4tx4 - Revision history

OCA at 00:48, 28 December 2023

2023-12-28T00:48:27Z

←Older revision		Revision as of 00:48, 28 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4tx4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vigna_unguiculata Vigna unguiculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TX4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tx4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vigna_unguiculata Vigna unguiculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TX4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tx4 OCA], [https://pdbe.org/4tx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tx4 RCSB], [https://www.ebi.ac.uk/pdbsum/4tx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tx4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tx4 OCA], [https://pdbe.org/4tx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tx4 RCSB], [https://www.ebi.ac.uk/pdbsum/4tx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tx4 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:50, 22 March 2023

2023-03-22T07:50:31Z

←Older revision		Revision as of 07:50, 22 March 2023
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	<StructureSection load='4tx4' size='340' side='right'caption='[[4tx4]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='4tx4' size='340' side='right'caption='[[4tx4]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tx4]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cowpea Cowpea~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TX4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4TX4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tx4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vigna_unguiculata Vigna unguiculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TX4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4tx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tx4 OCA], [~~http~~://pdbe.org/4tx4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4tx4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4tx4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4tx4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tx4 OCA], [https://pdbe.org/4tx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tx4 RCSB], [https://www.ebi.ac.uk/pdbsum/4tx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tx4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CYTI_VIGUN CYTI_VIGUN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cowpea]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Carvalho~~, C P.S~~]]	+	[[Category: Vigna unguiculata]]
-	[[Category: Grangeiro~~, T B~~]]	+	[[Category: Carvalho CPS]]
-	[[Category: Monteiro-Junior~~, J E~~]]	+	[[Category: Grangeiro TB]]
-	[[Category: Pereira~~, H M~~]]	+	[[Category: Monteiro-Junior JE]]
-	[[Category: Valadares, N]]	+	[[Category: Pereira HM]]
-	~~[[Category: Cysteine protease inhibitor]]~~	+	[[Category: Valadares N]]
-	~~[[Category: Hydrolase inhibitor~~]]	+

OCA at 06:16, 17 April 2019

2019-04-17T06:16:43Z

←Older revision		Revision as of 06:16, 17 April 2019
Line 1:		Line 1:

	==Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)==		==Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)==
-	<StructureSection load='4tx4' size='340' side='right' caption='[[4tx4]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='4tx4' size='340' side='right'caption='[[4tx4]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4tx4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TX4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4tx4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cowpea Cowpea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TX4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tx4 OCA], [http://pdbe.org/4tx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tx4 RCSB], [http://www.ebi.ac.uk/pdbsum/4tx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tx4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tx4 OCA], [http://pdbe.org/4tx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tx4 RCSB], [http://www.ebi.ac.uk/pdbsum/4tx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tx4 ProSAT]</span></td></tr>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cowpea]]
		+	[[Category: Large Structures]]
	[[Category: Carvalho, C P.S]]		[[Category: Carvalho, C P.S]]
	[[Category: Grangeiro, T B]]		[[Category: Grangeiro, T B]]

OCA at 09:46, 3 August 2017

2017-08-03T09:46:00Z

←Older revision		Revision as of 09:46, 3 August 2017
Line 1:		Line 1:
		+
	==Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)==		==Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)==
	<StructureSection load='4tx4' size='340' side='right' caption='[[4tx4]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='4tx4' size='340' side='right' caption='[[4tx4]], [[Resolution\|resolution]] 1.95Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4tx4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TX4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4tx4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TX4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tx4 OCA], [http://pdbe.org/4tx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tx4 RCSB], [http://www.ebi.ac.uk/pdbsum/4tx4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tx4 OCA], [http://pdbe.org/4tx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tx4 RCSB], [http://www.ebi.ac.uk/pdbsum/4tx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tx4 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Two cysteine proteinase inhibitors from cowpea, VuCys1 and VuCys2, were produced in E. coli ArcticExpress (DE3). The recombinant products strongly inhibited papain and chymopapain as well as the midgut proteases from Callosobruchus maculatus larvae, a bruchid that uses cysteine proteases as major digestive enzymes. Heat treatment at 100 degrees C for up to 60min or incubation at various pH values caused little reduction in the papain inhibitory activity of both inhibitors. Moreover, minor conformational variations, as probed by circular dichroism spectroscopy, were observed after VuCys1 and VuCys2 were subjected to these treatments. The crystal structure of VuCys1 was determined at a resolution of 1.95A, revealing a domain-swapped dimer in the asymmetric unit. However, the two lobes of the domain-swapped dimer are positioned closer to each other in VuCys1 in comparison to other similar cystatin structures. Moreover, some polar residues from opposite lobes recruit water molecules, forming a hydrogen bond network that mediates contacts between the lobes, thus generating an extended open interface. Due to the closer distance between the lobes, a small hydrophobic core is also formed, further stabilizing the folded domain-swapped dimer. These structural features might account for the extraordinary thermal and pH stability of VuCys1.
		+
		+	Expression in Escherichia coli of cysteine protease inhibitors from cowpea (Vigna unguiculata): The crystal structure of a single-domain cystatin gives insights on its thermal and pH stability.,Monteiro Junior JE, Valadares NF, Pereira HD, Dyszy FH, da Costa Filho AJ, Uchoa AF, de Oliveira AS, da Silveira Carvalho CP, Grangeiro TB Int J Biol Macromol. 2017 Sep;102:29-41. doi: 10.1016/j.ijbiomac.2017.04.008., Epub 2017 Apr 4. PMID:28389401<ref>PMID:28389401</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4tx4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:02, 16 October 2015

2015-10-16T04:02:11Z

←Older revision		Revision as of 04:02, 16 October 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)==
-		+	<StructureSection load='4tx4' size='340' side='right' caption='[[4tx4]], [[Resolution\|resolution]] 1.95Å' scene=''>
-	~~The entry~~ 4tx4 is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4tx4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TX4 FirstGlance]. <br>
-	~~Authors~~: ~~Pereira, H~~.M.~~, Valadares, N~~.~~, Monteiro~~-~~Junior~~, J.E., ~~Carvalho~~, C.P.S., ~~Grangeiro, T~~.B.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tx4 OCA], [http://pdbe.org/4tx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tx4 RCSB], [http://www.ebi.ac.uk/pdbsum/4tx4 PDBsum]</span></td></tr>
-	~~Description~~: ~~Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)~~	+	</table>
-	[[Category: ~~Unreleased Structures~~]]	+	__TOC__
-	[[Category: ~~Pereira~~, ~~H.M~~]]	+	</StructureSection>
-	[[Category: ~~Carvalho~~, ~~C.P.S~~]]	+	[[Category: Carvalho, C P.S]]
		+	[[Category: Grangeiro, T B]]
		+	[[Category: Monteiro-Junior, J E]]
		+	[[Category: Pereira, H M]]
	[[Category: Valadares, N]]		[[Category: Valadares, N]]
-	[[Category: ~~Grangeiro, T.B~~]]	+	[[Category: Cysteine protease inhibitor]]
-	[[Category: ~~Monteiro-Junior, J.E~~]]	+	[[Category: Hydrolase inhibitor]]

OCA at 17:56, 24 December 2014

2014-12-24T17:56:10Z

←Older revision		Revision as of 17:56, 24 December 2014
Line 6:		Line 6:

	Description: Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)		Description: Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)
		+	[[Category: Unreleased Structures]]
		+	[[Category: Pereira, H.M]]
		+	[[Category: Carvalho, C.P.S]]
		+	[[Category: Valadares, N]]
		+	[[Category: Grangeiro, T.B]]
		+	[[Category: Monteiro-Junior, J.E]]

OCA at 08:41, 24 September 2014

2014-09-24T08:41:41Z

←Older revision		Revision as of 08:41, 24 September 2014
Line 3:		Line 3:
	The entry 4tx4 is ON HOLD until Paper Publication		The entry 4tx4 is ON HOLD until Paper Publication

-	Authors: Pereira, H.M, Valadares, N., Monteiro-Junior, J.E., Carvalho, C.P.S., Grangeiro, T.B.	+	Authors: Pereira, H.M., Valadares, N., Monteiro-Junior, J.E., Carvalho, C.P.S., Grangeiro, T.B.

	Description: Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)		Description: Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)

OCA at 07:21, 23 July 2014

2014-07-23T07:21:48Z

←Older revision		Revision as of 07:21, 23 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4tx4 is ON HOLD	+	The entry 4tx4 is ON HOLD until Paper Publication

	Authors: Pereira, H.M, Valadares, N., Monteiro-Junior, J.E., Carvalho, C.P.S., Grangeiro, T.B.		Authors: Pereira, H.M, Valadares, N., Monteiro-Junior, J.E., Carvalho, C.P.S., Grangeiro, T.B.

	Description: Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)		Description: Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)

OCA: Protected "4tx4" [edit=sysop:move=sysop]

2014-07-09T07:01:02Z

Protected "4tx4" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:01, 9 July 2014

OCA: New page: '''Unreleased structure''' The entry 4tx4 is ON HOLD Authors: Pereira, H.M, Valadares, N., Monteiro-Junior, J.E., Carvalho, C.P.S., Grangeiro, T.B. Description: Crystal Structure of a ...

2014-07-09T07:01:01Z

New page: '''Unreleased structure''' The entry 4tx4 is ON HOLD Authors: Pereira, H.M, Valadares, N., Monteiro-Junior, J.E., Carvalho, C.P.S., Grangeiro, T.B. Description: Crystal Structure of a ...

New page

'''Unreleased structure'''

The entry 4tx4 is ON HOLD

Authors: Pereira, H.M, Valadares, N., Monteiro-Junior, J.E., Carvalho, C.P.S., Grangeiro, T.B.

Description: Crystal Structure of a Single-Domain Cysteine Protease Inhibitor from Cowpea (Vigna unguiculata)