4tnv - Revision history

OCA at 07:20, 27 September 2023

2023-09-27T07:20:43Z

←Older revision		Revision as of 07:20, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tnv]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TNV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tnv]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TNV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [https://pdbe.org/4tnv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [https://www.ebi.ac.uk/pdbsum/4tnv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [https://pdbe.org/4tnv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [https://www.ebi.ac.uk/pdbsum/4tnv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnv ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:47, 15 March 2023

2023-03-15T10:47:34Z

←Older revision		Revision as of 10:47, 15 March 2023
Line 3:		Line 3:
	<StructureSection load='4tnv' size='340' side='right'caption='[[4tnv]], [[Resolution\|resolution]] 3.60Å' scene=''>		<StructureSection load='4tnv' size='340' side='right'caption='[[4tnv]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tnv]] is a 30 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Caeel Caeel~~] and [~~http~~://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4TNV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tnv]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TNV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnw\|4tnw]], [[3rhw\|3rhw]], [[3ri5\|3ri5]], [[3ria\|3ria]], [[3rif\|3rif]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [https://pdbe.org/4tnv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [https://www.ebi.ac.uk/pdbsum/4tnv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnv ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glc-1, CELE_F11A5.10, F11A5.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [~~http~~://pdbe.org/4tnv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4tnv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnv ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GLUCL_CAEEL GLUCL_CAEEL] Glutamate-gated chloride channel subunit; channel properties depend on the subunit composition. Glutamate binding triggers a rapidly reversible current in heteromeric channels formed by glc-1 and glc-2, while the anti-helmintic drug ivermectin and other avermectins trigger a permanently open channel configuration. Channels containing only glc-1 are activated by ivermectin, but not by glutamate alone (in vitro). The heteromeric channel formed by glc-1 and glc-2 is also activated by ibotenate, and it is blocked by picrotoxin and flufenamic acid (PubMed:7935817). Plays a role in the regulation of locomotor behavior (PubMed:16527366).<ref>PMID:16527366</ref> <ref>PMID:21572436</ref> <ref>PMID:7935817</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Caeel~~]]	+	[[Category: Caenorhabditis elegans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Althoff, T]]	+	[[Category: Althoff T]]
-	[[Category: Banerjee, S]]	+	[[Category: Banerjee S]]
-	[[Category: Gouaux, E]]	+	[[Category: Gouaux E]]
-	[[Category: Hibbs~~, R E]]~~	+	[[Category: Hibbs RE]]
-	~~[[Category: Cys-loop receptor]]~~	+
-	~~[[Category: Ligand-gated ion channel]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Neurotransmitter receptor]]~~	+
-	~~[[Category: Transport protein-immune system complex~~]]	+

OCA at 08:24, 18 December 2019

2019-12-18T08:24:07Z

←Older revision		Revision as of 08:24, 18 December 2019
Line 1:		Line 1:
-	~~{{Large structure}}~~	+
	==C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab in a non-conducting conformation==		==C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab in a non-conducting conformation==
-	<StructureSection load='4tnv' size='340' side='right' caption='[[4tnv]], [[Resolution\|resolution]] 3.60Å' scene=''>	+	<StructureSection load='4tnv' size='340' side='right'caption='[[4tnv]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tnv]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tnv]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNV FirstGlance]. <br>
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [http://pdbe.org/4tnv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [http://www.ebi.ac.uk/pdbsum/4tnv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [http://pdbe.org/4tnv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [http://www.ebi.ac.uk/pdbsum/4tnv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnv ProSAT]</span></td></tr>
	</table>		</table>
-	{{Large structure}}
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Caeel]]		[[Category: Caeel]]
		+	[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Althoff, T]]		[[Category: Althoff, T]]

OCA at 12:04, 6 November 2017

2017-11-06T12:04:06Z

←Older revision		Revision as of 12:04, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='4tnv' size='340' side='right' caption='[[4tnv]], [[Resolution\|resolution]] 3.60Å' scene=''>		<StructureSection load='4tnv' size='340' side='right' caption='[[4tnv]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tnv]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tnv]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnw\|4tnw]], [[3rhw\|3rhw]], [[3ri5\|3ri5]], [[3ria\|3ria]], [[3rif\|3rif]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnw\|4tnw]], [[3rhw\|3rhw]], [[3ri5\|3ri5]], [[3ria\|3ria]], [[3rif\|3rif]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">glc-1, CELE_F11A5.10, F11A5.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [http://pdbe.org/4tnv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [http://www.ebi.ac.uk/pdbsum/4tnv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [http://pdbe.org/4tnv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [http://www.ebi.ac.uk/pdbsum/4tnv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnv ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Caeel]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Althoff, T]]		[[Category: Althoff, T]]

OCA at 10:25, 11 August 2016

2016-08-11T10:25:40Z

←Older revision		Revision as of 10:25, 11 August 2016
Line 1:		Line 1:
		+	{{Large structure}}
	==C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab in a non-conducting conformation==		==C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab in a non-conducting conformation==
	<StructureSection load='4tnv' size='340' side='right' caption='[[4tnv]], [[Resolution\|resolution]] 3.60Å' scene=''>		<StructureSection load='4tnv' size='340' side='right' caption='[[4tnv]], [[Resolution\|resolution]] 3.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnw\|4tnw]], [[3rhw\|3rhw]], [[3ri5\|3ri5]], [[3ria\|3ria]], [[3rif\|3rif]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnw\|4tnw]], [[3rhw\|3rhw]], [[3ri5\|3ri5]], [[3ria\|3ria]], [[3rif\|3rif]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [http://www.ebi.ac.uk/pdbsum/4tnv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [http://pdbe.org/4tnv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [http://www.ebi.ac.uk/pdbsum/4tnv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tnv ProSAT]</span></td></tr>
	</table>		</table>
		+	{{Large structure}}
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4tnv" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:47, 5 January 2015

2015-01-05T14:47:25Z

←Older revision		Revision as of 14:47, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tnv]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tnv]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnw\|4tnw]], [[3rhw\|3rhw]], [[3ri5\|3ri5]], [[3ria\|3ria]], [[3rif\|3rif]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnw\|4tnw]], [[3rhw\|3rhw]], [[3ri5\|3ri5]], [[3ria\|3ria]], [[3rif\|3rif]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [http://www.ebi.ac.uk/pdbsum/4tnv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [http://www.ebi.ac.uk/pdbsum/4tnv PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Althoff, T.]]	+	[[Category: Althoff, T]]
-	[[Category: Banerjee, S.]]	+	[[Category: Banerjee, S]]
-	[[Category: Gouaux, E.]]	+	[[Category: Gouaux, E]]
-	[[Category: Hibbs, R E.]]	+	[[Category: Hibbs, R E]]
	[[Category: Cys-loop receptor]]		[[Category: Cys-loop receptor]]
	[[Category: Ligand-gated ion channel]]		[[Category: Ligand-gated ion channel]]

OCA at 03:20, 4 September 2014

2014-09-04T03:20:06Z

←Older revision		Revision as of 03:20, 4 September 2014
Line 9:		Line 9:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	~~Fast inhibitory neurotransmission is~~ essential ~~for nervous system function~~ and ~~is mediated by binding~~ of ~~inhibitory neurotransmitters to receptors~~ of ~~the~~ Cys-loop ~~family embedded in the membranes~~ of ~~neurons. Neurotransmitter binding triggers a conformational change in~~ the receptor, ~~opening an intrinsic chloride channel~~ and ~~thereby dampening neuronal excitability. Here we present the first three~~-~~dimensional structure, to~~ our ~~knowledge,~~ of ~~an inhibitory anion-selective Cys-loop receptor,~~ the ~~homopentameric~~ Caenorhabditis elegans glutamate-gated chloride channel ~~alpha~~ (GluCl)~~, at 3.3 A resolution. The X-ray structure of the GluCl-Fab~~ complex ~~was determined~~ with the allosteric agonist ivermectin ~~and in additional structures with~~ the ~~endogenous neurotransmitter l~~-~~glutamate~~ and the open~~-channel blocker picrotoxin~~. ~~Ivermectin~~, ~~used to treat river blindness, binds in~~ the ~~transmembrane domain~~ of the ~~receptor~~ and ~~stabilizes an~~ open~~-pore conformation. Glutamate binds in~~ the ~~classical agonist site at subunit interfaces~~, and ~~picrotoxin directly occludes~~ the ~~pore near its cytosolic base~~. GluCl ~~provides a framework for understanding mechanisms~~ of ~~fast inhibitory neurotransmission~~ and ~~allosteric~~ modulation of ~~Cys-loop receptors~~.	+	Cys-loop receptors are neurotransmitter-gated ion channels that are essential mediators of fast chemical neurotransmission and are associated with a large number of neurological diseases and disorders, as well as parasitic infections. Members of this ion channel superfamily mediate excitatory or inhibitory neurotransmission depending on their ligand and ion selectivity. Structural information for Cys-loop receptors comes from several sources including electron microscopic studies of the nicotinic acetylcholine receptor, high-resolution X-ray structures of extracellular domains and X-ray structures of bacterial orthologues. In 2011 our group published structures of the Caenorhabditis elegans glutamate-gated chloride channel (GluCl) in complex with the allosteric partial agonist ivermectin, which provided insights into the structure of a possibly open state of a eukaryotic Cys-loop receptor, the basis for anion selectivity and channel block, and the mechanism by which ivermectin and related molecules stabilize the open state and potentiate neurotransmitter binding. However, there remain unanswered questions about the mechanism of channel opening and closing, the location and nature of the shut ion channel gate, the transitions between the closed/resting, open/activated and closed/desensitized states, and the mechanism by which conformational changes are coupled between the extracellular, orthosteric agonist binding domain and the transmembrane, ion channel domain. Here we present two conformationally distinct structures of C. elegans GluCl in the absence of ivermectin. Structural comparisons reveal a quaternary activation mechanism arising from rigid-body movements between the extracellular and transmembrane domains and a mechanism for modulation of the receptor by phospholipids.

-	~~Principles~~ of ~~activation and permeation~~ in ~~an anion-selective~~ Cys-loop ~~receptor~~.,Hibbs RE, Gouaux E Nature. ~~2011 May 15~~. PMID:~~21572436~~<ref>PMID:~~21572436~~</ref>	+	X-ray structures of GluCl in apo states reveal a gating mechanism of Cys-loop receptors.,Althoff T, Hibbs RE, Banerjee S, Gouaux E Nature. 2014 Aug 21;512(7514):333-7. doi: 10.1038/nature13669. PMID:25143115<ref>PMID:25143115</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 09:07, 13 August 2014

2014-08-13T09:07:10Z

←Older revision		Revision as of 09:07, 13 August 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab in a non-conducting conformation==
		+	<StructureSection load='4tnv' size='340' side='right' caption='[[4tnv]], [[Resolution\|resolution]] 3.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4tnv]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TNV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TNV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4tnw\|4tnw]], [[3rhw\|3rhw]], [[3ri5\|3ri5]], [[3ria\|3ria]], [[3rif\|3rif]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tnv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tnv RCSB], [http://www.ebi.ac.uk/pdbsum/4tnv PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fast inhibitory neurotransmission is essential for nervous system function and is mediated by binding of inhibitory neurotransmitters to receptors of the Cys-loop family embedded in the membranes of neurons. Neurotransmitter binding triggers a conformational change in the receptor, opening an intrinsic chloride channel and thereby dampening neuronal excitability. Here we present the first three-dimensional structure, to our knowledge, of an inhibitory anion-selective Cys-loop receptor, the homopentameric Caenorhabditis elegans glutamate-gated chloride channel alpha (GluCl), at 3.3 A resolution. The X-ray structure of the GluCl-Fab complex was determined with the allosteric agonist ivermectin and in additional structures with the endogenous neurotransmitter l-glutamate and the open-channel blocker picrotoxin. Ivermectin, used to treat river blindness, binds in the transmembrane domain of the receptor and stabilizes an open-pore conformation. Glutamate binds in the classical agonist site at subunit interfaces, and picrotoxin directly occludes the pore near its cytosolic base. GluCl provides a framework for understanding mechanisms of fast inhibitory neurotransmission and allosteric modulation of Cys-loop receptors.

-	~~The entry 4tnv is ON HOLD until Paper Publication~~	+	Principles of activation and permeation in an anion-selective Cys-loop receptor.,Hibbs RE, Gouaux E Nature. 2011 May 15. PMID:21572436<ref>PMID:21572436</ref>

-	~~Authors~~: Althoff, T., ~~Hibbs, R.E~~., Gouaux, E., ~~Banerjee, S~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~:	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Mus musculus]]
		+	[[Category: Althoff, T.]]
		+	[[Category: Banerjee, S.]]
		+	[[Category: Gouaux, E.]]
		+	[[Category: Hibbs, R E.]]
		+	[[Category: Cys-loop receptor]]
		+	[[Category: Ligand-gated ion channel]]
		+	[[Category: Membrane protein]]
		+	[[Category: Neurotransmitter receptor]]
		+	[[Category: Transport protein-immune system complex]]

OCA at 09:53, 16 July 2014

2014-07-16T09:53:25Z

←Older revision		Revision as of 09:53, 16 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4tnv is ON HOLD	+	The entry 4tnv is ON HOLD until Paper Publication

	Authors: Althoff, T., Hibbs, R.E., Gouaux, E., Banerjee, S.		Authors: Althoff, T., Hibbs, R.E., Gouaux, E., Banerjee, S.

	Description:		Description:

OCA at 06:55, 9 July 2014

2014-07-09T06:55:24Z

←Older revision		Revision as of 06:55, 9 July 2014
Line 3:		Line 3:
	The entry 4tnv is ON HOLD		The entry 4tnv is ON HOLD

-	Authors: Althoff, T., Hibbs, R.E., Gouaux, E.	+	Authors: Althoff, T., Hibbs, R.E., Gouaux, E., Banerjee, S.

	Description:		Description: