4rcv - Revision history

OCA at 17:48, 20 September 2023

2023-09-20T17:48:02Z

←Older revision		Revision as of 17:48, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4rcv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RCV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4rcv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RCV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DE2:1-DEOXY-L-ERYTHRULOSE'>DE2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PO3:PHOSPHITE+ION'>PO3</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.294Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DE2:1-DEOXY-L-ERYTHRULOSE'>DE2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PO3:PHOSPHITE+ION'>PO3</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rcv OCA], [https://pdbe.org/4rcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rcv RCSB], [https://www.ebi.ac.uk/pdbsum/4rcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rcv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rcv OCA], [https://pdbe.org/4rcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rcv RCSB], [https://www.ebi.ac.uk/pdbsum/4rcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rcv ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:50, 2 March 2023

2023-03-02T06:50:07Z

←Older revision		Revision as of 06:50, 2 March 2023
Line 3:		Line 3:
	<StructureSection load='4rcv' size='340' side='right'caption='[[4rcv]], [[Resolution\|resolution]] 2.29Å' scene=''>		<StructureSection load='4rcv' size='340' side='right'caption='[[4rcv]], [[Resolution\|resolution]] 2.29Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rcv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RCV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4RCV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rcv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RCV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DE2:1-DEOXY-L-ERYTHRULOSE'>DE2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PO3:PHOSPHITE+ION'>PO3</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DE2:1-DEOXY-L-ERYTHRULOSE'>DE2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PO3:PHOSPHITE+ION'>PO3</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ooe\|4ooe]], [[4oof\|4oof]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rcv OCA], [https://pdbe.org/4rcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rcv RCSB], [https://www.ebi.ac.uk/pdbsum/4rcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rcv ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dxr, P425_02990, RVBD_2870c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-deoxy-D-xylulose-5-phosphate_reductoisomerase 1-deoxy-D-xylulose-5-phosphate reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.267 1.1.1.267] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4rcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rcv OCA], [~~http~~://pdbe.org/4rcv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4rcv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4rcv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4rcv ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~I6YAH0_MYCTU I6YAH0_MYCTU]~~] Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) (By similarity).~~[HAMAP-Rule:MF_00183][SAAS:SAAS026877_004_013509]~~	+	[https://www.uniprot.org/uniprot/DXR_MYCTU DXR_MYCTU] Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 20:

	==See Also==		==See Also==
-	*[[DXP reductoisomerase\|DXP reductoisomerase]]	+	*[[DXP reductoisomerase 3D Structures\|DXP reductoisomerase 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 1-deoxy-D-xylulose-5-phosphate reductoisomerase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Allen~~, C L~~]]	+	[[Category: Allen CL]]
-	[[Category: Gulick~~, A M~~]]	+	[[Category: Gulick AM]]
-	[[Category: Kholodar~~, S A~~]]	+	[[Category: Kholodar SA]]
-	[[Category: Murkin~~, A S]]~~	+	[[Category: Murkin AS]]
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Reductoisomerase~~]]	+

OCA at 09:56, 1 May 2019

2019-05-01T09:56:57Z

←Older revision		Revision as of 09:56, 1 May 2019
Line 1:		Line 1:

	==M. tuberculosis 1-deoxy-d-xylulose-5-phosphate reductoisomerase bound to 1-deoxy-L-erythrulose==		==M. tuberculosis 1-deoxy-d-xylulose-5-phosphate reductoisomerase bound to 1-deoxy-L-erythrulose==
-	<StructureSection load='4rcv' size='340' side='right' caption='[[4rcv]], [[Resolution\|resolution]] 2.29Å' scene=''>	+	<StructureSection load='4rcv' size='340' side='right'caption='[[4rcv]], [[Resolution\|resolution]] 2.29Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rcv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RCV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rcv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RCV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DE2:1-DEOXY-L-ERYTHRULOSE'>DE2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PO3:PHOSPHITE+ION'>PO3</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DE2:1-DEOXY-L-ERYTHRULOSE'>DE2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PO3:PHOSPHITE+ION'>PO3</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ooe\|4ooe]], [[4oof\|4oof]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ooe\|4ooe]], [[4oof\|4oof]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dxr, P425_02990, RVBD_2870c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-deoxy-D-xylulose-5-phosphate_reductoisomerase 1-deoxy-D-xylulose-5-phosphate reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.267 1.1.1.267] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-deoxy-D-xylulose-5-phosphate_reductoisomerase 1-deoxy-D-xylulose-5-phosphate reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.267 1.1.1.267] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rcv OCA], [http://pdbe.org/4rcv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rcv RCSB], [http://www.ebi.ac.uk/pdbsum/4rcv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rcv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rcv OCA], [http://pdbe.org/4rcv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rcv RCSB], [http://www.ebi.ac.uk/pdbsum/4rcv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rcv ProSAT]</span></td></tr>
Line 28:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: 1-deoxy-D-xylulose-5-phosphate reductoisomerase]]		[[Category: 1-deoxy-D-xylulose-5-phosphate reductoisomerase]]
		+	[[Category: Large Structures]]
		+	[[Category: Myctu]]
	[[Category: Allen, C L]]		[[Category: Allen, C L]]
	[[Category: Gulick, A M]]		[[Category: Gulick, A M]]

OCA at 17:28, 18 January 2017

2017-01-18T17:28:56Z

←Older revision		Revision as of 17:28, 18 January 2017
Line 1:		Line 1:
		+
	==M. tuberculosis 1-deoxy-d-xylulose-5-phosphate reductoisomerase bound to 1-deoxy-L-erythrulose==		==M. tuberculosis 1-deoxy-d-xylulose-5-phosphate reductoisomerase bound to 1-deoxy-L-erythrulose==
	<StructureSection load='4rcv' size='340' side='right' caption='[[4rcv]], [[Resolution\|resolution]] 2.29Å' scene=''>		<StructureSection load='4rcv' size='340' side='right' caption='[[4rcv]], [[Resolution\|resolution]] 2.29Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ooe\|4ooe]], [[4oof\|4oof]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ooe\|4ooe]], [[4oof\|4oof]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-deoxy-D-xylulose-5-phosphate_reductoisomerase 1-deoxy-D-xylulose-5-phosphate reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.267 1.1.1.267] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-deoxy-D-xylulose-5-phosphate_reductoisomerase 1-deoxy-D-xylulose-5-phosphate reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.267 1.1.1.267] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rcv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rcv RCSB], [http://www.ebi.ac.uk/pdbsum/4rcv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rcv OCA], [http://pdbe.org/4rcv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rcv RCSB], [http://www.ebi.ac.uk/pdbsum/4rcv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rcv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4rcv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DXP reductoisomerase\|DXP reductoisomerase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:01, 18 March 2015

2015-03-18T12:01:58Z

←Older revision		Revision as of 12:01, 18 March 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==M. tuberculosis 1-deoxy-d-xylulose-5-phosphate reductoisomerase bound to 1-deoxy-L-erythrulose==
		+	<StructureSection load='4rcv' size='340' side='right' caption='[[4rcv]], [[Resolution\|resolution]] 2.29Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4rcv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RCV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RCV FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DE2:1-DEOXY-L-ERYTHRULOSE'>DE2</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PO3:PHOSPHITE+ION'>PO3</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ooe\|4ooe]], [[4oof\|4oof]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1-deoxy-D-xylulose-5-phosphate_reductoisomerase 1-deoxy-D-xylulose-5-phosphate reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.267 1.1.1.267] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rcv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rcv RCSB], [http://www.ebi.ac.uk/pdbsum/4rcv PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/I6YAH0_MYCTU I6YAH0_MYCTU]] Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) (By similarity).[HAMAP-Rule:MF_00183][SAAS:SAAS026877_004_013509]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Several mechanistically unrelated enzymes utilize the binding energy of their substrate's nonreacting phosphoryl group to accelerate catalysis. Evidence for the involvement of the phosphodianion in transition state formation has come from reactions of the substrate in pieces, in which reaction of a truncated substrate lacking its phosphorylmethyl group is activated by inorganic phosphite. What has remained unknown until now is how the phosphodianion group influences the reaction energetics at different points along the reaction coordinate. 1-Deoxy-d-xylulose-5-phosphate (DXP) reductoisomerase (DXR), which catalyzes the isomerization of DXP to 2-C-methyl-d-erythrose 4-phosphate (MEsP) and subsequent NADPH-dependent reduction, presents a unique opportunity to address this concern. Previously, we have reported the effect of covalently linked phosphate on the energetics of DXP turnover. Through the use of chemically synthesized MEsP and its phosphate-truncated analogue, 2-C-methyl-d-glyceraldehyde, the current study revealed a loss of 6.1 kcal/mol of kinetic barrier stabilization upon truncation, of which 4.4 kcal/mol was regained in the presence of phosphite dianion. The activating effect of phosphite was accompanied by apparent tightening of its interactions within the active site at the intermediate stage of the reaction, suggesting a role of the phosphodianion in disfavoring intermediate release and in modulation of the on-enzyme isomerization equilibrium. The results of kinetic isotope effect and structural studies indicate rate limitation by physical steps when the covalent linkage is severed. These striking differences in the energetics of the natural reaction and the reactions in pieces provide a deeper insight into the contribution of enzyme-phosphodianion interactions to the reaction coordinate.

-	The ~~entry 4rcv is ON HOLD until Paper Publication~~	+	The role of phosphate in a multistep enzymatic reaction: reactions of the substrate and intermediate in pieces.,Kholodar SA, Allen CL, Gulick AM, Murkin AS J Am Chem Soc. 2015 Feb 25;137(7):2748-56. doi: 10.1021/ja512911f. Epub 2015 Feb , 10. PMID:25642788<ref>PMID:25642788</ref>

-	~~Authors: Gulick~~, A.~~M., Allen, C.L., Kholodar,~~ S.~~A., Murkin, A.S~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~M. tuberculosis~~ 1-deoxy-d-xylulose-5-phosphate reductoisomerase ~~bound to 1-deoxy-~~L~~-erythrulose~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Allen~~, ~~C.L~~]]	+	__TOC__
-	[[Category: Murkin, A.S]]	+	</StructureSection>
-	[[Category: ~~Gulick, A.M~~]]	+	[[Category: 1-deoxy-D-xylulose-5-phosphate reductoisomerase]]
-	[[Category: ~~Kholodar, S.A~~]]	+	[[Category: Allen, C L]]
		+	[[Category: Gulick, A M]]
		+	[[Category: Kholodar, S A]]
		+	[[Category: Murkin, A S]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Reductoisomerase]]

OCA at 16:43, 24 December 2014

2014-12-24T16:43:44Z

←Older revision		Revision as of 16:43, 24 December 2014
Line 6:		Line 6:

	Description: M. tuberculosis 1-deoxy-d-xylulose-5-phosphate reductoisomerase bound to 1-deoxy-L-erythrulose		Description: M. tuberculosis 1-deoxy-d-xylulose-5-phosphate reductoisomerase bound to 1-deoxy-L-erythrulose
		+	[[Category: Unreleased Structures]]
		+	[[Category: Allen, C.L]]
		+	[[Category: Murkin, A.S]]
		+	[[Category: Gulick, A.M]]
		+	[[Category: Kholodar, S.A]]

OCA at 23:09, 1 October 2014

2014-10-01T23:09:05Z

←Older revision		Revision as of 23:09, 1 October 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4rcv is ON HOLD	+	The entry 4rcv is ON HOLD until Paper Publication

-	Authors: Gulick, A. M., Allen, C. L., Kholodar, S. A., Murkin, A. S.	+	Authors: Gulick, A.M., Allen, C.L., Kholodar, S.A., Murkin, A.S.

	Description: M. tuberculosis 1-deoxy-d-xylulose-5-phosphate reductoisomerase bound to 1-deoxy-L-erythrulose		Description: M. tuberculosis 1-deoxy-d-xylulose-5-phosphate reductoisomerase bound to 1-deoxy-L-erythrulose

OCA: Protected "4rcv" [edit=sysop:move=sysop]

2014-09-24T08:33:49Z

Protected "4rcv" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:33, 24 September 2014

OCA: New page: '''Unreleased structure''' The entry 4rcv is ON HOLD Authors: Gulick, A. M., Allen, C. L., Kholodar, S. A., Murkin, A. S. Description: M. tuberculosis 1-deoxy-d-xylulose-5-phosphate re...

2014-09-24T08:33:47Z

New page: '''Unreleased structure''' The entry 4rcv is ON HOLD Authors: Gulick, A. M., Allen, C. L., Kholodar, S. A., Murkin, A. S. Description: M. tuberculosis 1-deoxy-d-xylulose-5-phosphate re...

New page

'''Unreleased structure'''

The entry 4rcv is ON HOLD

Authors: Gulick, A. M., Allen, C. L., Kholodar, S. A., Murkin, A. S.

Description: M. tuberculosis 1-deoxy-d-xylulose-5-phosphate reductoisomerase bound to 1-deoxy-L-erythrulose