4wwf - Revision history

OCA at 11:28, 9 May 2024

2024-05-09T11:28:25Z

←Older revision		Revision as of 11:28, 9 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4wwf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans_CH34 Cupriavidus metallidurans CH34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WWF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wwf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans_CH34 Cupriavidus metallidurans CH34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WWF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwf OCA], [https://pdbe.org/4wwf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wwf RCSB], [https://www.ebi.ac.uk/pdbsum/4wwf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwf OCA], [https://pdbe.org/4wwf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wwf RCSB], [https://www.ebi.ac.uk/pdbsum/4wwf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwf ProSAT]</span></td></tr>
	</table>		</table>

OCA at 21:00, 12 April 2023

2023-04-12T21:00:12Z

←Older revision		Revision as of 21:00, 12 April 2023
Line 3:		Line 3:
	<StructureSection load='4wwf' size='340' side='right'caption='[[4wwf]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='4wwf' size='340' side='right'caption='[[4wwf]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wwf]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cupmc Cupmc~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4WWF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wwf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans_CH34 Cupriavidus metallidurans CH34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WWF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cnrR, cnrX, Rmet_6206, RMe0087 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266264 CUPMC])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwf OCA], [https://pdbe.org/4wwf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wwf RCSB], [https://www.ebi.ac.uk/pdbsum/4wwf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwf ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4wwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwf OCA], [~~http~~://pdbe.org/4wwf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4wwf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4wwf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwf ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~CNRR_RALME CNRR_RALME]~~] CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.	+	[https://www.uniprot.org/uniprot/CNRR_CUPMC CNRR_CUPMC] CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.<ref>PMID:10671463</ref> <ref>PMID:10671464</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cupmc~~]]	+	[[Category: Cupriavidus metallidurans CH34]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Coves, J]]	+	[[Category: Coves J]]
-	[[Category: Grosse, C]]	+	[[Category: Grosse C]]
-	[[Category: Kinnemann, S]]	+	[[Category: Kinnemann S]]
-	[[Category: Maillard~~, A P~~]]	+	[[Category: Maillard AP]]
-	[[Category: Nies~~, D H~~]]	+	[[Category: Nies DH]]
-	[[Category: Petit-Hurtlein, I]]	+	[[Category: Petit-Hurtlein I]]
-	~~[[Category: Rosny, E de~~]]	+	[[Category: Schleuder G]]
-	[[Category: Schleuder, G]]	+	[[Category: Volbeda A]]
-	[[Category: Volbeda, A]]	+	[[Category: De Rosny E]]
-	[[Category: ~~Metal binding protein]]~~	+
-	~~[[Category: Nickel sensor~~]]	+

OCA at 08:06, 23 May 2019

2019-05-23T08:06:43Z

←Older revision		Revision as of 08:06, 23 May 2019
Line 1:		Line 1:

	==High-resolution structure of two Ni-bound forms of the M123C mutant of C. metallidurans CnrXs==		==High-resolution structure of two Ni-bound forms of the M123C mutant of C. metallidurans CnrXs==
-	<StructureSection load='4wwf' size='340' side='right' caption='[[4wwf]], [[Resolution\|resolution]] 1.10Å' scene=''>	+	<StructureSection load='4wwf' size='340' side='right'caption='[[4wwf]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4wwf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WWF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4wwf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupmc Cupmc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WWF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cnrR, cnrX, Rmet_6206, RMe0087 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266264 CUPMC])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwf OCA], [http://pdbe.org/4wwf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wwf RCSB], [http://www.ebi.ac.uk/pdbsum/4wwf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwf OCA], [http://pdbe.org/4wwf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wwf RCSB], [http://www.ebi.ac.uk/pdbsum/4wwf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwf ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cupmc]]
		+	[[Category: Large Structures]]
	[[Category: Coves, J]]		[[Category: Coves, J]]
	[[Category: Grosse, C]]		[[Category: Grosse, C]]

OCA at 00:16, 26 January 2017

2017-01-26T00:16:48Z

←Older revision		Revision as of 00:16, 26 January 2017
Line 1:		Line 1:
		+
	==High-resolution structure of two Ni-bound forms of the M123C mutant of C. metallidurans CnrXs==		==High-resolution structure of two Ni-bound forms of the M123C mutant of C. metallidurans CnrXs==
	<StructureSection load='4wwf' size='340' side='right' caption='[[4wwf]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='4wwf' size='340' side='right' caption='[[4wwf]], [[Resolution\|resolution]] 1.10Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4wwf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WWF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4wwf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WWF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wwf RCSB], [http://www.ebi.ac.uk/pdbsum/4wwf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwf OCA], [http://pdbe.org/4wwf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wwf RCSB], [http://www.ebi.ac.uk/pdbsum/4wwf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4wwf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:53, 12 February 2015

2015-02-12T11:53:13Z

←Older revision		Revision as of 11:53, 12 February 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==High-resolution structure of two Ni-bound forms of the M123C mutant of C. metallidurans CnrXs==
		+	<StructureSection load='4wwf' size='340' side='right' caption='[[4wwf]], [[Resolution\|resolution]] 1.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4wwf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WWF FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wwf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wwf RCSB], [http://www.ebi.ac.uk/pdbsum/4wwf PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CNRR_RALME CNRR_RALME]] CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Resistance to high concentration of nickel ions is mediated in Cupriavidus metallidurans by the CnrCBA transenvelope efflux complex. Expression of the cnrCBA genes is regulated by the transmembrane signal transduction complex CnrYXH. Together, the metal sensor CnrX and the transmembrane antisigma factor CnrY control the availability of the extracytoplasmic function sigma factor CnrH. Release of CnrH from sequestration by CnrY at the cytoplasmic side of the membrane depends essentially on the binding of the agonist metal ion Ni(ii) to the periplasmic metal sensor domain of CnrX. CnrH availability leads to transcription initiation at the promoters cnrYp and cnrCp and to the expression of the genes in the cnrYXHCBA nickel resistance determinant. The first steps of signal propagation by CnrX rely on subtle metal-dependent allosteric modifications. To study the nickel-mediated triggering process by CnrX, we have altered selected residues, F66, M123, and Y135, and explored the physiological consequences of these changes with respect to metal resistance, expression of a cnrCBA-lacZ reporter fusion and protein production. M123C- and Y135F-CnrXs have been further characterized in vitro by metal affinity measurements and crystallographic structure analysis. Atomic-resolution structures of metal-bound M123C- and Y135F-CnrXs showed that Ni(ii) binds two of the three canonical conformations identified and that Ni(ii) sensing likely proceeds by conformation selection.

-	~~The entry 4wwf is ON HOLD~~	+	Response of CnrX from Cupriavidus metallidurans CH34 to nickel binding.,Maillard AP, Kunnemann S, Grosse C, Volbeda A, Schleuder G, Petit-Hartlein I, de Rosny E, Nies DH, Coves J Metallomics. 2015 Jan 28. PMID:25628016<ref>PMID:25628016</ref>

-	~~Authors: Volbeda~~, A.~~, Coves, J., Maillard, A.P., Kinnemann,~~ S.~~, Grosse, C., Schleuder, G., Petit-Hurtlein, I., de Rosny, E., Nies, D.H~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: High-resolution structure of two Ni-bound forms of the M123C mutant of C. metallidurans CnrXs~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Coves, J]]
	[[Category: Grosse, C]]		[[Category: Grosse, C]]
		+	[[Category: Kinnemann, S]]
		+	[[Category: Maillard, A P]]
		+	[[Category: Nies, D H]]
	[[Category: Petit-Hurtlein, I]]		[[Category: Petit-Hurtlein, I]]
		+	[[Category: Rosny, E de]]
	[[Category: Schleuder, G]]		[[Category: Schleuder, G]]
-	[[Category: Coves, J]]
-	[[Category: De Rosny, E]]
-	[[Category: Nies, D.H]]
-	[[Category: Maillard, A.P]]
	[[Category: Volbeda, A]]		[[Category: Volbeda, A]]
-	[[Category: ~~Kinnemann, S~~]]	+	[[Category: Metal binding protein]]
		+	[[Category: Nickel sensor]]

OCA at 20:06, 24 December 2014

2014-12-24T20:06:30Z

←Older revision		Revision as of 20:06, 24 December 2014
Line 6:		Line 6:

	Description: High-resolution structure of two Ni-bound forms of the M123C mutant of C. metallidurans CnrXs		Description: High-resolution structure of two Ni-bound forms of the M123C mutant of C. metallidurans CnrXs
		+	[[Category: Unreleased Structures]]
		+	[[Category: Grosse, C]]
		+	[[Category: Petit-Hurtlein, I]]
		+	[[Category: Schleuder, G]]
		+	[[Category: Coves, J]]
		+	[[Category: De Rosny, E]]
		+	[[Category: Nies, D.H]]
		+	[[Category: Maillard, A.P]]
		+	[[Category: Volbeda, A]]
		+	[[Category: Kinnemann, S]]

OCA: Protected "4wwf" [edit=sysop:move=sysop]

2014-11-19T12:01:05Z

Protected "4wwf" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:01, 19 November 2014

OCA: New page: '''Unreleased structure''' The entry 4wwf is ON HOLD Authors: Volbeda, A., Coves, J., Maillard, A.P., Kinnemann, S., Grosse, C., Schleuder, G., Petit-Hurtlein, I., de Rosny, E., Nies, D...

2014-11-19T12:01:04Z

New page: '''Unreleased structure''' The entry 4wwf is ON HOLD Authors: Volbeda, A., Coves, J., Maillard, A.P., Kinnemann, S., Grosse, C., Schleuder, G., Petit-Hurtlein, I., de Rosny, E., Nies, D...

New page

'''Unreleased structure'''

The entry 4wwf is ON HOLD

Authors: Volbeda, A., Coves, J., Maillard, A.P., Kinnemann, S., Grosse, C., Schleuder, G., Petit-Hurtlein, I., de Rosny, E., Nies, D.H.

Description: High-resolution structure of two Ni-bound forms of the M123C mutant of C. metallidurans CnrXs