4x36 - Revision history

OCA at 21:08, 12 April 2023

2023-04-12T21:08:59Z

←Older revision		Revision as of 21:08, 12 April 2023
Line 3:		Line 3:
	<StructureSection load='4x36' size='340' side='right'caption='[[4x36]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='4x36' size='340' side='right'caption='[[4x36]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4x36]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strpn Strpn~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X36 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4X36 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4x36]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X36 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X36 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:~~ZINC+ION~~'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene~~></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x36 OCA], [https://pdbe.org/4x36 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x36 RCSB], [https://www.ebi.ac.uk/pdbsum/4x36 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x36 ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lytA, SP_1937 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=170187 STRPN])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4x36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x36 OCA], [~~http~~://pdbe.org/4x36 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4x36 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4x36 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4x36 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ALYS_STRPN ALYS_STRPN]] Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Autolysin strictly depends on the presence of choline-containing cell walls for activity.	+	[https://www.uniprot.org/uniprot/ALYS_STRPN ALYS_STRPN] Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Autolysin strictly depends on the presence of choline-containing cell walls for activity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~N-acetylmuramoyl-L-alanine amidase]]~~	+	[[Category: Streptococcus pneumoniae TIGR4]]
-	~~[[Category: Strpn~~]]	+	[[Category: Chen YX]]
-	[[Category: Chen~~, Y X~~]]	+	[[Category: Cheng W]]
-	[[Category: Cheng, W]]	+	[[Category: Li Q]]
-	[[Category: Li, Q]]	+	[[Category: Zhou CZ]]
-	[[Category: Zhou~~, C Z]]~~	+
-	~~[[Category: Amidase]]~~	+
-	~~[[Category: Autolysin]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:51, 29 May 2019

2019-05-29T06:51:35Z

←Older revision		Revision as of 06:51, 29 May 2019
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	==Crystal structure of the autolysin LytA from Streptococcus pneumoniae TIGR4==		==Crystal structure of the autolysin LytA from Streptococcus pneumoniae TIGR4==
-	<StructureSection load='4x36' size='340' side='right' caption='[[4x36]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='4x36' size='340' side='right'caption='[[4x36]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4x36]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X36 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X36 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4x36]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X36 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X36 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lytA, SP_1937 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=170187 STRPN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x36 OCA], [http://pdbe.org/4x36 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x36 RCSB], [http://www.ebi.ac.uk/pdbsum/4x36 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x36 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x36 OCA], [http://pdbe.org/4x36 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x36 RCSB], [http://www.ebi.ac.uk/pdbsum/4x36 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x36 ProSAT]</span></td></tr>
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: N-acetylmuramoyl-L-alanine amidase]]		[[Category: N-acetylmuramoyl-L-alanine amidase]]
		+	[[Category: Strpn]]
	[[Category: Chen, Y X]]		[[Category: Chen, Y X]]
	[[Category: Cheng, W]]		[[Category: Cheng, W]]

OCA at 14:22, 29 March 2017

2017-03-29T14:22:55Z

←Older revision		Revision as of 14:22, 29 March 2017
Line 1:		Line 1:
		+
	==Crystal structure of the autolysin LytA from Streptococcus pneumoniae TIGR4==		==Crystal structure of the autolysin LytA from Streptococcus pneumoniae TIGR4==
	<StructureSection load='4x36' size='340' side='right' caption='[[4x36]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='4x36' size='340' side='right' caption='[[4x36]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x36 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x36 RCSB], [http://www.ebi.ac.uk/pdbsum/4x36 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x36 OCA], [http://pdbe.org/4x36 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x36 RCSB], [http://www.ebi.ac.uk/pdbsum/4x36 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x36 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4x36" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:45, 24 June 2015

2015-06-24T06:45:24Z

←Older revision		Revision as of 06:45, 24 June 2015
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ALYS_STRPN ALYS_STRPN]] Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Autolysin strictly depends on the presence of choline-containing cell walls for activity.		[[http://www.uniprot.org/uniprot/ALYS_STRPN ALYS_STRPN]] Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Autolysin strictly depends on the presence of choline-containing cell walls for activity.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	LytA is responsible for the autolysis of many Streptococcus species, including pathogens such as S. pneumoniae, S. pseudopneumoniae and S. mitis. However, how this major autolysin achieves full activity remains unknown. Here, the full-length structure of the S. pneumoniae LytA dimer is reported at 2.1 A resolution. Each subunit has an N-terminal amidase domain and a C-terminal choline-binding domain consisting of six choline-binding repeats, which form five canonical and one single-layered choline-binding sites. Site-directed mutageneses combined with enzymatic activity assays indicate that dimerization and binding to choline are two independent requirements for the autolytic activity of LytA in vivo. Altogether, it is suggested that dimerization and full occupancy of all choline-binding sites through binding to choline-containing TA chains enable LytA to adopt a fully active conformation which allows the amidase domain to cleave two lactyl-amide bonds located about 103 A apart on the peptidoglycan.
		+
		+	Full-length structure of the major autolysin LytA.,Li Q, Cheng W, Morlot C, Bai XH, Jiang YL, Wang W, Roper DI, Vernet T, Dong YH, Chen Y, Zhou CZ Acta Crystallogr D Biol Crystallogr. 2015 Jun;71(Pt 6):1373-81. doi:, 10.1107/S1399004715007403. Epub 2015 May 23. PMID:26057677<ref>PMID:26057677</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:58, 27 May 2015

2015-05-27T15:58:19Z

←Older revision		Revision as of 15:58, 27 May 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of the autolysin LytA from Streptococcus pneumoniae TIGR4==
-		+	<StructureSection load='4x36' size='340' side='right' caption='[[4x36]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~The entry~~ 4x36 is ~~ON HOLD until Nov 28 2016~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4x36]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X36 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X36 FirstGlance]. <br>
-	~~Authors~~: ~~Cheng, W~~.~~, Li, Q~~.~~, Zhou, C~~.Z.~~, Chen, Y~~.X.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~Description~~: ~~Crystal structure of the autolysin LytA from Streptococcus pneumoniae TIGR4~~	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] </span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x36 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x36 RCSB], [http://www.ebi.ac.uk/pdbsum/4x36 PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Zhou~~, C.Z]]	+	</table>
-	[[~~Category~~: ~~Chen~~, Y.X]]	+	== Function ==
-	[[Category: Li, Q]]	+	[[http://www.uniprot.org/uniprot/ALYS_STRPN ALYS_STRPN]] Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Autolysin strictly depends on the presence of choline-containing cell walls for activity.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: N-acetylmuramoyl-L-alanine amidase]]
		+	[[Category: Chen, Y X]]
	[[Category: Cheng, W]]		[[Category: Cheng, W]]
		+	[[Category: Li, Q]]
		+	[[Category: Zhou, C Z]]
		+	[[Category: Amidase]]
		+	[[Category: Autolysin]]
		+	[[Category: Hydrolase]]

OCA at 20:24, 24 December 2014

2014-12-24T20:24:36Z

←Older revision		Revision as of 20:24, 24 December 2014
Line 6:		Line 6:

	Description: Crystal structure of the autolysin LytA from Streptococcus pneumoniae TIGR4		Description: Crystal structure of the autolysin LytA from Streptococcus pneumoniae TIGR4
		+	[[Category: Unreleased Structures]]
		+	[[Category: Zhou, C.Z]]
		+	[[Category: Chen, Y.X]]
		+	[[Category: Li, Q]]
		+	[[Category: Cheng, W]]

OCA at 11:40, 17 December 2014

2014-12-17T11:40:43Z

←Older revision		Revision as of 11:40, 17 December 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4x36 is ON HOLD	+	The entry 4x36 is ON HOLD until Nov 28 2016

	Authors: Cheng, W., Li, Q., Zhou, C.Z., Chen, Y.X.		Authors: Cheng, W., Li, Q., Zhou, C.Z., Chen, Y.X.

	Description: Crystal structure of the autolysin LytA from Streptococcus pneumoniae TIGR4		Description: Crystal structure of the autolysin LytA from Streptococcus pneumoniae TIGR4

OCA: Protected "4x36" [edit=sysop:move=sysop]

2014-12-10T14:19:04Z

Protected "4x36" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:19, 10 December 2014

OCA: New page: '''Unreleased structure''' The entry 4x36 is ON HOLD Authors: Cheng, W., Li, Q., Zhou, C.Z., Chen, Y.X. Description: Crystal structure of the autolysin LytA from Streptococcus pneumoni...

2014-12-10T14:19:03Z

New page: '''Unreleased structure''' The entry 4x36 is ON HOLD Authors: Cheng, W., Li, Q., Zhou, C.Z., Chen, Y.X. Description: Crystal structure of the autolysin LytA from Streptococcus pneumoni...

New page

'''Unreleased structure'''

The entry 4x36 is ON HOLD

Authors: Cheng, W., Li, Q., Zhou, C.Z., Chen, Y.X.

Description: Crystal structure of the autolysin LytA from Streptococcus pneumoniae TIGR4