4xht - Revision history

OCA at 15:28, 8 November 2023

2023-11-08T15:28:05Z

←Older revision		Revision as of 15:28, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4xht]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XHT FirstGlance]. <br>		<table><tr><td colspan='2'>[[4xht]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XHT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xht OCA], [https://pdbe.org/4xht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xht RCSB], [https://www.ebi.ac.uk/pdbsum/4xht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xht ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.651Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xht OCA], [https://pdbe.org/4xht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xht RCSB], [https://www.ebi.ac.uk/pdbsum/4xht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xht ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 09:59, 19 April 2023

2023-04-19T09:59:27Z

←Older revision		Revision as of 09:59, 19 April 2023
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	<StructureSection load='4xht' size='340' side='right'caption='[[4xht]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='4xht' size='340' side='right'caption='[[4xht]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xht]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XHT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4XHT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xht]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XHT FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xhw\|4xhw]], [[4xhu\|4xhu]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xht OCA], [https://pdbe.org/4xht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xht RCSB], [https://www.ebi.ac.uk/pdbsum/4xht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xht ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIMELESS, TIM, TIM1, TIMELESS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4xht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xht OCA], [~~http~~://pdbe.org/4xht PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4xht RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4xht PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4xht ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TIM_HUMAN TIM_HUMAN]] Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication and in the regulation of the circadian clock. Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock. Negatively regulates CLOCK\|NPAS2-ARTNL/BMAL1\|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Forms a complex with TIPIN and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress. Timeless promotes TIPIN nuclear localization. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.<ref>PMID:17141802</ref> <ref>PMID:17296725</ref> <ref>PMID:23418588</ref> <ref>PMID:9856465</ref>	+	[https://www.uniprot.org/uniprot/TIM_HUMAN TIM_HUMAN] Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication and in the regulation of the circadian clock. Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock. Negatively regulates CLOCK\|NPAS2-ARTNL/BMAL1\|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Forms a complex with TIPIN and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress. Timeless promotes TIPIN nuclear localization. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.<ref>PMID:17141802</ref> <ref>PMID:17296725</ref> <ref>PMID:23418588</ref> <ref>PMID:9856465</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Qian, C]]	+	[[Category: Qian C]]
-	[[Category: Xie, S]]	+	[[Category: Xie S]]
-	~~[[Category: Dna damage response]]~~	+
-	~~[[Category: Replication~~]]	+

OCA at 07:37, 29 May 2019

2019-05-29T07:37:11Z

←Older revision		Revision as of 07:37, 29 May 2019
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		+
	==Crystal structure of Timeless_PAB domain native form==		==Crystal structure of Timeless_PAB domain native form==
-	<StructureSection load='4xht' size='340' side='right' caption='[[4xht]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='4xht' size='340' side='right'caption='[[4xht]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xht]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XHT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xht]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XHT FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xhw\|4xhw]], [[4xhu\|4xhu]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xhw\|4xhw]], [[4xhu\|4xhu]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xht OCA], [http://pdbe.org/4xht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xht RCSB], [http://www.ebi.ac.uk/pdbsum/4xht PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TIMELESS, TIM, TIM1, TIMELESS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xht OCA], [http://pdbe.org/4xht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xht RCSB], [http://www.ebi.ac.uk/pdbsum/4xht PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xht ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Qian, C]]		[[Category: Qian, C]]
	[[Category: Xie, S]]		[[Category: Xie, S]]
	[[Category: Dna damage response]]		[[Category: Dna damage response]]
	[[Category: Replication]]		[[Category: Replication]]

OCA at 14:33, 30 September 2015

2015-09-30T14:33:20Z

←Older revision		Revision as of 14:33, 30 September 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of Timeless_PAB domain native form==
		+	<StructureSection load='4xht' size='340' side='right' caption='[[4xht]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4xht]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XHT FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4xhw\|4xhw]], [[4xhu\|4xhu]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xht OCA], [http://pdbe.org/4xht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xht RCSB], [http://www.ebi.ac.uk/pdbsum/4xht PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TIM_HUMAN TIM_HUMAN]] Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication and in the regulation of the circadian clock. Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock. Negatively regulates CLOCK\|NPAS2-ARTNL/BMAL1\|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Forms a complex with TIPIN and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress. Timeless promotes TIPIN nuclear localization. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules.<ref>PMID:17141802</ref> <ref>PMID:17296725</ref> <ref>PMID:23418588</ref> <ref>PMID:9856465</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human Timeless helps stabilize replication forks during normal DNA replication and plays a critical role in activation of the S phase checkpoint and proper establishment of sister chromatid cohesion. However, it remains elusive whether Timeless is involved in the repair of damaged DNA. Here, we identify that Timeless physically interacts with PARP-1 independent of poly(ADP-ribosyl)ation. We present high-resolution crystal structures of Timeless PAB (PARP-1-binding domain) in free form and in complex with PARP-1 catalytic domain. Interestingly, Timeless PAB domain specifically recognizes PARP-1, but not PARP-2 or PARP-3. Timeless-PARP-1 interaction does not interfere with PARP-1 enzymatic activity. We demonstrate that rapid and transient accumulation of Timeless at laser-induced DNA damage sites requires PARP-1, but not poly(ADP-ribosyl)ation and that Timeless is co-trapped with PARP-1 at DNA lesions upon PARP inhibition. Furthermore, we show that Timeless and PARP-1 interaction is required for efficient homologous recombination repair.

-	~~The entry 4xht is ON HOLD until Paper Publication~~	+	Timeless Interacts with PARP-1 to Promote Homologous Recombination Repair.,Xie S, Mortusewicz O, Ma HT, Herr P, Poon RR, Helleday T, Qian C Mol Cell. 2015 Sep 2. pii: S1097-2765(15)00589-4. doi:, 10.1016/j.molcel.2015.07.031. PMID:26344098<ref>PMID:26344098</ref>

-	~~Authors: Xie~~, S.~~, Qian, C~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Crystal structure of Timeless_PAB domain in SeMet~~-~~labelled form~~	+	<div class="pdbe-citations 4xht" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Unreleased Structures~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Qian, C]]
	[[Category: Xie, S]]		[[Category: Xie, S]]
-	[[Category: ~~Qian, C~~]]	+	[[Category: Dna damage response]]
		+	[[Category: Replication]]

OCA at 15:25, 28 January 2015

2015-01-28T15:25:51Z

←Older revision		Revision as of 15:25, 28 January 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4xht is ON HOLD	+	The entry 4xht is ON HOLD until Paper Publication

	Authors: Xie, S., Qian, C.		Authors: Xie, S., Qian, C.

OCA at 15:52, 21 January 2015

2015-01-21T15:52:23Z

←Older revision		Revision as of 15:52, 21 January 2015
Line 3:		Line 3:
	The entry 4xht is ON HOLD		The entry 4xht is ON HOLD

-	Authors: Xie, S, Qian, C	+	Authors: Xie, S., Qian, C.

-	Description: ~~Structure~~ of ~~Timeless PAB~~ domain	+	Description: Crystal structure of Timeless_PAB domain in SeMet-labelled form
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Xie, S, Qian, C]]	+	[[Category: Xie, S]]
		+	[[Category: Qian, C]]

OCA: Protected "4xht" [edit=sysop:move=sysop]

2015-01-14T12:43:29Z

Protected "4xht" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:43, 14 January 2015

OCA: New page: '''Unreleased structure''' The entry 4xht is ON HOLD Authors: Xie, S, Qian, C Description: Structure of Timeless PAB domain Category: Unreleased Structures [[Category: Xie, S, Qian...

2015-01-14T12:43:28Z

New page: '''Unreleased structure''' The entry 4xht is ON HOLD Authors: Xie, S, Qian, C Description: Structure of Timeless PAB domain Category: Unreleased Structures [[Category: Xie, S, Qian...

New page

'''Unreleased structure'''

The entry 4xht is ON HOLD

Authors: Xie, S, Qian, C

Description: Structure of Timeless PAB domain
[[Category: Unreleased Structures]]
[[Category: Xie, S, Qian, C]]