4ybg - Revision history

OCA at 09:03, 20 March 2024

2024-03-20T09:03:29Z

←Older revision		Revision as of 09:03, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ybg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YBG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ybg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YBG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.602Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ybg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ybg OCA], [https://pdbe.org/4ybg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ybg RCSB], [https://www.ebi.ac.uk/pdbsum/4ybg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ybg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ybg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ybg OCA], [https://pdbe.org/4ybg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ybg RCSB], [https://www.ebi.ac.uk/pdbsum/4ybg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ybg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/MAEL_DROME MAEL_DROME] Involved both in the piRNA and miRNA metabolic processes. As a component of the meiotic nuage, plays a central role during oogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Repression of transposable elements is mediated via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the repression of transposons. As a nuclear component, it is required for proper differentiation in the germline stem cell (GSC) lineage by repressing microRNA-7 (miR-7), thereby acting as an indirect regulator of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene and repressing its expression; miR-7 repression alleviates the Bam repression by miR-7, thereby allowing differentiation in the germline stem cell (GSC) lineage. Indirectly required to position the microtubule organizing center in stage 2-6 oocytes.<ref>PMID:11277405</ref> <ref>PMID:12538514</ref> <ref>PMID:17428915</ref> <ref>PMID:19758565</ref> <ref>PMID:23159368</ref>		[https://www.uniprot.org/uniprot/MAEL_DROME MAEL_DROME] Involved both in the piRNA and miRNA metabolic processes. As a component of the meiotic nuage, plays a central role during oogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Repression of transposable elements is mediated via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the repression of transposons. As a nuclear component, it is required for proper differentiation in the germline stem cell (GSC) lineage by repressing microRNA-7 (miR-7), thereby acting as an indirect regulator of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene and repressing its expression; miR-7 repression alleviates the Bam repression by miR-7, thereby allowing differentiation in the germline stem cell (GSC) lineage. Indirectly required to position the microtubule organizing center in stage 2-6 oocytes.<ref>PMID:11277405</ref> <ref>PMID:12538514</ref> <ref>PMID:17428915</ref> <ref>PMID:19758565</ref> <ref>PMID:23159368</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	PIWI-interacting RNAs (piRNAs) protect the genome from transposons in animal gonads. Maelstrom (Mael) is an evolutionarily conserved protein, composed of a high-mobility group (HMG) domain and a MAEL domain, and is essential for piRNA-mediated transcriptional transposon silencing in various species, such as Drosophila and mice. However, its structure and biochemical function have remained elusive. Here, we report the crystal structure of the MAEL domain from Drosophila melanogaster Mael, at 1.6 A resolution. The structure reveals that the MAEL domain has an RNase H-like fold but lacks canonical catalytic residues conserved among RNase H-like superfamily nucleases. Our biochemical analyses reveal that the MAEL domain exhibits single-stranded RNA (ssRNA)-specific endonuclease activity. Our cell-based analyses further indicate that ssRNA cleavage activity appears dispensable for piRNA-mediated transcriptional transposon silencing in Drosophila. Our findings provide clues toward understanding the multiple roles of Mael in the piRNA pathway.
-
-	Crystal Structure and Activity of the Endoribonuclease Domain of the piRNA Pathway Factor Maelstrom.,Matsumoto N, Sato K, Nishimasu H, Namba Y, Miyakubi K, Dohmae N, Ishitani R, Siomi H, Siomi MC, Nureki O Cell Rep. 2015 Apr 21;11(3):366-75. doi: 10.1016/j.celrep.2015.03.030. Epub 2015 , Apr 9. PMID:25865890<ref>PMID:25865890</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4ybg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:06, 3 May 2023

2023-05-03T08:06:51Z

←Older revision		Revision as of 08:06, 3 May 2023
Line 3:		Line 3:
	<StructureSection load='4ybg' size='340' side='right'caption='[[4ybg]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4ybg' size='340' side='right'caption='[[4ybg]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ybg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YBG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4YBG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ybg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YBG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mael, CG11254 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ybg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ybg OCA], [https://pdbe.org/4ybg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ybg RCSB], [https://www.ebi.ac.uk/pdbsum/4ybg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ybg ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ybg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ybg OCA], [~~http~~://pdbe.org/4ybg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ybg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ybg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ybg ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MAEL_DROME MAEL_DROME]] Involved both in the piRNA and miRNA metabolic processes. As a component of the meiotic nuage, plays a central role during oogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Repression of transposable elements is mediated via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the repression of transposons. As a nuclear component, it is required for proper differentiation in the germline stem cell (GSC) lineage by repressing microRNA-7 (miR-7), thereby acting as an indirect regulator of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene and repressing its expression; miR-7 repression alleviates the Bam repression by miR-7, thereby allowing differentiation in the germline stem cell (GSC) lineage. Indirectly required to position the microtubule organizing center in stage 2-6 oocytes.<ref>PMID:11277405</ref> <ref>PMID:12538514</ref> <ref>PMID:17428915</ref> <ref>PMID:19758565</ref> <ref>PMID:23159368</ref>	+	[https://www.uniprot.org/uniprot/MAEL_DROME MAEL_DROME] Involved both in the piRNA and miRNA metabolic processes. As a component of the meiotic nuage, plays a central role during oogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Repression of transposable elements is mediated via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the repression of transposons. As a nuclear component, it is required for proper differentiation in the germline stem cell (GSC) lineage by repressing microRNA-7 (miR-7), thereby acting as an indirect regulator of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene and repressing its expression; miR-7 repression alleviates the Bam repression by miR-7, thereby allowing differentiation in the germline stem cell (GSC) lineage. Indirectly required to position the microtubule organizing center in stage 2-6 oocytes.<ref>PMID:11277405</ref> <ref>PMID:12538514</ref> <ref>PMID:17428915</ref> <ref>PMID:19758565</ref> <ref>PMID:23159368</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drome~~]]	+	[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ishitani, R]]	+	[[Category: Ishitani R]]
-	[[Category: Matsumoto, N]]	+	[[Category: Matsumoto N]]
-	[[Category: Nishimasu, H]]	+	[[Category: Nishimasu H]]
-	[[Category: Nureki, O]]	+	[[Category: Nureki O]]
-	~~[[Category: Endoribonuclease]]~~	+
-	~~[[Category: Gene silencing]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Ribonuclease h-like fold]]~~	+
-	~~[[Category: Transposon]]~~	+
-	~~[[Category: Zinc~~]]	+

OCA at 07:13, 12 June 2019

2019-06-12T07:13:04Z

←Older revision		Revision as of 07:13, 12 June 2019
Line 1:		Line 1:

	==Crystal structure of the MAEL domain of Drosophila melanogaster Maelstrom==		==Crystal structure of the MAEL domain of Drosophila melanogaster Maelstrom==
-	<StructureSection load='4ybg' size='340' side='right' caption='[[4ybg]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='4ybg' size='340' side='right'caption='[[4ybg]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ybg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YBG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ybg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YBG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mael, CG11254 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ybg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ybg OCA], [http://pdbe.org/4ybg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ybg RCSB], [http://www.ebi.ac.uk/pdbsum/4ybg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ybg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ybg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ybg OCA], [http://pdbe.org/4ybg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ybg RCSB], [http://www.ebi.ac.uk/pdbsum/4ybg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ybg ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Drome]]
		+	[[Category: Large Structures]]
	[[Category: Ishitani, R]]		[[Category: Ishitani, R]]
	[[Category: Matsumoto, N]]		[[Category: Matsumoto, N]]

OCA at 01:54, 10 March 2017

2017-03-10T01:54:34Z

←Older revision		Revision as of 01:54, 10 March 2017
Line 1:		Line 1:
		+
	==Crystal structure of the MAEL domain of Drosophila melanogaster Maelstrom==		==Crystal structure of the MAEL domain of Drosophila melanogaster Maelstrom==
	<StructureSection load='4ybg' size='340' side='right' caption='[[4ybg]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4ybg' size='340' side='right' caption='[[4ybg]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4ybg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YBG FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ybg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YBG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ybg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ybg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ybg RCSB], [http://www.ebi.ac.uk/pdbsum/4ybg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ybg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ybg OCA], [http://pdbe.org/4ybg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ybg RCSB], [http://www.ebi.ac.uk/pdbsum/4ybg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ybg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ybg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:38, 30 April 2015

2015-04-30T11:38:00Z

←Older revision		Revision as of 11:38, 30 April 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the MAEL domain of Drosophila melanogaster Maelstrom==
		+	<StructureSection load='4ybg' size='340' side='right' caption='[[4ybg]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ybg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YBG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YBG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ybg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ybg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ybg RCSB], [http://www.ebi.ac.uk/pdbsum/4ybg PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MAEL_DROME MAEL_DROME]] Involved both in the piRNA and miRNA metabolic processes. As a component of the meiotic nuage, plays a central role during oogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Repression of transposable elements is mediated via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the repression of transposons. As a nuclear component, it is required for proper differentiation in the germline stem cell (GSC) lineage by repressing microRNA-7 (miR-7), thereby acting as an indirect regulator of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene and repressing its expression; miR-7 repression alleviates the Bam repression by miR-7, thereby allowing differentiation in the germline stem cell (GSC) lineage. Indirectly required to position the microtubule organizing center in stage 2-6 oocytes.<ref>PMID:11277405</ref> <ref>PMID:12538514</ref> <ref>PMID:17428915</ref> <ref>PMID:19758565</ref> <ref>PMID:23159368</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	PIWI-interacting RNAs (piRNAs) protect the genome from transposons in animal gonads. Maelstrom (Mael) is an evolutionarily conserved protein, composed of a high-mobility group (HMG) domain and a MAEL domain, and is essential for piRNA-mediated transcriptional transposon silencing in various species, such as Drosophila and mice. However, its structure and biochemical function have remained elusive. Here, we report the crystal structure of the MAEL domain from Drosophila melanogaster Mael, at 1.6 A resolution. The structure reveals that the MAEL domain has an RNase H-like fold but lacks canonical catalytic residues conserved among RNase H-like superfamily nucleases. Our biochemical analyses reveal that the MAEL domain exhibits single-stranded RNA (ssRNA)-specific endonuclease activity. Our cell-based analyses further indicate that ssRNA cleavage activity appears dispensable for piRNA-mediated transcriptional transposon silencing in Drosophila. Our findings provide clues toward understanding the multiple roles of Mael in the piRNA pathway.

-	~~The entry 4ybg is ON HOLD until Paper Publication~~	+	Crystal Structure and Activity of the Endoribonuclease Domain of the piRNA Pathway Factor Maelstrom.,Matsumoto N, Sato K, Nishimasu H, Namba Y, Miyakubi K, Dohmae N, Ishitani R, Siomi H, Siomi MC, Nureki O Cell Rep. 2015 Apr 21;11(3):366-75. doi: 10.1016/j.celrep.2015.03.030. Epub 2015 , Apr 9. PMID:25865890<ref>PMID:25865890</ref>

-	~~Authors: Matsumoto~~, N.~~, Ishitani, R~~.~~, Nishimasu, H., Nureki, O~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of the MAEL domain of Drosophila melanogaster Maelstrom~~	+	== References ==
-	[[Category: ~~Unreleased Structures~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ishitani, R]]
		+	[[Category: Matsumoto, N]]
		+	[[Category: Nishimasu, H]]
	[[Category: Nureki, O]]		[[Category: Nureki, O]]
-	[[Category: ~~Nishimasu, H~~]]	+	[[Category: Endoribonuclease]]
-	[[Category: ~~Matsumoto, N~~]]	+	[[Category: Gene silencing]]
-	[[Category: ~~Ishitani, R~~]]	+	[[Category: Hydrolase]]
		+	[[Category: Ribonuclease h-like fold]]
		+	[[Category: Transposon]]
		+	[[Category: Zinc]]

OCA at 10:28, 11 March 2015

2015-03-11T10:28:11Z

←Older revision		Revision as of 10:28, 11 March 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4ybg is ON HOLD	+	The entry 4ybg is ON HOLD until Paper Publication

	Authors: Matsumoto, N., Ishitani, R., Nishimasu, H., Nureki, O.		Authors: Matsumoto, N., Ishitani, R., Nishimasu, H., Nureki, O.

OCA: Protected "4ybg" [edit=sysop:move=sysop]

2015-02-25T12:56:42Z

Protected "4ybg" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:56, 25 February 2015

OCA: New page: '''Unreleased structure''' The entry 4ybg is ON HOLD Authors: Matsumoto, N., Ishitani, R., Nishimasu, H., Nureki, O. Description: Crystal structure of the MAEL domain of Drosophila mel...

2015-02-25T12:56:41Z

New page: '''Unreleased structure''' The entry 4ybg is ON HOLD Authors: Matsumoto, N., Ishitani, R., Nishimasu, H., Nureki, O. Description: Crystal structure of the MAEL domain of Drosophila mel...

New page

'''Unreleased structure'''

The entry 4ybg is ON HOLD

Authors: Matsumoto, N., Ishitani, R., Nishimasu, H., Nureki, O.

Description: Crystal structure of the MAEL domain of Drosophila melanogaster Maelstrom
[[Category: Unreleased Structures]]
[[Category: Nureki, O]]
[[Category: Nishimasu, H]]
[[Category: Matsumoto, N]]
[[Category: Ishitani, R]]