4yol - Revision history

OCA at 08:22, 3 May 2023

2023-05-03T08:22:54Z

←Older revision		Revision as of 08:22, 3 May 2023
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	==Human fibroblast growth factor-1 C16S/A66C/C117A/P134A==		==Human fibroblast growth factor-1 C16S/A66C/C117A/P134A==
-	<StructureSection load='4yol' size='340' side='right' caption='[[4yol]], [[Resolution\|resolution]] 1.97Å' scene=''>	+	<StructureSection load='4yol' size='340' side='right'caption='[[4yol]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4yol]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YOL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4YOL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4yol]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YOL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2afg\|2afg]], [[1jqz\|1jqz]], [[1rg8\|1rg8]], [[4q9g\|4q9g]], [[4qal\|4qal]], [[3hom\|3hom]], [[3fjk\|3fjk]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yol OCA], [https://pdbe.org/4yol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yol RCSB], [https://www.ebi.ac.uk/pdbsum/4yol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yol ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FGF1, FGFA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4yol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yol OCA], [~~http~~://pdbe.org/4yol PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4yol RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4yol PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4yol ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FGF1_HUMAN FGF1_HUMAN]] Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.<ref>PMID:8663044</ref> <ref>PMID:16597617</ref> <ref>PMID:20145243</ref>	+	[https://www.uniprot.org/uniprot/FGF1_HUMAN FGF1_HUMAN] Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.<ref>PMID:8663044</ref> <ref>PMID:16597617</ref> <ref>PMID:20145243</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
-	*[[Fibroblast growth factor\|Fibroblast growth factor]]	+	*[[Fibroblast growth factor 3D structures\|Fibroblast growth factor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Blaber, M~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Xia, X~~]]	+	[[Category: Blaber M]]
-	[[Category: ~~Cysteine-free mutant]]~~	+	[[Category: Xia X]]
-	~~[[Category: Fgf-1]]~~	+
-	~~[[Category: Fibroblast growth factor-1]]~~	+
-	~~[[Category: Intramolecular disulfide]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 09:39, 13 February 2019

2019-02-13T09:39:15Z

←Older revision		Revision as of 09:39, 13 February 2019
Line 3:		Line 3:
	<StructureSection load='4yol' size='340' side='right' caption='[[4yol]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='4yol' size='340' side='right' caption='[[4yol]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4yol]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YOL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4yol]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YOL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2afg\|2afg]], [[1jqz\|1jqz]], [[1rg8\|1rg8]], [[4q9g\|4q9g]], [[4qal\|4qal]], [[3hom\|3hom]], [[3fjk\|3fjk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2afg\|2afg]], [[1jqz\|1jqz]], [[1rg8\|1rg8]], [[4q9g\|4q9g]], [[4qal\|4qal]], [[3hom\|3hom]], [[3fjk\|3fjk]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yol OCA], [http://pdbe.org/4yol PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yol RCSB], [http://www.ebi.ac.uk/pdbsum/4yol PDBsum]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FGF1, FGFA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yol OCA], [http://pdbe.org/4yol PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yol RCSB], [http://www.ebi.ac.uk/pdbsum/4yol PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yol ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4yol" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4yol" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Fibroblast growth factor\|Fibroblast growth factor]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Blaber, M]]		[[Category: Blaber, M]]
	[[Category: Xia, X]]		[[Category: Xia, X]]

OCA at 17:54, 10 May 2016

2016-05-10T17:54:53Z

←Older revision		Revision as of 17:54, 10 May 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 4yol is ~~ON HOLD~~ ~~until Mar 11 2017~~	+	==Human fibroblast growth factor-1 C16S/A66C/C117A/P134A==
		+	<StructureSection load='4yol' size='340' side='right' caption='[[4yol]], [[Resolution\|resolution]] 1.97Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4yol]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YOL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2afg\|2afg]], [[1jqz\|1jqz]], [[1rg8\|1rg8]], [[4q9g\|4q9g]], [[4qal\|4qal]], [[3hom\|3hom]], [[3fjk\|3fjk]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yol OCA], [http://pdbe.org/4yol PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yol RCSB], [http://www.ebi.ac.uk/pdbsum/4yol PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FGF1_HUMAN FGF1_HUMAN]] Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.<ref>PMID:8663044</ref> <ref>PMID:16597617</ref> <ref>PMID:20145243</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human fibroblast growth factor-1 (FGF-1) has broad therapeutic potential in regenerative medicine but has undesirable biophysical properties of low thermostability and 3 buried cysteine (Cys) residues (at positions 16, 83, and 117) that interact to promote irreversible protein unfolding under oxidizing conditions. Mutational substitution of such Cys residues eliminates reactive buried thiols but cannot be accomplished simultaneously at all 3 positions without also introducing further substantial instability. The mutational introduction of a novel Cys residue (Ala66Cys) that forms a stabilizing disulfide bond (i.e., cystine) with one of the extant Cys residues (Cys83) effectively eliminates one Cys while increasing overall stability. This increase in stability offsets the associated instability of remaining Cys substitution mutations and permits production of a Cys-free form of FGF-1 (Cys16Ser/Ala66Cys/Cys117Ala) with only minor overall instability. The addition of a further stabilizing mutation (Pro134Ala) creates a Cys-free FGF-1 mutant with essentially wild-type biophysical properties. The elimination of buried free thiols in FGF-1 can substantially increase the protein half-life in cell culture. Here, we show that the effective cell survival/mitogenic functional activity of a fully Cys-free form is also substantially increased and is equivalent to wild-type FGF-1 formulated in the presence of heparin sulfate as a stabilizing agent. The results identify this Cys-free FGF-1 mutant as an advantageous "second generation" form of FGF-1 for therapeutic application.

-	~~Authors: Xia~~, X., Blaber, M.	+	Engineering a Cysteine-Free Form of Human Fibroblast Growth Factor-1 for "Second Generation" Therapeutic Application.,Xia X, Kumru OS, Blaber SI, Middaugh CR, Li L, Ornitz DM, Sutherland MA, Tenorio CA, Blaber M J Pharm Sci. 2016 Apr;105(4):1444-53. doi: 10.1016/j.xphs.2016.02.010. PMID:27019961<ref>PMID:27019961</ref>

-	~~Description: Human fibroblast growth factor-1 C16S~~/~~A66C~~/~~C117A/P134A~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 4yol" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Blaber, M]]		[[Category: Blaber, M]]
	[[Category: Xia, X]]		[[Category: Xia, X]]
		+	[[Category: Cysteine-free mutant]]
		+	[[Category: Fgf-1]]
		+	[[Category: Fibroblast growth factor-1]]
		+	[[Category: Intramolecular disulfide]]
		+	[[Category: Protein binding]]

OCA at 10:04, 13 May 2015

2015-05-13T10:04:32Z

←Older revision		Revision as of 10:04, 13 May 2015
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4yol is ON HOLD	+	The entry 4yol is ON HOLD until Mar 11 2017

	Authors: Xia, X., Blaber, M.		Authors: Xia, X., Blaber, M.

OCA at 12:38, 26 March 2015

2015-03-26T12:38:37Z

←Older revision		Revision as of 12:38, 26 March 2015
Line 3:		Line 3:
	The entry 4yol is ON HOLD		The entry 4yol is ON HOLD

-	Authors: Xia, X	+	Authors: Xia, X., Blaber, M.

-	Description: ~~human~~ fibroblast growth factor-1 C16S/A66C/C117A/P134A	+	Description: Human fibroblast growth factor-1 C16S/A66C/C117A/P134A
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Blaber, M]]
	[[Category: Xia, X]]		[[Category: Xia, X]]

OCA: Protected "4yol" [edit=sysop:move=sysop]

2015-03-18T10:58:02Z

Protected "4yol" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:58, 18 March 2015

OCA: New page: '''Unreleased structure''' The entry 4yol is ON HOLD Authors: Xia, X Description: human fibroblast growth factor-1 C16S/A66C/C117A/P134A Category: Unreleased Structures [[Category:...

2015-03-18T10:58:01Z

New page: '''Unreleased structure''' The entry 4yol is ON HOLD Authors: Xia, X Description: human fibroblast growth factor-1 C16S/A66C/C117A/P134A Category: Unreleased Structures [[Category:...

New page

'''Unreleased structure'''

The entry 4yol is ON HOLD

Authors: Xia, X

Description: human fibroblast growth factor-1 C16S/A66C/C117A/P134A
[[Category: Unreleased Structures]]
[[Category: Xia, X]]