4xro - Revision history

OCA at 10:50, 10 January 2024

2024-01-10T10:50:41Z

←Older revision		Revision as of 10:50, 10 January 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4xro]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_type_1_(NEW_YORK-5_ISOLATE) Human immunodeficiency virus type 1 (NEW YORK-5 ISOLATE)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XRO FirstGlance]. <br>		<table><tr><td colspan='2'>[[4xro]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_type_1_(NEW_YORK-5_ISOLATE) Human immunodeficiency virus type 1 (NEW YORK-5 ISOLATE)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XRO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xro OCA], [https://pdbe.org/4xro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xro RCSB], [https://www.ebi.ac.uk/pdbsum/4xro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xro ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.01Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xro OCA], [https://pdbe.org/4xro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xro RCSB], [https://www.ebi.ac.uk/pdbsum/4xro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xro ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:48, 26 April 2023

2023-04-26T17:48:50Z

←Older revision		Revision as of 17:48, 26 April 2023
Line 3:		Line 3:
	<StructureSection load='4xro' size='340' side='right'caption='[[4xro]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='4xro' size='340' side='right'caption='[[4xro]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xro]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Hiv~~-1 ~~Hiv~~-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4XRO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xro]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_type_1_(NEW_YORK-5_ISOLATE) Human immunodeficiency virus type 1 (NEW YORK-5 ISOLATE)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XRO FirstGlance]. <br>
-	</td></tr>~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gag ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11698 HIV-1])</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xro OCA], [https://pdbe.org/4xro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xro RCSB], [https://www.ebi.ac.uk/pdbsum/4xro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xro ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4xro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xro OCA], [~~http~~://pdbe.org/4xro PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4xro RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4xro PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4xro ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GAG_HV1N5 GAG_HV1N5]] Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers. p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 (By similarity).	+	[https://www.uniprot.org/uniprot/GAG_HV1N5 GAG_HV1N5] Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers. p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Hiv-1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: James~~, L C~~]]	+	[[Category: James LC]]
-	[[Category: Price~~, A J]]~~	+	[[Category: Price AJ]]
-	~~[[Category: Capsid]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 23:35, 5 June 2019

2019-06-05T23:35:48Z

←Older revision		Revision as of 23:35, 5 June 2019
Line 1:		Line 1:

	==Disulfide stabilized HIV-1 CA hexamer 4mut (S41A, Q67H, V165I, L172I)==		==Disulfide stabilized HIV-1 CA hexamer 4mut (S41A, Q67H, V165I, L172I)==
-	<StructureSection load='4xro' size='340' side='right' caption='[[4xro]], [[Resolution\|resolution]] 2.01Å' scene=''>	+	<StructureSection load='4xro' size='340' side='right'caption='[[4xro]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4xro]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XRO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4xro]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hiv-1 Hiv-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XRO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xro OCA], [http://pdbe.org/4xro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xro RCSB], [http://www.ebi.ac.uk/pdbsum/4xro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xro ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gag ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11698 HIV-1])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xro OCA], [http://pdbe.org/4xro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xro RCSB], [http://www.ebi.ac.uk/pdbsum/4xro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xro ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hiv-1]]
		+	[[Category: Large Structures]]
	[[Category: James, L C]]		[[Category: James, L C]]
	[[Category: Price, A J]]		[[Category: Price, A J]]
	[[Category: Capsid]]		[[Category: Capsid]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA at 16:10, 17 May 2017

2017-05-17T16:10:49Z

←Older revision		Revision as of 16:10, 17 May 2017
Line 1:		Line 1:
		+
	==Disulfide stabilized HIV-1 CA hexamer 4mut (S41A, Q67H, V165I, L172I)==		==Disulfide stabilized HIV-1 CA hexamer 4mut (S41A, Q67H, V165I, L172I)==
	<StructureSection load='4xro' size='340' side='right' caption='[[4xro]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='4xro' size='340' side='right' caption='[[4xro]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4xro]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XRO FirstGlance]. <br>		<table><tr><td colspan='2'>[[4xro]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XRO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xro OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xro RCSB], [http://www.ebi.ac.uk/pdbsum/4xro PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xro OCA], [http://pdbe.org/4xro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xro RCSB], [http://www.ebi.ac.uk/pdbsum/4xro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xro ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4xro" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:26, 15 July 2015

2015-07-15T13:26:37Z

←Older revision		Revision as of 13:26, 15 July 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Disulfide stabilized HIV-1 CA hexamer 4mut (S41A, Q67H, V165I, L172I)==
		+	<StructureSection load='4xro' size='340' side='right' caption='[[4xro]], [[Resolution\|resolution]] 2.01Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4xro]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XRO FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xro OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xro RCSB], [http://www.ebi.ac.uk/pdbsum/4xro PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GAG_HV1N5 GAG_HV1N5]] Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers. p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During HIV-1 infection of cells, the viral capsid plays critical roles in reverse transcription and nuclear entry of the virus. The capsid-targeting small molecule PF74 inhibits HIV-1 at early stages of infection. HIV-1 resistance to PF74 is complex, requiring multiple amino acid substitutions in the viral CA protein. Here we report the identification and analysis of a novel PF74-resistant mutant encoding amino acid changes in both domains of CA, three of which are near the pocket where PF74 binds. Interestingly, the mutant virus retained partial PF74 binding, and its replication was stimulated by the compound. The mutant capsid structure was not significantly perturbed by binding of PF74; rather, the mutations inhibited capsid interactions with CPSF6 and Nup153 and altered HIV-1 dependence on these host factors and on TNPO3. Moreover, the replication of the mutant virus was markedly impaired in activated primary CD4+ T cells and macrophages. Our results suggest that HIV-1 escapes a capsid-targeting small molecule inhibitor by altering the virus's dependence on host factors normally required for entry into the nucleus. They further imply that clinical resistance to inhibitors targeting the PF74 binding pocket is likely to be strongly limited by functional constraints on HIV-1 evolution. IMPORTANCE: The HIV-1 capsid plays critical roles in early steps of infection and is an attractive target for therapy. Here we show that selection for resistance to a capsid-targeting small molecule inhibitor can result in viral dependence on the compound. The mutant virus was debilitated in primary T cells and macrophages---cellular targets of infection in vivo. The mutations also altered the virus's dependence on cellular factors that are normally required for HIV-1 entry into the nucleus. This work provides new information regarding mechanisms of HIV-1 resistance that should be useful in efforts to develop clinically useful drugs targeting the HIV-1 capsid.

-	~~The entry 4xro is ON HOLD until Paper Publication~~	+	HIV-1 Resistance to the Capsid-Targeting Inhibitor PF74 Results in Altered Dependence on Host Factors Required for Virus Nuclear Entry.,Zhou J, Price AJ, Halambage UD, James LC, Aiken C J Virol. 2015 Jun 24. pii: JVI.00340-15. PMID:26109731<ref>PMID:26109731</ref>

-	~~Authors: Price~~, A.J.~~, James, L.C~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description:~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
-	[[Category: James, L.C]]	+	__TOC__
-	[[Category: Price, A.J]]	+	</StructureSection>
		+	[[Category: James, L C]]
		+	[[Category: Price, A J]]
		+	[[Category: Capsid]]
		+	[[Category: Viral protein]]

OCA: Protected "4xro" [edit=sysop:move=sysop]

2015-01-28T15:45:19Z

Protected "4xro" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:45, 28 January 2015

OCA: New page: '''Unreleased structure''' The entry 4xro is ON HOLD until Paper Publication Authors: Price, A.J., James, L.C. Description: Category: Unreleased Structures [[Category: James, L.C]...

2015-01-28T15:45:18Z

New page: '''Unreleased structure''' The entry 4xro is ON HOLD until Paper Publication Authors: Price, A.J., James, L.C. Description: Category: Unreleased Structures [[Category: James, L.C]...

New page

'''Unreleased structure'''

The entry 4xro is ON HOLD until Paper Publication

Authors: Price, A.J., James, L.C.

Description:
[[Category: Unreleased Structures]]
[[Category: James, L.C]]
[[Category: Price, A.J]]