	*[[Phosphotransferase\|Phosphotransferase]]	+	*[[Phosphotransferase 3D structures\|Phosphotransferase 3D structures]]
		+	*[[Response regulator 3D structure\|Response regulator 3D structure]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Brua2~~]]	+	[[Category: Brucella abortus 2308]]
-	[[Category: ~~Crosson, S~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Herrou, J~~]]	+	[[Category: Crosson S]]
-	[[Category: ~~Willett,~~ J W]]	+	[[Category: Herrou J]]
-	[[Category: ~~Atp-binding]]~~	+	[[Category: Willett JW]]
-	~~[[Category: Catalytic domain]]~~	+
-	~~[[Category: Chpt]]~~	+
-	~~[[Category: Chpt-ctra complex]]~~	+
-	~~[[Category: Ctra]]~~	+
-	~~[[Category: Histidine kinase]]~~	+
-	~~[[Category: Histidine kinase like]]~~	+
-	~~[[Category: Hpt]]~~	+
-	~~[[Category: Phosphotransferase]]~~	+
-	~~[[Category: Response regulator]]~~	+
-	~~[[Category: Rr]]~~	+
-	~~[[Category: Signaling protein-dna binding protein complex~~]]	+

OCA at 08:56, 22 November 2017

2017-11-22T08:56:27Z

←Older revision		Revision as of 08:56, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='4qpj' size='340' side='right' caption='[[4qpj]], [[Resolution\|resolution]] 2.74Å' scene=''>		<StructureSection load='4qpj' size='340' side='right' caption='[[4qpj]], [[Resolution\|resolution]] 2.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qpj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QPJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QPJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qpj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Brua2 Brua2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QPJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QPJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qpk\|4qpk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qpk\|4qpk]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAB1_1613, BruAb1_1585 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=359391 BRUA2]), BAB1_1614, BruAb1_1586, ctrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=359391 BRUA2])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qpj OCA], [http://pdbe.org/4qpj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qpj RCSB], [http://www.ebi.ac.uk/pdbsum/4qpj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qpj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qpj OCA], [http://pdbe.org/4qpj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qpj RCSB], [http://www.ebi.ac.uk/pdbsum/4qpj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qpj ProSAT]</span></td></tr>
	</table>		</table>
Line 19:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4qpj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4qpj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphotransferase\|Phosphotransferase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Brua2]]
	[[Category: Crosson, S]]		[[Category: Crosson, S]]
	[[Category: Herrou, J]]		[[Category: Herrou, J]]

OCA at 13:24, 10 May 2017

2017-05-10T13:24:36Z

←Older revision		Revision as of 13:24, 10 May 2017
Line 1:		Line 1:
		+
	==2.7 Angstrom Structure of a Phosphotransferase in Complex with a Receiver Domain==		==2.7 Angstrom Structure of a Phosphotransferase in Complex with a Receiver Domain==
	<StructureSection load='4qpj' size='340' side='right' caption='[[4qpj]], [[Resolution\|resolution]] 2.74Å' scene=''>		<StructureSection load='4qpj' size='340' side='right' caption='[[4qpj]], [[Resolution\|resolution]] 2.74Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qpk\|4qpk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qpk\|4qpk]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qpj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qpj RCSB], [http://www.ebi.ac.uk/pdbsum/4qpj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qpj OCA], [http://pdbe.org/4qpj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qpj RCSB], [http://www.ebi.ac.uk/pdbsum/4qpj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qpj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4qpj" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:27, 15 July 2015

2015-07-15T13:27:35Z

←Older revision		Revision as of 13:27, 15 July 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==2.7 Angstrom Structure of a Phosphotransferase in Complex with a Receiver Domain==
		+	<StructureSection load='4qpj' size='340' side='right' caption='[[4qpj]], [[Resolution\|resolution]] 2.74Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4qpj]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QPJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QPJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4qpk\|4qpk]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qpj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qpj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qpj RCSB], [http://www.ebi.ac.uk/pdbsum/4qpj PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CTRA_BRUA2 CTRA_BRUA2]] Essential protein that plays a role in the control of cell division, possibly through the transcriptional regulation of ccrM, rpoD, pleC, minC and ftsZ genes.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have functionally and structurally defined an essential protein phosphorelay that regulates expression of genes required for growth, division, and intracellular survival of the global zoonotic pathogen Brucella abortus. Our study delineates phosphoryl transfer through this molecular pathway, which initiates from the sensor kinase CckA and proceeds through the ChpT phosphotransferase to two regulatory substrates: CtrA and CpdR. Genetic perturbation of this system results in defects in cell growth and division site selection, and a specific viability deficit inside human phagocytic cells. Thus, proper control of B. abortus division site polarity is necessary for survival in the intracellular niche. We further define the structural foundations of signaling from the central phosphotransferase, ChpT, to its response regulator substrate, CtrA, and provide evidence that there are at least two modes of interaction between ChpT and CtrA, only one of which is competent to catalyze phosphoryltransfer. The structure and dynamics of the active site on each side of the ChpT homodimer are distinct, supporting a model in which quaternary structure of the 2:2 ChpT-CtrA complex enforces an asymmetric mechanism of phosphoryl transfer between ChpT and CtrA. Our study provides mechanistic understanding, from the cellular to the atomic scale, of a conserved transcriptional regulatory system that controls the cellular and infection biology of B. abortus. More generally, our results provide insight into the structural basis of two-component signal transduction, which is broadly conserved in bacteria, plants, and fungi.

-	~~The entry 4qpj is ON HOLD until Paper Publication~~	+	Structural asymmetry in a conserved signaling system that regulates division, replication, and virulence of an intracellular pathogen.,Willett JW, Herrou J, Briegel A, Rotskoff G, Crosson S Proc Natl Acad Sci U S A. 2015 Jun 29. pii: 201503118. PMID:26124143<ref>PMID:26124143</ref>

-	~~Authors: Willett~~, J.W.~~, Herrou, J., Crosson, S~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: 2.7 Angstrom Structure of a Phosphotransferase in Complex with a Receiver Domain~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
-	[[Category: ~~Willett~~, ~~J.W~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Crosson, S]]
	[[Category: Herrou, J]]		[[Category: Herrou, J]]
-	[[Category: ~~Crosson~~, S]]	+	[[Category: Willett, J W]]
		+	[[Category: Atp-binding]]
		+	[[Category: Catalytic domain]]
		+	[[Category: Chpt]]
		+	[[Category: Chpt-ctra complex]]
		+	[[Category: Ctra]]
		+	[[Category: Histidine kinase]]
		+	[[Category: Histidine kinase like]]
		+	[[Category: Hpt]]
		+	[[Category: Phosphotransferase]]
		+	[[Category: Response regulator]]
		+	[[Category: Rr]]
		+	[[Category: Signaling protein-dna binding protein complex]]

OCA at 16:01, 24 December 2014

2014-12-24T16:01:01Z

←Older revision		Revision as of 16:01, 24 December 2014
Line 6:		Line 6:

	Description: 2.7 Angstrom Structure of a Phosphotransferase in Complex with a Receiver Domain		Description: 2.7 Angstrom Structure of a Phosphotransferase in Complex with a Receiver Domain
		+	[[Category: Unreleased Structures]]
		+	[[Category: Willett, J.W]]
		+	[[Category: Herrou, J]]
		+	[[Category: Crosson, S]]

OCA: Protected "4qpj" [edit=sysop:move=sysop]

2014-07-02T07:27:44Z

Protected "4qpj" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:27, 2 July 2014

OCA: New page: '''Unreleased structure''' The entry 4qpj is ON HOLD until Paper Publication Authors: Willett, J.W., Herrou, J., Crosson, S. Description: 2.7 Angstrom Structure of a Phosphotransferase...

2014-07-02T07:27:43Z

New page: '''Unreleased structure''' The entry 4qpj is ON HOLD until Paper Publication Authors: Willett, J.W., Herrou, J., Crosson, S. Description: 2.7 Angstrom Structure of a Phosphotransferase...

New page

'''Unreleased structure'''

The entry 4qpj is ON HOLD until Paper Publication

Authors: Willett, J.W., Herrou, J., Crosson, S.

Description: 2.7 Angstrom Structure of a Phosphotransferase in Complex with a Receiver Domain