4rav - Revision history

OCA at 06:47, 2 March 2023

2023-03-02T06:47:56Z

←Older revision		Revision as of 06:47, 2 March 2023
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	<StructureSection load='4rav' size='340' side='right'caption='[[4rav]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4rav' size='340' side='right'caption='[[4rav]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rav]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RAV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4RAV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rav]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RAV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RAV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4rav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rav OCA], [~~http~~://pdbe.org/4rav PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4rav RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4rav PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4rav ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rav OCA], [https://pdbe.org/4rav PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rav RCSB], [https://www.ebi.ac.uk/pdbsum/4rav PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rav ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/HD_HUMAN HD_HUMAN]] Juvenile Huntington disease;Huntington disease. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/HD_HUMAN HD_HUMAN] Juvenile Huntington disease;Huntington disease. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HD_HUMAN HD_HUMAN]] May play a role in microtubule-mediated transport or vesicle function.	+	[https://www.uniprot.org/uniprot/HD_HUMAN HD_HUMAN] May play a role in microtubule-mediated transport or vesicle function.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chirgadze~~, D Y]]~~	+	[[Category: Chirgadze DY]]
-	~~[[Category: Dobson, C M~~]]	+	[[Category: De Genst E]]
-	[[Category: Genst, E De]]	+	[[Category: Dobson CM]]
-	[[Category: ~~1-17 residues of huntingtin]]~~	+
-	~~[[Category: Immune system-apoptosis complex]]~~	+
-	~~[[Category: Immunity]]~~	+
-	~~[[Category: Immunoglobulin fold~~]]	+

OCA at 10:25, 1 May 2019

2019-05-01T10:25:19Z

←Older revision		Revision as of 10:25, 1 May 2019
Line 1:		Line 1:

	==Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin==		==Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin==
-	<StructureSection load='4rav' size='340' side='right' caption='[[4rav]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4rav' size='340' side='right'caption='[[4rav]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rav]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RAV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rav]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RAV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rav OCA], [http://pdbe.org/4rav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rav RCSB], [http://www.ebi.ac.uk/pdbsum/4rav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rav ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rav OCA], [http://pdbe.org/4rav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rav RCSB], [http://www.ebi.ac.uk/pdbsum/4rav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rav ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Chirgadze, D Y]]		[[Category: Chirgadze, D Y]]
	[[Category: Dobson, C M]]		[[Category: Dobson, C M]]

OCA at 13:27, 4 May 2017

2017-05-04T13:27:27Z

←Older revision		Revision as of 13:27, 4 May 2017
Line 1:		Line 1:
		+
	==Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin==		==Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin==
	<StructureSection load='4rav' size='340' side='right' caption='[[4rav]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4rav' size='340' side='right' caption='[[4rav]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4rav]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RAV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4rav]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RAV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rav OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rav RCSB], [http://www.ebi.ac.uk/pdbsum/4rav PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rav OCA], [http://pdbe.org/4rav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rav RCSB], [http://www.ebi.ac.uk/pdbsum/4rav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rav ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4rav" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Huntingtin\|Huntingtin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:36, 29 July 2015

2015-07-29T14:36:19Z

←Older revision		Revision as of 14:36, 29 July 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin==
		+	<StructureSection load='4rav' size='340' side='right' caption='[[4rav]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4rav]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RAV FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rav OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rav RCSB], [http://www.ebi.ac.uk/pdbsum/4rav PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/HD_HUMAN HD_HUMAN]] Juvenile Huntington disease;Huntington disease. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HD_HUMAN HD_HUMAN]] May play a role in microtubule-mediated transport or vesicle function.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Huntington's disease is triggered by misfolding of fragments of mutant forms of the huntingtin protein (mHTT) with aberrant polyglutamine expansions. The C4 single-chain Fv antibody (scFv) binds to the first 17 residues of huntingtin [HTT(1-17)] and generates substantial protection against multiple phenotypic pathologies in situ and in vivo. We show in this paper that C4 scFv inhibits amyloid formation by exon1 fragments of huntingtin in vitro and elucidate the structural basis for this inhibition and protection by determining the crystal structure of the complex of C4 scFv and HTT(1-17). The peptide binds with residues 3-11 forming an amphipathic helix that makes contact with the antibody fragment in such a way that the hydrophobic face of this helix is shielded from the solvent. Residues 12-17 of the peptide are in an extended conformation and interact with the same region of another C4 scFv:HTT(1-17) complex in the asymmetric unit, resulting in a beta-sheet interface within a dimeric C4 scFv:HTT(1-17) complex. The nature of this scFv-peptide complex was further explored in solution by high-resolution NMR and physicochemical analysis of species in solution. The results provide insights into the manner in which C4 scFv inhibits the aggregation of HTT, and hence into its therapeutic potential, and suggests a structural basis for the initial interactions that underlie the formation of disease-associated amyloid fibrils by HTT.

-	~~The entry 4rav is ON HOLD until Paper Publication~~	+	Structure of a single-chain Fv bound to the 17 N-terminal residues of huntingtin provides insights into pathogenic amyloid formation and suppression.,De Genst E, Chirgadze DY, Klein FA, Butler DC, Matak-Vinkovic D, Trottier Y, Huston JS, Messer A, Dobson CM J Mol Biol. 2015 Jun 19;427(12):2166-78. doi: 10.1016/j.jmb.2015.03.021. Epub, 2015 Apr 8. PMID:25861763<ref>PMID:25861763</ref>

-	~~Authors: De Genst~~, E., Chirgadze, D.Y., Dobson, C.M.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Chirgadze, D.Y~~]]	+	__TOC__
-	[[Category: ~~De Genst, E~~]]	+	</StructureSection>
-	[[Category: ~~Dobson, C.M~~]]	+	[[Category: Chirgadze, D Y]]
		+	[[Category: Dobson, C M]]
		+	[[Category: Genst, E De]]
		+	[[Category: 1-17 residues of huntingtin]]
		+	[[Category: Immune system-apoptosis complex]]
		+	[[Category: Immunity]]
		+	[[Category: Immunoglobulin fold]]

OCA at 16:41, 24 December 2014

2014-12-24T16:41:49Z

←Older revision		Revision as of 16:41, 24 December 2014
Line 6:		Line 6:

	Description: Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin		Description: Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin
		+	[[Category: Unreleased Structures]]
		+	[[Category: Chirgadze, D.Y]]
		+	[[Category: De Genst, E]]
		+	[[Category: Dobson, C.M]]

OCA at 10:04, 29 October 2014

2014-10-29T10:04:03Z

←Older revision		Revision as of 10:04, 29 October 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4rav is ON HOLD	+	The entry 4rav is ON HOLD until Paper Publication

	Authors: De Genst, E., Chirgadze, D.Y., Dobson, C.M.		Authors: De Genst, E., Chirgadze, D.Y., Dobson, C.M.

	Description: Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin		Description: Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin

OCA at 23:07, 1 October 2014

2014-10-01T23:07:55Z

←Older revision		Revision as of 23:07, 1 October 2014
Line 3:		Line 3:
	The entry 4rav is ON HOLD		The entry 4rav is ON HOLD

-	Authors: De Genst, E, Chirgadze, D.Y., Dobson, C.M.	+	Authors: De Genst, E., Chirgadze, D.Y., Dobson, C.M.

	Description: Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin		Description: Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin

OCA: Protected "4rav" [edit=sysop:move=sysop]

2014-09-24T08:29:26Z

Protected "4rav" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:29, 24 September 2014

OCA: New page: '''Unreleased structure''' The entry 4rav is ON HOLD Authors: De Genst, E, Chirgadze, D.Y., Dobson, C.M. Description: Crystal structure of scFvC4 in complex with the first 17 AA of hun...

2014-09-24T08:29:24Z

New page: '''Unreleased structure''' The entry 4rav is ON HOLD Authors: De Genst, E, Chirgadze, D.Y., Dobson, C.M. Description: Crystal structure of scFvC4 in complex with the first 17 AA of hun...

New page

'''Unreleased structure'''

The entry 4rav is ON HOLD

Authors: De Genst, E, Chirgadze, D.Y., Dobson, C.M.

Description: Crystal structure of scFvC4 in complex with the first 17 AA of huntingtin