4tt9 - Revision history

OCA at 12:24, 20 December 2023

2023-12-20T12:24:34Z

←Older revision		Revision as of 12:24, 20 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tt9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TT9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tt9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TT9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tt9 OCA], [https://pdbe.org/4tt9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tt9 RCSB], [https://www.ebi.ac.uk/pdbsum/4tt9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tt9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tt9 OCA], [https://pdbe.org/4tt9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tt9 RCSB], [https://www.ebi.ac.uk/pdbsum/4tt9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tt9 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:54, 15 March 2023

2023-03-15T10:54:33Z

←Older revision		Revision as of 10:54, 15 March 2023
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	<StructureSection load='4tt9' size='340' side='right'caption='[[4tt9]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4tt9' size='340' side='right'caption='[[4tt9]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tt9]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TT9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4TT9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tt9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TT9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TT9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spaO, spa33, CP0152 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tt9 OCA], [https://pdbe.org/4tt9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tt9 RCSB], [https://www.ebi.ac.uk/pdbsum/4tt9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tt9 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4tt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tt9 OCA], [~~http~~://pdbe.org/4tt9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4tt9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4tt9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4tt9 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SPAO_SHIFL SPAO_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the Ipa antigens. Required for invasion and for secretion of the three Ipa proteins.	+	[https://www.uniprot.org/uniprot/SPAO_SHIFL SPAO_SHIFL] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the Ipa antigens. Required for invasion and for secretion of the three Ipa proteins.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Shigella paradysenteriae weldin 1927]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Johnson, S~~]]	+	[[Category: Shigella flexneri]]
-	[[Category: ~~Lea,~~ S M]]	+	[[Category: Johnson S]]
-	[[Category: ~~McDowell, M A~~]]	+	[[Category: Lea SM]]
-	[[Category: ~~C-ring]]~~	+	[[Category: McDowell MA]]
-	~~[[Category: Flin]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Spoa]]~~	+
-	~~[[Category: T3ss~~]]	+

OCA at 14:24, 10 May 2019

2019-05-10T14:24:10Z

←Older revision		Revision as of 14:24, 10 May 2019
Line 1:		Line 1:

	==Structure of the C-terminal SpoA domain of Shigella flexneri Spa33==		==Structure of the C-terminal SpoA domain of Shigella flexneri Spa33==
-	<StructureSection load='4tt9' size='340' side='right' caption='[[4tt9]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='4tt9' size='340' side='right'caption='[[4tt9]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tt9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TT9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tt9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TT9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tt9 OCA], [http://pdbe.org/4tt9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tt9 RCSB], [http://www.ebi.ac.uk/pdbsum/4tt9 PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spaO, spa33, CP0152 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tt9 OCA], [http://pdbe.org/4tt9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tt9 RCSB], [http://www.ebi.ac.uk/pdbsum/4tt9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tt9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Shigella paradysenteriae weldin 1927]]
		+	[[Category: Large Structures]]
	[[Category: Johnson, S]]		[[Category: Johnson, S]]
	[[Category: Lea, S M]]		[[Category: Lea, S M]]

OCA at 10:17, 26 February 2016

2016-02-26T10:17:34Z

←Older revision		Revision as of 10:17, 26 February 2016
Line 1:		Line 1:
		+
	==Structure of the C-terminal SpoA domain of Shigella flexneri Spa33==		==Structure of the C-terminal SpoA domain of Shigella flexneri Spa33==
	<StructureSection load='4tt9' size='340' side='right' caption='[[4tt9]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4tt9' size='340' side='right' caption='[[4tt9]], [[Resolution\|resolution]] 2.30Å' scene=''>

OCA at 19:44, 30 November 2015

2015-11-30T19:44:41Z

←Older revision		Revision as of 19:44, 30 November 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[4tt9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TT9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tt9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TT9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tt9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tt9 RCSB], [http://www.ebi.ac.uk/pdbsum/4tt9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tt9 OCA], [http://pdbe.org/4tt9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tt9 RCSB], [http://www.ebi.ac.uk/pdbsum/4tt9 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SPAO_SHIFL SPAO_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the Ipa antigens. Required for invasion and for secretion of the three Ipa proteins.		[[http://www.uniprot.org/uniprot/SPAO_SHIFL SPAO_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the Ipa antigens. Required for invasion and for secretion of the three Ipa proteins.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Flagellar Type III secretion systems (T3SS) contain an essential Cytoplasmic-ring (C-ring) largely composed of two proteins FliM and FliN, whilst an analogous substructure for the closely related non-flagellar (NF) T3SS has not been observed in situ. We show that the spa33 gene encoding the putative NF-T3SS C-ring component in Shigella flexneri is alternatively translated to produce both full-length (Spa33-FL) and a short variant (Spa33-C), with both required for secretion. They associate in a 1:2 complex (Spa33-FL/C2 ) that further oligomerises into elongated arrays in vitro. The structure of Spa33-C2 and identification of an unexpected intramolecular pseudodimer in Spa33-FL, reveals a molecular model for their higher order assembly within NF-T3SS. Spa33-FL and Spa33-C are identified as functional counterparts of a FliM-FliN fusion and free FliN respectively. Furthermore, we show that Thermotoga maritima FliM and FliN form a 1:3 complex structurally equivalent to Spa33-FL/C2 , allowing us to propose a unified model for C-ring assembly by NF-T3SS and flagellar-T3SS.
		+
		+	Characterisation of Shigella Spa33 and Thermotoga FliM/N reveals a new model for C-ring assembly in T3SS.,McDowell MA, Marcoux J, McVicker G, Johnson S, Fong YH, Stevens R, Bowman LA, Degiacomi MT, Yan J, Wise A, Friede M, Benesch JL, Deane JE, Tang CM, Robinson CV, Lea SM Mol Microbiol. 2015 Nov 5. doi: 10.1111/mmi.13267. PMID:26538516<ref>PMID:26538516</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4tt9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:00, 24 June 2015

2015-06-24T13:00:06Z

←Older revision		Revision as of 13:00, 24 June 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Structure of the C-terminal SpoA domain of Shigella flexneri Spa33==
-		+	<StructureSection load='4tt9' size='340' side='right' caption='[[4tt9]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~The entry~~ 4tt9 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4tt9]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TT9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TT9 FirstGlance]. <br>
-	~~Authors~~: ~~McDowell~~, M.A., ~~Johnson, S~~., ~~Lea, S~~.M.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tt9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tt9 RCSB], [http://www.ebi.ac.uk/pdbsum/4tt9 PDBsum]</span></td></tr>
-	~~Description: Structure of the C-terminal SpoA domain of Shigella flexneri Spa33~~	+	</table>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPAO_SHIFL SPAO_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the Ipa antigens. Required for invasion and for secretion of the three Ipa proteins.
		+	__TOC__
		+	</StructureSection>
	[[Category: Johnson, S]]		[[Category: Johnson, S]]
-	[[Category: Lea, S.M]]	+	[[Category: Lea, S M]]
-	[[Category: ~~Mcdowell~~, M.A]]	+	[[Category: McDowell, M A]]
		+	[[Category: C-ring]]
		+	[[Category: Flin]]
		+	[[Category: Immune system]]
		+	[[Category: Protein transport]]
		+	[[Category: Spoa]]
		+	[[Category: T3ss]]

OCA at 17:51, 24 December 2014

2014-12-24T17:51:23Z

←Older revision		Revision as of 17:51, 24 December 2014
Line 6:		Line 6:

	Description: Structure of the C-terminal SpoA domain of Shigella flexneri Spa33		Description: Structure of the C-terminal SpoA domain of Shigella flexneri Spa33
		+	[[Category: Unreleased Structures]]
		+	[[Category: Johnson, S]]
		+	[[Category: Lea, S.M]]
		+	[[Category: Mcdowell, M.A]]

OCA at 01:21, 7 August 2014

2014-08-07T01:21:02Z

←Older revision		Revision as of 01:21, 7 August 2014
Line 3:		Line 3:
	The entry 4tt9 is ON HOLD until Paper Publication		The entry 4tt9 is ON HOLD until Paper Publication

-	Authors: McDowell, M.A., Johnson, S, Lea, S.M.	+	Authors: McDowell, M.A., Johnson, S., Lea, S.M.

	Description: Structure of the C-terminal SpoA domain of Shigella flexneri Spa33		Description: Structure of the C-terminal SpoA domain of Shigella flexneri Spa33

OCA: Protected "4tt9" [edit=sysop:move=sysop]

2014-07-02T07:33:10Z

Protected "4tt9" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:33, 2 July 2014

OCA: New page: '''Unreleased structure''' The entry 4tt9 is ON HOLD until Paper Publication Authors: McDowell, M.A., Johnson, S, Lea, S.M. Description: Structure of the C-terminal SpoA domain of Shig...

2014-07-02T07:33:09Z

New page: '''Unreleased structure''' The entry 4tt9 is ON HOLD until Paper Publication Authors: McDowell, M.A., Johnson, S, Lea, S.M. Description: Structure of the C-terminal SpoA domain of Shig...

New page

'''Unreleased structure'''

The entry 4tt9 is ON HOLD until Paper Publication

Authors: McDowell, M.A., Johnson, S, Lea, S.M.

Description: Structure of the C-terminal SpoA domain of Shigella flexneri Spa33