4qqu - Revision history

OCA at 06:52, 3 April 2024

2024-04-03T06:52:14Z

←Older revision		Revision as of 06:52, 3 April 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/METE_CANAL METE_CANAL] Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.[HAMAP-Rule:MF_00172]		[https://www.uniprot.org/uniprot/METE_CANAL METE_CANAL] Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.[HAMAP-Rule:MF_00172]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The cobalamin-independent methionine synthase enzyme catalyzes a challenging reaction: the direct transfer of a methyl from 5-methyl-tetrahydrofolate-glutamate3 to the l-homocysteine thiol. The enzyme has a dual (betaalpha)8 TIM barrel structure that binds, activates and brings the reactants into reaction proximity by conformational movements. In the previously observed open structures, the substrates bind too far apart to react, but we have captured a ternary complex with both substrates bound in a closed form of the enzyme. The closing is described in terms of a hinge between the N- and C-terminal TIM barrels and a rearrangement of key loops within the C domain. The substrate specificity can now be rationalized and the structure reveals His707 as the acid that protonates the THF leaving group through a water molecule trapped in the closed active site. The substrates are correctly oriented for an in-line attack by l-homocysteine on the N5-methyl.
-
-	The Cobalamin-Independent Methionine Synthase Enzyme Captured in a Substrate-Induced Closed Conformation.,Ubhi DK, Robertus JD J Mol Biol. 2014 Dec 27. pii: S0022-2836(14)00649-4. doi:, 10.1016/j.jmb.2014.12.014. PMID:25545590<ref>PMID:25545590</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4qqu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Methionine synthase 3D structures\|Methionine synthase 3D structures]]		*[[Methionine synthase 3D structures\|Methionine synthase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:35, 20 September 2023

2023-09-20T17:35:50Z

←Older revision		Revision as of 17:35, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4qqu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QQU FirstGlance]. <br>		<table><tr><td colspan='2'>[[4qqu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QQU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=39S:N-[4-({[(6S)-2-AMINO-4-OXO-3,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GAMMA-GLUTAMYL-L-GAMMA-GLUTAMYL-L-GLUTAMIC+ACID'>39S</scene>, <scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.98Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=39S:N-[4-({[(6S)-2-AMINO-4-OXO-3,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GAMMA-GLUTAMYL-L-GAMMA-GLUTAMYL-L-GLUTAMIC+ACID'>39S</scene>, <scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqu OCA], [https://pdbe.org/4qqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qqu RCSB], [https://www.ebi.ac.uk/pdbsum/4qqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqu OCA], [https://pdbe.org/4qqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qqu RCSB], [https://www.ebi.ac.uk/pdbsum/4qqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqu ProSAT]</span></td></tr>
	</table>		</table>
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4qqu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4qqu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Methionine synthase 3D structures\|Methionine synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:32, 30 September 2022

2022-09-30T04:32:05Z

←Older revision		Revision as of 04:32, 30 September 2022
Line 3:		Line 3:
	<StructureSection load='4qqu' size='340' side='right'caption='[[4qqu]], [[Resolution\|resolution]] 2.98Å' scene=''>		<StructureSection load='4qqu' size='340' side='right'caption='[[4qqu]], [[Resolution\|resolution]] 2.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qqu]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_11006_[[candida_stellatoidea]] Atcc 11006 [[candida stellatoidea]]~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4QQU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qqu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QQU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=39S:N-[4-({[(6S)-2-AMINO-4-OXO-3,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GAMMA-GLUTAMYL-L-GAMMA-GLUTAMYL-L-GLUTAMIC+ACID'>39S</scene>, <scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=39S:N-[4-({[(6S)-2-AMINO-4-OXO-3,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GAMMA-GLUTAMYL-L-GAMMA-GLUTAMYL-L-GLUTAMIC+ACID'>39S</scene>, <scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CaO19.10083, CaO19.2551, MET6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[Candida stellatoidea]]])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqu OCA], [https://pdbe.org/4qqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qqu RCSB], [https://www.ebi.ac.uk/pdbsum/4qqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqu ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4qqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqu OCA], [~~http~~://pdbe.org/4qqu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4qqu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4qqu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqu ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/METE_CANAL METE_CANAL]] Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.[HAMAP-Rule:MF_00172]	+	[https://www.uniprot.org/uniprot/METE_CANAL METE_CANAL] Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.[HAMAP-Rule:MF_00172]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase~~]]	+	[[Category: Candida albicans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Robertus~~, J D~~]]	+	[[Category: Robertus JD]]
-	[[Category: Ubhi~~, D K]]~~	+	[[Category: Ubhi DK]]
-	~~[[Category: Closed conformation]]~~	+
-	~~[[Category: Cobalamin-independent]]~~	+
-	~~[[Category: Dual tim barrel]]~~	+
-	~~[[Category: Fungal]]~~	+
-	~~[[Category: Methionine synthase]]~~	+
-	~~[[Category: Surface entropy reduction]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:49, 24 April 2019

2019-04-24T08:49:06Z

←Older revision		Revision as of 08:49, 24 April 2019
Line 1:		Line 1:

	==Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation==		==Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation==
-	<StructureSection load='4qqu' size='340' side='right' caption='[[4qqu]], [[Resolution\|resolution]] 2.98Å' scene=''>	+	<StructureSection load='4qqu' size='340' side='right'caption='[[4qqu]], [[Resolution\|resolution]] 2.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4qqu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QQU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4qqu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11006_[[candida_stellatoidea]] Atcc 11006 [[candida stellatoidea]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QQU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=39S:N-[4-({[(6S)-2-AMINO-4-OXO-3,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GAMMA-GLUTAMYL-L-GAMMA-GLUTAMYL-L-GLUTAMIC+ACID'>39S</scene>, <scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=39S:N-[4-({[(6S)-2-AMINO-4-OXO-3,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GAMMA-GLUTAMYL-L-GAMMA-GLUTAMYL-L-GLUTAMIC+ACID'>39S</scene>, <scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CaO19.10083, CaO19.2551, MET6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5476 ATCC 11006 [[Candida stellatoidea]]])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqu OCA], [http://pdbe.org/4qqu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qqu RCSB], [http://www.ebi.ac.uk/pdbsum/4qqu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqu OCA], [http://pdbe.org/4qqu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qqu RCSB], [http://www.ebi.ac.uk/pdbsum/4qqu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqu ProSAT]</span></td></tr>
Line 24:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase]]		[[Category: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase]]
		+	[[Category: Large Structures]]
	[[Category: Robertus, J D]]		[[Category: Robertus, J D]]
	[[Category: Ubhi, D K]]		[[Category: Ubhi, D K]]

OCA at 17:06, 4 January 2017

2017-01-04T17:06:39Z

←Older revision		Revision as of 17:06, 4 January 2017
Line 1:		Line 1:
		+
	==Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation==		==Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation==
	<StructureSection load='4qqu' size='340' side='right' caption='[[4qqu]], [[Resolution\|resolution]] 2.98Å' scene=''>		<StructureSection load='4qqu' size='340' side='right' caption='[[4qqu]], [[Resolution\|resolution]] 2.98Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=39S:N-[4-({[(6S)-2-AMINO-4-OXO-3,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GAMMA-GLUTAMYL-L-GAMMA-GLUTAMYL-L-GLUTAMIC+ACID'>39S</scene>, <scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=39S:N-[4-({[(6S)-2-AMINO-4-OXO-3,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GAMMA-GLUTAMYL-L-GAMMA-GLUTAMYL-L-GLUTAMIC+ACID'>39S</scene>, <scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qqu RCSB], [http://www.ebi.ac.uk/pdbsum/4qqu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqu OCA], [http://pdbe.org/4qqu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qqu RCSB], [http://www.ebi.ac.uk/pdbsum/4qqu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4qqu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:34, 14 January 2015

2015-01-14T13:34:36Z

←Older revision		Revision as of 13:34, 14 January 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation==
		+	<StructureSection load='4qqu' size='340' side='right' caption='[[4qqu]], [[Resolution\|resolution]] 2.98Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4qqu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QQU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=39S:N-[4-({[(6S)-2-AMINO-4-OXO-3,4,5,6,7,8-HEXAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GAMMA-GLUTAMYL-L-GAMMA-GLUTAMYL-L-GLUTAMIC+ACID'>39S</scene>, <scene name='pdbligand=HCS:2-AMINO-4-MERCAPTO-BUTYRIC+ACID'>HCS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4qqu RCSB], [http://www.ebi.ac.uk/pdbsum/4qqu PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/METE_CANAL METE_CANAL]] Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.[HAMAP-Rule:MF_00172]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cobalamin-independent methionine synthase enzyme catalyzes a challenging reaction: the direct transfer of a methyl from 5-methyl-tetrahydrofolate-glutamate3 to the l-homocysteine thiol. The enzyme has a dual (betaalpha)8 TIM barrel structure that binds, activates and brings the reactants into reaction proximity by conformational movements. In the previously observed open structures, the substrates bind too far apart to react, but we have captured a ternary complex with both substrates bound in a closed form of the enzyme. The closing is described in terms of a hinge between the N- and C-terminal TIM barrels and a rearrangement of key loops within the C domain. The substrate specificity can now be rationalized and the structure reveals His707 as the acid that protonates the THF leaving group through a water molecule trapped in the closed active site. The substrates are correctly oriented for an in-line attack by l-homocysteine on the N5-methyl.

-	The ~~entry 4qqu is ON HOLD until Paper Publication~~	+	The Cobalamin-Independent Methionine Synthase Enzyme Captured in a Substrate-Induced Closed Conformation.,Ubhi DK, Robertus JD J Mol Biol. 2014 Dec 27. pii: S0022-2836(14)00649-4. doi:, 10.1016/j.jmb.2014.12.014. PMID:25545590<ref>PMID:25545590</ref>

-	~~Authors: Ubhi~~, D.K.~~, Robertus, J.D~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of the cobalamin~~-~~independent methionine synthase enzyme in a closed conformation~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: Ubhi, D.K]]	+	__TOC__
-	[[Category: ~~Robertus, J.D~~]]	+	</StructureSection>
		+	[[Category: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase]]
		+	[[Category: Robertus, J D]]
		+	[[Category: Ubhi, D K]]
		+	[[Category: Closed conformation]]
		+	[[Category: Cobalamin-independent]]
		+	[[Category: Dual tim barrel]]
		+	[[Category: Fungal]]
		+	[[Category: Methionine synthase]]
		+	[[Category: Surface entropy reduction]]
		+	[[Category: Transferase]]

OCA at 11:31, 31 December 2014

2014-12-31T11:31:03Z

←Older revision		Revision as of 11:31, 31 December 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4qqu is ON HOLD	+	The entry 4qqu is ON HOLD until Paper Publication

	Authors: Ubhi, D.K., Robertus, J.D.		Authors: Ubhi, D.K., Robertus, J.D.

OCA at 16:02, 24 December 2014

2014-12-24T16:02:54Z

←Older revision		Revision as of 16:02, 24 December 2014
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	Description: Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation		Description: Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation
		+	[[Category: Unreleased Structures]]
		+	[[Category: Ubhi, D.K]]
		+	[[Category: Robertus, J.D]]

OCA at 06:38, 26 November 2014

2014-11-26T06:38:18Z

←Older revision		Revision as of 06:38, 26 November 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4qqu is ON HOLD ~~until Paper Publication~~	+	The entry 4qqu is ON HOLD

	Authors: Ubhi, D.K., Robertus, J.D.		Authors: Ubhi, D.K., Robertus, J.D.

	Description: Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation		Description: Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation

OCA at 08:14, 24 September 2014

2014-09-24T08:14:25Z

←Older revision		Revision as of 08:14, 24 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4qqu is ON HOLD	+	The entry 4qqu is ON HOLD until Paper Publication

	Authors: Ubhi, D.K., Robertus, J.D.		Authors: Ubhi, D.K., Robertus, J.D.

	Description: Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation		Description: Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation