4sod - Revision history

OCA at 10:43, 15 March 2023

2023-03-15T10:43:16Z

←Older revision		Revision as of 10:43, 15 March 2023
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	<StructureSection load='4sod' size='340' side='right'caption='[[4sod]]' scene=''>		<StructureSection load='4sod' size='340' side='right'caption='[[4sod]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4SOD FirstGlance]. <br>	+	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4SOD FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4sod FirstGlance], [~~http~~://www.ebi.ac.uk/pdbsum/4sod PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4sod ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4sod FirstGlance], [https://www.ebi.ac.uk/pdbsum/4sod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4sod ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:45, 1 May 2019

2019-05-01T09:45:38Z

←Older revision		Revision as of 09:45, 1 May 2019
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	==RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE==		==RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE==
-	<StructureSection load='4sod' size='340' side='right' caption='[[4sod]]' scene=''>	+	<StructureSection load='4sod' size='340' side='right'caption='[[4sod]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4SOD FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4SOD FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sod FirstGlance], [http://www.ebi.ac.uk/pdbsum/4sod PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sod FirstGlance], [http://www.ebi.ac.uk/pdbsum/4sod PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4sod ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Theoretical Model]]		[[Category: Theoretical Model]]
		+	[[Category: Large Structures]]
	[[Category: Fisher, C L]]		[[Category: Fisher, C L]]
	[[Category: Kuhn, L A]]		[[Category: Kuhn, L A]]
	[[Category: Tainer, J A]]		[[Category: Tainer, J A]]

OCA at 11:09, 11 February 2016

2016-02-11T11:09:26Z

←Older revision		Revision as of 11:09, 11 February 2016
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		+	{{Theoretical_model}}
		+
	==RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE==		==RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE==
	<StructureSection load='4sod' size='340' side='right' caption='[[4sod]]' scene=''>		<StructureSection load='4sod' size='340' side='right' caption='[[4sod]]' scene=''>
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4sod" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Theoretical Model]]
	[[Category: Fisher, C L]]		[[Category: Fisher, C L]]
	[[Category: Kuhn, L A]]		[[Category: Kuhn, L A]]
	[[Category: Tainer, J A]]		[[Category: Tainer, J A]]

OCA at 15:11, 21 December 2014

2014-12-21T15:11:31Z

←Older revision		Revision as of 15:11, 21 December 2014
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-	~~{{Theoretical_model}}~~	+	==RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE==
		+	<StructureSection load='4sod' size='340' side='right' caption='[[4sod]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4SOD FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4sod FirstGlance], [http://www.ebi.ac.uk/pdbsum/4sod PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In order to improve the therapeutic effectiveness of human Cu,Zn superoxide dismutase (HSOD) by targeting it to cell surfaces and increasing its circulatory half-life, we have designed and expressed a heparin-binding derivative of HSOD. This design was based on the idea that structurally independent protein units, HSOD and the heparin-binding A+ helix from protein C inhibitor, could be combined with a carefully chosen linker, GlyProGly, to form a stable, bifunctional protein. The chimeric HSOD-GlyProGly-A+ protein was expressed and secreted to the periplasm of E. coli and had normal SOD activity. HSOD-GlyProGly-A+ had a significantly increased retention time relative to wild-type HSOD on a heparin affinity column, indicating that it was successfully targeted to heparin, and this binding was maintained at physiological ionic strength. When administered to mice, HSOD-GlyProGly-A+ had a half-life of approximately 15 minutes, twice that of wild-type HSOD. Our rational design approach should be generally applicable to the creation of bifunctional chimeric molecules.

-	~~{{STRUCTURE_4sod\| PDB=4sod \| SCENE= }}~~	+	Rational design and expression of a heparin-targeted human superoxide dismutase.,Boissinot M, Kuhn LA, Lee P, Fisher CL, Wang Y, Hallewell RA, Tainer JA Biochem Biophys Res Commun. 1993 Jan 15;190(1):250-6. PMID:8422249<ref>PMID:8422249</ref>
-	~~===RATIONAL DESIGN AND EXPRESSION OF A HEPARIN~~-~~TARGETED HUMAN SUPEROXIDE DISMUTASE===~~	+
-	~~{{ABSTRACT_PUBMED_8422249}}~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:008422249</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Fisher, C L]]		[[Category: Fisher, C L]]
	[[Category: Kuhn, L A]]		[[Category: Kuhn, L A]]
	[[Category: Tainer, J A]]		[[Category: Tainer, J A]]

OCA at 23:56, 24 March 2013

2013-03-24T23:56:46Z

←Older revision		Revision as of 23:56, 24 March 2013
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	{{Theoretical_model}}		{{Theoretical_model}}
-
-	[[Image:4sod.png\|left\|200px]]

	{{STRUCTURE_4sod\| PDB=4sod \| SCENE= }}		{{STRUCTURE_4sod\| PDB=4sod \| SCENE= }}
-
	===RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE===		===RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE===
-
	{{ABSTRACT_PUBMED_8422249}}		{{ABSTRACT_PUBMED_8422249}}

	==Reference==		==Reference==
-	<ref group="xtra">PMID:008422249</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008422249</ref><references group="xtra"/><references/>
	[[Category: Fisher, C L]]		[[Category: Fisher, C L]]
	[[Category: Kuhn, L A]]		[[Category: Kuhn, L A]]
	[[Category: Tainer, J A]]		[[Category: Tainer, J A]]

OCA: Protected "4sod" [edit=sysop:move=sysop]

2012-10-21T09:31:37Z

Protected "4sod" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:31, 21 October 2012

OCA at 09:31, 21 October 2012

2012-10-21T09:31:35Z

←Older revision		Revision as of 09:31, 21 October 2012
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	{{Theoretical_model}}		{{Theoretical_model}}
-	~~{{Seed}}~~	+
	[[Image:4sod.png\|left\|200px]]		[[Image:4sod.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_4sod\| PDB=4sod \| SCENE= }}		{{STRUCTURE_4sod\| PDB=4sod \| SCENE= }}

	===RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE===		===RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_8422249}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 8422249 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_8422249}}		{{ABSTRACT_PUBMED_8422249}}
-
-	==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SOD OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~8422249~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008422249</ref><references group="xtra"/>
	[[Category: Fisher, C L]]		[[Category: Fisher, C L]]
	[[Category: Kuhn, L A]]		[[Category: Kuhn, L A]]
	[[Category: Tainer, J A]]		[[Category: Tainer, J A]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 08:29:42 2010''

OCA at 05:29, 8 April 2010

2010-04-08T05:29:43Z

←Older revision		Revision as of 05:29, 8 April 2010
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-	~~'''Theoretical Model'''~~	+	{{Theoretical_model}}
		+	{{Seed}}
		+	[[Image:4sod.png\|left\|200px]]

-	The ~~entry 4SOD~~ is ~~a Theoretical Model titled 'RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE'~~.	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4sod", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4sod\| PDB=4sod \| SCENE= }}

-	~~[[Category:Theoretical Model]]~~	+	===RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE===


-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 30 09~~:58:~~55 2008~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_8422249}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 8422249 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_8422249}}
		+
		+	==About this Structure==
		+	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4SOD OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:8422249</ref><references group="xtra"/>
		+	[[Category: Fisher, C L]]
		+	[[Category: Kuhn, L A]]
		+	[[Category: Tainer, J A]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 08:29:42 2010''

OCA at 06:58, 30 July 2008

2008-07-30T06:58:56Z

←Older revision		Revision as of 06:58, 30 July 2008
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-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul ~~23 13~~:33:51 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 30 09:58:55 2008''

OCA: New page: '''Theoretical Model''' The entry 4SOD is a Theoretical Model titled 'RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE'. Category:Theoretical Model '...

2008-07-23T10:33:51Z

New page: '''Theoretical Model''' The entry 4SOD is a Theoretical Model titled 'RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE'. Category:Theoretical Model '...

New page

'''Theoretical Model'''

The entry 4SOD is a Theoretical Model titled 'RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE'.

[[Category:Theoretical Model]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 13:33:51 2008''