4tpy - Revision history

OCA at 00:47, 28 December 2023

2023-12-28T00:47:21Z

←Older revision		Revision as of 00:47, 28 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tpy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TPY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tpy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TPY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tpy OCA], [https://pdbe.org/4tpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tpy RCSB], [https://www.ebi.ac.uk/pdbsum/4tpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tpy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tpy OCA], [https://pdbe.org/4tpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tpy RCSB], [https://www.ebi.ac.uk/pdbsum/4tpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tpy ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:48, 15 March 2023

2023-03-15T10:48:55Z

←Older revision		Revision as of 10:48, 15 March 2023
Line 3:		Line 3:
	<StructureSection load='4tpy' size='340' side='right'caption='[[4tpy]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='4tpy' size='340' side='right'caption='[[4tpy]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tpy]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TPY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4TPY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tpy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TPY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tpy OCA], [https://pdbe.org/4tpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tpy RCSB], [https://www.ebi.ac.uk/pdbsum/4tpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tpy ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4tpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tpy OCA], [~~http~~://pdbe.org/4tpy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4tpy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4tpy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4tpy ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 20:

	==See Also==		==See Also==
-	*[[Trypsin\|Trypsin]]	+	*[[Trypsin 3D structures\|Trypsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 27:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Trypsin]]~~	+	[[Category: Joshi K]]
-	[[Category: Joshi, K]]	+	[[Category: Mullen JD]]
-	[[Category: Mullen~~, J D~~]]	+	[[Category: Soares AS]]
-	[[Category: Soares~~, A S~~]]	+	[[Category: Teplitsky E]]
-	[[Category: Teplitsky, E]]	+
-	~~[[Category: Fibrin]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Serpin]]~~	+
-	~~[[Category: Thrombin~~]]	+

OCA at 08:37, 4 December 2019

2019-12-04T08:37:08Z

←Older revision		Revision as of 08:37, 4 December 2019
Line 1:		Line 1:

	==High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density==		==High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density==
-	<StructureSection load='4tpy' size='340' side='right' caption='[[4tpy]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='4tpy' size='340' side='right'caption='[[4tpy]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tpy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TPY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TPY FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tpy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TPY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TPY FirstGlance]. <br>
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 4tpy" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4tpy" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Trypsin\|Trypsin]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Large Structures]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
	[[Category: Joshi, K]]		[[Category: Joshi, K]]

OCA at 14:58, 15 November 2017

2017-11-15T14:58:05Z

←Older revision		Revision as of 14:58, 15 November 2017
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 4tpy" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4tpy" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Trypsin\|Trypsin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:24, 19 April 2017

2017-04-19T13:24:44Z

←Older revision		Revision as of 13:24, 19 April 2017
Line 1:		Line 1:
		+
	==High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density==		==High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density==
	<StructureSection load='4tpy' size='340' side='right' caption='[[4tpy]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='4tpy' size='340' side='right' caption='[[4tpy]], [[Resolution\|resolution]] 1.30Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tpy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tpy RCSB], [http://www.ebi.ac.uk/pdbsum/4tpy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tpy OCA], [http://pdbe.org/4tpy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tpy RCSB], [http://www.ebi.ac.uk/pdbsum/4tpy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tpy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4tpy" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 07:38, 15 July 2015

2015-07-15T07:38:30Z

←Older revision		Revision as of 07:38, 15 July 2015
Line 9:		Line 9:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	Acoustic droplet ejection (ADE) ~~is a powerful technology that supports crystallographic applications such as growing, improving and manipulating protein crystals. A fragment-screening strategy is described that uses ADE~~ to co-~~crystallize proteins with fragment libraries directly on MiTeGen MicroMeshes~~. ~~Co-crystallization trials can be prepared rapidly and economically. The high speed~~ of ~~specimen preparation and the low consumption of fragment and~~ protein ~~allow the use of individual rather than pooled fragments. The Echo 550 liquid-handling instrument (Labcyte Inc.~~, ~~Sunnyvale~~, ~~California, USA) generates droplets with accurate trajectories, which allows multiple~~ co-crystallization ~~experiments to be discretely positioned on~~ a ~~single data-collection micromesh. This accuracy also~~ allows all components to be transferred through small apertures~~. Consequently, the crystallization tray is~~ in ~~equilibrium with~~ the ~~reservoir before~~, ~~during and after~~ the ~~transfer of protein, precipitant and fragment to the micromesh on which crystallization will occur. This strict control of the specimen~~ environment ~~means that the crystallography experiments remain identical as the working volumes are decreased from the few microlitres level to~~ the ~~few nanolitres level~~. Using this system~~, lysozyme~~, thermolysin, trypsin ~~and stachydrine demethylase~~ crystals were ~~co-crystallized with~~ a ~~small 33~~-~~compound~~ mini-library to ~~search for~~ fragment ~~hits~~. This technology pushes towards a ~~much~~ faster, ~~more~~ automated and more flexible strategy for ~~structure-based drug discovery~~ using as little as 2.~~5 nl~~ of each ~~major~~ component.	+	We describe a high throughput method for screening up to 1728 distinct chemicals with protein crystals on a single microplate. Acoustic droplet ejection (ADE) was used to co-position 2.5nL of protein, precipitant, and chemicals on a MiTeGen in situ-1 crystallization plate for screening by co-crystallization or soaking. ADE-transferred droplets follow a precise trajectory which allows all components to be transferred through small apertures in the microplate lid. The apertures were large enough for 2.5nL droplets to pass through them, but small enough so that they did not disrupt the internal environment created by the mother liquor. Using this system, thermolysin and trypsin crystals were efficiently screened for binding to a heavy-metal mini-library. Fluorescence and X-ray diffraction were used to confirm that each chemical in the heavy-metal library was correctly paired with the intended protein crystal. A fragment mini-library was screened to observe two known lysozyme ligands using both co-crystallization and soaking. A similar approach was used to identify multiple, novel thaumatin binding sites for ascorbic acid. This technology pushes towards a faster, automated, and more flexible strategy for high throughput screening of chemical libraries (such as fragment libraries) using as little as 2.5nL of each component.

-	~~Hitting the target: fragment~~ screening ~~with~~ acoustic ~~in situ co-crystallization of proteins plus fragment~~ libraries on ~~pin-mounted data-collection micromeshes~~.,~~Yin X~~, ~~Scalia A, Leroy L, Cuttitta CM, Polizzo GM~~, Ericson DL, ~~Roessler CG~~, ~~Campos O, Ma MY, Agarwal R, Jackimowicz R, Allaire M, Orville AM~~, Sweet RM, Soares AS ~~Acta Crystallogr D~~ Biol ~~Crystallogr~~. ~~2014 May~~;70(~~Pt 5~~):~~1177~~-89. doi:, 10.~~1107~~/~~S1399004713034603~~. Epub ~~2014 Apr 30~~. PMID:~~24816088~~<ref>PMID:~~24816088~~</ref>	+	High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density.,Teplitsky E, Joshi K, Ericson DL, Scalia A, Mullen JD, Sweet RM, Soares AS J Struct Biol. 2015 Jul;191(1):49-58. doi: 10.1016/j.jsb.2015.05.006. Epub 2015, May 29. PMID:26027487<ref>PMID:26027487</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Trypsin\|Trypsin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:57, 25 January 2015

2015-01-25T07:57:14Z

←Older revision		Revision as of 07:57, 25 January 2015
Line 21:		Line 21:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
-	[[Category: Joshi, K.]]	+	[[Category: Joshi, K]]
-	[[Category: Mullen, J D.]]	+	[[Category: Mullen, J D]]
-	[[Category: Soares, A S.]]	+	[[Category: Soares, A S]]
-	[[Category: Teplitsky, E.]]	+	[[Category: Teplitsky, E]]
	[[Category: Fibrin]]		[[Category: Fibrin]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serpin]]		[[Category: Serpin]]
	[[Category: Thrombin]]		[[Category: Thrombin]]

OCA at 11:48, 22 October 2014

2014-10-22T11:48:44Z

←Older revision		Revision as of 11:48, 22 October 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density==
		+	<StructureSection load='4tpy' size='340' side='right' caption='[[4tpy]], [[Resolution\|resolution]] 1.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4tpy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TPY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TPY FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tpy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tpy RCSB], [http://www.ebi.ac.uk/pdbsum/4tpy PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acoustic droplet ejection (ADE) is a powerful technology that supports crystallographic applications such as growing, improving and manipulating protein crystals. A fragment-screening strategy is described that uses ADE to co-crystallize proteins with fragment libraries directly on MiTeGen MicroMeshes. Co-crystallization trials can be prepared rapidly and economically. The high speed of specimen preparation and the low consumption of fragment and protein allow the use of individual rather than pooled fragments. The Echo 550 liquid-handling instrument (Labcyte Inc., Sunnyvale, California, USA) generates droplets with accurate trajectories, which allows multiple co-crystallization experiments to be discretely positioned on a single data-collection micromesh. This accuracy also allows all components to be transferred through small apertures. Consequently, the crystallization tray is in equilibrium with the reservoir before, during and after the transfer of protein, precipitant and fragment to the micromesh on which crystallization will occur. This strict control of the specimen environment means that the crystallography experiments remain identical as the working volumes are decreased from the few microlitres level to the few nanolitres level. Using this system, lysozyme, thermolysin, trypsin and stachydrine demethylase crystals were co-crystallized with a small 33-compound mini-library to search for fragment hits. This technology pushes towards a much faster, more automated and more flexible strategy for structure-based drug discovery using as little as 2.5 nl of each major component.

-	~~The entry 4tpy is ON HOLD until Paper Publication~~	+	Hitting the target: fragment screening with acoustic in situ co-crystallization of proteins plus fragment libraries on pin-mounted data-collection micromeshes.,Yin X, Scalia A, Leroy L, Cuttitta CM, Polizzo GM, Ericson DL, Roessler CG, Campos O, Ma MY, Agarwal R, Jackimowicz R, Allaire M, Orville AM, Sweet RM, Soares AS Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1177-89. doi:, 10.1107/S1399004713034603. Epub 2014 Apr 30. PMID:24816088<ref>PMID:24816088</ref>

-	~~Authors: Teplitsky~~, E., Joshi, K., Mullen, J.D., Soares, A.S.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bos taurus]]
		+	[[Category: Trypsin]]
		+	[[Category: Joshi, K.]]
		+	[[Category: Mullen, J D.]]
		+	[[Category: Soares, A S.]]
		+	[[Category: Teplitsky, E.]]
		+	[[Category: Fibrin]]
		+	[[Category: Hydrolase]]
		+	[[Category: Serpin]]
		+	[[Category: Thrombin]]

OCA at 06:02, 25 June 2014

2014-06-25T06:02:15Z

←Older revision		Revision as of 06:02, 25 June 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4tpy is ON HOLD	+	The entry 4tpy is ON HOLD until Paper Publication

	Authors: Teplitsky, E., Joshi, K., Mullen, J.D., Soares, A.S.		Authors: Teplitsky, E., Joshi, K., Mullen, J.D., Soares, A.S.

	Description: High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density		Description: High throughput screening using acoustic droplet ejection to combine protein crystals and chemical libraries on crystallization plates at high density

OCA: Protected "4tpy" [edit=sysop:move=sysop]

2014-06-18T06:47:04Z

Protected "4tpy" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:47, 18 June 2014