4rnf - Revision history

OCA at 12:53, 1 March 2024

2024-03-01T12:53:56Z

←Older revision		Revision as of 12:53, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4rnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RNF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4rnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RNF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [https://pdbe.org/4rnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [https://www.ebi.ac.uk/pdbsum/4rnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rnf ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.85Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [https://pdbe.org/4rnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [https://www.ebi.ac.uk/pdbsum/4rnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rnf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q9HVI8_PSEAE Q9HVI8_PSEAE]		[https://www.uniprot.org/uniprot/Q9HVI8_PSEAE Q9HVI8_PSEAE]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Diguanylate cyclases (DGC) and phosphodiesterases (PDE), respectively synthesise and hydrolyse the secondary messenger cyclic dimeric GMP (c-di-GMP), and both activities are often found in a single protein. Intracellular c-di-GMP levels in turn regulate bacterial motility, virulence and biofilm formation. We report the first structure of a tandem DGC-PDE fragment, in which the catalytic domains are shown to be active. Two phosphodiesterase states are distinguished by active site formation. The structures, in the presence or absence of c-di-GMP, suggest that dimerisation and binding pocket formation are linked, with dimerisation being required for catalytic activity. An understanding of PDE activation is important, as biofilm dispersal via c-di-GMP hydrolysis has therapeutic effects on chronic infections.
-
-	Formation and dimerization of the phosphodiesterase active site of the Pseudomonas aeruginosa MorA, a bi-functional c-di-GMP regulator.,Phippen CW, Mikolajek H, Schlaefli HG, Keevil CW, Webb JS, Tews I FEBS Lett. 2014 Dec 20;588(24):4631-6. doi: 10.1016/j.febslet.2014.11.002. Epub, 2014 Nov 11. PMID:25447517<ref>PMID:25447517</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4rnf" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:21, 8 March 2023

2023-03-08T08:21:02Z

←Older revision		Revision as of 08:21, 8 March 2023
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	<StructureSection load='4rnf' size='340' side='right'caption='[[4rnf]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='4rnf' size='340' side='right'caption='[[4rnf]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rnf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4RNF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RNF FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rnh\|4rnh]], [[4rni\|4rni]], [[4rnj\|4rnj]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [https://pdbe.org/4rnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [https://www.ebi.ac.uk/pdbsum/4rnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rnf ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">morA, PA4601 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [~~http~~://pdbe.org/4rnf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4rnf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4rnf ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9HVI8_PSEAE Q9HVI8_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Phippen~~, C W~~]]	+	[[Category: Phippen CW]]
-	[[Category: Tews, I]]	+	[[Category: Tews I]]
-	~~[[Category: C-di-gmp]]~~	+
-	~~[[Category: Diguanylate cyclase]]~~	+
-	~~[[Category: Gtp]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phosphodiesterase]]~~	+
-	~~[[Category: Tandem ggdef and eal domain]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:07, 1 May 2019

2019-05-01T10:07:50Z

←Older revision		Revision as of 10:07, 1 May 2019
Line 1:		Line 1:

	==PaMorA tandem diguanylate cyclase - mutant phosphodiesterase, apo form==		==PaMorA tandem diguanylate cyclase - mutant phosphodiesterase, apo form==
-	<StructureSection load='4rnf' size='340' side='right' caption='[[4rnf]], [[Resolution\|resolution]] 2.85Å' scene=''>	+	<StructureSection load='4rnf' size='340' side='right'caption='[[4rnf]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4rnf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RNF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4rnf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RNF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rnh\|4rnh]], [[4rni\|4rni]], [[4rnj\|4rnj]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rnh\|4rnh]], [[4rni\|4rni]], [[4rnj\|4rnj]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">morA, PA4601 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [http://pdbe.org/4rnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [http://www.ebi.ac.uk/pdbsum/4rnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rnf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [http://pdbe.org/4rnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [http://www.ebi.ac.uk/pdbsum/4rnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rnf ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseae]]
	[[Category: Phippen, C W]]		[[Category: Phippen, C W]]
	[[Category: Tews, I]]		[[Category: Tews, I]]

OCA at 18:53, 1 February 2017

2017-02-01T18:53:10Z

←Older revision		Revision as of 18:53, 1 February 2017
Line 1:		Line 1:
		+
	==PaMorA tandem diguanylate cyclase - mutant phosphodiesterase, apo form==		==PaMorA tandem diguanylate cyclase - mutant phosphodiesterase, apo form==
	<StructureSection load='4rnf' size='340' side='right' caption='[[4rnf]], [[Resolution\|resolution]] 2.85Å' scene=''>		<StructureSection load='4rnf' size='340' side='right' caption='[[4rnf]], [[Resolution\|resolution]] 2.85Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4rnf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RNF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4rnf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RNF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rnh\|4rnh]], [[4rni\|4rni]], [[4rnj\|4rnj]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rnh\|4rnh]], [[4rni\|4rni]], [[4rnj\|4rnj]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [http://www.ebi.ac.uk/pdbsum/4rnf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [http://pdbe.org/4rnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [http://www.ebi.ac.uk/pdbsum/4rnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rnf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4rnf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:29, 22 April 2015

2015-04-22T07:29:13Z

←Older revision		Revision as of 07:29, 22 April 2015
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [http://www.ebi.ac.uk/pdbsum/4rnf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [http://www.ebi.ac.uk/pdbsum/4rnf PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Diguanylate cyclases (DGC) and phosphodiesterases (PDE), respectively synthesise and hydrolyse the secondary messenger cyclic dimeric GMP (c-di-GMP), and both activities are often found in a single protein. Intracellular c-di-GMP levels in turn regulate bacterial motility, virulence and biofilm formation. We report the first structure of a tandem DGC-PDE fragment, in which the catalytic domains are shown to be active. Two phosphodiesterase states are distinguished by active site formation. The structures, in the presence or absence of c-di-GMP, suggest that dimerisation and binding pocket formation are linked, with dimerisation being required for catalytic activity. An understanding of PDE activation is important, as biofilm dispersal via c-di-GMP hydrolysis has therapeutic effects on chronic infections.
		+
		+	Formation and dimerization of the phosphodiesterase active site of the Pseudomonas aeruginosa MorA, a bi-functional c-di-GMP regulator.,Phippen CW, Mikolajek H, Schlaefli HG, Keevil CW, Webb JS, Tews I FEBS Lett. 2014 Dec 20;588(24):4631-6. doi: 10.1016/j.febslet.2014.11.002. Epub, 2014 Nov 11. PMID:25447517<ref>PMID:25447517</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:57, 19 November 2014

2014-11-19T12:57:08Z

←Older revision		Revision as of 12:57, 19 November 2014
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==PaMorA tandem diguanylate cyclase - mutant phosphodiesterase, apo form==
-		+	<StructureSection load='4rnf' size='340' side='right' caption='[[4rnf]], [[Resolution\|resolution]] 2.85Å' scene=''>
-	~~The entry~~ 4rnf is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4rnf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RNF FirstGlance]. <br>
-	~~Authors~~: ~~Phippen~~, C.W., Tews, I.	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4rnh\|4rnh]], [[4rni\|4rni]], [[4rnj\|4rnj]]</td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [http://www.ebi.ac.uk/pdbsum/4rnf PDBsum]</span></td></tr>
-	~~Description~~: ~~PaMorA tandem diguanylate~~ cyclase ~~-mutant phosphodiesterase, apo form~~	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Phippen, C W]]
		+	[[Category: Tews, I]]
		+	[[Category: C-di-gmp]]
		+	[[Category: Diguanylate cyclase]]
		+	[[Category: Gtp]]
		+	[[Category: Hydrolase]]
		+	[[Category: Phosphodiesterase]]
		+	[[Category: Tandem ggdef and eal domain]]
		+	[[Category: Transferase]]

OCA: Protected "4rnf" [edit=sysop:move=sysop]

2014-11-05T07:40:23Z

Protected "4rnf" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:40, 5 November 2014

OCA: New page: '''Unreleased structure''' The entry 4rnf is ON HOLD Authors: Phippen, C.W., Tews, I. Description: PaMorA tandem diguanylate cyclase -mutant phosphodiesterase, apo form

2014-11-05T07:40:22Z

New page: '''Unreleased structure''' The entry 4rnf is ON HOLD Authors: Phippen, C.W., Tews, I. Description: PaMorA tandem diguanylate cyclase -mutant phosphodiesterase, apo form

New page

'''Unreleased structure'''

The entry 4rnf is ON HOLD

Authors: Phippen, C.W., Tews, I.

Description: PaMorA tandem diguanylate cyclase -mutant phosphodiesterase, apo form