4xdn - Revision history

OCA at 13:00, 1 March 2024

2024-03-01T13:00:31Z

←Older revision		Revision as of 13:00, 1 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4xdn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XDN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XDN FirstGlance]. <br>		<table><tr><td colspan='2'>[[4xdn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XDN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XDN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.08Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xdn OCA], [https://pdbe.org/4xdn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xdn RCSB], [https://www.ebi.ac.uk/pdbsum/4xdn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xdn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xdn OCA], [https://pdbe.org/4xdn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xdn RCSB], [https://www.ebi.ac.uk/pdbsum/4xdn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xdn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/SCC4_YEAST SCC4_YEAST] Involved in sister chromatid cohesion. Forms a complex with SCC2, which is required for the association of the cohesin complex with chromosomes.<ref>PMID:10882066</ref>		[https://www.uniprot.org/uniprot/SCC4_YEAST SCC4_YEAST] Involved in sister chromatid cohesion. Forms a complex with SCC2, which is required for the association of the cohesin complex with chromosomes.<ref>PMID:10882066</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The cohesin ring holds newly replicated sister chromatids together until their separation at anaphase. Initiation of sister chromatid cohesion depends on a separate complex, Scc2NIPBL/Scc4Mau2 (Scc2/4), which loads cohesin onto DNA and determines its localization across the genome. Proper cohesin loading is essential for cell division, and partial defects cause chromosome missegregation and aberrant transcriptional regulation, leading to severe developmental defects in multicellular organisms. We present here a crystal structure showing the interaction between Scc2 and Scc4. Scc4 is a TPR array that envelops an extended Scc2 peptide. Using budding yeast, we demonstrate that a conserved patch on the surface of Scc4 is required to recruit Scc2/4 to centromeres and to build pericentromeric cohesion. These findings reveal the role of Scc4 in determining the localization of cohesin loading and establish a molecular basis for Scc2/4 recruitment to centromeres.
-
-	Structural evidence for Scc4-dependent localization of cohesin loading.,Hinshaw SM, Makrantoni V, Kerr A, Marston AL, Harrison SC Elife. 2015 Jun 3;4. doi: 10.7554/eLife.06057. PMID:26038942<ref>PMID:26038942</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4xdn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:24, 12 April 2023

2023-04-12T21:24:27Z

←Older revision		Revision as of 21:24, 12 April 2023
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	<StructureSection load='4xdn' size='340' side='right'caption='[[4xdn]], [[Resolution\|resolution]] 2.08Å' scene=''>		<StructureSection load='4xdn' size='340' side='right'caption='[[4xdn]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xdn]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XDN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4XDN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xdn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XDN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XDN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), SCC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xdn OCA], [https://pdbe.org/4xdn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xdn RCSB], [https://www.ebi.ac.uk/pdbsum/4xdn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xdn ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4xdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xdn OCA], [~~http~~://pdbe.org/4xdn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4xdn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4xdn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4xdn ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SCC4_YEAST SCC4_YEAST]] Involved in sister chromatid cohesion. Forms a complex with SCC2, which is required for the association of the cohesin complex with chromosomes.<ref>PMID:10882066</ref> [[http://www.uniprot.org/uniprot/SCC2_YEAST SCC2_YEAST]] Plays a structural role in chromatin. Involved in sister chromatid cohesion. Forms a complex with SCC4 required for the stable association of the cohesin complex with chromatin, which may act by hydrolyzing ATP from SMC1 and SMC3 heads. Also participates in DNA repair.<ref>PMID:10882066</ref> <ref>PMID:14614819</ref>	+	[https://www.uniprot.org/uniprot/SCC4_YEAST SCC4_YEAST] Involved in sister chromatid cohesion. Forms a complex with SCC2, which is required for the association of the cohesin complex with chromosomes.<ref>PMID:10882066</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Harrison, S C~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Hinshaw, S M~~]]	+	[[Category: Harrison SC]]
-	[[Category: ~~Cell cycle]]~~	+	[[Category: Hinshaw SM]]
-	~~[[Category: Centromere]]~~	+
-	~~[[Category: Cohesin loading]]~~	+
-	~~[[Category: Cohesion~~]]	+

OCA at 16:04, 20 November 2019

2019-11-20T16:04:39Z

←Older revision		Revision as of 16:04, 20 November 2019
Line 1:		Line 1:

	==Crystal structure of Scc4 in complex with Scc2n==		==Crystal structure of Scc4 in complex with Scc2n==
-	<StructureSection load='4xdn' size='340' side='right' caption='[[4xdn]], [[Resolution\|resolution]] 2.08Å' scene=''>	+	<StructureSection load='4xdn' size='340' side='right'caption='[[4xdn]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4xdn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XDN FirstGlance]. <br>		<table><tr><td colspan='2'>[[4xdn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XDN FirstGlance]. <br>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Harrison, S C]]		[[Category: Harrison, S C]]
	[[Category: Hinshaw, S M]]		[[Category: Hinshaw, S M]]

OCA at 12:16, 6 November 2017

2017-11-06T12:16:01Z

←Older revision		Revision as of 12:16, 6 November 2017
Line 1:		Line 1:
		+
	==Crystal structure of Scc4 in complex with Scc2n==		==Crystal structure of Scc4 in complex with Scc2n==
	<StructureSection load='4xdn' size='340' side='right' caption='[[4xdn]], [[Resolution\|resolution]] 2.08Å' scene=''>		<StructureSection load='4xdn' size='340' side='right' caption='[[4xdn]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xdn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XDN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xdn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XDN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xdn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xdn RCSB], [http://www.ebi.ac.uk/pdbsum/4xdn PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SCC4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), SCC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xdn OCA], [http://pdbe.org/4xdn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xdn RCSB], [http://www.ebi.ac.uk/pdbsum/4xdn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xdn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4xdn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 18824]]
	[[Category: Harrison, S C]]		[[Category: Harrison, S C]]
	[[Category: Hinshaw, S M]]		[[Category: Hinshaw, S M]]

OCA at 14:52, 17 June 2015

2015-06-17T14:52:06Z

←Older revision		Revision as of 14:52, 17 June 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of Scc4 in complex with Scc2n==
		+	<StructureSection load='4xdn' size='340' side='right' caption='[[4xdn]], [[Resolution\|resolution]] 2.08Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4xdn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XDN FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xdn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xdn RCSB], [http://www.ebi.ac.uk/pdbsum/4xdn PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SCC4_YEAST SCC4_YEAST]] Involved in sister chromatid cohesion. Forms a complex with SCC2, which is required for the association of the cohesin complex with chromosomes.<ref>PMID:10882066</ref> [[http://www.uniprot.org/uniprot/SCC2_YEAST SCC2_YEAST]] Plays a structural role in chromatin. Involved in sister chromatid cohesion. Forms a complex with SCC4 required for the stable association of the cohesin complex with chromatin, which may act by hydrolyzing ATP from SMC1 and SMC3 heads. Also participates in DNA repair.<ref>PMID:10882066</ref> <ref>PMID:14614819</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cohesin ring holds newly replicated sister chromatids together until their separation at anaphase. Initiation of sister chromatid cohesion depends on a separate complex, Scc2NIPBL/Scc4Mau2 (Scc2/4), which loads cohesin onto DNA and determines its localization across the genome. Proper cohesin loading is essential for cell division, and partial defects cause chromosome missegregation and aberrant transcriptional regulation, leading to severe developmental defects in multicellular organisms. We present here a crystal structure showing the interaction between Scc2 and Scc4. Scc4 is a TPR array that envelops an extended Scc2 peptide. Using budding yeast, we demonstrate that a conserved patch on the surface of Scc4 is required to recruit Scc2/4 to centromeres and to build pericentromeric cohesion. These findings reveal the role of Scc4 in determining the localization of cohesin loading and establish a molecular basis for Scc2/4 recruitment to centromeres.

-	~~The entry 4xdn is ON HOLD until Paper Publication~~	+	Structural evidence for Scc4-dependent localization of cohesin loading.,Hinshaw SM, Makrantoni V, Kerr A, Marston AL, Harrison SC Elife. 2015 Jun 3;4. doi: 10.7554/eLife.06057. PMID:26038942<ref>PMID:26038942</ref>

-	~~Authors: Hinshaw~~, S.M., Harrison, S.C.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~:	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
-	[[Category: ~~Harrison, S.C~~]]	+	__TOC__
-	[[Category: ~~Hinshaw, S.M~~]]	+	</StructureSection>
		+	[[Category: Harrison, S C]]
		+	[[Category: Hinshaw, S M]]
		+	[[Category: Cell cycle]]
		+	[[Category: Centromere]]
		+	[[Category: Cohesin loading]]
		+	[[Category: Cohesion]]

OCA at 11:57, 25 February 2015

2015-02-25T11:57:21Z

←Older revision		Revision as of 11:57, 25 February 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4xdn is ON HOLD	+	The entry 4xdn is ON HOLD until Paper Publication

	Authors: Hinshaw, S.M., Harrison, S.C.		Authors: Hinshaw, S.M., Harrison, S.C.

OCA: Protected "4xdn" [edit=sysop:move=sysop]

2014-12-31T14:01:27Z

Protected "4xdn" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:01, 31 December 2014

OCA: New page: '''Unreleased structure''' The entry 4xdn is ON HOLD Authors: Hinshaw, S.M., Harrison, S.C. Description: Category: Unreleased Structures Category: Harrison, S.C [[Category: Hi...

2014-12-31T14:01:26Z

New page: '''Unreleased structure''' The entry 4xdn is ON HOLD Authors: Hinshaw, S.M., Harrison, S.C. Description: Category: Unreleased Structures Category: Harrison, S.C [[Category: Hi...

New page

'''Unreleased structure'''

The entry 4xdn is ON HOLD

Authors: Hinshaw, S.M., Harrison, S.C.

Description:
[[Category: Unreleased Structures]]
[[Category: Harrison, S.C]]
[[Category: Hinshaw, S.M]]