4r7v - Revision history

OCA at 12:50, 1 March 2024

2024-03-01T12:50:51Z

←Older revision		Revision as of 12:50, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4r7v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R7V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4r7v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R7V FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7v OCA], [https://pdbe.org/4r7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r7v RCSB], [https://www.ebi.ac.uk/pdbsum/4r7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7v ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.73Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7v OCA], [https://pdbe.org/4r7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r7v RCSB], [https://www.ebi.ac.uk/pdbsum/4r7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ARRD3_HUMAN ARRD3_HUMAN]		[https://www.uniprot.org/uniprot/ARRD3_HUMAN ARRD3_HUMAN]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	ARRDC3 is one of six known human alpha-arrestins, and has been implicated in the downregulation of the beta2-adrenergic receptor (beta2AR). ARRDC3 consists of a two-lobed arrestin fold and a C-terminal tail containing two PPYX motifs. In the current model for receptor downregulation by ARRDC3, the arrestin fold portion is thought to bind the receptor, while the PPXY motifs recruit ubiquitin ligases of the NEDD4 family. Here we report the crystal structures of the N-terminal lobe of human ARRDC3 in two conformations, at 1.73 and 2.8 A resolution, respectively. The structures reveal a large electropositive region that is capable of binding phosphate ions of crystallization. Residues within the basic patch were shown to be important for binding to beta2AR, similar to the situation with beta-arrestins. This highlights potential parallels in receptor recognition between alpha- and beta-arrestins.
-
-	Insights into beta2-adrenergic receptor binding from structures of the N-terminal lobe of ARRDC3.,Qi S, O'Hayre M, Gutkind JS, Hurley JH Protein Sci. 2014 Sep 12. doi: 10.1002/pro.2549. PMID:25220262<ref>PMID:25220262</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4r7v" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:43, 2 March 2023

2023-03-02T06:43:42Z

←Older revision		Revision as of 06:43, 2 March 2023
Line 3:		Line 3:
	<StructureSection load='4r7v' size='340' side='right'caption='[[4r7v]], [[Resolution\|resolution]] 1.73Å' scene=''>		<StructureSection load='4r7v' size='340' side='right'caption='[[4r7v]], [[Resolution\|resolution]] 1.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4r7v]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4R7V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4r7v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R7V FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7x\|4r7x]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7v OCA], [https://pdbe.org/4r7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r7v RCSB], [https://www.ebi.ac.uk/pdbsum/4r7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7v ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARRDC3, KIAA1376 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4r7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7v OCA], [~~http~~://pdbe.org/4r7v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4r7v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4r7v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7v ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ARRD3_HUMAN ARRD3_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hurley, J]]	+	[[Category: Hurley J]]
-	[[Category: Qi, S]]	+	[[Category: Qi S]]
-	~~[[Category: Arrestin fold]]~~	+
-	~~[[Category: Beta 2 adrenergic receptor]]~~	+
-	~~[[Category: Gpcr down regulation]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 10:13, 1 May 2019

2019-05-01T10:13:15Z

←Older revision		Revision as of 10:13, 1 May 2019
Line 1:		Line 1:

	==Crystal structure of N-lobe of human ARRDC3(1-165)==		==Crystal structure of N-lobe of human ARRDC3(1-165)==
-	<StructureSection load='4r7v' size='340' side='right' caption='[[4r7v]], [[Resolution\|resolution]] 1.73Å' scene=''>	+	<StructureSection load='4r7v' size='340' side='right'caption='[[4r7v]], [[Resolution\|resolution]] 1.73Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4r7v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R7V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4r7v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R7V FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7x\|4r7x]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7x\|4r7x]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARRDC3, KIAA1376 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7v OCA], [http://pdbe.org/4r7v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r7v RCSB], [http://www.ebi.ac.uk/pdbsum/4r7v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7v OCA], [http://pdbe.org/4r7v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r7v RCSB], [http://www.ebi.ac.uk/pdbsum/4r7v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7v ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Hurley, J]]		[[Category: Hurley, J]]
	[[Category: Qi, S]]		[[Category: Qi, S]]

OCA at 17:18, 18 January 2017

2017-01-18T17:18:47Z

←Older revision		Revision as of 17:18, 18 January 2017
Line 1:		Line 1:
		+
	==Crystal structure of N-lobe of human ARRDC3(1-165)==		==Crystal structure of N-lobe of human ARRDC3(1-165)==
	<StructureSection load='4r7v' size='340' side='right' caption='[[4r7v]], [[Resolution\|resolution]] 1.73Å' scene=''>		<StructureSection load='4r7v' size='340' side='right' caption='[[4r7v]], [[Resolution\|resolution]] 1.73Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4r7v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R7V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4r7v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R7V FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7x\|4r7x]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7x\|4r7x]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r7v RCSB], [http://www.ebi.ac.uk/pdbsum/4r7v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7v OCA], [http://pdbe.org/4r7v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r7v RCSB], [http://www.ebi.ac.uk/pdbsum/4r7v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r7v ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4r7v" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:52, 10 December 2014

2014-12-10T11:52:25Z

←Older revision		Revision as of 11:52, 10 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4r7v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R7V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4r7v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R7V FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7x\|4r7x]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7x\|4r7x]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r7v RCSB], [http://www.ebi.ac.uk/pdbsum/4r7v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r7v RCSB], [http://www.ebi.ac.uk/pdbsum/4r7v PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Hurley, J.]]	+	[[Category: Hurley, J]]
-	[[Category: Qi, S.]]	+	[[Category: Qi, S]]
	[[Category: Arrestin fold]]		[[Category: Arrestin fold]]
	[[Category: Beta 2 adrenergic receptor]]		[[Category: Beta 2 adrenergic receptor]]
	[[Category: Gpcr down regulation]]		[[Category: Gpcr down regulation]]
	[[Category: Protein binding]]		[[Category: Protein binding]]

OCA at 00:12, 2 October 2014

2014-10-02T00:12:16Z

←Older revision		Revision as of 00:12, 2 October 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of N-lobe of human ARRDC3(1-165)==
		+	<StructureSection load='4r7v' size='340' side='right' caption='[[4r7v]], [[Resolution\|resolution]] 1.73Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4r7v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R7V FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4r7x\|4r7x]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r7v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r7v RCSB], [http://www.ebi.ac.uk/pdbsum/4r7v PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ARRDC3 is one of six known human alpha-arrestins, and has been implicated in the downregulation of the beta2-adrenergic receptor (beta2AR). ARRDC3 consists of a two-lobed arrestin fold and a C-terminal tail containing two PPYX motifs. In the current model for receptor downregulation by ARRDC3, the arrestin fold portion is thought to bind the receptor, while the PPXY motifs recruit ubiquitin ligases of the NEDD4 family. Here we report the crystal structures of the N-terminal lobe of human ARRDC3 in two conformations, at 1.73 and 2.8 A resolution, respectively. The structures reveal a large electropositive region that is capable of binding phosphate ions of crystallization. Residues within the basic patch were shown to be important for binding to beta2AR, similar to the situation with beta-arrestins. This highlights potential parallels in receptor recognition between alpha- and beta-arrestins.

-	~~The entry 4r7v is ON HOLD until Paper Publication~~	+	Insights into beta2-adrenergic receptor binding from structures of the N-terminal lobe of ARRDC3.,Qi S, O'Hayre M, Gutkind JS, Hurley JH Protein Sci. 2014 Sep 12. doi: 10.1002/pro.2549. PMID:25220262<ref>PMID:25220262</ref>

-	~~Authors: Qi~~, S., Hurley, J.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of N-lobe of human ARRDC3(1-165)~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hurley, J.]]
		+	[[Category: Qi, S.]]
		+	[[Category: Arrestin fold]]
		+	[[Category: Beta 2 adrenergic receptor]]
		+	[[Category: Gpcr down regulation]]
		+	[[Category: Protein binding]]

OCA at 08:24, 24 September 2014

2014-09-24T08:24:50Z

←Older revision		Revision as of 08:24, 24 September 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4r7v is ON HOLD	+	The entry 4r7v is ON HOLD until Paper Publication

	Authors: Qi, S., Hurley, J.		Authors: Qi, S., Hurley, J.

	Description: Crystal structure of N-lobe of human ARRDC3(1-165)		Description: Crystal structure of N-lobe of human ARRDC3(1-165)

OCA at 08:24, 10 September 2014

2014-09-10T08:24:52Z

←Older revision		Revision as of 08:24, 10 September 2014
Line 3:		Line 3:
	The entry 4r7v is ON HOLD		The entry 4r7v is ON HOLD

-	Authors: Qi, ~~Shiqian~~, Hurley, ~~James~~	+	Authors: Qi, S., Hurley, J.

	Description: Crystal structure of N-lobe of human ARRDC3(1-165)		Description: Crystal structure of N-lobe of human ARRDC3(1-165)

OCA: Protected "4r7v" [edit=sysop:move=sysop]

2014-09-04T04:19:51Z

Protected "4r7v" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:19, 4 September 2014

OCA: New page: '''Unreleased structure''' The entry 4r7v is ON HOLD Authors: Qi, Shiqian, Hurley, James Description: Crystal structure of N-lobe of human ARRDC3(1-165)

2014-09-04T04:19:49Z

New page: '''Unreleased structure''' The entry 4r7v is ON HOLD Authors: Qi, Shiqian, Hurley, James Description: Crystal structure of N-lobe of human ARRDC3(1-165)

New page

'''Unreleased structure'''

The entry 4r7v is ON HOLD

Authors: Qi, Shiqian, Hurley, James

Description: Crystal structure of N-lobe of human ARRDC3(1-165)