4x9p - Revision history

OCA at 07:42, 27 September 2023

2023-09-27T07:42:12Z

←Older revision		Revision as of 07:42, 27 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4x9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X9P FirstGlance]. <br>		<table><tr><td colspan='2'>[[4x9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X9P FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.01Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x9p OCA], [https://pdbe.org/4x9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x9p RCSB], [https://www.ebi.ac.uk/pdbsum/4x9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x9p ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x9p OCA], [https://pdbe.org/4x9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x9p RCSB], [https://www.ebi.ac.uk/pdbsum/4x9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x9p ProSAT]</span></td></tr>
	</table>		</table>

OCA at 21:19, 12 April 2023

2023-04-12T21:19:11Z

←Older revision		Revision as of 21:19, 12 April 2023
Line 3:		Line 3:
	<StructureSection load='4x9p' size='340' side='right'caption='[[4x9p]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='4x9p' size='340' side='right'caption='[[4x9p]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4x9p]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X9P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4X9P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4x9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X9P FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ANXA2, ANX2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x9p OCA], [https://pdbe.org/4x9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x9p RCSB], [https://www.ebi.ac.uk/pdbsum/4x9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x9p ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x9p OCA], [~~http~~://pdbe.org/4x9p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4x9p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4x9p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4x9p ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ANXA2_BOVIN ANXA2_BOVIN]] Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.	+	[https://www.uniprot.org/uniprot/ANXA2_BOVIN ANXA2_BOVIN] Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hollas, H]]	+	[[Category: Hollas H]]
-	[[Category: Kretsinger~~, R H~~]]	+	[[Category: Kretsinger RH]]
-	[[Category: Shumilin~~, I A~~]]	+	[[Category: Shumilin IA]]
-	[[Category: Vedeler, A]]	+	[[Category: Vedeler A]]
-	~~[[Category: Annexin]]~~	+
-	~~[[Category: Calcium binding]]~~	+
-	~~[[Category: Calcium binding protein~~]]	+

OCA at 06:51, 29 May 2019

2019-05-29T06:51:19Z

←Older revision		Revision as of 06:51, 29 May 2019
Line 1:		Line 1:

	==Crystal structure of bovine Annexin A2==		==Crystal structure of bovine Annexin A2==
-	<StructureSection load='4x9p' size='340' side='right' caption='[[4x9p]], [[Resolution\|resolution]] 2.01Å' scene=''>	+	<StructureSection load='4x9p' size='340' side='right'caption='[[4x9p]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4x9p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X9P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X9P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4x9p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X9P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X9P FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ANXA2, ANX2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x9p OCA], [http://pdbe.org/4x9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x9p RCSB], [http://www.ebi.ac.uk/pdbsum/4x9p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x9p ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x9p OCA], [http://pdbe.org/4x9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x9p RCSB], [http://www.ebi.ac.uk/pdbsum/4x9p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x9p ProSAT]</span></td></tr>
	</table>		</table>
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4x9p" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4x9p" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Annexin 3D structures\|Annexin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bovin]]
		+	[[Category: Large Structures]]
	[[Category: Hollas, H]]		[[Category: Hollas, H]]
	[[Category: Kretsinger, R H]]		[[Category: Kretsinger, R H]]
Line 28:		Line 34:
	[[Category: Annexin]]		[[Category: Annexin]]
	[[Category: Calcium binding]]		[[Category: Calcium binding]]
		+	[[Category: Calcium binding protein]]

OCA at 18:35, 1 February 2017

2017-02-01T18:35:20Z

←Older revision		Revision as of 18:35, 1 February 2017
Line 1:		Line 1:
		+
	==Crystal structure of bovine Annexin A2==		==Crystal structure of bovine Annexin A2==
	<StructureSection load='4x9p' size='340' side='right' caption='[[4x9p]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='4x9p' size='340' side='right' caption='[[4x9p]], [[Resolution\|resolution]] 2.01Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4x9p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X9P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X9P FirstGlance]. <br>		<table><tr><td colspan='2'>[[4x9p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X9P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X9P FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x9p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x9p RCSB], [http://www.ebi.ac.uk/pdbsum/4x9p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x9p OCA], [http://pdbe.org/4x9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4x9p RCSB], [http://www.ebi.ac.uk/pdbsum/4x9p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4x9p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
-		+	<div class="pdbe-citations 4x9p" style="background-color:#fffaf0;"></div>
-	==~~See Also==~~	+
-	*[[Annexin\|Annexin]]	+
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:54, 1 April 2015

2015-04-01T06:54:05Z

←Older revision		Revision as of 06:54, 1 April 2015
Line 8:		Line 8:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ANXA2_BOVIN ANXA2_BOVIN]] Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.		[[http://www.uniprot.org/uniprot/ANXA2_BOVIN ANXA2_BOVIN]] Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The progression of aggressive cancer occurs via angiogenesis and metastasis making these processes important targets for the development of anti-cancer agents. However, recent studies have raised the concern that selective inhibition of angiogenesis results in a switch towards increased tumour growth and metastasis. Since Annexin A2 (AnxA2) is involved in both angiogenesis and metastasis, it may serve as an ideal target for the simultaneous inhibition of both processes. Based on the discovery that domains I (DI) and IV (DIV) of AnxA2 are potent inhibitors of angiogenesis, we designed seven peptides derived from these domains based on AnxA2 crystal structures. The peptides were expressed as fusion peptides to increase their folding and solubility. Light scattering, far-UV circular dichroism and thermal transition analyses were employed to investigate their aggregation tendencies, alpha-helical propensity and stability, respectively. 2,2,2-trifluoroethanol (50%) increased the alpha-helical propensities of all peptides, indicating that they may favour a hydrophobic environment, but did not enhance their thermal stability. DI-P2 appears to be the most stable and folded peptide in a hydrophilic environment. The secondary structure of DI-P2 was confirmed by nuclear magnetic resonance spectra. The effect of the seven AnxA2 peptides on the formation and integrity of capillary-like networks was studied in a co-culture system mimicking many of the angiogenesis-related processes. Notably, DI-P2 inhibited significantly network formation in this system, indicating that the folded DI-P2 peptide interferes with vascular endothelial growth factor-dependent pro-angiogenic processes. Thus, this peptide has the potential of being developed further as an anti-angiogenic drug.
		+
		+	The Native Structure of Annexin A2 Peptides in Hydrophilic Environment Determines their Anti-Angiogenic Effects.,Raddum AM, Hollas H, Shumilin I, Henklein P, Kretsinger R, Fossen T, Vedeler A Biochem Pharmacol. 2015 Mar 12. pii: S0006-2952(15)00132-X. doi:, 10.1016/j.bcp.2015.02.013. PMID:25772737<ref>PMID:25772737</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+
		+	==See Also==
		+	*[[Annexin\|Annexin]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 10:47, 28 January 2015

2015-01-28T10:47:17Z

←Older revision		Revision as of 10:47, 28 January 2015
Line 12:		Line 12:
	[[Category: Hollas, H]]		[[Category: Hollas, H]]
	[[Category: Kretsinger, R H]]		[[Category: Kretsinger, R H]]
-	[[Category: Structural genomic]]
	[[Category: Shumilin, I A]]		[[Category: Shumilin, I A]]
	[[Category: Vedeler, A]]		[[Category: Vedeler, A]]
	[[Category: Annexin]]		[[Category: Annexin]]
	[[Category: Calcium binding]]		[[Category: Calcium binding]]
-	[[Category: Nysgrc]]
-	[[Category: Psi-biology]]

OCA at 13:49, 14 January 2015

2015-01-14T13:49:28Z

←Older revision		Revision as of 13:49, 14 January 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Crystal structure of bovine Annexin A2==
-		+	<StructureSection load='4x9p' size='340' side='right' caption='[[4x9p]], [[Resolution\|resolution]] 2.01Å' scene=''>
-	~~The entry~~ 4x9p is ~~ON HOLD~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4x9p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X9P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X9P FirstGlance]. <br>
-	~~Authors~~: ~~Shumilin~~, I.A.~~, Hollas, H~~., ~~Vedeler, A~~., ~~Kretsinger, R~~.H., ~~New York Structural Genomics Research Consortium (NYSGRC)~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x9p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x9p RCSB], [http://www.ebi.ac.uk/pdbsum/4x9p PDBsum]</span></td></tr>
-	~~Description: Crystal structure of bovine Annexin A2~~	+	</table>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ANXA2_BOVIN ANXA2_BOVIN]] Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.
		+	__TOC__
		+	</StructureSection>
	[[Category: Hollas, H]]		[[Category: Hollas, H]]
-	[[Category: ~~Shumilin~~, ~~I.A~~]]	+	[[Category: Kretsinger, R H]]
-	[[Category: ~~Kretsinger~~, ~~R.H~~]]	+	[[Category: Structural genomic]]
		+	[[Category: Shumilin, I A]]
	[[Category: Vedeler, A]]		[[Category: Vedeler, A]]
-	[[Category: ~~New York Structural Genomics Research Consortium (~~Nysgrc)]]	+	[[Category: Annexin]]
		+	[[Category: Calcium binding]]
		+	[[Category: Nysgrc]]
		+	[[Category: Psi-biology]]

OCA at 20:42, 24 December 2014

2014-12-24T20:42:46Z

←Older revision		Revision as of 20:42, 24 December 2014
Line 6:		Line 6:

	Description: Crystal structure of bovine Annexin A2		Description: Crystal structure of bovine Annexin A2
		+	[[Category: Unreleased Structures]]
		+	[[Category: Hollas, H]]
		+	[[Category: Shumilin, I.A]]
		+	[[Category: Kretsinger, R.H]]
		+	[[Category: Vedeler, A]]
		+	[[Category: New York Structural Genomics Research Consortium (Nysgrc)]]

OCA at 10:02, 24 December 2014

2014-12-24T10:02:40Z

←Older revision		Revision as of 10:02, 24 December 2014
Line 3:		Line 3:
	The entry 4x9p is ON HOLD		The entry 4x9p is ON HOLD

-	Authors: Shumilin, I.A., Hollas, H., Vedeler, A., Kretsinger, R.H.	+	Authors: Shumilin, I.A., Hollas, H., Vedeler, A., Kretsinger, R.H., New York Structural Genomics Research Consortium (NYSGRC)

	Description: Crystal structure of bovine Annexin A2		Description: Crystal structure of bovine Annexin A2

OCA: Protected "4x9p" [edit=sysop:move=sysop]

2014-12-17T11:49:16Z

Protected "4x9p" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:49, 17 December 2014