4uhc - Revision history

OCA at 10:29, 10 January 2024

2024-01-10T10:29:26Z

←Older revision		Revision as of 10:29, 10 January 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4uhc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermogutta_terrifontis Thermogutta terrifontis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UHC FirstGlance]. <br>		<table><tr><td colspan='2'>[[4uhc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermogutta_terrifontis Thermogutta terrifontis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UHC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.03Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhc OCA], [https://pdbe.org/4uhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uhc RCSB], [https://www.ebi.ac.uk/pdbsum/4uhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhc OCA], [https://pdbe.org/4uhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uhc RCSB], [https://www.ebi.ac.uk/pdbsum/4uhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhc ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:24, 22 March 2023

2023-03-22T08:24:04Z

←Older revision		Revision as of 08:24, 22 March 2023
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	<StructureSection load='4uhc' size='340' side='right'caption='[[4uhc]], [[Resolution\|resolution]] 1.03Å' scene=''>		<StructureSection load='4uhc' size='340' side='right'caption='[[4uhc]], [[Resolution\|resolution]] 1.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4uhc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Dsm_26237 Dsm 26237~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4UHC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4uhc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermogutta_terrifontis Thermogutta terrifontis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UHC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhd\|4uhd]], [[4uhe\|4uhe]], [[4uhf\|4uhf]], [[4uhh\|4uhh]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhc OCA], [https://pdbe.org/4uhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uhc RCSB], [https://www.ebi.ac.uk/pdbsum/4uhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhc ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4uhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhc OCA], [~~http~~://pdbe.org/4uhc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4uhc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4uhc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhc ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0M3KKY6_9BACT A0A0M3KKY6_9BACT]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Carboxylesterase]]
-	[[Category: Dsm 26237]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bonch-Osmolovskaya, E]]	+	[[Category: Thermogutta terrifontis]]
-	[[Category: Isupov~~, M N~~]]	+	[[Category: Bonch-Osmolovskaya E]]
-	[[Category: Littlechild~~, J A~~]]	+	[[Category: Isupov MN]]
-	[[Category: Sayer, C]]	+	[[Category: Littlechild JA]]
-	~~[[Category: Alpha beta hydrolase]]~~	+	[[Category: Sayer C]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 08:41, 21 May 2019

2019-05-21T08:41:40Z

←Older revision		Revision as of 08:41, 21 May 2019
Line 1:		Line 1:

	==Structural studies of a thermophilic esterase from Thermogutta terrifontis (Native)==		==Structural studies of a thermophilic esterase from Thermogutta terrifontis (Native)==
-	<StructureSection load='4uhc' size='340' side='right' caption='[[4uhc]], [[Resolution\|resolution]] 1.03Å' scene=''>	+	<StructureSection load='4uhc' size='340' side='right'caption='[[4uhc]], [[Resolution\|resolution]] 1.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4uhc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UHC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4uhc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_26237 Dsm 26237]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UHC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhd\|4uhd]], [[4uhe\|4uhe]], [[4uhf\|4uhf]], [[4uhh\|4uhh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhd\|4uhd]], [[4uhe\|4uhe]], [[4uhf\|4uhf]], [[4uhh\|4uhh]]</td></tr>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
		+	[[Category: Dsm 26237]]
		+	[[Category: Large Structures]]
	[[Category: Bonch-Osmolovskaya, E]]		[[Category: Bonch-Osmolovskaya, E]]
	[[Category: Isupov, M N]]		[[Category: Isupov, M N]]

OCA at 16:09, 17 May 2017

2017-05-17T16:09:44Z

←Older revision		Revision as of 16:09, 17 May 2017
Line 1:		Line 1:
		+
	==Structural studies of a thermophilic esterase from Thermogutta terrifontis (Native)==		==Structural studies of a thermophilic esterase from Thermogutta terrifontis (Native)==
	<StructureSection load='4uhc' size='340' side='right' caption='[[4uhc]], [[Resolution\|resolution]] 1.03Å' scene=''>		<StructureSection load='4uhc' size='340' side='right' caption='[[4uhc]], [[Resolution\|resolution]] 1.03Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhd\|4uhd]], [[4uhe\|4uhe]], [[4uhf\|4uhf]], [[4uhh\|4uhh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhd\|4uhd]], [[4uhe\|4uhe]], [[4uhf\|4uhf]], [[4uhh\|4uhh]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uhc RCSB], [http://www.ebi.ac.uk/pdbsum/4uhc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhc OCA], [http://pdbe.org/4uhc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uhc RCSB], [http://www.ebi.ac.uk/pdbsum/4uhc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uhc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4uhc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:01, 10 June 2015

2015-06-10T14:01:13Z

←Older revision		Revision as of 14:01, 10 June 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Structural studies of a thermophilic esterase from Thermogutta terrifontis (Native)==
		+	<StructureSection load='4uhc' size='340' side='right' caption='[[4uhc]], [[Resolution\|resolution]] 1.03Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4uhc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UHC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UHC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4uhd\|4uhd]], [[4uhe\|4uhe]], [[4uhf\|4uhf]], [[4uhh\|4uhh]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uhc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4uhc RCSB], [http://www.ebi.ac.uk/pdbsum/4uhc PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A carboxyl esterase (TtEst) has been identified in a novel thermophilic bacterium, Thermogutta terrifontis from the phylum Planctomycetes and has been cloned and over-expressed in Escherichia coli. The enzyme has been characterised biochemically and shown to have activity towards small p-nitrophenyl (pNP) carboxylic esters with optimal activity for pNP-propionate. The enzyme retained 95% activity after incubation for 1 hour at 80 degrees C. The crystal structures of the native TtEst and its complexes with the substrate analogue, D-malate and the product acetate have been determined to high resolution. The bound ligands have allowed the identification of the carboxyl and alcohol binding pockets in the enzyme active site. Comparison of TtEst with structurally related enzymes has given insight into how differences in their catalytic activity can be rationalised based upon the properties of the amino acid residues in their active site pockets. The mutant enzymes L37A and L251A have been constructed to extend the substrate range of TtEst towards the larger butyrate and valerate pNP-esters. These mutant enzymes have also shown a significant increase in activity towards acetate and propionate pNP esters. A crystal structure of the L37A mutant was determined with the butyrate product bound in the carboxyl pocket of the active site. The mutant structure shows an expansion of the pocket that binds the substrate carboxyl group, which is consistent with the observed increase in activity towards pNP-butyrate. This article is protected by copyright. All rights reserved.

-	~~The entry 4uhc is ON HOLD until Paper Publication~~	+	Structural studies of a thermophilic esterase from a new Planctomycetes species, Thermogutta terrifontis.,Sayer C, Isupov MN, Bonch-Osmolovskaya E, Littlechild JA FEBS J. 2015 May 22. doi: 10.1111/febs.13326. PMID:26011036<ref>PMID:26011036</ref>

-	~~Authors: Sayer~~, C.~~, Isupov, M~~.~~N., Bonch-Osmolovskaya, E., Littlechild, J.A~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Structural studies of a thermophilic esterase from Thermogutta terrifontis (Native)~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
-	[[Category: ~~Isupov, M.N~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Carboxylesterase]]
	[[Category: Bonch-Osmolovskaya, E]]		[[Category: Bonch-Osmolovskaya, E]]
-	[[Category: Littlechild, J.A]]	+	[[Category: Isupov, M N]]
		+	[[Category: Littlechild, J A]]
	[[Category: Sayer, C]]		[[Category: Sayer, C]]
		+	[[Category: Alpha beta hydrolase]]
		+	[[Category: Hydrolase]]

OCA: Protected "4uhc" [edit=sysop:move=sysop]

2015-04-01T09:43:43Z

Protected "4uhc" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:43, 1 April 2015

OCA: New page: '''Unreleased structure''' The entry 4uhc is ON HOLD until Paper Publication Authors: Sayer, C., Isupov, M.N., Bonch-Osmolovskaya, E., Littlechild, J.A. Description: Structural studies...

2015-04-01T09:43:42Z

New page: '''Unreleased structure''' The entry 4uhc is ON HOLD until Paper Publication Authors: Sayer, C., Isupov, M.N., Bonch-Osmolovskaya, E., Littlechild, J.A. Description: Structural studies...

New page

'''Unreleased structure'''

The entry 4uhc is ON HOLD until Paper Publication

Authors: Sayer, C., Isupov, M.N., Bonch-Osmolovskaya, E., Littlechild, J.A.

Description: Structural studies of a thermophilic esterase from Thermogutta terrifontis (Native)
[[Category: Unreleased Structures]]
[[Category: Isupov, M.N]]
[[Category: Bonch-Osmolovskaya, E]]
[[Category: Littlechild, J.A]]
[[Category: Sayer, C]]