4tsl - Revision history

OCA at 15:17, 8 November 2023

2023-11-08T15:17:24Z

←Older revision		Revision as of 15:17, 8 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tsl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinia_fragacea Actinia fragacea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TSL FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tsl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinia_fragacea Actinia fragacea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TSL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsl OCA], [https://pdbe.org/4tsl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tsl RCSB], [https://www.ebi.ac.uk/pdbsum/4tsl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tsl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsl OCA], [https://pdbe.org/4tsl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tsl RCSB], [https://www.ebi.ac.uk/pdbsum/4tsl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tsl ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:53, 15 March 2023

2023-03-15T10:53:38Z

←Older revision		Revision as of 10:53, 15 March 2023
Line 3:		Line 3:
	<StructureSection load='4tsl' size='340' side='right'caption='[[4tsl]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4tsl' size='340' side='right'caption='[[4tsl]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tsl]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Actfr Actfr~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4TSL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tsl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinia_fragacea Actinia fragacea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TSL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vwi\|3vwi]], [[3w9p\|3w9p]], [[4tsn\|4tsn]], [[4tso\|4tso]], [[4tsp\|4tsp]], [[4tsq\|4tsq]], [[4tsy\|4tsy]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsl OCA], [https://pdbe.org/4tsl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tsl RCSB], [https://www.ebi.ac.uk/pdbsum/4tsl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tsl ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4tsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsl OCA], [~~http~~://pdbe.org/4tsl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4tsl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4tsl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4tsl ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ACTPC_ACTFR ACTPC_ACTFR]] Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and hemolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of several monomers.<ref>PMID:19563820</ref>	+	[https://www.uniprot.org/uniprot/ACTPC_ACTFR ACTPC_ACTFR] Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and hemolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of several monomers.<ref>PMID:19563820</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Actfr~~]]	+	[[Category: Actinia fragacea]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Caaveiro~~, J M.M~~]]	+	[[Category: Caaveiro JMM]]
-	[[Category: Tanaka, K]]	+	[[Category: Tanaka K]]
-	[[Category: Tsumoto, K]]	+	[[Category: Tsumoto K]]
-	~~[[Category: Actinoporin]]~~	+
-	~~[[Category: Lipid-protein interaction]]~~	+
-	~~[[Category: Membrane lipid]]~~	+
-	~~[[Category: Phosphocholine]]~~	+
-	~~[[Category: Pore-forming toxin]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 15:56, 29 January 2020

2020-01-29T15:56:29Z

←Older revision		Revision as of 15:56, 29 January 2020
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4tsl" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4tsl" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytolysin 3D structures\|Cytolysin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:08, 10 May 2019

2019-05-10T14:08:03Z

←Older revision		Revision as of 14:08, 10 May 2019
Line 1:		Line 1:

	==Crystal structure of FraC with POC bound (crystal form I)==		==Crystal structure of FraC with POC bound (crystal form I)==
-	<StructureSection load='4tsl' size='340' side='right' caption='[[4tsl]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='4tsl' size='340' side='right'caption='[[4tsl]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tsl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TSL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tsl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Actfr Actfr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TSL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vwi\|3vwi]], [[3w9p\|3w9p]], [[4tsn\|4tsn]], [[4tso\|4tso]], [[4tsp\|4tsp]], [[4tsq\|4tsq]], [[4tsy\|4tsy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vwi\|3vwi]], [[3w9p\|3w9p]], [[4tsn\|4tsn]], [[4tso\|4tso]], [[4tsp\|4tsp]], [[4tsq\|4tsq]], [[4tsy\|4tsy]]</td></tr>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Actfr]]
		+	[[Category: Large Structures]]
	[[Category: Caaveiro, J M.M]]		[[Category: Caaveiro, J M.M]]
	[[Category: Tanaka, K]]		[[Category: Tanaka, K]]

OCA at 20:42, 3 August 2016

2016-08-03T20:42:54Z

←Older revision		Revision as of 20:42, 3 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of FraC with POC bound (crystal form I)==		==Crystal structure of FraC with POC bound (crystal form I)==
	<StructureSection load='4tsl' size='340' side='right' caption='[[4tsl]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4tsl' size='340' side='right' caption='[[4tsl]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vwi\|3vwi]], [[3w9p\|3w9p]], [[4tsn\|4tsn]], [[4tso\|4tso]], [[4tsp\|4tsp]], [[4tsq\|4tsq]], [[4tsy\|4tsy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vwi\|3vwi]], [[3w9p\|3w9p]], [[4tsn\|4tsn]], [[4tso\|4tso]], [[4tsp\|4tsp]], [[4tsq\|4tsq]], [[4tsy\|4tsy]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tsl RCSB], [http://www.ebi.ac.uk/pdbsum/4tsl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsl OCA], [http://pdbe.org/4tsl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tsl RCSB], [http://www.ebi.ac.uk/pdbsum/4tsl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tsl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	~~Actinia fragacea is commonly called the "strawberry" anemone because of the distinctive yellow or green spots displayed on its red column. Its venom contains several haemolytic~~ proteins ~~with a molecular mass of approximately 20 kDa~~ that ~~can be separated by ion~~-~~exchange column chromatography~~. ~~One~~ of ~~them was purified to homogeneity and was named~~ fragaceatoxin C (FraC)~~. Its 15 N~~-~~terminal residues were identified~~ by ~~Edman degradation and served to obtain its complete DNA coding sequence by RT-PCR. The coding region~~ of FraC ~~was amplified and cloned in~~ the ~~expression vector pBAT~~-4. ~~Purified recombinant FraC consists~~ of ~~179 amino acids~~ and ~~multiple sequence alignment~~ with ~~other actinoporins clearly indicates~~ that ~~FraC belongs~~ to ~~this protein family~~. The ~~secondary~~ structure~~, thermal stability and lytic activity~~ of ~~native and recombinant FraC were practically identical and exhibit~~ the ~~same basic features already described for equinatoxin~~-~~II and sticholysin-II~~.	+	Pore-forming toxins (PFT) are water-soluble proteins that possess the remarkable ability to self-assemble on the membrane of target cells, where they form pores causing cell damage. Here, we elucidate the mechanism of action of the haemolytic protein fragaceatoxin C (FraC), a alpha-barrel PFT, by determining the crystal structures of FraC at four different stages of the lytic mechanism, namely the water-soluble state, the monomeric lipid-bound form, an assembly intermediate and the fully assembled transmembrane pore. The structure of the transmembrane pore exhibits a unique architecture composed of both protein and lipids, with some of the lipids lining the pore wall, acting as assembly cofactors. The pore also exhibits lateral fenestrations that expose the hydrophobic core of the membrane to the aqueous environment. The incorporation of lipids from the target membrane within the structure of the pore provides a membrane-specific trigger for the activation of a haemolytic toxin.

-	~~Purification, cloning and characterization~~ of ~~fragaceatoxin C,~~ a ~~novel actinoporin from the sea anemone Actinia fragacea~~.,~~Bellomio A~~, Morante K~~, Barlic A, Gutierrez-Aguirre I, Viguera AR~~, Gonzalez-Manas JM ~~Toxicon~~. ~~2009 Nov~~;~~54(~~6):~~869-80~~. doi: 10.~~1016~~/~~j.toxicon.2009.06.022. Epub 2009 Jun, 27~~. PMID:~~19563820~~<ref>PMID:~~19563820~~</ref>	+	Structural basis for self-assembly of a cytolytic pore lined by protein and lipid.,Tanaka K, Caaveiro JM, Morante K, Gonzalez-Manas JM, Tsumoto K Nat Commun. 2015 Feb 26;6:6337. doi: 10.1038/ncomms7337. PMID:25716479<ref>PMID:25716479</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4tsl" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:26, 4 March 2015

2015-03-04T11:26:31Z

←Older revision		Revision as of 11:26, 4 March 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of FraC with POC bound (crystal form I)==
		+	<StructureSection load='4tsl' size='340' side='right' caption='[[4tsl]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4tsl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TSL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TSL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3vwi\|3vwi]], [[3w9p\|3w9p]], [[4tsn\|4tsn]], [[4tso\|4tso]], [[4tsp\|4tsp]], [[4tsq\|4tsq]], [[4tsy\|4tsy]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tsl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tsl RCSB], [http://www.ebi.ac.uk/pdbsum/4tsl PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACTPC_ACTFR ACTPC_ACTFR]] Pore-forming protein that forms cations-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and hemolysis. Pore formation is a multi-step process that involves specific recognition of membrane sphingomyelin (but neither cholesterol nor phosphatidylcholine) using aromatic rich region and adjacent phosphocholine (POC) binding site, firm binding to the membrane (mainly driven by hydrophobic interactions) accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of several monomers.<ref>PMID:19563820</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Actinia fragacea is commonly called the "strawberry" anemone because of the distinctive yellow or green spots displayed on its red column. Its venom contains several haemolytic proteins with a molecular mass of approximately 20 kDa that can be separated by ion-exchange column chromatography. One of them was purified to homogeneity and was named fragaceatoxin C (FraC). Its 15 N-terminal residues were identified by Edman degradation and served to obtain its complete DNA coding sequence by RT-PCR. The coding region of FraC was amplified and cloned in the expression vector pBAT-4. Purified recombinant FraC consists of 179 amino acids and multiple sequence alignment with other actinoporins clearly indicates that FraC belongs to this protein family. The secondary structure, thermal stability and lytic activity of native and recombinant FraC were practically identical and exhibit the same basic features already described for equinatoxin-II and sticholysin-II.

-	~~The entry 4tsl is ON HOLD until Paper Publication~~	+	Purification, cloning and characterization of fragaceatoxin C, a novel actinoporin from the sea anemone Actinia fragacea.,Bellomio A, Morante K, Barlic A, Gutierrez-Aguirre I, Viguera AR, Gonzalez-Manas JM Toxicon. 2009 Nov;54(6):869-80. doi: 10.1016/j.toxicon.2009.06.022. Epub 2009 Jun, 27. PMID:19563820<ref>PMID:19563820</ref>

-	~~Authors: Caaveiro~~, J.M.~~M., Tanaka, K., Tsumoto, K~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of FraC with POC bound (crystal form I)~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
-	[[Category: Caaveiro, J.M.M]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Caaveiro, J M.M]]
	[[Category: Tanaka, K]]		[[Category: Tanaka, K]]
	[[Category: Tsumoto, K]]		[[Category: Tsumoto, K]]
		+	[[Category: Actinoporin]]
		+	[[Category: Lipid-protein interaction]]
		+	[[Category: Membrane lipid]]
		+	[[Category: Phosphocholine]]
		+	[[Category: Pore-forming toxin]]
		+	[[Category: Toxin]]

OCA at 17:50, 24 December 2014

2014-12-24T17:50:39Z

←Older revision		Revision as of 17:50, 24 December 2014
Line 6:		Line 6:

	Description: Crystal structure of FraC with POC bound (crystal form I)		Description: Crystal structure of FraC with POC bound (crystal form I)
		+	[[Category: Unreleased Structures]]
		+	[[Category: Caaveiro, J.M.M]]
		+	[[Category: Tanaka, K]]
		+	[[Category: Tsumoto, K]]

OCA at 09:55, 16 July 2014

2014-07-16T09:55:03Z

←Older revision		Revision as of 09:55, 16 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4tsl is ON HOLD	+	The entry 4tsl is ON HOLD until Paper Publication

	Authors: Caaveiro, J.M.M., Tanaka, K., Tsumoto, K.		Authors: Caaveiro, J.M.M., Tanaka, K., Tsumoto, K.

	Description: Crystal structure of FraC with POC bound (crystal form I)		Description: Crystal structure of FraC with POC bound (crystal form I)

OCA: Protected "4tsl" [edit=sysop:move=sysop]

2014-06-25T06:05:03Z

Protected "4tsl" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:05, 25 June 2014

OCA: New page: '''Unreleased structure''' The entry 4tsl is ON HOLD Authors: Caaveiro, J.M.M., Tanaka, K., Tsumoto, K. Description: Crystal structure of FraC with POC bound (crystal form I)

2014-06-25T06:05:01Z

New page: '''Unreleased structure''' The entry 4tsl is ON HOLD Authors: Caaveiro, J.M.M., Tanaka, K., Tsumoto, K. Description: Crystal structure of FraC with POC bound (crystal form I)

New page

'''Unreleased structure'''

The entry 4tsl is ON HOLD

Authors: Caaveiro, J.M.M., Tanaka, K., Tsumoto, K.

Description: Crystal structure of FraC with POC bound (crystal form I)